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The map label for this gene is ptsP [H]

Identifier: 147673679

GI number: 147673679

Start: 208442

End: 210688

Strand: Direct

Name: ptsP [H]

Synonym: VC0395_A0204

Alternate gene names: 147673679

Gene position: 208442-210688 (Clockwise)

Preceding gene: 147674319

Following gene: 147675364

Centisome position: 6.89

GC content: 48.73

Gene sequence:

>2247_bases
ATGCTTTCTCAGCTAAGGGATATAGTAGAACAAGTTTCAAAAGTCGAAGACGTCTATCAGGCGTTGGATATTTTTGTAAA
GCAGACCTGTGAGGCGATGAGCACAGAGTGTTGTACTGTCTATTTAGCGAATGAAGAGATGCACCGTCTGGAATTGATGG
CCACACAAGGTCTGAAGTTCAAGGGCGATAAGATCCATATTGATTTTGATGAAGGCTTGGTCGGGTTGGTTAAACGTTCT
GCCGAACCGATTAACTTAGCCGAAGCCTCAAAACATCCGAGCTTTAAATATTTTAAGCAGTTGGGTGAAGAGGTTTATCA
CTCCTTCCTCGGTACACCGATCATCTATCGCAAACAGGTGCTTGGTGTGCTGGTGGTGCAGCAAAAATCTCCCCGTTTAT
TTAGCGAGATGGAAGTCTCCTTTTTAGTTACCCTTGCAGCGCAGCTCGCGGTGCTGGTGGCGCATGCTCAAACGCAAGGT
CACTGGCGTTTATCCAAAAAGCAGCAGGCCATTACGGGTGTTGCCGCCTCTTCTGGGGTCGCGATTGGTGAGTTTTGGTG
GGATGATACCCAGCCAGATCTGTCCGAAGTGCTACCCGCCTCAGCTCTGGATCCTGATTTTGAGCAAGAGCGTATTGCCT
TGGCAGTAGAGAGTGCGCTGGCCGATTTTCGTCGGATGCGTAAAAAGCTCGATGGGGATATCAACAAAGAAGCGTTAGCC
ATTTTTGATCTGTTCACTCACTTATTGAACGATCCTATGCTGCGGAAAGATCTCAAAGCGCAGATCCAAAAAGGGGATCG
TGCCGATTGGGCGCTGCGCCAAGTGGTCGAAAGTTACTCGAACCGTTTTGCGCGCATGTCGGATGTTTATCTGCGAGAGC
GAGCGCAAGACATTCGTGAGTTAGGACAACGTTTACTGTTTTTCTTGCTCAATACCGAAACCAATCAGCCCAAAATCGAT
AAGCCGGTTATCTTGGTGGTGCGAGAGCTGACCGCGAGCGTGTTAGCCGCACTGCCCAAAGATAAGCTGCTGGCTGTCGT
CTCTCTTGAAGGCGCGGCCAACTCCCATGCGGCTATTTTGTCGCGCGCGTTAGGCATTCCTGCGGTGATGGGGGTATCGA
TTAACCTGCGCGATCTGAATGGCAAAAAAGGTATTGTCGATGGCTACAGCGGTAAGTTATTCATTTCGCCTTCGAAAACC
ATTCTGAATGAGTATCGTGCCTTAGCGAATGAAGAGCGTGAGCTGTCGCGCATGGTCAATGAGGCGATTCGTGAGCCCGC
ATGTACTCTTGATGGCGCACGAATCGAATTGTTACTGAATGCCGGTTTGAGTGCCGATACCAGTATTGCCGTCAATCAAG
GGGTGGATGGCGTTGGGTTGTATCGCACAGAAATCTCTTTCTTACTGCAGCACCGATTTCCCTCAGAAGAGGAGCAGACT
CAGCAATATCGGCATGTGCTGAACACTTACCCTCATCAACGTGTTGTTATGCGTACGCTCGATATCGGTGGTGATAAGCC
TTTACCTTACTTGCCGATTGAAGAAGACAACCCATTTCTCGGTTGGCGTGGTATCCGTTTTACCTTAGATCATCCTGACA
TTTTTATTATCCAACTGCGCGCCATGCTCAGAGCAAGTGCGGAGTCGGGGAATTTATCCATCTTGCTGCCGATGATTTCC
GGTGCCAAAGAGCTGGATGATGCGCTGAAGTTTATCTATCAAGCCTATCAAGAAGTTTCGCAACAAGATCCACGCGTGGT
GATGCCGCAAATCGGGATCATGCTCGAAGTACCTTCGATGCTATATCTGCTGCCACTGATTGCCGATAAAATTGATTTCG
TCTCTGTCGGTACAAACGATTTGACCCAATATCTGCTTGCCGTGGATCGTAACAATGCGCGTGTGGCGGATGTGTATGAA
TCTATGCATCCTGCGGTGGTGATGGCACTGAAGCAGATCCAACAGACTTGTGCGACCCATCAAATACCCGTGTGTGTCTG
TGGTGAGTTAGCCGGAGATCCGATTGGTGCTTTGCTGTTGATAGGGTTAGGCTACACCACCTTAAGTATGAACACGTCTA
ACGTCGCTAAAGTGAAATATCTAGTGCGACACTCTGAACTGGCGGAATTAACCCAGCTCGCTGAGCAGGCATTAACCCAA
CCTTATGGACGAGAAATCTACAATATGATGCTCGCCTATATCGAAAAACACGGTTTTGCAGGTTTTGTCCGTGCAGGCAA
AAAATAG

Upstream 100 bases:

>100_bases
GTGATGTTTATCGCCGCGCAATGAAAGAGTTCGCCTCATTAGCGATGCCATTTAAAGAACGGAAAGTGAAGGGCAAACGC
AACACTCACAGAGGTTAAGC

Downstream 100 bases:

>100_bases
GAACCAAATGTGAATATTGAACTCTTAGCTATACTGCTGCTGTTAGGGGCAGTGGTGGGTGTGCTTGCTGGCTTATTGGG
CATTGGTGGCGGCTTACTAG

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKFKGDKIHIDFDEGLVGLVKRS
AEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQVLGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQG
HWRLSKKQQAITGVAASSGVAIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA
IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARMSDVYLRERAQDIRELGQRLLFFLLNTETNQPKID
KPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAILSRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKT
ILNEYRALANEERELSRMVNEAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT
QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASAESGNLSILLPMIS
GAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSMLYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYE
SMHPAVVMALKQIQQTCATHQIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ
PYGREIYNMMLAYIEKHGFAGFVRAGKK

Sequences:

>Translated_748_residues
MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKFKGDKIHIDFDEGLVGLVKRS
AEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQVLGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQG
HWRLSKKQQAITGVAASSGVAIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA
IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARMSDVYLRERAQDIRELGQRLLFFLLNTETNQPKID
KPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAILSRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKT
ILNEYRALANEERELSRMVNEAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT
QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASAESGNLSILLPMIS
GAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSMLYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYE
SMHPAVVMALKQIQQTCATHQIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ
PYGREIYNMMLAYIEKHGFAGFVRAGKK
>Mature_748_residues
MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKFKGDKIHIDFDEGLVGLVKRS
AEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQVLGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQG
HWRLSKKQQAITGVAASSGVAIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA
IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARMSDVYLRERAQDIRELGQRLLFFLLNTETNQPKID
KPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAILSRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKT
ILNEYRALANEERELSRMVNEAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT
QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASAESGNLSILLPMIS
GAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSMLYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYE
SMHPAVVMALKQIQQTCATHQIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ
PYGREIYNMMLAYIEKHGFAGFVRAGKK

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=751, Percent_Identity=49.9334221038615, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=581, Percent_Identity=35.9724612736661, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI48994992, Length=507, Percent_Identity=33.5305719921105, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788726, Length=617, Percent_Identity=30.1458670988655, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1787994, Length=399, Percent_Identity=23.0576441102757, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI226510935, Length=183, Percent_Identity=28.4153005464481, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83568; Mature: 83568

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE
KGDKIHIDFDEGLVGLVKRSAEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQV
CCCEEEEECCCCHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQGHWRLSKKQQAITGVAASSGV
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCC
AIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA
EEEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARMSDVYLRERAQDIRE
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFFLLNTETNQPKIDKPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAIL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
SRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKTILNEYRALANEERELSRMVN
HHHHCCCEEEECEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHH
EAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT
HHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHH
QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLR
HHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCEEHHHHH
AMLRASAESGNLSILLPMISGAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSM
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEHHHH
LYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYESMHPAVVMALKQIQQTCATH
HHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ
CCCCEEECCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PYGREIYNMMLAYIEKHGFAGFVRAGKK
HHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE
KGDKIHIDFDEGLVGLVKRSAEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQV
CCCEEEEECCCCHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQGHWRLSKKQQAITGVAASSGV
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCC
AIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA
EEEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARMSDVYLRERAQDIRE
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFFLLNTETNQPKIDKPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAIL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
SRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKTILNEYRALANEERELSRMVN
HHHHCCCEEEECEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHH
EAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT
HHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHH
QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLR
HHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCEEHHHHH
AMLRASAESGNLSILLPMISGAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSM
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEHHHH
LYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYESMHPAVVMALKQIQQTCATH
HHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ
CCCCEEECCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PYGREIYNMMLAYIEKHGFAGFVRAGKK
HHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]