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The map label for this gene is nusA [H]

Identifier: 147674353

GI number: 147674353

Start: 174612

End: 176099

Strand: Direct

Name: nusA [H]

Synonym: VC0395_A0173

Alternate gene names: 147674353

Gene position: 174612-176099 (Clockwise)

Preceding gene: 147673580

Following gene: 147675225

Centisome position: 5.77

GC content: 47.98

Gene sequence:

>1488_bases
ATGAGTAAAGAAATTTTAGCGGTAGTTGAGGCGGTTTCTAACGAGAAAGCGGTACCTCGTGAGCGTATTTTTGAAGCGCT
GGAAACTGCGTTAGCGACTTCAACCAAAAAGAAGTATGAAATCGAGATCGATGTGCGCGTAGCCATCGATCGCAAAACAG
GTGCGTTTGAAACGTTCCGCCGTTGGTTAGTGGTTGAGAATGTTGAGCACCCAACCAAAGAGATCTCATTTGAAGCGGCG
AGCTTTGATGATGACTCGGTTCAACTGGGTGACTATATCGAAGATCAGATCGAATCAGTAACCTTTGACCGTATTACCAC
GCAAACTGCGAAACAAGTGATCGTACAAAAAGTGCGTGAAGCAGAACGTGCACAGATTGTTGAACAATTCATCGATAACG
AAGGTGAATTGGTGACTGGTGTAGTGAAGAAAGTGAACCGTGAAACCGTGATCCTCGATCTGGGTAACAACGCTGAAGCG
GTTATCCTACGTGAAGATCAACTGCCACGCGAAAACTTCCGTCCTGGCGATCGTGTTCGTGGTTTGCTGTACAAAGTGGC
TCCAGAAGCACGTGGTTTCCAACTCTTCATCACTCGTTCTAAGCCAGAAATGTTGGCAGAACTGTTCCGTGTAGAAGTTC
CAGAAATTGGTGAAGAGCTGATTGAGCTGAAAGCGGCTGCGCGTGATCCTGGTTCACGTGCCAAGATCGCGGTAAAAACC
AATGATAAGCGTATCGACCCTGTTGGTGCGTGTGTGGGTATGCGTGGTGCACGTGTACAAGCGGTTTCTGGCGAACTGGG
TGGCGAGCGTATTGATATCGTTTTGTGGGATGATAACCCAGCGCAATTCGTCATTAATGCCATGGCACCAGCCGATGTGG
CATCGATCATTATGGATGAAGATGCACACTCAATGGATATCGCTGTTGAGGCTGATAACCTTGCGCAAGCGATCGGTCGT
AACGGTCAAAACGTACGTTTGGCTTCTCAACTGACCGGTTGGGAACTGAATGTGATGACGGTTGCGGATCTACAGAAGAA
ACACCAAGAAGAGTCAATGGCATCCATCGAGAACTTCATGAAGTATCTCGATATTGAGCAAGATTTTGCTGAGCTGCTGG
TTGAAGAAGGCTTCTCTACGCTAGAAGAGATCGCTTACGTACCAATGAATGAGCTGCTTGATGTTGATGGCATGGATGAA
GATCTGGCAGATGAGCTACGTAGTCGTGCGAAAGAAGCGCTGACGACGATTGCTTTGGCAAAAGAAGAGTCGTTCGAAGG
CCTTGAGCCTGCAGAAGATCTGCTTGCATTAGCAGGACTGGAACGCGACATGGCATTCAAACTGGCAGCGAAAGGTGTTG
CGACACTGGAAGATCTGGCTGACCAAGGTGTGGATGATTTAGAAGGTATTGAAGGTCTGACCGAACAGCGTGCGGGTGAG
CTTATTATGGCGGCTCGTAACATTTGTTGGTTTGGTGAAGACGCATAA

Upstream 100 bases:

>100_bases
CGTAGCCGTGATGGTTGATGGGCAAGAAGAACACTTTGCCTTAAGCAACATTTCAAAAGCTAACCTGATCCCTAAATTTT
AAGTCTTTGAGGCTAGAACA

Downstream 100 bases:

>100_bases
TTTTCAGCAAGGGGAGGAAGCGGCATGACACAAATTACCGTTAAAGCACTGAGTGAAGAAATTGGAACACCAGTAGACCG
CTTATTAGAGCAACTTGCTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSKEILAVVEAVSNEKAVPRERIFEALETALATSTKKKYEIEIDVRVAIDRKTGAFETFRRWLVVENVEHPTKEISFEAA
SFDDDSVQLGDYIEDQIESVTFDRITTQTAKQVIVQKVREAERAQIVEQFIDNEGELVTGVVKKVNRETVILDLGNNAEA
VILREDQLPRENFRPGDRVRGLLYKVAPEARGFQLFITRSKPEMLAELFRVEVPEIGEELIELKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSGELGGERIDIVLWDDNPAQFVINAMAPADVASIIMDEDAHSMDIAVEADNLAQAIGR
NGQNVRLASQLTGWELNVMTVADLQKKHQEESMASIENFMKYLDIEQDFAELLVEEGFSTLEEIAYVPMNELLDVDGMDE
DLADELRSRAKEALTTIALAKEESFEGLEPAEDLLALAGLERDMAFKLAAKGVATLEDLADQGVDDLEGIEGLTEQRAGE
LIMAARNICWFGEDA

Sequences:

>Translated_495_residues
MSKEILAVVEAVSNEKAVPRERIFEALETALATSTKKKYEIEIDVRVAIDRKTGAFETFRRWLVVENVEHPTKEISFEAA
SFDDDSVQLGDYIEDQIESVTFDRITTQTAKQVIVQKVREAERAQIVEQFIDNEGELVTGVVKKVNRETVILDLGNNAEA
VILREDQLPRENFRPGDRVRGLLYKVAPEARGFQLFITRSKPEMLAELFRVEVPEIGEELIELKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSGELGGERIDIVLWDDNPAQFVINAMAPADVASIIMDEDAHSMDIAVEADNLAQAIGR
NGQNVRLASQLTGWELNVMTVADLQKKHQEESMASIENFMKYLDIEQDFAELLVEEGFSTLEEIAYVPMNELLDVDGMDE
DLADELRSRAKEALTTIALAKEESFEGLEPAEDLLALAGLERDMAFKLAAKGVATLEDLADQGVDDLEGIEGLTEQRAGE
LIMAARNICWFGEDA
>Mature_494_residues
SKEILAVVEAVSNEKAVPRERIFEALETALATSTKKKYEIEIDVRVAIDRKTGAFETFRRWLVVENVEHPTKEISFEAAS
FDDDSVQLGDYIEDQIESVTFDRITTQTAKQVIVQKVREAERAQIVEQFIDNEGELVTGVVKKVNRETVILDLGNNAEAV
ILREDQLPRENFRPGDRVRGLLYKVAPEARGFQLFITRSKPEMLAELFRVEVPEIGEELIELKAAARDPGSRAKIAVKTN
DKRIDPVGACVGMRGARVQAVSGELGGERIDIVLWDDNPAQFVINAMAPADVASIIMDEDAHSMDIAVEADNLAQAIGRN
GQNVRLASQLTGWELNVMTVADLQKKHQEESMASIENFMKYLDIEQDFAELLVEEGFSTLEEIAYVPMNELLDVDGMDED
LADELRSRAKEALTTIALAKEESFEGLEPAEDLLALAGLERDMAFKLAAKGVATLEDLADQGVDDLEGIEGLTEQRAGEL
IMAARNICWFGEDA

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=78.989898989899, Blast_Score=803, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54943; Mature: 54812

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEILAVVEAVSNEKAVPRERIFEALETALATSTKKKYEIEIDVRVAIDRKTGAFETFR
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHH
RWLVVENVEHPTKEISFEAASFDDDSVQLGDYIEDQIESVTFDRITTQTAKQVIVQKVRE
HHHHHCCCCCCHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAQIVEQFIDNEGELVTGVVKKVNRETVILDLGNNAEAVILREDQLPRENFRPGDRVR
HHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCHHHH
GLLYKVAPEARGFQLFITRSKPEMLAELFRVEVPEIGEELIELKAAARDPGSRAKIAVKT
HHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSGELGGERIDIVLWDDNPAQFVINAMAPADVASIIMDE
CCCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCHHEEEECCCHHHHHHHHHCC
DAHSMDIAVEADNLAQAIGRNGQNVRLASQLTGWELNVMTVADLQKKHQEESMASIENFM
CCCCEEEEEEHHHHHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KYLDIEQDFAELLVEEGFSTLEEIAYVPMNELLDVDGMDEDLADELRSRAKEALTTIALA
HHHCCHHHHHHHHHHHCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KEESFEGLEPAEDLLALAGLERDMAFKLAAKGVATLEDLADQGVDDLEGIEGLTEQRAGE
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCH
LIMAARNICWFGEDA
HHHHHCCEEECCCCC
>Mature Secondary Structure 
SKEILAVVEAVSNEKAVPRERIFEALETALATSTKKKYEIEIDVRVAIDRKTGAFETFR
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHH
RWLVVENVEHPTKEISFEAASFDDDSVQLGDYIEDQIESVTFDRITTQTAKQVIVQKVRE
HHHHHCCCCCCHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAQIVEQFIDNEGELVTGVVKKVNRETVILDLGNNAEAVILREDQLPRENFRPGDRVR
HHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCHHHH
GLLYKVAPEARGFQLFITRSKPEMLAELFRVEVPEIGEELIELKAAARDPGSRAKIAVKT
HHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSGELGGERIDIVLWDDNPAQFVINAMAPADVASIIMDE
CCCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCHHEEEECCCHHHHHHHHHCC
DAHSMDIAVEADNLAQAIGRNGQNVRLASQLTGWELNVMTVADLQKKHQEESMASIENFM
CCCCEEEEEEHHHHHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KYLDIEQDFAELLVEEGFSTLEEIAYVPMNELLDVDGMDEDLADELRSRAKEALTTIALA
HHHCCHHHHHHHHHHHCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KEESFEGLEPAEDLLALAGLERDMAFKLAAKGVATLEDLADQGVDDLEGIEGLTEQRAGE
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCH
LIMAARNICWFGEDA
HHHHHCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]