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The map label for this gene is brnQ [H]

Identifier: 148266611

GI number: 148266611

Start: 204432

End: 205781

Strand: Reverse

Name: brnQ [H]

Synonym: SaurJH9_0171

Alternate gene names: 148266611

Gene position: 205781-204432 (Counterclockwise)

Preceding gene: 148266612

Following gene: 148266610

Centisome position: 7.08

GC content: 33.04

Gene sequence:

>1350_bases
ATGAAGAAGAAACTAACATTTAAAGAAAACATGTTTATAGGTTCTATGTTATTTGGTTTATTCTTTGGTGCCGGCAATCT
TATCTTCCCAATACACTTGGGTCAAGCTGCTGGTTCTAACGTTTTTATCGCTAACTTAGGATTTTTAATTACAGCAATTG
GCTTACCATTTCTAGGTATCATTGCTATTGGCATTTCAAAGACATCTGGTTTATTTGAAATTGCATCGCGTGTTAATAAA
ACATATGCTTACATTTTCACGATTGCCTTATATCTAGTTATCGGACCATTTTTCGCCTTACCTAGACTGGCAACGACATC
ATTTGAAATTGCATTTTCGCCATTTTTATCACCAAAGCAAATCACTTTATATTTATTTATTTTTAGCTTCGTCTTCTTTG
TGATTGCATGGTTTTTTGCGAGAAAGCCATCAAGAATTTTAGAATATATCGGTAAATTTTTAAATCCGGTATTCTTAGTA
TTATTAGCAATTATTTTATTATTTGCTTTTATCCATCCATTAGGTGGCATATCTGATGCACCTATTAGTAAACAATATCA
ATCACATGCCTTATTTAACGGCTTTTTAGATGGATACAATACCTTAGATGCGCTAGCGTCATTGGCATTTGGTATCATCA
TTGTTGCAACGATTAAAAAGTTAGGTATCGAAAATCCAACTGATATCGCTAAAGAAACAATTAAGTCTGGTACTATCAGT
ATCATTATGATGGGGATTATTTATACCCTACTAGCAATCATGGGTACATTAAGTATTGGTCATTTCAAACTTAGTGAAAA
TGGTGGTATTGCCTTAGCGCAAATTACGCAATACTACTTAGGTAACTACGGTATCGTCCTGTTGTCACTTATCGTTATGG
TTGCTTGTTTAAAAACAGCCATCGGTTTGATTACGGCATTTTCAGAAACATTCGAACACCTTTTCCCTAAAATGAATTAC
CTAGCGATTGCAACAGTTGTAAGCTTTATTTCGTTCTTATTCGCGAATGTTGGCTTAACTAAGATTATTATGTACTCAGT
CCCAGTGTTAATGTTCTTATATCCATTAGCAATTGCCTTGATTGTACTAACATTATTTAGTAGCAAATTCCATCATTCAA
AAATTATTTATCAATGTACCATTTTCTTTACAATGATTGCTGCATTAGTAGATGGATTAAAAGCTAGTCCAGAGTTCATT
TCAAGCACATCATTCTCACAAACTTTGATTAATTTCAGCCAAAAATATTTACCATTATCAGACATTGGTATGGGCTGGGT
TGTTCTCAGTTTGATTGGTTTCATTATCGGCTTCATTATTTATAAAATTAAGCATCGTAAAATTCCATAA

Upstream 100 bases:

>100_bases
GTATTTATTACAAAATTATGTATCACTTTAGGTTTAATTTTTGATATTATCTTAATTAAGTAGATTTTTATAAGTTCTAA
AAAGGAGAACAAATACATAT

Downstream 100 bases:

>100_bases
GCATAATACTATGCCACAGTCATATGTTAAACATATGCTTGTGGCATTTTTTATTCATACTACATTAAACTGCAATCGTA
TACATACATATCACTGATTA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNK
TYAYIFTIALYLVIGPFFALPRLATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPTDIAKETIKSGTIS
IIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNY
LAIATVVSFISFLFANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDGLKASPEFI
SSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIP

Sequences:

>Translated_449_residues
MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNK
TYAYIFTIALYLVIGPFFALPRLATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPTDIAKETIKSGTIS
IIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNY
LAIATVVSFISFLFANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDGLKASPEFI
SSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIP
>Mature_449_residues
MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNK
TYAYIFTIALYLVIGPFFALPRLATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPTDIAKETIKSGTIS
IIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNY
LAIATVVSFISFLFANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDGLKASPEFI
SSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIP

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=448, Percent_Identity=33.2589285714286, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49502; Mature: 49502

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAIGLPFLGI
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHH
IAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPRLATTSFEIAFSPFLSPKQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHH
ITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLVLLAIILLFAFIHPLGGISDA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPTDIAKETIKSGTIS
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHH
IIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGIVLLSLIVMVACLKTA
HHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IGLITAFSETFEHLFPKMNYLAIATVVSFISFLFANVGLTKIIMYSVPVLMFLYPLAIAL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IVLTLFSSKFHHSKIIYQCTIFFTMIAALVDGLKASPEFISSTSFSQTLINFSQKYLPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCHH
DIGMGWVVLSLIGFIIGFIIYKIKHRKIP
HCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAIGLPFLGI
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHH
IAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPRLATTSFEIAFSPFLSPKQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHH
ITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLVLLAIILLFAFIHPLGGISDA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPTDIAKETIKSGTIS
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHH
IIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGIVLLSLIVMVACLKTA
HHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IGLITAFSETFEHLFPKMNYLAIATVVSFISFLFANVGLTKIIMYSVPVLMFLYPLAIAL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IVLTLFSSKFHHSKIIYQCTIFFTMIAALVDGLKASPEFISSTSFSQTLINFSQKYLPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCHH
DIGMGWVVLSLIGFIIGFIIYKIKHRKIP
HCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8544834 [H]