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The map label for this gene is thrB

Identifier: 147673067

GI number: 147673067

Start: 2072106

End: 2073062

Strand: Reverse

Name: thrB

Synonym: VC0395_A1942

Alternate gene names: 147673067

Gene position: 2073062-2072106 (Counterclockwise)

Preceding gene: 229259765

Following gene: 147673667

Centisome position: 68.55

GC content: 52.14

Gene sequence:

>957_bases
ATGAGTGTTGTGGTGTACGCACCCGCTTCCATCGGTAATGTCAGTGTCGGATTTGATGTGTTGGGGGCCGCGGTGTCCCC
GATTGATGGCACGTTACTCGGCGATCGAGTGAAAGTGGAAGCTGGCGCAGAGGCTTTCACACTGAAAACGGCGGGGCGAT
TTGTCGATAAGCTTCCCGCTAATCCGCAAGAAAACATTGTGTATGACTGCTGGCAAGTATTCGCGCGTGAGCTAGAGAAA
AAGTCGGTGGTACTGAAGCCGCTGACCATGACGCTAGAAAAAAATATGCCGATTGGTTCAGGATTGGGCTCCAGTGCTTG
CTCGATTGTCGCGGCTCTGGATGCGTTGAATCAGTTTCACGCCAGTCCGCTTGATGAAACTGAGCTTCTGGCTCTGATGG
GGGAAATGGAAGGCAAGATCTCTGGCAGTATTCACTATGACAACGTTGCTCCCTGCTATTTAGGTGGCGTGCAGTTGATG
CTCGAAGAACTCGGTATCATTAGTCAATCCGTGCCGAGTTTTGATGACTGGTATTGGGTGATGGCCTATCCGGGGATCAA
GGTGTCCACGGCGGAAGCGCGTGCCATTTTGCCTGCGCAATATCGCCGCCAAGATATTGTGGCGCATGGCCGCTATCTGG
CAGGATTTATTCACGCTTGCCATACTCAGCAGCCTGAATTAGCGGCAAAAATGATCAAAGACGTGATTGCCGAACCCTAT
CGTGAGAAACTGCTGCCGGGTTTTGCCAAAGCGCGCAGCTACGCCGCTGCGGCTGGCGCACTGGCAACGGGCATTTCGGG
CAGTGGTCCGACTTTGTTTAGCGTGTGCAAAGAACAAGCAGTGGCTGAACGTGTGGCACGTTGGCTTGAGCAAAACTATG
TGCAAAATGAAGAAGGATTCGTCCACATTTGTCGTCTAGACAAGCAAGGTTCGAAAGTAACAGGAAGTGAGCTATGA

Upstream 100 bases:

>100_bases
ACGGCGCGGGTTCTGAAGTGACTGCTGCAGGCGTGTTCTCAGATGTGATGCGTACACTCGGTTGGAAATTAGGGGTTTAA
TAGAAATGAGTTCAGGTGAC

Downstream 100 bases:

>100_bases
AGCTGTACAACATAAAAGAAAATGATGAACAAGTCTCTTTTGGTCAAGCCGTTCGCCAAGGGTTAGGCCGCAATCAAGGC
CTATTTTTTCCGGCACAATT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSVVVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDRVKVEAGAEAFTLKTAGRFVDKLPANPQENIVYDCWQVFARELEK
KSVVLKPLTMTLEKNMPIGSGLGSSACSIVAALDALNQFHASPLDETELLALMGEMEGKISGSIHYDNVAPCYLGGVQLM
LEELGIISQSVPSFDDWYWVMAYPGIKVSTAEARAILPAQYRRQDIVAHGRYLAGFIHACHTQQPELAAKMIKDVIAEPY
REKLLPGFAKARSYAAAAGALATGISGSGPTLFSVCKEQAVAERVARWLEQNYVQNEEGFVHICRLDKQGSKVTGSEL

Sequences:

>Translated_318_residues
MSVVVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDRVKVEAGAEAFTLKTAGRFVDKLPANPQENIVYDCWQVFARELEK
KSVVLKPLTMTLEKNMPIGSGLGSSACSIVAALDALNQFHASPLDETELLALMGEMEGKISGSIHYDNVAPCYLGGVQLM
LEELGIISQSVPSFDDWYWVMAYPGIKVSTAEARAILPAQYRRQDIVAHGRYLAGFIHACHTQQPELAAKMIKDVIAEPY
REKLLPGFAKARSYAAAAGALATGISGSGPTLFSVCKEQAVAERVARWLEQNYVQNEEGFVHICRLDKQGSKVTGSEL
>Mature_317_residues
SVVVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDRVKVEAGAEAFTLKTAGRFVDKLPANPQENIVYDCWQVFARELEKK
SVVLKPLTMTLEKNMPIGSGLGSSACSIVAALDALNQFHASPLDETELLALMGEMEGKISGSIHYDNVAPCYLGGVQLML
EELGIISQSVPSFDDWYWVMAYPGIKVSTAEARAILPAQYRRQDIVAHGRYLAGFIHACHTQQPELAAKMIKDVIAEPYR
EKLLPGFAKARSYAAAAGALATGISGSGPTLFSVCKEQAVAERVARWLEQNYVQNEEGFVHICRLDKQGSKVTGSEL

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=311, Percent_Identity=70.7395498392283, Blast_Score=464, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_VIBC3 (A5F5R9)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F5R9
- SMR:   A5F5R9
- STRING:   A5F5R9
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1942
- eggNOG:   COG0083
- HOGENOM:   HBG646290
- OMA:   GSAHADN
- ProtClustDB:   PRK01212
- BioCyc:   VCHO345073:VC0395_A1942-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 34253; Mature: 34121

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDRVKVEAGAEAFTLKTAGRFVDKLPA
CEEEEECCCCCCCCEECHHHHHHHCCCCCCCEECCEEEEECCCCEEEEHHHHHHHHHCCC
NPQENIVYDCWQVFARELEKKSVVLKPLTMTLEKNMPIGSGLGSSACSIVAALDALNQFH
CCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
ASPLDETELLALMGEMEGKISGSIHYDNVAPCYLGGVQLMLEELGIISQSVPSFDDWYWV
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
MAYPGIKVSTAEARAILPAQYRRQDIVAHGRYLAGFIHACHTQQPELAAKMIKDVIAEPY
EECCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHH
REKLLPGFAKARSYAAAAGALATGISGSGPTLFSVCKEQAVAERVARWLEQNYVQNEEGF
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VHICRLDKQGSKVTGSEL
EEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SVVVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDRVKVEAGAEAFTLKTAGRFVDKLPA
EEEEECCCCCCCCEECHHHHHHHCCCCCCCEECCEEEEECCCCEEEEHHHHHHHHHCCC
NPQENIVYDCWQVFARELEKKSVVLKPLTMTLEKNMPIGSGLGSSACSIVAALDALNQFH
CCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
ASPLDETELLALMGEMEGKISGSIHYDNVAPCYLGGVQLMLEELGIISQSVPSFDDWYWV
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
MAYPGIKVSTAEARAILPAQYRRQDIVAHGRYLAGFIHACHTQQPELAAKMIKDVIAEPY
EECCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHH
REKLLPGFAKARSYAAAAGALATGISGSGPTLFSVCKEQAVAERVARWLEQNYVQNEEGF
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VHICRLDKQGSKVTGSEL
EEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA