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The map label for this gene is recJ [H]

Identifier: 147674577

GI number: 147674577

Start: 2144046

End: 2145800

Strand: Reverse

Name: recJ [H]

Synonym: VC0395_A1993

Alternate gene names: 147674577

Gene position: 2145800-2144046 (Counterclockwise)

Preceding gene: 147674865

Following gene: 147673255

Centisome position: 70.96

GC content: 51.45

Gene sequence:

>1755_bases
TTGGCCTGTCACAGCATGATAGAAATCCAGCGACGCCCCGAACCTGATCTCTCACTTCTCCCCGACCACATCGACCCACT
GTTACGTCGGCTTTACCTGAGCCGTGGTGTAAGCAACGTGGCACAACTTGAAAAAGCCGCCAAAGCGCTGCACTCATTCA
AGCAGTTACATGGTATTGATCGTGCGGTTGAGTTGCTGTTCGCAGCGATTGCTGAGCAAAAACGCATCATAGTCGTCGGT
GATTTTGATGCCGATGGCGCAACCAGTTCGGCACTTTCCGTGCTCGCGCTGCGTATGCTCGGTAGTCGTAATGTCGATTA
CTTAGTGCCAAACCGTTTTGAAGATGGTTATGGTCTGAGCCCTGAAGTGGTTGATCAAGCGATTGAGCGTGGCGCAGAGA
TGATCATGACCGTGGATAACGGCGTTTCATCACTGGAAGGGGTGCGTTACGCCAAACAGAAAGGGCTGCAAGTGGTGGTC
ACCGATCATCACTTACCGGGACAAGAACTCCCTGAAGTCGATGCCATGGTAAACCCGAACTTGCAGCAGTGTGCTTTTCC
TTCTAAGGCGCTGGCTGGGGTGGGGGTTGCTTTCTATTTGATGATGGCACTTTGTGTGCACATGCGTCAGCAAGGTTGGT
TTGCCGCGCAAGGCATGGTTGAACCGAAATTGATGGAACTGATCGATTTAGTGGCACTCGGTACGGTCGCCGATGTGGTG
CCACTGGATGACAACAATCGTATTTTGGTGCACCAAGGTTTGCAGCGTATTCGTGCGGGTTTAGCGCGCCCCGGTATTCA
AGCTCTGATTGAAGTCGCCAAACGGGATGCGCGCCGTTTGGTCGCTGCTGATTTTGGCTTTGCGCTTGGACCACGGATTA
ACGCCGCAGGGCGTTTGGATGATATGTCGTTCGGCGTTGAACTTTTGCTGTGTAACAACATTCACGCTGCGCGGCGTATG
GCGAGTGAGCTGGACGGCCTCAATCAAACCCGAAAAGAGATTGAAGAGGGGATGAAGCAAGAGGCGGTCGCGTTTTGTGA
GCGTTTGCAACTGAGCGATGATCGCGAGCTGCCTTACGGTTTAGTGCTGTTTCAGCGCGATTGGCACCAAGGGGTGATCG
GCATTCTTGCTTCGCGCATCAAAGAGAAATACCACCGACCCGTGATTGCGTTTGCTGAAGGTGGTGATGGCAAGCTAAAA
GGTTCATGTCGCTCGGTTCCCGGTCTGCATATGCGTGATGCACTAGATAAAATTGATACGCAAAACCCGGGCATCATACT
CAAATTTGGTGGTCACGCGATGGCGGCTGGATTAACCATTGAAGAGCAAAATTTTGAACATTTTTGCCGATTGTTTGACC
AGCTAGTGCGTGAAGAGCTGGATGAAGCAGCACTCCAAGGCATCATCCTCAGCGATGGCGAACTGAAACCGGAAGAGTTT
TCGCTGCATACCGCCGAATTACTGCGTGCCGGTGGCCCGTGGGGGCAAGCGTTTCCTGAACCCCTATTTGATGGTGAATT
TAAAGTGCTGCACCAAAAACTGGTTGGCGAAAAACACCTCAAACTGATGCTTGAGCCGCTGTATAAAGGCTTTCCAACCA
ATGTGATGATCGACGGGATTGCGTTTAATGTCGACCTACGCCGCTGGCCGGATGTCTCAGTCAAAACGGTACGTTTGGCG
TACAAACTCGACATCAACGAGTTTCGCGGTAATCAATCATTGCAACTCATGATTGATTATCTGGAAGCGAAATGA

Upstream 100 bases:

>100_bases
GTTGGCGGCTATTTGCCTGCGCCGCAATTGCTGCAACGCCTGCAAGCCACACAATAGTTTTTCATTTTCGACTAATTAAG
GAAACAGGCCAGAGGCAACT

Downstream 100 bases:

>100_bases
TTGAAAACAACGCCCTCTCTCATAAAAAGAGGGCGTTTTCGTTATTAAGGATTAAGTGCTTCCAACAAAATCAGTTCACG
TTTTAGCGTGGTTTTTTCAT

Product: ssDNA exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MACHSMIEIQRRPEPDLSLLPDHIDPLLRRLYLSRGVSNVAQLEKAAKALHSFKQLHGIDRAVELLFAAIAEQKRIIVVG
DFDADGATSSALSVLALRMLGSRNVDYLVPNRFEDGYGLSPEVVDQAIERGAEMIMTVDNGVSSLEGVRYAKQKGLQVVV
TDHHLPGQELPEVDAMVNPNLQQCAFPSKALAGVGVAFYLMMALCVHMRQQGWFAAQGMVEPKLMELIDLVALGTVADVV
PLDDNNRILVHQGLQRIRAGLARPGIQALIEVAKRDARRLVAADFGFALGPRINAAGRLDDMSFGVELLLCNNIHAARRM
ASELDGLNQTRKEIEEGMKQEAVAFCERLQLSDDRELPYGLVLFQRDWHQGVIGILASRIKEKYHRPVIAFAEGGDGKLK
GSCRSVPGLHMRDALDKIDTQNPGIILKFGGHAMAAGLTIEEQNFEHFCRLFDQLVREELDEAALQGIILSDGELKPEEF
SLHTAELLRAGGPWGQAFPEPLFDGEFKVLHQKLVGEKHLKLMLEPLYKGFPTNVMIDGIAFNVDLRRWPDVSVKTVRLA
YKLDINEFRGNQSLQLMIDYLEAK

Sequences:

>Translated_584_residues
MACHSMIEIQRRPEPDLSLLPDHIDPLLRRLYLSRGVSNVAQLEKAAKALHSFKQLHGIDRAVELLFAAIAEQKRIIVVG
DFDADGATSSALSVLALRMLGSRNVDYLVPNRFEDGYGLSPEVVDQAIERGAEMIMTVDNGVSSLEGVRYAKQKGLQVVV
TDHHLPGQELPEVDAMVNPNLQQCAFPSKALAGVGVAFYLMMALCVHMRQQGWFAAQGMVEPKLMELIDLVALGTVADVV
PLDDNNRILVHQGLQRIRAGLARPGIQALIEVAKRDARRLVAADFGFALGPRINAAGRLDDMSFGVELLLCNNIHAARRM
ASELDGLNQTRKEIEEGMKQEAVAFCERLQLSDDRELPYGLVLFQRDWHQGVIGILASRIKEKYHRPVIAFAEGGDGKLK
GSCRSVPGLHMRDALDKIDTQNPGIILKFGGHAMAAGLTIEEQNFEHFCRLFDQLVREELDEAALQGIILSDGELKPEEF
SLHTAELLRAGGPWGQAFPEPLFDGEFKVLHQKLVGEKHLKLMLEPLYKGFPTNVMIDGIAFNVDLRRWPDVSVKTVRLA
YKLDINEFRGNQSLQLMIDYLEAK
>Mature_583_residues
ACHSMIEIQRRPEPDLSLLPDHIDPLLRRLYLSRGVSNVAQLEKAAKALHSFKQLHGIDRAVELLFAAIAEQKRIIVVGD
FDADGATSSALSVLALRMLGSRNVDYLVPNRFEDGYGLSPEVVDQAIERGAEMIMTVDNGVSSLEGVRYAKQKGLQVVVT
DHHLPGQELPEVDAMVNPNLQQCAFPSKALAGVGVAFYLMMALCVHMRQQGWFAAQGMVEPKLMELIDLVALGTVADVVP
LDDNNRILVHQGLQRIRAGLARPGIQALIEVAKRDARRLVAADFGFALGPRINAAGRLDDMSFGVELLLCNNIHAARRMA
SELDGLNQTRKEIEEGMKQEAVAFCERLQLSDDRELPYGLVLFQRDWHQGVIGILASRIKEKYHRPVIAFAEGGDGKLKG
SCRSVPGLHMRDALDKIDTQNPGIILKFGGHAMAAGLTIEEQNFEHFCRLFDQLVREELDEAALQGIILSDGELKPEEFS
LHTAELLRAGGPWGQAFPEPLFDGEFKVLHQKLVGEKHLKLMLEPLYKGFPTNVMIDGIAFNVDLRRWPDVSVKTVRLAY
KLDINEFRGNQSLQLMIDYLEAK

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=575, Percent_Identity=63.304347826087, Blast_Score=745, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 64866; Mature: 64735

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACHSMIEIQRRPEPDLSLLPDHIDPLLRRLYLSRGVSNVAQLEKAAKALHSFKQLHGID
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RAVELLFAAIAEQKRIIVVGDFDADGATSSALSVLALRMLGSRNVDYLVPNRFEDGYGLS
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCC
PEVVDQAIERGAEMIMTVDNGVSSLEGVRYAKQKGLQVVVTDHHLPGQELPEVDAMVNPN
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHCCCC
LQQCAFPSKALAGVGVAFYLMMALCVHMRQQGWFAAQGMVEPKLMELIDLVALGTVADVV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHEE
PLDDNNRILVHQGLQRIRAGLARPGIQALIEVAKRDARRLVAADFGFALGPRINAAGRLD
ECCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DMSFGVELLLCNNIHAARRMASELDGLNQTRKEIEEGMKQEAVAFCERLQLSDDRELPYG
CHHCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LVLFQRDWHQGVIGILASRIKEKYHRPVIAFAEGGDGKLKGSCRSVPGLHMRDALDKIDT
EEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
QNPGIILKFGGHAMAAGLTIEEQNFEHFCRLFDQLVREELDEAALQGIILSDGELKPEEF
CCCCEEEEECCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHH
SLHTAELLRAGGPWGQAFPEPLFDGEFKVLHQKLVGEKHLKLMLEPLYKGFPTNVMIDGI
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEE
AFNVDLRRWPDVSVKTVRLAYKLDINEFRGNQSLQLMIDYLEAK
EEEEECCCCCCCCEEEEEEEEEEEHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ACHSMIEIQRRPEPDLSLLPDHIDPLLRRLYLSRGVSNVAQLEKAAKALHSFKQLHGID
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RAVELLFAAIAEQKRIIVVGDFDADGATSSALSVLALRMLGSRNVDYLVPNRFEDGYGLS
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCC
PEVVDQAIERGAEMIMTVDNGVSSLEGVRYAKQKGLQVVVTDHHLPGQELPEVDAMVNPN
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHCCCC
LQQCAFPSKALAGVGVAFYLMMALCVHMRQQGWFAAQGMVEPKLMELIDLVALGTVADVV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHEE
PLDDNNRILVHQGLQRIRAGLARPGIQALIEVAKRDARRLVAADFGFALGPRINAAGRLD
ECCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DMSFGVELLLCNNIHAARRMASELDGLNQTRKEIEEGMKQEAVAFCERLQLSDDRELPYG
CHHCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LVLFQRDWHQGVIGILASRIKEKYHRPVIAFAEGGDGKLKGSCRSVPGLHMRDALDKIDT
EEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
QNPGIILKFGGHAMAAGLTIEEQNFEHFCRLFDQLVREELDEAALQGIILSDGELKPEEF
CCCCEEEEECCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHH
SLHTAELLRAGGPWGQAFPEPLFDGEFKVLHQKLVGEKHLKLMLEPLYKGFPTNVMIDGI
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEE
AFNVDLRRWPDVSVKTVRLAYKLDINEFRGNQSLQLMIDYLEAK
EEEEECCCCCCCCEEEEEEEEEEEHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]