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The map label for this gene is hlyU [H]

Identifier: 147675700

GI number: 147675700

Start: 215990

End: 216247

Strand: Direct

Name: hlyU [H]

Synonym: VC0395_A0210

Alternate gene names: 147675700

Gene position: 215990-216247 (Clockwise)

Preceding gene: 147674750

Following gene: 147674115

Centisome position: 7.14

GC content: 46.12

Gene sequence:

>258_bases
TTGCTCAAAGCGATGGCCAATGAGCGGCGACTGCAAATTTTATGTATGTTACTGGACAATGAACTGTCGGTAGGGGAATT
GAGCAGCCGTTTGGAACTCAGCCAGTCTGCTCTTTCTCAACATCTAGCGTGGCTACGTCGTGATGGTTTAGTGAATACAC
GTAAAGAAGCGCAAACCGTGTTTTATACGTTGAGCAGTACAGAAGTGAAGGCGATGATTGAACTGTTGCACCGTCTTTAT
TGTCAGGCGAATCAGTAG

Upstream 100 bases:

>100_bases
AGAGAAAAGTGAATGAATGGGTTGGAATTAAAATGCCGTATTTAAAGGGGGCACCAATGAATCTCCAAGAGATGGAAAAA
ATTCTGCCAAAGCGGTAGTT

Downstream 100 bases:

>100_bases
GGTGGTCGAAAAAAAGGTATAAAAAAACCGGCGTAGGCCGGTTTTTTGTTGCTGAAGATCAAACTCTATTACAGAGCGTT
GATAGCAGCAAAGAAGCGAG

Product: transcriptional activator HlyU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEAQTVFYTLSSTEVKAMIELLHRLY
CQANQ

Sequences:

>Translated_85_residues
MLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEAQTVFYTLSSTEVKAMIELLHRLY
CQANQ
>Mature_85_residues
MLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEAQTVFYTLSSTEVKAMIELLHRLY
CQANQ

Specific function: Up-regulates the expression of the hemolysin gene, hlyA, and may promote expression of other virulence determinants in vivo. It may have both positive and negative regulator activities [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=82, Percent_Identity=43.9024390243902, Blast_Score=71, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 9780; Mature: 9780

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEAQTV
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FYTLSSTEVKAMIELLHRLYCQANQ
HEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEAQTV
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FYTLSSTEVKAMIELLHRLYCQANQ
HEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8231807; 9457888; 10952301 [H]