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The map label for this gene is slt [H]

Identifier: 147673203

GI number: 147673203

Start: 239162

End: 241108

Strand: Direct

Name: slt [H]

Synonym: VC0395_A0230

Alternate gene names: 147673203

Gene position: 239162-241108 (Clockwise)

Preceding gene: 147674187

Following gene: 147673635

Centisome position: 7.91

GC content: 47.2

Gene sequence:

>1947_bases
ATGACGCGCCTGACGGTATTTAAGCCGCGTAGCCTTGTTTCGATCTTGATGTTCACCACGGTATGGGCAGTGGGCTCAGC
GAGCGCCAGTACTTTGGATTTGGAAGCGCAGAGAGCTCAATATGATAAAGCGCAGCGTTGGTTGGACGAAAAGAATGTCG
CTCAATACCAACGTATTCGTAAGCAGATCGATAGCTATCCCTTAACCCCTTATCTCGATTATCGCGCTTTTCTTATCGAT
CTCGGCAGTAAGCCGCCGATCGCAGTACGCAATTTTATCGATAGTCATAAAGAGTACCCTTTTTCAGCTCGGATCGCTGC
GCCCTATTTAGACGCTTTAGCGCGCAGCAAAAAATGGTCGGCATTGCTGCAATTTCAAACTCAACTGCCCAATGGCGAAA
CTTACCAATGCCATTACTACAACGCCAAACTGCAAACCGGAAAGCGTAATGAGGCGTTTGAAGGTGCGAAAAAGCTGTGG
CTTAACGGTGCCAGTATCGCCGATGCTTGTGATCCGTTATTTGCCGAGTGGGATCGCGTTGGGGGCTTGAGTGACGATTG
GGTTTTAAAACGGGCTTTACTCGCTTTTGAAGGCCGTAACCGTAACCTCATCGTCTATCTACAGAAGAAGCTGGATGGCA
AAAAATCACAAGCTAAAGCGCAAGGCATGCTTGAGCTATTTGATAAACCTGAGCGAGTGTTAGCTTACAGCCGTAAAGCA
TCGCAAGATCCGATTAATCAAAAGTTAGCAGAGTTGGCGTTACAAAAGTGGGCAAGATCAGAGCCTCAAGAAGCGCAAGC
TGTCTTCAATGATGTCGCGAAAGCCCAAGGCTGGAATCAAGAGCAGAAAGGGCGAGTAGCTCGTTTTATTGCAATCCGTT
TGATGGATACTGAAGAGGCTGCGATTGCTAAATGGCGTGATGAGGTGACTCGCACTAGCCAAGATGTGCGTTTGATTGAA
GCGCGTATTCGACTAGCACTGCGTGAGAATGATTGGCGTGGTTTGAGTCAATGGATTGCGGTGTTACCAGAGCAAGAGCG
TAAAACTCTGCGTTGGCAATATTGGCAAGGTCGCAGTGAAATCGCGCTGGGTAAGAAAAAAGAAGGTACCGAGCGCTTAA
AAGCTTTGCTTGGACAACGCAGTTTTTACAGCGTAGCGGCAGCCAAAATTTTGCAGCAGTCGGTCAATTATCCGACCAGT
ACAGTAACGCTGGATATGAAGCAGATTAAAGCTCATAAAAAAGCGCTGGCCAGAATTGATGAACTGATTGCCTTGGATAA
AGTGCCGGCGGCAAAAAGTGAATGGCGTTGGTTGCTTGATCGAGTTTCTCAGAAAGAAAAAGAGATGTTGGCAGCGTATG
CCGCAGATTCAGGTTGGTATCAGATGACCATTGCGGCGACCATTTCCGCCAGCCTCTGGGATAACAATCAACTGCGTTTT
CCTGTCGTACATCAGAACTTGTTTACCTTGCATGGTCGAAAAAATGGTGTAGATCCGATCACATTAATGTCTTTAGCCAG
ACAAGAAAGTGCGCTTAACCCAGATGCGCAATCACCTGTTGGCGCACGGGGCTTAATGCAGATTATGCCGGATACTGCGC
GCTACACGGCAAGGAAATACCAATTGAGCTACTCCAATCCTGACGAACTCTATCAAGTGGGTAAGAATATTGAGATCGGT
AGCCGTTATCTCAGCAGTTTGCTTGAGCGTTATGACCAGAACCGAATCTTGGCTTTTGCTGCCTACAATGCAGGACCAAG
TCGGGTCGATAGTTGGTTGAAACGTAGCCAAGGTAAGCTGGATGCGTATGGCTTTATTGAAGCAATCCCCTTTGCTGAAA
CCCGCGGTTATGTGCAGAACATTTTGATGTTTGAAACCTACTATCGGGATTTGATGGGCGTACAAGGTCGCTTCTTGAAT
GAGCATGAGTTAAATACAAAGTATTGA

Upstream 100 bases:

>100_bases
GTTGCATTATTTGCGTTAAGTTTACTTCACTTGCCTTTACATTTATGGCGAGATTACGCGTAATAATTTGTTATCACACT
GATTTTAAGGATGAGATCTC

Downstream 100 bases:

>100_bases
TTCCTCAACTTGCTGCACTACAATAAGCCGGTGTATCACTTAACGAGTACAACTATGGTACAGCAACCTTCTTTTAGTGA
GTGGCAGCAAGTGTTGGATC

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MTRLTVFKPRSLVSILMFTTVWAVGSASASTLDLEAQRAQYDKAQRWLDEKNVAQYQRIRKQIDSYPLTPYLDYRAFLID
LGSKPPIAVRNFIDSHKEYPFSARIAAPYLDALARSKKWSALLQFQTQLPNGETYQCHYYNAKLQTGKRNEAFEGAKKLW
LNGASIADACDPLFAEWDRVGGLSDDWVLKRALLAFEGRNRNLIVYLQKKLDGKKSQAKAQGMLELFDKPERVLAYSRKA
SQDPINQKLAELALQKWARSEPQEAQAVFNDVAKAQGWNQEQKGRVARFIAIRLMDTEEAAIAKWRDEVTRTSQDVRLIE
ARIRLALRENDWRGLSQWIAVLPEQERKTLRWQYWQGRSEIALGKKKEGTERLKALLGQRSFYSVAAAKILQQSVNYPTS
TVTLDMKQIKAHKKALARIDELIALDKVPAAKSEWRWLLDRVSQKEKEMLAAYAADSGWYQMTIAATISASLWDNNQLRF
PVVHQNLFTLHGRKNGVDPITLMSLARQESALNPDAQSPVGARGLMQIMPDTARYTARKYQLSYSNPDELYQVGKNIEIG
SRYLSSLLERYDQNRILAFAAYNAGPSRVDSWLKRSQGKLDAYGFIEAIPFAETRGYVQNILMFETYYRDLMGVQGRFLN
EHELNTKY

Sequences:

>Translated_648_residues
MTRLTVFKPRSLVSILMFTTVWAVGSASASTLDLEAQRAQYDKAQRWLDEKNVAQYQRIRKQIDSYPLTPYLDYRAFLID
LGSKPPIAVRNFIDSHKEYPFSARIAAPYLDALARSKKWSALLQFQTQLPNGETYQCHYYNAKLQTGKRNEAFEGAKKLW
LNGASIADACDPLFAEWDRVGGLSDDWVLKRALLAFEGRNRNLIVYLQKKLDGKKSQAKAQGMLELFDKPERVLAYSRKA
SQDPINQKLAELALQKWARSEPQEAQAVFNDVAKAQGWNQEQKGRVARFIAIRLMDTEEAAIAKWRDEVTRTSQDVRLIE
ARIRLALRENDWRGLSQWIAVLPEQERKTLRWQYWQGRSEIALGKKKEGTERLKALLGQRSFYSVAAAKILQQSVNYPTS
TVTLDMKQIKAHKKALARIDELIALDKVPAAKSEWRWLLDRVSQKEKEMLAAYAADSGWYQMTIAATISASLWDNNQLRF
PVVHQNLFTLHGRKNGVDPITLMSLARQESALNPDAQSPVGARGLMQIMPDTARYTARKYQLSYSNPDELYQVGKNIEIG
SRYLSSLLERYDQNRILAFAAYNAGPSRVDSWLKRSQGKLDAYGFIEAIPFAETRGYVQNILMFETYYRDLMGVQGRFLN
EHELNTKY
>Mature_647_residues
TRLTVFKPRSLVSILMFTTVWAVGSASASTLDLEAQRAQYDKAQRWLDEKNVAQYQRIRKQIDSYPLTPYLDYRAFLIDL
GSKPPIAVRNFIDSHKEYPFSARIAAPYLDALARSKKWSALLQFQTQLPNGETYQCHYYNAKLQTGKRNEAFEGAKKLWL
NGASIADACDPLFAEWDRVGGLSDDWVLKRALLAFEGRNRNLIVYLQKKLDGKKSQAKAQGMLELFDKPERVLAYSRKAS
QDPINQKLAELALQKWARSEPQEAQAVFNDVAKAQGWNQEQKGRVARFIAIRLMDTEEAAIAKWRDEVTRTSQDVRLIEA
RIRLALRENDWRGLSQWIAVLPEQERKTLRWQYWQGRSEIALGKKKEGTERLKALLGQRSFYSVAAAKILQQSVNYPTST
VTLDMKQIKAHKKALARIDELIALDKVPAAKSEWRWLLDRVSQKEKEMLAAYAADSGWYQMTIAATISASLWDNNQLRFP
VVHQNLFTLHGRKNGVDPITLMSLARQESALNPDAQSPVGARGLMQIMPDTARYTARKYQLSYSNPDELYQVGKNIEIGS
RYLSSLLERYDQNRILAFAAYNAGPSRVDSWLKRSQGKLDAYGFIEAIPFAETRGYVQNILMFETYYRDLMGVQGRFLNE
HELNTKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=643, Percent_Identity=34.9922239502333, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74314; Mature: 74182

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLTVFKPRSLVSILMFTTVWAVGSASASTLDLEAQRAQYDKAQRWLDEKNVAQYQRIR
CCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHH
KQIDSYPLTPYLDYRAFLIDLGSKPPIAVRNFIDSHKEYPFSARIAAPYLDALARSKKWS
HHHHCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHH
ALLQFQTQLPNGETYQCHYYNAKLQTGKRNEAFEGAKKLWLNGASIADACDPLFAEWDRV
HHHHHHHCCCCCCEEEEEEEECEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GGLSDDWVLKRALLAFEGRNRNLIVYLQKKLDGKKSQAKAQGMLELFDKPERVLAYSRKA
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
SQDPINQKLAELALQKWARSEPQEAQAVFNDVAKAQGWNQEQKGRVARFIAIRLMDTEEA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCHHEEEEEEEEECCHHH
AIAKWRDEVTRTSQDVRLIEARIRLALRENDWRGLSQWIAVLPEQERKTLRWQYWQGRSE
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHEHHHHHCCCCC
IALGKKKEGTERLKALLGQRSFYSVAAAKILQQSVNYPTSTVTLDMKQIKAHKKALARID
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
ELIALDKVPAAKSEWRWLLDRVSQKEKEMLAAYAADSGWYQMTIAATISASLWDNNQLRF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHCCCCCEEE
PVVHQNLFTLHGRKNGVDPITLMSLARQESALNPDAQSPVGARGLMQIMPDTARYTARKY
EEECCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHEE
QLSYSNPDELYQVGKNIEIGSRYLSSLLERYDQNRILAFAAYNAGPSRVDSWLKRSQGKL
EECCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCC
DAYGFIEAIPFAETRGYVQNILMFETYYRDLMGVQGRFLNEHELNTKY
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRLTVFKPRSLVSILMFTTVWAVGSASASTLDLEAQRAQYDKAQRWLDEKNVAQYQRIR
CCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHH
KQIDSYPLTPYLDYRAFLIDLGSKPPIAVRNFIDSHKEYPFSARIAAPYLDALARSKKWS
HHHHCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHH
ALLQFQTQLPNGETYQCHYYNAKLQTGKRNEAFEGAKKLWLNGASIADACDPLFAEWDRV
HHHHHHHCCCCCCEEEEEEEECEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GGLSDDWVLKRALLAFEGRNRNLIVYLQKKLDGKKSQAKAQGMLELFDKPERVLAYSRKA
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
SQDPINQKLAELALQKWARSEPQEAQAVFNDVAKAQGWNQEQKGRVARFIAIRLMDTEEA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCHHEEEEEEEEECCHHH
AIAKWRDEVTRTSQDVRLIEARIRLALRENDWRGLSQWIAVLPEQERKTLRWQYWQGRSE
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHEHHHHHCCCCC
IALGKKKEGTERLKALLGQRSFYSVAAAKILQQSVNYPTSTVTLDMKQIKAHKKALARID
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
ELIALDKVPAAKSEWRWLLDRVSQKEKEMLAAYAADSGWYQMTIAATISASLWDNNQLRF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHCCCCCEEE
PVVHQNLFTLHGRKNGVDPITLMSLARQESALNPDAQSPVGARGLMQIMPDTARYTARKY
EEECCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHEE
QLSYSNPDELYQVGKNIEIGSRYLSSLLERYDQNRILAFAAYNAGPSRVDSWLKRSQGKL
EECCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCC
DAYGFIEAIPFAETRGYVQNILMFETYYRDLMGVQGRFLNEHELNTKY
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]