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The map label for this gene is graR

Identifier: 148267118

GI number: 148267118

Start: 748770

End: 749444

Strand: Direct

Name: graR

Synonym: SaurJH9_0682

Alternate gene names: 148267118

Gene position: 748770-749444 (Clockwise)

Preceding gene: 148267117

Following gene: 148267119

Centisome position: 25.76

GC content: 30.67

Gene sequence:

>675_bases
ATGCAAATACTACTAGTAGAAGATGACAATACTTTGTTTCAAGAATTGAAAAAAGAATTAGAACAATGGGATTTTAATGT
TGCTGGTATTGAAGATTTCGGCAAAGTAATGGATACATTTGAAAGTTTTAATCCTGAAATTGTTATATTGGATGTTCAAT
TACCTAAATATGATGGGTTTTATTGGTGCAGAAAAATGAGAGAAGTTTCCAACGTACCAATATTATTTTTATCATCTCGT
GATAATCCAATGGATCAAGTGATGAGTATGGAACTTGGCGCAGATGATTATATGCAAAAACCTTTCTATACCAATGTATT
AATTGCTAAATTACAAGCGATTTATCGTCGTGTCTATGAGTTTACAGCTGAAGAAAAACGTACATTGACTTGGCAAGATG
CTGTCGTTGATCTATCAAAAGATAGTATACAAAAAGGTGACGATACGATTTTTCTATCCAAAACAGAAATGATTATATTA
GAAATTCTTATTACCAAAAAAAATCAAATCGTTTCGAGAGATACAATTATCACTGCATTATGGGATGATGAAGCATTTGT
TAGTGATAATACGTTAACAGTAAATGTGAATCGTTTACGAAAAAAATTATCTGAAATTAGTATGGATAGTGCAATCGAAA
CAAAAGTAGGAAAAGGATATATGGCTCATGAATAA

Upstream 100 bases:

>100_bases
GATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTA
AATGATATTGGGTGATATGG

Downstream 100 bases:

>100_bases
TTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGGATATTGTTTTTAAACTTGCTTATGTTAG
GCATTAGTCTAATCGATTAT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: Glycopeptide resistance-associated protein R

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSR
DNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

Sequences:

>Translated_224_residues
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSR
DNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE
>Mature_224_residues
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSR
DNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

Specific function: Member of the two-component regulatory system graR/graS involved in resistance against cationic antimicrobial peptides (CAMPs)

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=28.3783783783784, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.981981981982, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=30.6306306306306, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786911, Length=219, Percent_Identity=28.310502283105, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=28.3842794759825, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=32.15859030837, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=27.5109170305677, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=29.2035398230088, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=29.2035398230088, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=26.3636363636364, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=24.1071428571429, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRAR_STAA1 (A7WZC3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441246.1
- ProteinModelPortal:   A7WZC3
- SMR:   A7WZC3
- STRING:   A7WZC3
- EnsemblBacteria:   EBSTAT00000002964
- GeneID:   5560564
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0656
- eggNOG:   COG0745
- GeneTree:   EBGT00050000024053
- HOGENOM:   HBG753323
- OMA:   QNQIVTR
- ProtClustDB:   CLSK884799
- BioCyc:   SAUR418127:SAHV_0656-MONOMER
- GO:   GO:0005737
- GO:   GO:0009405
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 26066; Mature: 26066

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGF
CEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
YWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYE
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
FTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIILEILITKKNQIVSRDTIITAL
HHHHHCCEEEHHHHHEECCHHHHCCCCCEEEEECHHEEEEEEEHHCCCCEEECCEEEEEE
WDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE
ECCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGF
CEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
YWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYE
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
FTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIILEILITKKNQIVSRDTIITAL
HHHHHCCEEEHHHHHEECCHHHHCCCCCEEEEECHHEEEEEEEHHCCCCEEECCEEEEEE
WDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE
ECCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA