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The map label for this gene is graS

Identifier: 148267119

GI number: 148267119

Start: 749437

End: 750477

Strand: Direct

Name: graS

Synonym: SaurJH9_0683

Alternate gene names: 148267119

Gene position: 749437-750477 (Clockwise)

Preceding gene: 148267118

Following gene: 148267120

Centisome position: 25.78

GC content: 30.74

Gene sequence:

>1041_bases
ATGAATAATTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGGATATTGTTTTTAAACTTGCT
TATGTTAGGCATTAGTCTAATCGATTATGATTTTCCAATAGACAGTTTATTTTATATTGTTTCTTTGAATTTAAGTTTAA
CAATGATTTTTCTTATATTGACATATTTTAAAGAAGTAAAATTATATAAGCATTTTGACAAAGATAAAGAAATAGAAGAA
ATTAAACATAAAGATTTAGCGGAAACGCCATTTCAACGTCATACAGTTGATTATTTATATCGTCAAATCTCAGCGCACAA
AGAAAAGGTTGTTGAGCAACAGTTACAATTGAACATGCATGAACAAACCATTACAGAATTTGTGCACGACATAAAAACAC
CTGTGACAGCCATGAAATTATTAATTGATCAAGAAAAAAATCAAGAAAGAAAACAGGCATTACTATATGAATGGTCTCGT
ATAAACTCGATGCTGGATACACAGCTGTATATTACTAGATTAGAATCTCAACGCAAAGATATGTATTTTGATTACGTGTC
ACTTAAACGCATGGTCATTGATGAAATACAATTAACAAGACATATTAGTCAGGTTAAAGGTATTGGTTTTGATGTTGACT
TTAAAGTGGATGATTATGTTTATACAGATACAAAATGGTGTCGTATGATTATTAGACAGATTTTGTCAAACGCATTGAAA
TATAGTGAGAATTTTAATATTGAAATTGGGACAGAATTAAATGATCAACATGTTTCGTTATATATTAAAGACTATGGCAG
AGGTATTAGTAAAAAAGATATGCCGCGAATATTTGAACGAGGATTTACGTCAACGGCTAACAGAAATGAAACGACGTCTT
CAGGTATGGGTCTATATTTAGTAAATAGTGTAAAGGATCAATTAGGTATTCACCTGCAAGTCACGTCGACTGTTGGTAAG
GGGACAACTGTCAGATTGATTTTCCCATTACAAAATGAAATTGTTGAACGCATGTCGGAAGTGACAAATTTGTCATTTTA
A

Upstream 100 bases:

>100_bases
AATACGTTAACAGTAAATGTGAATCGTTTACGAAAAAAATTATCTGAAATTAGTATGGATAGTGCAATCGAAACAAAAGT
AGGAAAAGGATATATGGCTC

Downstream 100 bases:

>100_bases
ACATGCGTTTTGTTACTTAGAATTGATACATCAATGCAGCTTCAACGTTATAATAAGATAGATGTTAGTCATATGTTAAA
TTGAAGATACAAGTGCCAAA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Glycopeptide resistance-associated protein S

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEE
IKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALK
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGK
GTTVRLIFPLQNEIVERMSEVTNLSF

Sequences:

>Translated_346_residues
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEE
IKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALK
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGK
GTTVRLIFPLQNEIVERMSEVTNLSF
>Mature_346_residues
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEE
IKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALK
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGK
GTTVRLIFPLQNEIVERMSEVTNLSF

Specific function: Member of the two-component regulatory system graR/graS involved in resistance against cationic antimicrobial peptides (CAMPs). GraS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phospha

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=24.2553191489362, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=20.5357142857143, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRAS_STAA1 (A7WZC5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441247.1
- ProteinModelPortal:   A7WZC5
- SMR:   A7WZC5
- STRING:   A7WZC5
- EnsemblBacteria:   EBSTAT00000004567
- GeneID:   5559294
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0657
- eggNOG:   COG0642
- GeneTree:   EBGT00050000023863
- HOGENOM:   HBG305212
- OMA:   HLAQNAR
- ProtClustDB:   CLSK884800
- BioCyc:   SAUR418127:SAHV_0657-MONOMER
- GO:   GO:0009405
- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 41033; Mature: 41033

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe3d1d00)-; HASH(0xdc913a4)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMH
HHHHHHHHHHHCCCCCHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
EQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHH
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYL
CCCCCCEEECCCCCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEE
VNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF
HHHHHHHHCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMH
HHHHHHHHHHHCCCCCHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
EQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHH
YSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYL
CCCCCCEEECCCCCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEE
VNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF
HHHHHHHHCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA