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The map label for this gene is nfo

Identifier: 147674847

GI number: 147674847

Start: 2069166

End: 2070026

Strand: Reverse

Name: nfo

Synonym: VC0395_A1939

Alternate gene names: 147674847

Gene position: 2070026-2069166 (Counterclockwise)

Preceding gene: 147673667

Following gene: 147675455

Centisome position: 68.45

GC content: 47.97

Gene sequence:

>861_bases
ATGTATGAGAAGTTAATCGGGGCACATGTATCGGCCTCTGGTGGTGTCGAACTCGCGCCAGTTCGAGCTCATGAAATCGG
AGCGAATGCTTTTGCTCTCTTCACCAAAAACCAGCGCCAATGGGCAGCTAAGCCACTGGAAGCGAGCAGCATTCGTGCTT
TCAAAGCCAATTGTAAAAAATGGGGATTTGGCAGTGAGGCGATTTTGCCGCACGACTCTTATCTGATTAATTTAGGGGCT
CCAGAACCCGAGAAGCTCGACAAATCACGAGCCGCTTTTGTCGATGAAATGCTGCGTTGTGATCAGTTAGGGTTAACTTT
GCTTAACTTTCATCCCGGTAGTCATTTACAGCAGGTCTCGGAAGAGGCGTGTTTAGCGACAATTGCTGAGTCAATTAATC
TTGCGCATCGTCAAGTACCGAATGTGATTGCGGTGATTGAAAATACCGCAGGGCAGGGGAGTAACTTAGGTTGGCGTTTT
GAGCATCTGGCGGCAATCATTGACCAAGTTGAGGATAAAGAGCGGGTTGGCGTATGCCTAGATACTTGCCACACCTTTGC
CGCAGGGTATGATTTGCGTACGAAAGCCGCGTGTGATGAGACATTCGCTGAGTTTGAGCGAGTGGTCGGTATGCACTATT
TGCGCGCAATGCACATCAATGATTCAAAAGGTAAGCTGGCTAGCCGCGTTGACCGCCATCATTCCTTAGGTATGGGAGAG
ATTGGCTGGGAGTGTTTTGAATATATCGCGCAAGATGCTCGATTTAACGGTATCCCTCTGATTTTAGAAACGATTGATCC
TGACATTTGGGCGACTGAGATCGCAACGCTACGCAAATTTTCGACGCAAAAAGAGAATTAA

Upstream 100 bases:

>100_bases
CAAAATTGTAATCCAACTTGACCCGTGCCAGAGAAAATCAGAAAGTTAGCGCTGTGAACTGAAAGTCCGAAGTCATTTAG
CAACAATAAGAAGTCGTAAT

Downstream 100 bases:

>100_bases
TCGGCAAACTTCACAGATTTGGCATCTTTCTTTCATCATGCTCAGGGTCAGCGTTGTAAGAAGGATGCCGCTATGAACTC
ATTAACTCAAACTGCTTCAC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MYEKLIGAHVSASGGVELAPVRAHEIGANAFALFTKNQRQWAAKPLEASSIRAFKANCKKWGFGSEAILPHDSYLINLGA
PEPEKLDKSRAAFVDEMLRCDQLGLTLLNFHPGSHLQQVSEEACLATIAESINLAHRQVPNVIAVIENTAGQGSNLGWRF
EHLAAIIDQVEDKERVGVCLDTCHTFAAGYDLRTKAACDETFAEFERVVGMHYLRAMHINDSKGKLASRVDRHHSLGMGE
IGWECFEYIAQDARFNGIPLILETIDPDIWATEIATLRKFSTQKEN

Sequences:

>Translated_286_residues
MYEKLIGAHVSASGGVELAPVRAHEIGANAFALFTKNQRQWAAKPLEASSIRAFKANCKKWGFGSEAILPHDSYLINLGA
PEPEKLDKSRAAFVDEMLRCDQLGLTLLNFHPGSHLQQVSEEACLATIAESINLAHRQVPNVIAVIENTAGQGSNLGWRF
EHLAAIIDQVEDKERVGVCLDTCHTFAAGYDLRTKAACDETFAEFERVVGMHYLRAMHINDSKGKLASRVDRHHSLGMGE
IGWECFEYIAQDARFNGIPLILETIDPDIWATEIATLRKFSTQKEN
>Mature_286_residues
MYEKLIGAHVSASGGVELAPVRAHEIGANAFALFTKNQRQWAAKPLEASSIRAFKANCKKWGFGSEAILPHDSYLINLGA
PEPEKLDKSRAAFVDEMLRCDQLGLTLLNFHPGSHLQQVSEEACLATIAESINLAHRQVPNVIAVIENTAGQGSNLGWRF
EHLAAIIDQVEDKERVGVCLDTCHTFAAGYDLRTKAACDETFAEFERVVGMHYLRAMHINDSKGKLASRVDRHHSLGMGE
IGWECFEYIAQDARFNGIPLILETIDPDIWATEIATLRKFSTQKEN

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=281, Percent_Identity=68.6832740213523, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=48.2758620689655, Blast_Score=273, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6322735, Length=260, Percent_Identity=41.1538461538462, Blast_Score=219, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_VIBC3 (A5F5R6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F5R6
- SMR:   A5F5R6
- STRING:   A5F5R6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1939
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   VCHO345073:VC0395_A1939-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31702; Mature: 31702

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEKLIGAHVSASGGVELAPVRAHEIGANAFALFTKNQRQWAAKPLEASSIRAFKANCKK
CCCHHHCCCCCCCCCEEEEEEHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCHH
WGFGSEAILPHDSYLINLGAPEPEKLDKSRAAFVDEMLRCDQLGLTLLNFHPGSHLQQVS
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHH
EEACLATIAESINLAHRQVPNVIAVIENTAGQGSNLGWRFEHLAAIIDQVEDKERVGVCL
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
DTCHTFAAGYDLRTKAACDETFAEFERVVGMHYLRAMHINDSKGKLASRVDRHHSLGMGE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCH
IGWECFEYIAQDARFNGIPLILETIDPDIWATEIATLRKFSTQKEN
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYEKLIGAHVSASGGVELAPVRAHEIGANAFALFTKNQRQWAAKPLEASSIRAFKANCKK
CCCHHHCCCCCCCCCEEEEEEHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCHH
WGFGSEAILPHDSYLINLGAPEPEKLDKSRAAFVDEMLRCDQLGLTLLNFHPGSHLQQVS
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHH
EEACLATIAESINLAHRQVPNVIAVIENTAGQGSNLGWRFEHLAAIIDQVEDKERVGVCL
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
DTCHTFAAGYDLRTKAACDETFAEFERVVGMHYLRAMHINDSKGKLASRVDRHHSLGMGE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCH
IGWECFEYIAQDARFNGIPLILETIDPDIWATEIATLRKFSTQKEN
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA