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The map label for this gene is pnp

Identifier: 147674727

GI number: 147674727

Start: 181032

End: 183170

Strand: Direct

Name: pnp

Synonym: VC0395_A0178

Alternate gene names: 147674727

Gene position: 181032-183170 (Clockwise)

Preceding gene: 147675292

Following gene: 147674411

Centisome position: 5.99

GC content: 49.56

Gene sequence:

>2139_bases
ATGTTCGAAAAACCAGTAGTAAAAACGTTTCAGTACGGTAACCACACCGTTACTCTCGAAACGGGTGTAATGGCTCGCCA
AGCAACGGCAGCAGTAATGGCTACCATGGATGACACTGCGGTGTTTGTCTCTGTGGTCGGTAAGAAAGAAGCGGTAGTGG
GTCAAGATTTCTTCCCTCTGACTGTAAACTACCAAGAGCGTACTTACGCGGCAGGTAAAATCCCTGGCGGTTTCTTTAAG
CGCGAAGGCCGTCCTTCTGAAGGCGAAACGCTGATTGCTCGTCTGATTGACCGTCCAATTCGTCCGCTGTTCCCTGATGG
TTTCACTAACGAAGTGCAAGTGATCGCGACTGTGGTTTCAGTAAACCCAGACGTGCAACCTGACATCATCTCGATGATCG
GTACTTCTGCAGCACTGGCGATTTCTGGACTCCCATTCAATGGTCCAATCGGTGCGGCTCGCGTAGGTCACATTGATGGT
CAATTGGTTCTGAACCCAAGTGAAAAAGAGCTGAAACAGTCTCGTTTGGATCTGGTGGTTGCGGGTACTGACAACGCAGT
ACTGATGGTGGAATCAGAAGCACAAATTCTGACTGAAGAAGAGATGCTGGCTGCCGTTGTGTTTGGTCATGATCAACAAC
AAGCCGTGATCAAAGCAATCAATGAATTCGCAGCGGAAGTGGCAACACCAGCATGGGAATGGGTTGCACCAGCCGAGAAC
ACTGAGCTGAAAGCGAAAGTGGCTGCTCTGGCAGAAACTCGTTTGGTGGAAGCGTACCAAATCACTGAGAAAATGGCGCG
TTATGATCGTATCCATGCAATCAGTTCAGAAGTGACGGCGGCTCTGCTGGCTGAAAACGAAGCTTTAGACACCAAAGAAA
TTCACACCATTTTCCACGATCTGGAAAAAACCGTTGTTCGTCGCAGCATCATCGCGGGTAACCCACGTATCGATGGCCGT
GAAAAAGACATGGTTCGTGCTCTGGACGTTCGTACTGGCGTTCTACCACGTACTCACGGCTCTGCTCTGTTCACACGTGG
TGAAACTCAGGCTCTGGTTACAGCAACTCTAGGCACTCAACGTGATGCGCAAATCATCGATGAGCTGACTGGCGAGAAGA
AAGATCACTTCCTACTGCACTACAACTTCCCTCCATACTGTGTGGGTGAAACGGGTTTTGTGGGTTCTCCTAAGCGTCGT
GAAATCGGTCACGGCCGTTTGGCGAAGCGTGGTATTGCTGCGGTTATGCCATCACCAGAAGAGTTCCCGTACACAGTTCG
TGTCGTTTCTGAAATCACGGAATCTAACGGTTCATCTTCAATGGCTTCTGTGTGTGGTTCATCGCTTGCTCTGATGGATG
CTGGTGTTCCAATCAAGGCTTCTGTTGCGGGTATCGCAATGGGTCTGGTGAAAGAAGAAAATGATTTTGTGGTTCTGTCT
GACATTCTGGGTGATGAAGATCACCTAGGCGACATGGACTTTAAAGTCGCAGGTACCGCGACAGGTGTTACTGCACTGCA
GATGGACATCAAGATCGAAGGCATCACCAAAGAGATCATGCAAATCGCTCTGAACCAAGCGAAAGGTGCTCGTCTACACA
TTCTGTCTGTGATGGATCAAGCGATCTCTGCAGCGCGTAGCGATATCTCTGAATTTGCGCCACGTATCCACACGATGAAG
ATCAGCGTTGAGAAGATCAAAGACGTGATCGGTAAAGGTGGTGCGGTAATTCGTCAGTTGACGGAAGAAACGGGTACCAC
CATCGAGATCGAAGATGACGGCACGATCAAGATTGCTGCGACTGACGGCGATCAAGCGAAAGAAGCGATCCGCCGTATTC
AAGAGATCACCGCCGAAGTTGAAGTGGGCGTGATCTACACTGGTAAAGTAGCTCGTTTGGCAGACTTTGGTGCGTTTGTA
ACGATTCTTCCTGGTAAAGATGGTCTAGTACACATCTCTCAAATCGCTGACAAGCGCGTAGAGAAAGTGTCTGACTACCT
GACTGAAGGCCAAGAAGTTCAAGTTAAAGTGCTGGAAATCGACCGCCAAGGCCGTGTACGTCTAAGCATGAAAGAAGCGG
TTGAAACTTCTGATGCAGCAGCAGAAGTGGCACCACAAGCGGCACCACAAGCGGAATAA

Upstream 100 bases:

>100_bases
GTACTCCCAAGTTGTCAGTCTATTTTTACTAGTCGCGATTAGTAGTGCTCTGAGTATTAATGATTTGATTTCAGGCTTGT
CCTGAGAATAAGGAATACTC

Downstream 100 bases:

>100_bases
TTTTCCGTTGCTAGAGCAAAAACAACACGGCTTTTACTTTTCATAATAAAAGCGTGCTATAAAGGGAGCGTTATTCGCTC
CCTTTTTTATGCGAAATCCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVSVNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDG
QLVLNPSEKELKQSRLDLVVAGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHAISSEVTAALLAENEALDTKEIHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGRLAKRGIAAVMPSPEEFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQAISAARSDISEFAPRIHTMK
ISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAATDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAEVAPQAAPQAE

Sequences:

>Translated_712_residues
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVSVNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDG
QLVLNPSEKELKQSRLDLVVAGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHAISSEVTAALLAENEALDTKEIHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGRLAKRGIAAVMPSPEEFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQAISAARSDISEFAPRIHTMK
ISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAATDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAEVAPQAAPQAE
>Mature_712_residues
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVSVNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDG
QLVLNPSEKELKQSRLDLVVAGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHAISSEVTAALLAENEALDTKEIHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGRLAKRGIAAVMPSPEEFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQAISAARSDISEFAPRIHTMK
ISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAATDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAEVAPQAAPQAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=706, Percent_Identity=37.8186968838527, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=76.9452449567723, Blast_Score=1107, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=666, Percent_Identity=32.8828828828829, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17535281, Length=80, Percent_Identity=46.25, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281362905, Length=714, Percent_Identity=38.375350140056, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=714, Percent_Identity=38.375350140056, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=714, Percent_Identity=38.375350140056, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=38.5321100917431, Blast_Score=429, Evalue=1e-120,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_VIBC3 (A5F913)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F913
- STRING:   A5F913
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0178
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   VCHO345073:VC0395_A0178-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76841; Mature: 76841

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPL
CCCCCCHHHEECCCEEEEEECCCHHHHHHHHHEEECCCCEEEEEECCCCCEEECCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
VNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDGQLVLNPSEKELKQSRLDLVV
CCCCCCHHHHHHHCCCHHEEEECCCCCCCCCCCEEECCCCEEEECCCHHHHHHCCCCEEE
AGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
ECCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
TELKAKVAALAETRLVEAYQITEKMARYDRIHAISSEVTAALLAENEALDTKEIHTIFHD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEECCCC
RDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGIAAVMPSPE
CHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCC
EFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
CCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHEECCCCEEEEH
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQ
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCEEHHHHHHHH
AISAARSDISEFAPRIHTMKISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAA
HHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFVTILPGKDGLVHISQIADKRV
CCCHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHCCCCEEEEECCCCCCEEHHHHHHHHH
EKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAEVAPQAAPQAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPL
CCCCCCHHHEECCCEEEEEECCCHHHHHHHHHEEECCCCEEEEEECCCCCEEECCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
VNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDGQLVLNPSEKELKQSRLDLVV
CCCCCCHHHHHHHCCCHHEEEECCCCCCCCCCCEEECCCCEEEECCCHHHHHHCCCCEEE
AGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
ECCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
TELKAKVAALAETRLVEAYQITEKMARYDRIHAISSEVTAALLAENEALDTKEIHTIFHD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEECCCC
RDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGIAAVMPSPE
CHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCC
EFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
CCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHEECCCCEEEEH
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQ
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCEEHHHHHHHH
AISAARSDISEFAPRIHTMKISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAA
HHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFVTILPGKDGLVHISQIADKRV
CCCHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHCCCCEEEEECCCCCCEEHHHHHHHHH
EKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAEVAPQAAPQAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA