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The map label for this gene is nhaA3

Identifier: 148243738

GI number: 148243738

Start: 127229

End: 128599

Strand: Direct

Name: nhaA3

Synonym: Acry_3215

Alternate gene names: 148243738

Gene position: 127229-128599 (Clockwise)

Preceding gene: 148243736

Following gene: 148243739

Centisome position: 62.49

GC content: 60.98

Gene sequence:

>1371_bases
TTGATTACGGACCAGAACAGTAAGCCGCAGCAAGGCGAACTGCCGACCACACACATCGACGCGCTGACCAACTCGTTTTC
GCAGTTCCTGCGCATCGAAGCCACGAGTGGTGCCGTTCTCCTGTTGGCCACCGTCGTTGCATTGACGCTTTCCAACTCAC
CCTGGTCCGATGGCTTCAGATCTCTTTGGGAGACACCAATCGGCATTCAGATCGGTGCGTTCCAGTTTCTGCGCTCTTTG
CGGGATCTGATCAACGACGGTCTAATGACGCTGTTCTTCTTCATCGTCGCGCTCGAGATCAAGCGCGAAGTGGTGCTTGG
TGAACTTCGGAACCCGCGCATGGTGGCTCTCTCGGTCGTAGCTGCGGCAGGAGGCATGCTTGTGCCCATGGGCTTGTATC
TGGCGCTGCAGCATGGCCAACCTGGGCAGCATGGTTGGGGCGTCGTGATGCCGACCGACACAGCTTTCGTCATCGGCTGC
CTGGCGCTCCTTGGTTCGCGTGTGCCTCCGGGCCTACGCGTCTTCCTGCTATCGCTTGCGGTCGTCGACGACCTTGCCGC
CATCCTCGTGGTCGCCGTCGGTTACAGTCGATCAATCGACTGGACTGCCCTTGCGCTCGGCGCCGTCGGACTGGTGATAA
TTCGGGGGATGGCATTACTCGGCGTTCGTAACATCCGCGTCTACTTTCTGGCTGGGGCAATAATCTGGCTCGCCGTCAAT
GCGTCCGGCATCCACGCGACGATTGTTGGTGTTATCCTCGGGCTCATGACACCGACGGCTGGCTGGGTGAGCGACCAGCG
TCTGGGGGAAATCTTGCGAAAGGTGCTCTCCTATCCCCCGGGAGATCATTGGAGTGGCGATACCGAGGATAACCGGGCGC
TGCAGGTTGCCGGTATCGCGGTGCGCGAGACGTTGTCGCCCGTCGAGAGGCTCGAGGCCATGCTCCATCCCTGGGTCGCC
TTCGGCGTTATGCCGCTTTTCGCGCTTGCAAACGCGGGGGTACCGATCACGATCAAGGGACTTATCAACCCAGTCTCGCT
GGCGGTGATGGCGGGCTTCGTCCTCGGCAAGCCGATCGGCGTCACAGCATTCGCATGGCTGGGGGTGCGCACGGGTGTCG
CCATTCGGCCGGCGGGGCTCACCTGGGGGGGACTTGTCGGTGGGGCTCTTCTTACCGGTATCGGATTCACCATGGCGCTG
TTTATCGCCGGCCAGGCATTTCAAGATGCCACGCTCAACGCAGCGAAGCTGGGTATCCTTGCTGCCTCAGTCGTCTCTTC
TGTTGCTGGTCTCACGCTGCTGTGCGCGTTGCCTCGGAGTGACGGAACGCCTATGTTTCGGGGGCGCAGAGGCGGAGGAG
AACACCCATGA

Upstream 100 bases:

>100_bases
TATGCAGTCGGCGGGAAAAATTGGGCTTGCCGGGACGGGTAACCGCCTCGACACCCCGGGCGGGGTGGGTTTTCTCGAGA
TATAAGGGGAGATGGCCGAT

Downstream 100 bases:

>100_bases
CATACCCCGCTCTGGCCCATCGCGGCATGAAAAAAAGTCTCCCAAGAAGCTAGTTGGCCCTGACTCCTTTACACATCGAT
AACCGGGTCAGTTGCGTTAT

Product: Na+/H+ antiporter NhaA

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA 3

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MITDQNSKPQQGELPTTHIDALTNSFSQFLRIEATSGAVLLLATVVALTLSNSPWSDGFRSLWETPIGIQIGAFQFLRSL
RDLINDGLMTLFFFIVALEIKREVVLGELRNPRMVALSVVAAAGGMLVPMGLYLALQHGQPGQHGWGVVMPTDTAFVIGC
LALLGSRVPPGLRVFLLSLAVVDDLAAILVVAVGYSRSIDWTALALGAVGLVIIRGMALLGVRNIRVYFLAGAIIWLAVN
ASGIHATIVGVILGLMTPTAGWVSDQRLGEILRKVLSYPPGDHWSGDTEDNRALQVAGIAVRETLSPVERLEAMLHPWVA
FGVMPLFALANAGVPITIKGLINPVSLAVMAGFVLGKPIGVTAFAWLGVRTGVAIRPAGLTWGGLVGGALLTGIGFTMAL
FIAGQAFQDATLNAAKLGILAASVVSSVAGLTLLCALPRSDGTPMFRGRRGGGEHP

Sequences:

>Translated_456_residues
MITDQNSKPQQGELPTTHIDALTNSFSQFLRIEATSGAVLLLATVVALTLSNSPWSDGFRSLWETPIGIQIGAFQFLRSL
RDLINDGLMTLFFFIVALEIKREVVLGELRNPRMVALSVVAAAGGMLVPMGLYLALQHGQPGQHGWGVVMPTDTAFVIGC
LALLGSRVPPGLRVFLLSLAVVDDLAAILVVAVGYSRSIDWTALALGAVGLVIIRGMALLGVRNIRVYFLAGAIIWLAVN
ASGIHATIVGVILGLMTPTAGWVSDQRLGEILRKVLSYPPGDHWSGDTEDNRALQVAGIAVRETLSPVERLEAMLHPWVA
FGVMPLFALANAGVPITIKGLINPVSLAVMAGFVLGKPIGVTAFAWLGVRTGVAIRPAGLTWGGLVGGALLTGIGFTMAL
FIAGQAFQDATLNAAKLGILAASVVSSVAGLTLLCALPRSDGTPMFRGRRGGGEHP
>Mature_456_residues
MITDQNSKPQQGELPTTHIDALTNSFSQFLRIEATSGAVLLLATVVALTLSNSPWSDGFRSLWETPIGIQIGAFQFLRSL
RDLINDGLMTLFFFIVALEIKREVVLGELRNPRMVALSVVAAAGGMLVPMGLYLALQHGQPGQHGWGVVMPTDTAFVIGC
LALLGSRVPPGLRVFLLSLAVVDDLAAILVVAVGYSRSIDWTALALGAVGLVIIRGMALLGVRNIRVYFLAGAIIWLAVN
ASGIHATIVGVILGLMTPTAGWVSDQRLGEILRKVLSYPPGDHWSGDTEDNRALQVAGIAVRETLSPVERLEAMLHPWVA
FGVMPLFALANAGVPITIKGLINPVSLAVMAGFVLGKPIGVTAFAWLGVRTGVAIRPAGLTWGGLVGGALLTGIGFTMAL
FIAGQAFQDATLNAAKLGILAASVVSSVAGLTLLCALPRSDGTPMFRGRRGGGEHP

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=419, Percent_Identity=37.2315035799523, Blast_Score=251, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA3_ACICJ (A5FTA4)

Other databases:

- EMBL:   CP000689
- RefSeq:   YP_001219978.1
- ProteinModelPortal:   A5FTA4
- SMR:   A5FTA4
- GeneID:   5158932
- GenomeReviews:   CP000689_GR
- KEGG:   acr:Acry_3215
- HOGENOM:   HBG665619
- OMA:   LMTFFFF
- ProtClustDB:   CLSK807773
- BioCyc:   ACRY349163:ACRY_3215-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 47927; Mature: 47927

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe889968)-; HASH(0xea9b2c8)-; HASH(0xdbbd19c)-; HASH(0xf43e82c)-; HASH(0xf5e5638)-; HASH(0xfd43b5c)-; HASH(0xdbbd664)-; HASH(0xf6c85d4)-; HASH(0xf74c3a0)-; HASH(0xaf6a364)-; HASH(0xdbbd16c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDQNSKPQQGELPTTHIDALTNSFSQFLRIEATSGAVLLLATVVALTLSNSPWSDGFR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCHHHH
SLWETPIGIQIGAFQFLRSLRDLINDGLMTLFFFIVALEIKREVVLGELRNPRMVALSVV
HHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AAAGGMLVPMGLYLALQHGQPGQHGWGVVMPTDTAFVIGCLALLGSRVPPGLRVFLLSLA
HHCCCHHHHHHHHHEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
VVDDLAAILVVAVGYSRSIDWTALALGAVGLVIIRGMALLGVRNIRVYFLAGAIIWLAVN
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
ASGIHATIVGVILGLMTPTAGWVSDQRLGEILRKVLSYPPGDHWSGDTEDNRALQVAGIA
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHH
VRETLSPVERLEAMLHPWVAFGVMPLFALANAGVPITIKGLINPVSLAVMAGFVLGKPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHCCCCC
VTAFAWLGVRTGVAIRPAGLTWGGLVGGALLTGIGFTMALFIAGQAFQDATLNAAKLGIL
HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHH
AASVVSSVAGLTLLCALPRSDGTPMFRGRRGGGEHP
HHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MITDQNSKPQQGELPTTHIDALTNSFSQFLRIEATSGAVLLLATVVALTLSNSPWSDGFR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCHHHH
SLWETPIGIQIGAFQFLRSLRDLINDGLMTLFFFIVALEIKREVVLGELRNPRMVALSVV
HHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AAAGGMLVPMGLYLALQHGQPGQHGWGVVMPTDTAFVIGCLALLGSRVPPGLRVFLLSLA
HHCCCHHHHHHHHHEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
VVDDLAAILVVAVGYSRSIDWTALALGAVGLVIIRGMALLGVRNIRVYFLAGAIIWLAVN
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
ASGIHATIVGVILGLMTPTAGWVSDQRLGEILRKVLSYPPGDHWSGDTEDNRALQVAGIA
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHH
VRETLSPVERLEAMLHPWVAFGVMPLFALANAGVPITIKGLINPVSLAVMAGFVLGKPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHCCCCC
VTAFAWLGVRTGVAIRPAGLTWGGLVGGALLTGIGFTMALFIAGQAFQDATLNAAKLGIL
HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHH
AASVVSSVAGLTLLCALPRSDGTPMFRGRRGGGEHP
HHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA