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The map label for this gene is engA

Identifier: 147675115

GI number: 147675115

Start: 306242

End: 307726

Strand: Direct

Name: engA

Synonym: VC0395_A0292

Alternate gene names: 147675115

Gene position: 306242-307726 (Clockwise)

Preceding gene: 147675122

Following gene: 147675742

Centisome position: 10.13

GC content: 49.23

Gene sequence:

>1485_bases
ATGGTACCTGTTGTTGCTCTTGTTGGGCGTCCAAACGTCGGTAAATCGACGCTGTTTAATCGACTCACTCGCACACGGGA
CGCATTAGTCGCGGATTTTCCCGGCTTAACGCGTGATCGCAAATACGGTCAAGCTAAGTTGGGTGAGCACGAATTTATCG
TGATCGATACCGGCGGTATCGATGGCTCTGAAGAAGGCGTTGAGACCAAAATGGCACAGCAATCGCTCGCTGCGATTGAC
GAAGCTGATGTGGTGCTGTTTATGGTGGATGGCCGCGCGGGTCTGACGGTTGCAGATGAAGCGATTGCTCAGCATCTGCG
CCGTATTGAAAAGCCAGCAATTTTGGTTGTGAACAAAGTGGATGGTATCGATGCGGATGCCGCTTCGGCGGAGTTCTGGC
AACTCGGTATGGATCAGATGTACCAGATCGCCGCCGCGCATGGCCGTGGTGTGGGCGCATTGATTGACCGTGTGCTGAAT
CCGTTTGCTGAGCAAATGGAATCTGAGCAAGCTCAACTTGAAGATCTGACCAACGAAGAAGATCCAGAAGAAGAGCAGTT
AGAGTACAGCGAAGAAGAAGCGGAAGCCGAATACAAGCGCCTGCAAGACTTGCCGATTAAACTCGCGATCATCGGTCGTC
CAAACGTGGGTAAATCAACACTGACTAACCGCATTCTGGGTGAAGAGCGAGTCGTGGTTTACGATATGCCGGGAACCACG
CGTGACTCTATCTACATCCCAATGAAACGTGATGAGCGTGAATATGTGTTGATTGATACTGCGGGCGTGCGTCGCCGTAA
GCGTATCAATGAAACCGTTGAGAAGTTCTCGGTAGTCAAAACCTTACAAGCGATTGAAGATGCTAACGTGGTATTGTTGG
TGGTCGACGCGCGTGAGAACATCTCAGATCAAGATCTTAGCCTACTTGGTTTTGCCCTAAATTCTGGCCGCTCCATTGTA
ATTGCGGTGAATAAGTGGGATGGTCTGAGTTTTGACGTCAAAGAACACGTGAAGAAAGAGCTAGACCGTCGACTGGGTTT
CGTTGATTTTGCACGCATCCACTTTATTTCTGCACTGCATGGCACAGGTGTCGGTCACTTGTTTGAATCGGTGCAAGAAG
CGTATCGCTCTGCAACCACGCGTGTGGGAACGTCTGTACTGACTCGTATTATGAAAATGGCCACCGATGATCACCAGCCG
CCTATGGTGCGTGGGCGTCGTGTGAAACTGAAATATGCACACGCGGGTGGTTACAACCCACCGATCATCGTTATCCACGG
TAACCAAGTCAACGAGCTGCCAGATTCATACAAACGTTATTTGATGAACTATTACCGTAAATCGCTAGAAATCATGGGTA
CACCAATTCGTATCCAGTTCCAAAATAGCGAAAACCCATTTGAAGGTAAGACGAATAAAATGACCCTCTCACAAGAGCGA
CAACGTAAGCGATTGATGAGCATGGTGAAAAACCGCAGAAAATAA

Upstream 100 bases:

>100_bases
ATCATAATGATGATAATGAAAGTGGTTATAGGTAAAAATTTAGCGCTTGTGACAGAGTGTTTACCTATAACTACATAAAT
GAAAGTTTGTAGAGGTTGTT

Downstream 100 bases:

>100_bases
TTTATAAAGCCCAAGTTTACTTGGGCTTTTTTGTCTCTGAAGGAGAGAGATGCATGACCTATTGCGCAACAGAAGTCACC
TTTCCACAAGGAATTACATC

Product: GTP-binding protein EngA

Products: NA

Alternate protein names: GTP-binding protein EngA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGQAKLGEHEFIVIDTGGIDGSEEGVETKMAQQSLAAID
EADVVLFMVDGRAGLTVADEAIAQHLRRIEKPAILVVNKVDGIDADAASAEFWQLGMDQMYQIAAAHGRGVGALIDRVLN
PFAEQMESEQAQLEDLTNEEDPEEEQLEYSEEEAEAEYKRLQDLPIKLAIIGRPNVGKSTLTNRILGEERVVVYDMPGTT
RDSIYIPMKRDEREYVLIDTAGVRRRKRINETVEKFSVVKTLQAIEDANVVLLVVDARENISDQDLSLLGFALNSGRSIV
IAVNKWDGLSFDVKEHVKKELDRRLGFVDFARIHFISALHGTGVGHLFESVQEAYRSATTRVGTSVLTRIMKMATDDHQP
PMVRGRRVKLKYAHAGGYNPPIIVIHGNQVNELPDSYKRYLMNYYRKSLEIMGTPIRIQFQNSENPFEGKTNKMTLSQER
QRKRLMSMVKNRRK

Sequences:

>Translated_494_residues
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGQAKLGEHEFIVIDTGGIDGSEEGVETKMAQQSLAAID
EADVVLFMVDGRAGLTVADEAIAQHLRRIEKPAILVVNKVDGIDADAASAEFWQLGMDQMYQIAAAHGRGVGALIDRVLN
PFAEQMESEQAQLEDLTNEEDPEEEQLEYSEEEAEAEYKRLQDLPIKLAIIGRPNVGKSTLTNRILGEERVVVYDMPGTT
RDSIYIPMKRDEREYVLIDTAGVRRRKRINETVEKFSVVKTLQAIEDANVVLLVVDARENISDQDLSLLGFALNSGRSIV
IAVNKWDGLSFDVKEHVKKELDRRLGFVDFARIHFISALHGTGVGHLFESVQEAYRSATTRVGTSVLTRIMKMATDDHQP
PMVRGRRVKLKYAHAGGYNPPIIVIHGNQVNELPDSYKRYLMNYYRKSLEIMGTPIRIQFQNSENPFEGKTNKMTLSQER
QRKRLMSMVKNRRK
>Mature_494_residues
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGQAKLGEHEFIVIDTGGIDGSEEGVETKMAQQSLAAID
EADVVLFMVDGRAGLTVADEAIAQHLRRIEKPAILVVNKVDGIDADAASAEFWQLGMDQMYQIAAAHGRGVGALIDRVLN
PFAEQMESEQAQLEDLTNEEDPEEEQLEYSEEEAEAEYKRLQDLPIKLAIIGRPNVGKSTLTNRILGEERVVVYDMPGTT
RDSIYIPMKRDEREYVLIDTAGVRRRKRINETVEKFSVVKTLQAIEDANVVLLVVDARENISDQDLSLLGFALNSGRSIV
IAVNKWDGLSFDVKEHVKKELDRRLGFVDFARIHFISALHGTGVGHLFESVQEAYRSATTRVGTSVLTRIMKMATDDHQP
PMVRGRRVKLKYAHAGGYNPPIIVIHGNQVNELPDSYKRYLMNYYRKSLEIMGTPIRIQFQNSENPFEGKTNKMTLSQER
QRKRLMSMVKNRRK

Specific function: GTPase that plays an essential role in the late steps of ribosome biogenesis

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH-like domain

Homologues:

Organism=Escherichia coli, GI87082120, Length=500, Percent_Identity=71.2, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1788919, Length=137, Percent_Identity=34.3065693430657, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI2367268, Length=104, Percent_Identity=39.4230769230769, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323665, Length=144, Percent_Identity=31.9444444444444, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DER_VIBC3 (A5F3E6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3E6
- SMR:   A5F3E6
- STRING:   A5F3E6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0292
- eggNOG:   COG1160
- HOGENOM:   HBG592135
- OMA:   KCEKAFD
- ProtClustDB:   PRK00093
- BioCyc:   VCHO345073:VC0395_A0292-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00195
- InterPro:   IPR003593
- InterPro:   IPR016484
- InterPro:   IPR006073
- InterPro:   IPR015946
- InterPro:   IPR002917
- InterPro:   IPR005225
- Gene3D:   G3DSA:3.30.300.20
- PIRSF:   PIRSF006485
- PRINTS:   PR00326
- SMART:   SM00382
- TIGRFAMs:   TIGR03594
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1

EC number: NA

Molecular weight: Translated: 55639; Mature: 55639

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGQAKLGEHEFIVIDTGGI
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCC
DGSEEGVETKMAQQSLAAIDEADVVLFMVDGRAGLTVADEAIAQHLRRIEKPAILVVNKV
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEEEECC
DGIDADAASAEFWQLGMDQMYQIAAAHGRGVGALIDRVLNPFAEQMESEQAQLEDLTNEE
CCCCCCCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DPEEEQLEYSEEEAEAEYKRLQDLPIKLAIIGRPNVGKSTLTNRILGEERVVVYDMPGTT
CCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCC
RDSIYIPMKRDEREYVLIDTAGVRRRKRINETVEKFSVVKTLQAIEDANVVLLVVDAREN
CCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
ISDQDLSLLGFALNSGRSIVIAVNKWDGLSFDVKEHVKKELDRRLGFVDFARIHFISALH
CCCCCHHHHEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GTGVGHLFESVQEAYRSATTRVGTSVLTRIMKMATDDHQPPMVRGRRVKLKYAHAGGYNP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCC
PIIVIHGNQVNELPDSYKRYLMNYYRKSLEIMGTPIRIQFQNSENPFEGKTNKMTLSQER
CEEEEECCCCHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEHHHHH
QRKRLMSMVKNRRK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGQAKLGEHEFIVIDTGGI
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCC
DGSEEGVETKMAQQSLAAIDEADVVLFMVDGRAGLTVADEAIAQHLRRIEKPAILVVNKV
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEEEECC
DGIDADAASAEFWQLGMDQMYQIAAAHGRGVGALIDRVLNPFAEQMESEQAQLEDLTNEE
CCCCCCCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DPEEEQLEYSEEEAEAEYKRLQDLPIKLAIIGRPNVGKSTLTNRILGEERVVVYDMPGTT
CCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCC
RDSIYIPMKRDEREYVLIDTAGVRRRKRINETVEKFSVVKTLQAIEDANVVLLVVDAREN
CCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
ISDQDLSLLGFALNSGRSIVIAVNKWDGLSFDVKEHVKKELDRRLGFVDFARIHFISALH
CCCCCHHHHEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GTGVGHLFESVQEAYRSATTRVGTSVLTRIMKMATDDHQPPMVRGRRVKLKYAHAGGYNP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCC
PIIVIHGNQVNELPDSYKRYLMNYYRKSLEIMGTPIRIQFQNSENPFEGKTNKMTLSQER
CEEEEECCCCHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEHHHHH
QRKRLMSMVKNRRK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA