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The map label for this gene is guaA [H]

Identifier: 147675501

GI number: 147675501

Start: 312301

End: 313854

Strand: Direct

Name: guaA [H]

Synonym: VC0395_A0297

Alternate gene names: 147675501

Gene position: 312301-313854 (Clockwise)

Preceding gene: 147673264

Following gene: 147674479

Centisome position: 10.33

GC content: 48.78

Gene sequence:

>1554_bases
ATGACTAAAAATATTCATGACCAACGAATTCTGATCCTCGATTTCGGATCTCAATATACCCAGCTAGTAGCCCGTCGCGT
GCGCGAGATCGGTGTGTACTGTGAGCTGTGGAGCTGGGATGTAGAAGAGGCGGACATTCGCGAATTCAATCCTGACGGCA
TTATTCTTTCGGGGGGACCTGAAAGCGTGACCGAAGCTAATTCACCACGTGCCCCGCAATATGTGTTTGACAGTGGTGTG
CCTGTCTTTGGTGTTTGCTACGGCATGCAGACCATGGCTGAGCAGTTAGGAGGCCGTGTTGCGACTTCTGATGAGCGTGA
ATTTGGTTATGCTCAGGTAAAAATTTCTGGCGAATCGGCGCTATTTAAAGATCTCGATCTCACGCAAGACGTTTGGATGA
GCCACGGTGACAAAGTGGTCGAAATCCCAGCGGATTTCGTGAAAATCGGTGAGACCGATACCTGCCCATACGCTGCGATG
GCCAATGAAGAGAAGAAATACTACGGCGTGCAGTTCCACCCCGAAGTGACACACACCAAAAACGGTTTACAGATGCTGGA
GAACTTTGTTCTGGGCGTGTGTGGCTGTGAGCGTCTGTGGACGTCTGAATCCATCATCGAAGATGCGGTTGCTCGCATCA
AAGAACAAGTGGGTAACGATGAAGTGATCCTTGGCCTGTCGGGCGGTGTCGATTCTTCTGTGGTTGCCATGCTGGTTCAC
CGTGCGATTGGAAGTAAGCTCACTTGTGTGTTCGTGGACAACGGACTACTTCGTTTAAACGAAGGCGAGCAAGTGATGGA
GATGTTTGGTGATAAATTTGGCCTCAACATCATTAAAGTCGATGCAGAAGAGCGCTTCCTAAAAGCGCTAGAAGGCATTG
ATGAGCCTGAAGCAAAACGCAAAACCATCGGCCGTGTGTTTGTTGAAGTATTTGATGAAGAATCGAAAAAACTCAGCAAC
GCAAAATGGTTGGCGCAAGGCACCATCTATCCAGATGTGATTGAATCTGCCGCTTCAAAAACCGGTAAAGCGCATGTGAT
CAAATCGCACCACAACGTGGGCGGTTTACCTGATGACATGAAAATGGGCTTGGTTGAGCCACTGCGTGAACTGTTTAAAG
ATGAAGTGCGCAAGATTGGCCTTGAGCTTGGTTTACCTTACAACATGCTTTACCGCCACCCATTCCCAGGCCCAGGCCTT
GGTGTGCGTGTACTGGGTGAAGTGAAGAAAGAGTACTGTGATTTATTGCGTCGTGCAGATGCAATCTTTATTGAAGAGCT
GCACGCAGCGGATCTGTACAACAAAGTTTCTCAAGCCTTTACTGTGTTCTTGCCAGTGCGTTCAGTTGGTGTCATGGGCG
ATGGCCGTAAGTACGATTGGGTTGTGTCATTGCGCGCAGTCGAAACCATCGACTTTATGACCGCACATTGGGCACATCTA
CCTTATGAGTTCTTGGGTAAGGTTTCCAACCGCATTATCAACGAAGTGAATGGCATTTCTCGCGTAGTGTATGACATCTC
AGGTAAGCCACCAGCGACCATTGAGTGGGAATAA

Upstream 100 bases:

>100_bases
ACGTTTGCTTAAATGCTTGATGAGTGAATGTGAGGCGAGTAGACTCGCCTCCGTTGAAGAATCCCCCGTATTAATTCTCG
GCTGATAAGACTGCTAAACA

Downstream 100 bases:

>100_bases
CTACTTAATAATGCGGATGAATATTCCGGATTAATCATATAAAACCTCAGGCATAGCCTGAGGTTTTTTCTTTTACAAGA
GAATCTGTTGTTTTTGTATT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGPESVTEANSPRAPQYVFDSGV
PVFGVCYGMQTMAEQLGGRVATSDEREFGYAQVKISGESALFKDLDLTQDVWMSHGDKVVEIPADFVKIGETDTCPYAAM
ANEEKKYYGVQFHPEVTHTKNGLQMLENFVLGVCGCERLWTSESIIEDAVARIKEQVGNDEVILGLSGGVDSSVVAMLVH
RAIGSKLTCVFVDNGLLRLNEGEQVMEMFGDKFGLNIIKVDAEERFLKALEGIDEPEAKRKTIGRVFVEVFDEESKKLSN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMKMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGL
GVRVLGEVKKEYCDLLRRADAIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYEFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE

Sequences:

>Translated_517_residues
MTKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGPESVTEANSPRAPQYVFDSGV
PVFGVCYGMQTMAEQLGGRVATSDEREFGYAQVKISGESALFKDLDLTQDVWMSHGDKVVEIPADFVKIGETDTCPYAAM
ANEEKKYYGVQFHPEVTHTKNGLQMLENFVLGVCGCERLWTSESIIEDAVARIKEQVGNDEVILGLSGGVDSSVVAMLVH
RAIGSKLTCVFVDNGLLRLNEGEQVMEMFGDKFGLNIIKVDAEERFLKALEGIDEPEAKRKTIGRVFVEVFDEESKKLSN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMKMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGL
GVRVLGEVKKEYCDLLRRADAIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYEFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
>Mature_516_residues
TKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGPESVTEANSPRAPQYVFDSGVP
VFGVCYGMQTMAEQLGGRVATSDEREFGYAQVKISGESALFKDLDLTQDVWMSHGDKVVEIPADFVKIGETDTCPYAAMA
NEEKKYYGVQFHPEVTHTKNGLQMLENFVLGVCGCERLWTSESIIEDAVARIKEQVGNDEVILGLSGGVDSSVVAMLVHR
AIGSKLTCVFVDNGLLRLNEGEQVMEMFGDKFGLNIIKVDAEERFLKALEGIDEPEAKRKTIGRVFVEVFDEESKKLSNA
KWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMKMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLG
VRVLGEVKKEYCDLLRRADAIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLP
YEFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=554, Percent_Identity=35.5595667870036, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=80.7619047619048, Blast_Score=889, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992717, Length=569, Percent_Identity=35.1493848857645, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71992710, Length=569, Percent_Identity=35.1493848857645, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI133901714, Length=569, Percent_Identity=35.1493848857645, Blast_Score=301, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6323873, Length=515, Percent_Identity=49.3203883495146, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322638, Length=229, Percent_Identity=25.764192139738, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281365319, Length=555, Percent_Identity=37.4774774774775, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 57765; Mature: 57633

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCCCCEEEECCCC
ESVTEANSPRAPQYVFDSGVPVFGVCYGMQTMAEQLGGRVATSDEREFGYAQVKISGESA
CHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCCH
LFKDLDLTQDVWMSHGDKVVEIPADFVKIGETDTCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHCCCCHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGLQMLENFVLGVCGCERLWTSESIIEDAVARIKEQVGNDEVILGLSGGVDSSVVAMLVH
HHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGSKLTCVFVDNGLLRLNEGEQVMEMFGDKFGLNIIKVDAEERFLKALEGIDEPEAKR
HHHCCCEEEEEECCCEEEECCCHHHHHHCCHHHCCEEEEECHHHHHHHHHCCCCCCHHHH
KTIGRVFVEVFDEESKKLSNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
KMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYEFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC
>Mature Secondary Structure 
TKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCCCCEEEECCCC
ESVTEANSPRAPQYVFDSGVPVFGVCYGMQTMAEQLGGRVATSDEREFGYAQVKISGESA
CHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCCH
LFKDLDLTQDVWMSHGDKVVEIPADFVKIGETDTCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHCCCCHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGLQMLENFVLGVCGCERLWTSESIIEDAVARIKEQVGNDEVILGLSGGVDSSVVAMLVH
HHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGSKLTCVFVDNGLLRLNEGEQVMEMFGDKFGLNIIKVDAEERFLKALEGIDEPEAKR
HHHCCCEEEEEECCCEEEECCCHHHHHHCCHHHCCEEEEECHHHHHHHHHCCCCCCHHHH
KTIGRVFVEVFDEESKKLSNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
KMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYEFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA