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The map label for this gene is 148267353

Identifier: 148267353

GI number: 148267353

Start: 966648

End: 967715

Strand: Direct

Name: 148267353

Synonym: SaurJH9_0919

Alternate gene names: NA

Gene position: 966648-967715 (Clockwise)

Preceding gene: 148267352

Following gene: 148267354

Centisome position: 33.26

GC content: 37.27

Gene sequence:

>1068_bases
ATGTGGAATAAGAATCGACTTACTCAAATGTTAAGTATTGAATATCCAATTATACAAGCAGGTATGGCAGGAAGTACGAC
ACCGAAATTAGTTGCATCAGTAAGTAACAGTGGTGGGTTAGGCACAATAGGCGCAGGTTACTTTAATACGCAGCAATTGG
AAGATGAAATAGATTATGTACGCCAATTAACGTCAAATTCTTTTGGCGTAAATGTCTTTGTACCAAGTCAACAATCATAT
ACCAGTAGTCAAATTGAAAATATGAATGCATGGTTAAAACCTTATCGACGCGCATTACATTTAGAAGAGCCGGTTGTAAA
AATTATCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCCTGTATGTTGTTTTACTTTTG
GAATTCCAAATGAATCTATTATAGAAAGATTGAAAGAAGCAAACATTAAGCTAATAGGTACAGCAACAAGTGTTGATGAA
GCTATTGCGAATGAAAAAGCGGGTATGGATGCTATCGTTGCTCAAGGTAGTGAAGCAGGTGGACATCGTGGTTCATTTTT
AAAACCTAAAAATCAATTACCTATGGTTGGAACAATATCTTTAGTGCCACAAATTGTAGATGTCGTTTCAATTCCGGTCA
TTGCCGCTGGTGGAATTATGGACGGTAGAGGAGTTTTGGCAAGTATTGTCTTAGGTGCAGAAGGGGTACAAATGGGCACT
GCATTTTTAACATCACAAGATAGTAATGCATCAGAACTACTACGAGACGCAATTATAAATAGTAAAGAAACAGATACAGT
CGTTACAAAAGCGTTTAGTGGAAAGCTTGCACGCGGTATCAACAATAGGTTTATCGAAGAAATGTCCCAATACGAAGGCG
ATATCCCAGATTATCCAATACAAAATGAGCTAACAAGTAGCATAAGAAAAGCCGCAGCAAACATCGGCGACAAAGAGTTA
ACACATATGTGGAGTGGACAAAGCCCGCGACTAGCAACAACGCATCCCGCCAACACCATCATGTCCAATATAATCAATCA
AATTAATCAAATCATGCAATATAAATAA

Upstream 100 bases:

>100_bases
GATGAGATGGATAAAAAGGAAAAAGAAAAACTTTCTCAACAGCAAATTCAATTTCAACAACGGAAAAATCGCAACGTATC
TATATAAGGAGCGAACAGCT

Downstream 100 bases:

>100_bases
TCGACCGCAATCCACAAAAGCACAAGCACTCCCAAACATTATTTTAGTGCTTGCCATTTTTGTGGATTGCGTTTCTATTT
TACCAATCTAGTCAAACGAA

Product: 2-nitropropane dioxygenase, NPD

Products: NA

Alternate protein names: Nitroalkane oxidase

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIGAGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSY
TSSQIENMNAWLKPYRRALHLEEPVVKIIEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDE
AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGGIMDGRGVLASIVLGAEGVQMGT
AFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKLARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKEL
THMWSGQSPRLATTHPANTIMSNIINQINQIMQYK

Sequences:

>Translated_355_residues
MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIGAGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSY
TSSQIENMNAWLKPYRRALHLEEPVVKIIEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDE
AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGGIMDGRGVLASIVLGAEGVQMGT
AFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKLARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKEL
THMWSGQSPRLATTHPANTIMSNIINQINQIMQYK
>Mature_355_residues
MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIGAGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSY
TSSQIENMNAWLKPYRRALHLEEPVVKIIEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDE
AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGGIMDGRGVLASIVLGAEGVQMGT
AFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKLARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKEL
THMWSGQSPRLATTHPANTIMSNIINQINQIMQYK

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=349, Percent_Identity=30.3724928366762, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): 2NPD_STAA2 (A6U025)

Other databases:

- EMBL:   CP000736
- RefSeq:   YP_001316080.1
- ProteinModelPortal:   A6U025
- SMR:   A6U025
- STRING:   A6U025
- EnsemblBacteria:   EBSTAT00000015499
- GeneID:   5315350
- GenomeReviews:   CP000736_GR
- KEGG:   sah:SaurJH1_0937
- eggNOG:   COG2070
- GeneTree:   EBGT00050000024707
- HOGENOM:   HBG683237
- OMA:   EFIEKHE
- ProtClustDB:   CLSK885016
- BioCyc:   SAUR359787:SAURJH1_0937-MONOMER
- InterPro:   IPR004136
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF03060 NPD

EC number: =1.13.12.16

Molecular weight: Translated: 38635; Mature: 38635

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: ACT_SITE 182-182 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIGAGYFNTQQLEDEIDYV
CCCHHHHHHHHHCCCCHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKIIEEQQFKCHIDT
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
IIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDEAIANEKAGMDAIVAQGSEAG
HHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCHHHHHCCCCCC
GHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGGIMDGRGVLASIVLGAEGVQMGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCHHHHH
AFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKLARGINNRFIEEMSQYEGDIPDYPI
HEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QNELTSSIRKAAANIGDKELTHMWSGQSPRLATTHPANTIMSNIINQINQIMQYK
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIGAGYFNTQQLEDEIDYV
CCCHHHHHHHHHCCCCHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKIIEEQQFKCHIDT
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
IIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDEAIANEKAGMDAIVAQGSEAG
HHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCHHHHHCCCCCC
GHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGGIMDGRGVLASIVLGAEGVQMGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCHHHHH
AFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKLARGINNRFIEEMSQYEGDIPDYPI
HEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QNELTSSIRKAAANIGDKELTHMWSGQSPRLATTHPANTIMSNIINQINQIMQYK
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA