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The map label for this gene is nasA [H]

Identifier: 148243801

GI number: 148243801

Start: 196416

End: 198830

Strand: Direct

Name: nasA [H]

Synonym: Acry_3284

Alternate gene names: 148243801

Gene position: 196416-198830 (Clockwise)

Preceding gene: 148243800

Following gene: 148243802

Centisome position: 96.48

GC content: 70.19

Gene sequence:

>2415_bases
ATGCATTTTTGCTCTGGACCGACTACGCCGCCAATCTCGCCGCCTGATCGATCGCGTCTCGGTCAAATCACACCTCGTTC
GATCAACCTGACAATCCCGTCCGGACAGTTCCTGACCGAGGATTACTACGTGGCCAACAAGCTGATGAAGGGGTTCATCG
GCAGTGCCAATATTGACACCAATTCCCGGCTTTGTATGGCGTCCGCGGTGGCCGCCCACCGCCGGTGCTTCGGCGAGGAC
GTCGTCCCGGGGATCTACGAGGATTTCGACGAGGCGGAACTGATCATCTTCGCCGGCTCGAACGCCGCCTGGTGCCATCC
GGTCCTGTTCCAGCGCGCACTTGCCGCCCGGGCGACGCGCGGGACGCGGATTGTCGTGATCGATCCCCGCCGTAGCGCGA
CCGCCGAGTTCGCCGACCTGCACCTCGCCGTGCGCCCCGGCGGCGACCTCGCCCTGTTCTGCGCGCTGCTCGCCGCCTGC
GCGGACCGCGGCGCGCTCGATCGCGACTATATCGCGCGGCACACCGCCGGGTTCGACGCCGCGCTGGCCGCCGCGCGGCA
GGCCGAGCACGGGATCGATCCCGCCGCATTCGAAACCCTCGCCGGCTGGGTCGCCTCCACCCCGCGCATGGTGACCGCCT
TTTCGCAGGGCGTGAACCAGTCCGAGACGGCGACCGACGCAATCGGCGCGATCCTGAACCTCCACCTCGCCACCGGACGG
ATCGGCCGGCCCGGCGCCGGACCGTTCTCGCTGACCGGCCAGCCCAACGCGATGGGCGGCCGCGAGGCGGGCGGCCTCGC
CAACCAGCTCGCCGCGCATCTCGGCTTCGACGACCCCGACGACCACGCGACGCTCTCCGAATTCTGGGGCGCCCCCAACC
TCGTGCGCCGGCCCGGGCTGAAGGCGGTCGACCTGTTCCGTGCCGTCGCCGACGGCAAGATACGCGCCCTCTGGATCGCC
GGCACCAACCCCGCCGAATCCCTGCCAGCGAGCCTCGACGTGCGCGACGCGCTATCCCGTTGTCCGCACCTCGTGGTCGC
CGATTGCTGGCCCACGGCGACGACCGCCCATGCCCATGTCCTGCTGCCAGCGGCAGGGTGGTCCGAGAAGGACGGCACCG
TCACCAATTCAGAGCGCATGATCTCCCGCCAGCGGGCGTTTCGTCCCGCTCCCGGTGCTGCTCGACCCGACTGGTGGATG
TTCGCCGAACTCGCCCGGCGGCTCGGCCATGGCGCGGCCTTCGCCTGGGACGGGCCGGCCGCGATCTTCCGCGAACACGC
GGCACTCTCCGCTTTCCGCAACTTCGGGCGACGGCGGTTCAATATCGGCGGGCTCGCTGCCATCCCGGATGACGATTACG
ACGCAATGCGCCCGCTGCGCTGGCCTCTGGCCGGAGATCGCCTCTTTGCCGAGGGAAACTTCCCGACGCCAGACGGGCGG
GCCCGCTTCGTCGCCATATCACCGCTCCGCCACGCGCGCACGGATTTCACCCTCAATACCGGCCGGCTGCGCGACCAATG
GCACACCATGACGCGCACCGGTCGCGTCGCCGAACTGATGGCCCACCAAGATGCCGCGACCCTGGAACTCGCACCGACGG
ACGCGGCGCGACTCGGCGTGCTGCCCGGTGATCTTGTCGCCGCCGGGAGCGAAGGGGCGCGCATCGTCTTCCCGGCAGCG
ATCTCGCCGGGACAGTGCGAAGGTTCGGTGTTCGCTGCGATACACTGGACCGCAGCGCAGGGCAGCGCCGGAAGCGTCAA
CCGCCTCACTGTTTCAGCGACCGACCCGATCTCCGGCCAGCCGGCCTTCAAGCATTCGCCAGTGAAACTTGCGGCGGTGC
CGACGCTCTGGCGGGCCATTGCTATCGGACGTGCCGCTTCCTGCGCGATGACGGCTGGCAAACAAGCCGTCTGGTCGCAG
GCACTACGCCCGGGCGGGCTGGCGCTGTCGCGCTTTACCGGGTTTGCCCCGCTCGCCGCCCGCAGCGCCGTGGACGCGGC
ACGGTGGCTGCTGGTGCTGCCGGAGGGTGCCGAATTCCTCGACTACGCCGACCCGGCGCGCGGGGTCTTCCGCCTCGCCG
TGCTCGACCGGGCCGGCGTCCAGCTCCTTCTCCACGTCGCGCCGGCGGAGCTTGCGCTGCCGGACCCCGAGGCGCTGGAG
GCGCTGTTCGCGGCCCCACAAGCAGGAGCCGATCCGACGCTCCTGCTTGGCCAGCCCGCCCGCGCCACCGCGACCCGCGG
GCGGCAGGTCTGCGTCTGCAACGCGGTTGCCGAACCCGCGATCCGTGCCGCGATCCGCGCCGGCTGCCGGAGCGTCGAGG
CGATCGGCGCGGCCACCGGCGCCGGCACCGGCTGCGGTTCATGCCACTCCGAACTGAAGGGATTTCTCCGCCATGACCTC
GTTGCCGCCGAATAG

Upstream 100 bases:

>100_bases
CATGCCCAGAGATTTCTCGCCACCCACGACCAGATCACCACCCTGTTCCGCCCAAAACGCCATCGCCTCTCCGCCAGATC
CTACCGCCACGCAAGAGCCG

Downstream 100 bases:

>100_bases
CAAGGCGCATCCCGTCATCCTCGCCGGCACCGGGCCGGGCGATCCGGATCTTCTCACCCTGCGGGCCGTACGGGCGATCG
GCGCCGCCGAAATCATCCTG

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MHFCSGPTTPPISPPDRSRLGQITPRSINLTIPSGQFLTEDYYVANKLMKGFIGSANIDTNSRLCMASAVAAHRRCFGED
VVPGIYEDFDEAELIIFAGSNAAWCHPVLFQRALAARATRGTRIVVIDPRRSATAEFADLHLAVRPGGDLALFCALLAAC
ADRGALDRDYIARHTAGFDAALAAARQAEHGIDPAAFETLAGWVASTPRMVTAFSQGVNQSETATDAIGAILNLHLATGR
IGRPGAGPFSLTGQPNAMGGREAGGLANQLAAHLGFDDPDDHATLSEFWGAPNLVRRPGLKAVDLFRAVADGKIRALWIA
GTNPAESLPASLDVRDALSRCPHLVVADCWPTATTAHAHVLLPAAGWSEKDGTVTNSERMISRQRAFRPAPGAARPDWWM
FAELARRLGHGAAFAWDGPAAIFREHAALSAFRNFGRRRFNIGGLAAIPDDDYDAMRPLRWPLAGDRLFAEGNFPTPDGR
ARFVAISPLRHARTDFTLNTGRLRDQWHTMTRTGRVAELMAHQDAATLELAPTDAARLGVLPGDLVAAGSEGARIVFPAA
ISPGQCEGSVFAAIHWTAAQGSAGSVNRLTVSATDPISGQPAFKHSPVKLAAVPTLWRAIAIGRAASCAMTAGKQAVWSQ
ALRPGGLALSRFTGFAPLAARSAVDAARWLLVLPEGAEFLDYADPARGVFRLAVLDRAGVQLLLHVAPAELALPDPEALE
ALFAAPQAGADPTLLLGQPARATATRGRQVCVCNAVAEPAIRAAIRAGCRSVEAIGAATGAGTGCGSCHSELKGFLRHDL
VAAE

Sequences:

>Translated_804_residues
MHFCSGPTTPPISPPDRSRLGQITPRSINLTIPSGQFLTEDYYVANKLMKGFIGSANIDTNSRLCMASAVAAHRRCFGED
VVPGIYEDFDEAELIIFAGSNAAWCHPVLFQRALAARATRGTRIVVIDPRRSATAEFADLHLAVRPGGDLALFCALLAAC
ADRGALDRDYIARHTAGFDAALAAARQAEHGIDPAAFETLAGWVASTPRMVTAFSQGVNQSETATDAIGAILNLHLATGR
IGRPGAGPFSLTGQPNAMGGREAGGLANQLAAHLGFDDPDDHATLSEFWGAPNLVRRPGLKAVDLFRAVADGKIRALWIA
GTNPAESLPASLDVRDALSRCPHLVVADCWPTATTAHAHVLLPAAGWSEKDGTVTNSERMISRQRAFRPAPGAARPDWWM
FAELARRLGHGAAFAWDGPAAIFREHAALSAFRNFGRRRFNIGGLAAIPDDDYDAMRPLRWPLAGDRLFAEGNFPTPDGR
ARFVAISPLRHARTDFTLNTGRLRDQWHTMTRTGRVAELMAHQDAATLELAPTDAARLGVLPGDLVAAGSEGARIVFPAA
ISPGQCEGSVFAAIHWTAAQGSAGSVNRLTVSATDPISGQPAFKHSPVKLAAVPTLWRAIAIGRAASCAMTAGKQAVWSQ
ALRPGGLALSRFTGFAPLAARSAVDAARWLLVLPEGAEFLDYADPARGVFRLAVLDRAGVQLLLHVAPAELALPDPEALE
ALFAAPQAGADPTLLLGQPARATATRGRQVCVCNAVAEPAIRAAIRAGCRSVEAIGAATGAGTGCGSCHSELKGFLRHDL
VAAE
>Mature_804_residues
MHFCSGPTTPPISPPDRSRLGQITPRSINLTIPSGQFLTEDYYVANKLMKGFIGSANIDTNSRLCMASAVAAHRRCFGED
VVPGIYEDFDEAELIIFAGSNAAWCHPVLFQRALAARATRGTRIVVIDPRRSATAEFADLHLAVRPGGDLALFCALLAAC
ADRGALDRDYIARHTAGFDAALAAARQAEHGIDPAAFETLAGWVASTPRMVTAFSQGVNQSETATDAIGAILNLHLATGR
IGRPGAGPFSLTGQPNAMGGREAGGLANQLAAHLGFDDPDDHATLSEFWGAPNLVRRPGLKAVDLFRAVADGKIRALWIA
GTNPAESLPASLDVRDALSRCPHLVVADCWPTATTAHAHVLLPAAGWSEKDGTVTNSERMISRQRAFRPAPGAARPDWWM
FAELARRLGHGAAFAWDGPAAIFREHAALSAFRNFGRRRFNIGGLAAIPDDDYDAMRPLRWPLAGDRLFAEGNFPTPDGR
ARFVAISPLRHARTDFTLNTGRLRDQWHTMTRTGRVAELMAHQDAATLELAPTDAARLGVLPGDLVAAGSEGARIVFPAA
ISPGQCEGSVFAAIHWTAAQGSAGSVNRLTVSATDPISGQPAFKHSPVKLAAVPTLWRAIAIGRAASCAMTAGKQAVWSQ
ALRPGGLALSRFTGFAPLAARSAVDAARWLLVLPEGAEFLDYADPARGVFRLAVLDRAGVQLLLHVAPAELALPDPEALE
ALFAAPQAGADPTLLLGQPARATATRGRQVCVCNAVAEPAIRAAIRAGCRSVEAIGAATGAGTGCGSCHSELKGFLRHDL
VAAE

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=706, Percent_Identity=27.6203966005666, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI3868721, Length=615, Percent_Identity=25.2032520325203, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1787778, Length=621, Percent_Identity=24.1545893719807, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI3868719, Length=466, Percent_Identity=21.4592274678112, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI3868720, Length=160, Percent_Identity=29.375, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87081994, Length=511, Percent_Identity=21.9178082191781, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 84884; Mature: 84884

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFCSGPTTPPISPPDRSRLGQITPRSINLTIPSGQFLTEDYYVANKLMKGFIGSANIDT
CCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
NSRLCMASAVAAHRRCFGEDVVPGIYEDFDEAELIIFAGSNAAWCHPVLFQRALAARATR
CCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
GTRIVVIDPRRSATAEFADLHLAVRPGGDLALFCALLAACADRGALDRDYIARHTAGFDA
CCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHH
ALAAARQAEHGIDPAAFETLAGWVASTPRMVTAFSQGVNQSETATDAIGAILNLHLATGR
HHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
IGRPGAGPFSLTGQPNAMGGREAGGLANQLAAHLGFDDPDDHATLSEFWGAPNLVRRPGL
CCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHCCCC
KAVDLFRAVADGKIRALWIAGTNPAESLPASLDVRDALSRCPHLVVADCWPTATTAHAHV
HHHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEE
LLPAAGWSEKDGTVTNSERMISRQRAFRPAPGAARPDWWMFAELARRLGHGAAFAWDGPA
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHH
AIFREHAALSAFRNFGRRRFNIGGLAAIPDDDYDAMRPLRWPLAGDRLFAEGNFPTPDGR
HHHHHHHHHHHHHHCCCEEECCCCEEECCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCC
ARFVAISPLRHARTDFTLNTGRLRDQWHTMTRTGRVAELMAHQDAATLELAPTDAARLGV
EEEEEECCHHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCHHHCCC
LPGDLVAAGSEGARIVFPAAISPGQCEGSVFAAIHWTAAQGSAGSVNRLTVSATDPISGQ
CCCHHEECCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCC
PAFKHSPVKLAAVPTLWRAIAIGRAASCAMTAGKQAVWSQALRPGGLALSRFTGFAPLAA
CCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCCEEHHHHCCCCHHHH
RSAVDAARWLLVLPEGAEFLDYADPARGVFRLAVLDRAGVQLLLHVAPAELALPDPEALE
HHHHHHEEEEEECCCCCHHHCCCCCHHHHEEHHHHHHCCCEEEEEECCHHHCCCCHHHHH
ALFAAPQAGADPTLLLGQPARATATRGRQVCVCNAVAEPAIRAAIRAGCRSVEAIGAATG
HHHHCCCCCCCCEEEECCCCHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
AGTGCGSCHSELKGFLRHDLVAAE
CCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFCSGPTTPPISPPDRSRLGQITPRSINLTIPSGQFLTEDYYVANKLMKGFIGSANIDT
CCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
NSRLCMASAVAAHRRCFGEDVVPGIYEDFDEAELIIFAGSNAAWCHPVLFQRALAARATR
CCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
GTRIVVIDPRRSATAEFADLHLAVRPGGDLALFCALLAACADRGALDRDYIARHTAGFDA
CCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHH
ALAAARQAEHGIDPAAFETLAGWVASTPRMVTAFSQGVNQSETATDAIGAILNLHLATGR
HHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
IGRPGAGPFSLTGQPNAMGGREAGGLANQLAAHLGFDDPDDHATLSEFWGAPNLVRRPGL
CCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHCCCC
KAVDLFRAVADGKIRALWIAGTNPAESLPASLDVRDALSRCPHLVVADCWPTATTAHAHV
HHHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEE
LLPAAGWSEKDGTVTNSERMISRQRAFRPAPGAARPDWWMFAELARRLGHGAAFAWDGPA
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHH
AIFREHAALSAFRNFGRRRFNIGGLAAIPDDDYDAMRPLRWPLAGDRLFAEGNFPTPDGR
HHHHHHHHHHHHHHCCCEEECCCCEEECCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCC
ARFVAISPLRHARTDFTLNTGRLRDQWHTMTRTGRVAELMAHQDAATLELAPTDAARLGV
EEEEEECCHHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCHHHCCC
LPGDLVAAGSEGARIVFPAAISPGQCEGSVFAAIHWTAAQGSAGSVNRLTVSATDPISGQ
CCCHHEECCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCC
PAFKHSPVKLAAVPTLWRAIAIGRAASCAMTAGKQAVWSQALRPGGLALSRFTGFAPLAA
CCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCCEEHHHHCCCCHHHH
RSAVDAARWLLVLPEGAEFLDYADPARGVFRLAVLDRAGVQLLLHVAPAELALPDPEALE
HHHHHHEEEEEECCCCCHHHCCCCCHHHHEEHHHHHHCCCEEEEEECCHHHCCCCHHHHH
ALFAAPQAGADPTLLLGQPARATATRGRQVCVCNAVAEPAIRAAIRAGCRSVEAIGAATG
HHHHCCCCCCCCEEEECCCCHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
AGTGCGSCHSELKGFLRHDLVAAE
CCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]