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The map label for this gene is rrmJ

Identifier: 147673945

GI number: 147673945

Start: 168161

End: 168790

Strand: Direct

Name: rrmJ

Synonym: VC0395_A0165

Alternate gene names: 147673945

Gene position: 168161-168790 (Clockwise)

Preceding gene: 147675665

Following gene: 147673292

Centisome position: 5.56

GC content: 44.92

Gene sequence:

>630_bases
ATGAGTAAACAGAAACACTCGGCCAGCTCAACTCGCTGGTTAAAAGAGCATTTTGACGACAAATATGTTAACGAAGCAAA
GAAGAAAGGCTATCGTTCACGTGCTATCTTCAAAATAGAAGAGATCCAAAACAAAGATAAACTACTAAAAGCTGGTATGA
CAGTTGTCGATCTTGGCGCTGCACCTGGTGGATGGTCGCAATTTGCGGCCAAAGTAGTTGGCGAAGGTGGGCGTGTTATT
GCATGTGACCTTTTACCTATGGAGTCGATTGCGGGGGTGTCTTTCTTGCAAGGTGATTTCCGTGAGGAAGCGGTGCTCAA
TGCATTGCTCGATCGAATCCAGCCAGATATGGTTGATGTAGTGATGTCTGATATGGCACCTAATATGGCGGGTAACTTGT
CCGTCGATCAGCCTCGCGCCATGTATCTTGTCGAATTGGCACTGGATATGTGTCGACAAGTTCTGGCTCCCAATGGTAGT
TTTGTGGTGAAAGTGTTTCAAGGAGAAGGCTTTGATGATTATGTCAAAGCAGTAAGAGACCTGTTTAAAGTGGTTAAAAT
CCGTAAACCGGACTCCTCTCGATCACGTTCACGAGAAGTTTTTATCGTAGCGACTGGTTACAAAGGTTAA

Upstream 100 bases:

>100_bases
AATATTAGGGTTGAAAACCGTCATTTTAACGCCATCTATGCTAGAAGACTATATTTTCTACCAGACTTTCTTGGATAGTG
GTGACTTAGTTAGGAACAAA

Downstream 100 bases:

>100_bases
CTATTTCACTATTTGGTGCCTAAGTAGACCCTATAGCCGCTAGCTACAAACTGTAGTACCCTACCTTTTAATTACAATTA
GTTATCGCGAGGCTTACACC

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSKQKHSASSTRWLKEHFDDKYVNEAKKKGYRSRAIFKIEEIQNKDKLLKAGMTVVDLGAAPGGWSQFAAKVVGEGGRVI
ACDLLPMESIAGVSFLQGDFREEAVLNALLDRIQPDMVDVVMSDMAPNMAGNLSVDQPRAMYLVELALDMCRQVLAPNGS
FVVKVFQGEGFDDYVKAVRDLFKVVKIRKPDSSRSRSREVFIVATGYKG

Sequences:

>Translated_209_residues
MSKQKHSASSTRWLKEHFDDKYVNEAKKKGYRSRAIFKIEEIQNKDKLLKAGMTVVDLGAAPGGWSQFAAKVVGEGGRVI
ACDLLPMESIAGVSFLQGDFREEAVLNALLDRIQPDMVDVVMSDMAPNMAGNLSVDQPRAMYLVELALDMCRQVLAPNGS
FVVKVFQGEGFDDYVKAVRDLFKVVKIRKPDSSRSRSREVFIVATGYKG
>Mature_208_residues
SKQKHSASSTRWLKEHFDDKYVNEAKKKGYRSRAIFKIEEIQNKDKLLKAGMTVVDLGAAPGGWSQFAAKVVGEGGRVIA
CDLLPMESIAGVSFLQGDFREEAVLNALLDRIQPDMVDVVMSDMAPNMAGNLSVDQPRAMYLVELALDMCRQVLAPNGSF
VVKVFQGEGFDDYVKAVRDLFKVVKIRKPDSSRSRSREVFIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=206, Percent_Identity=33.9805825242718, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=35.1063829787234, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=70.1923076923077, Blast_Score=308, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=34.9206349206349, Blast_Score=116, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=34.9206349206349, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=34.9206349206349, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=33.5078534031414, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=30.9090909090909, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=34.0314136125654, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=33.8383838383838, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=97, Percent_Identity=38.1443298969072, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=37.2340425531915, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356387, Length=215, Percent_Identity=35.3488372093023, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=30.8080808080808, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=32.6424870466321, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_VIBC3 (A5F937)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F937
- SMR:   A5F937
- STRING:   A5F937
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0165
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   PRK11188
- BioCyc:   VCHO345073:VC0395_A0165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23197; Mature: 23066

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: ACT_SITE 164-164 BINDING 63-63 BINDING 65-65 BINDING 83-83 BINDING 99-99 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQKHSASSTRWLKEHFDDKYVNEAKKKGYRSRAIFKIEEIQNKDKLLKAGMTVVDLGA
CCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCEEECCC
APGGWSQFAAKVVGEGGRVIACDLLPMESIAGVSFLQGDFREEAVLNALLDRIQPDMVDV
CCCCHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VMSDMAPNMAGNLSVDQPRAMYLVELALDMCRQVLAPNGSFVVKVFQGEGFDDYVKAVRD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
LFKVVKIRKPDSSRSRSREVFIVATGYKG
HHHHHHCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKQKHSASSTRWLKEHFDDKYVNEAKKKGYRSRAIFKIEEIQNKDKLLKAGMTVVDLGA
CCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCEEECCC
APGGWSQFAAKVVGEGGRVIACDLLPMESIAGVSFLQGDFREEAVLNALLDRIQPDMVDV
CCCCHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VMSDMAPNMAGNLSVDQPRAMYLVELALDMCRQVLAPNGSFVVKVFQGEGFDDYVKAVRD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
LFKVVKIRKPDSSRSRSREVFIVATGYKG
HHHHHHCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA