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The map label for this gene is caiC [H]

Identifier: 146343881

GI number: 146343881

Start: 7444991

End: 7446565

Strand: Direct

Name: caiC [H]

Synonym: BRADO7132

Alternate gene names: 146343881

Gene position: 7444991-7446565 (Clockwise)

Preceding gene: 146343878

Following gene: 146343887

Centisome position: 99.84

GC content: 67.62

Gene sequence:

>1575_bases
ATGAACGGACTGACGATCGCGCCCGGTACGGTGACGCCGTTCGACGGCATGGACGTGCCGTGGCTGCTGAAGCTGCGCAG
CGAGGTCAGGCGTGACCATCCGTTCCTGATCTGGGCGCCGTTCGACGCGCCGGCGCGGCGATGGAGCTATGGCGAGTTTC
ACGACCGCGTCGGCGCGCTGGCCGCGGGCCTCGTCAAGCGCGGCATCAAGCCGGGCGACGCGGTGCTGATCCATCTCGAC
AATTGCATCGAGGCGATGCTGGCGTGGTTCGCCTGCGTCGAGCTCGGCGCACTCGCGGTCACCACCAACACCCGCTCGGC
TGCCGCCGAGATGAGCTACTTCGCCGATCACTGCCGCGCGGTCGCCGCGATCACCCAGCCGGCCTATGCCGAGATGATCT
CGGCGCATTGCCGCGGCCTCCGCTGGCTTGCCGTGATCTCGCATGATCCGGGGCAGCCGGACGCAACGGCGGTCGGCGTG
GGCGACCGGTTCGAGACGCTGTTTGCCGACAGCGCCGACCGGCCGCGGCGGGCCACCGATCCGCTGGCGCCGTGCAGCGT
GCAGTACACGTCGGGGACGACGTCGCGGCCGAAGGCTGTGCTGTGGACGCACGCCAATGCGCTGTGGGGCGCCAAGGTCA
ACGCCGTGCACGAGGACCTGCACCAGGGCGACGTGCACCAGACCTATCTGCCGCTGTTCCACACCAATGCGCTGGCCTAT
TCGATGCTGGCGACCCTCTGGGTCGGCGCCAGCTGCGTGATCCAGCCACGCTTCTCGGCGAGCCGGTTCTGGAGCGTCGC
GGTGGAGCATGGCTGCACCTGGACGTCGACGATCCCGTTCTGCATGAAGGCACTGCTCGACCAGGAGGTGCCGAAGGACC
ACAGATTCCGGCTGTGGGGCACAGCGGTGTCGGAGCCGACGGCGTTCGCGAAGTTCGGCATCAAGATCATCGGCTGGTGG
GGCATGACCGAGACCATCACCCACGGCATCGTCGGCGAGGTCGACCAGCCGAACACGCCGATGTCGATCGGCCGCGCCGC
GCCGGAATACACCATCCGCATCACCGATGATGACGGACGGCCGACGGCGGTCGGCGACACCGGCAATCTCGCCATCAAGG
GCGTGCCCGGCCTGTCGCTGTTTTCAGAGTATCTCTACAACGAGGAAGCGACCCGGACGAGCTTCGATGCCGACGGCTTC
TTCCTCACCGGCGACCGTGTCACGCGGCTTGACAACGGCTTCATCAAGTTCGGCGATCGCACCAAGGACATGCTGAAGGT
CGGCGGCGAGAACGTCGCAGCGTCCGAGATCGAGCAGGTGATCATCACCGTGCCCGGTGTGCGCGAGACCGCGGTCGTCG
CCAAGAAGCACCCGATGCTCGACGAGGTGCCGGTGGCGTTCGTGATCCCGCAGGCCGGCGTCGCGCGCGAGGAGCTGGCG
GAGAAAGTGCTGGCCGCCTGCCGCGACGGTCTCGCCGACTTCAAGGTGCCGCGCGAGGTGAGGCTGGTCGACGTGATGCC
GCGCTCGACGCTCGAGAAGGTCGCCAAGGCCGAATTGCGCAAGCTGCTCGACTGA

Upstream 100 bases:

>100_bases
TCAATCCGATTTGCTCGCGGCGACGGTGAGTGGCAAAAGGTGGATGAACGACAAACGAAGCGGCCGGGACGGGCTGCGGA
CAAGAACAATGGGAGCGAAC

Downstream 100 bases:

>100_bases
CGATCGCGCGGCTCACTGCATCGTCACGCGGACCGGCAGCGTCTTCGGTCCGTTCACGAAGGTGGCCTGCGTCATCTTGA
CCGGCCGTCGAGCGACAGCG

Product: crotonobetaine/carnitine-CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MNGLTIAPGTVTPFDGMDVPWLLKLRSEVRRDHPFLIWAPFDAPARRWSYGEFHDRVGALAAGLVKRGIKPGDAVLIHLD
NCIEAMLAWFACVELGALAVTTNTRSAAAEMSYFADHCRAVAAITQPAYAEMISAHCRGLRWLAVISHDPGQPDATAVGV
GDRFETLFADSADRPRRATDPLAPCSVQYTSGTTSRPKAVLWTHANALWGAKVNAVHEDLHQGDVHQTYLPLFHTNALAY
SMLATLWVGASCVIQPRFSASRFWSVAVEHGCTWTSTIPFCMKALLDQEVPKDHRFRLWGTAVSEPTAFAKFGIKIIGWW
GMTETITHGIVGEVDQPNTPMSIGRAAPEYTIRITDDDGRPTAVGDTGNLAIKGVPGLSLFSEYLYNEEATRTSFDADGF
FLTGDRVTRLDNGFIKFGDRTKDMLKVGGENVAASEIEQVIITVPGVRETAVVAKKHPMLDEVPVAFVIPQAGVAREELA
EKVLAACRDGLADFKVPREVRLVDVMPRSTLEKVAKAELRKLLD

Sequences:

>Translated_524_residues
MNGLTIAPGTVTPFDGMDVPWLLKLRSEVRRDHPFLIWAPFDAPARRWSYGEFHDRVGALAAGLVKRGIKPGDAVLIHLD
NCIEAMLAWFACVELGALAVTTNTRSAAAEMSYFADHCRAVAAITQPAYAEMISAHCRGLRWLAVISHDPGQPDATAVGV
GDRFETLFADSADRPRRATDPLAPCSVQYTSGTTSRPKAVLWTHANALWGAKVNAVHEDLHQGDVHQTYLPLFHTNALAY
SMLATLWVGASCVIQPRFSASRFWSVAVEHGCTWTSTIPFCMKALLDQEVPKDHRFRLWGTAVSEPTAFAKFGIKIIGWW
GMTETITHGIVGEVDQPNTPMSIGRAAPEYTIRITDDDGRPTAVGDTGNLAIKGVPGLSLFSEYLYNEEATRTSFDADGF
FLTGDRVTRLDNGFIKFGDRTKDMLKVGGENVAASEIEQVIITVPGVRETAVVAKKHPMLDEVPVAFVIPQAGVAREELA
EKVLAACRDGLADFKVPREVRLVDVMPRSTLEKVAKAELRKLLD
>Mature_524_residues
MNGLTIAPGTVTPFDGMDVPWLLKLRSEVRRDHPFLIWAPFDAPARRWSYGEFHDRVGALAAGLVKRGIKPGDAVLIHLD
NCIEAMLAWFACVELGALAVTTNTRSAAAEMSYFADHCRAVAAITQPAYAEMISAHCRGLRWLAVISHDPGQPDATAVGV
GDRFETLFADSADRPRRATDPLAPCSVQYTSGTTSRPKAVLWTHANALWGAKVNAVHEDLHQGDVHQTYLPLFHTNALAY
SMLATLWVGASCVIQPRFSASRFWSVAVEHGCTWTSTIPFCMKALLDQEVPKDHRFRLWGTAVSEPTAFAKFGIKIIGWW
GMTETITHGIVGEVDQPNTPMSIGRAAPEYTIRITDDDGRPTAVGDTGNLAIKGVPGLSLFSEYLYNEEATRTSFDADGF
FLTGDRVTRLDNGFIKFGDRTKDMLKVGGENVAASEIEQVIITVPGVRETAVVAKKHPMLDEVPVAFVIPQAGVAREELA
EKVLAACRDGLADFKVPREVRLVDVMPRSTLEKVAKAELRKLLD

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=529, Percent_Identity=24.1965973534972, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI38505220, Length=514, Percent_Identity=24.7081712062257, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI187761345, Length=468, Percent_Identity=26.4957264957265, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI187761343, Length=468, Percent_Identity=26.4957264957265, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI115511026, Length=548, Percent_Identity=24.2700729927007, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=25.1428571428571, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=22.8962818003914, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=22.8962818003914, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI58082049, Length=511, Percent_Identity=22.5048923679061, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI13375727, Length=551, Percent_Identity=24.1379310344828, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI40807357, Length=539, Percent_Identity=26.3450834879406, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=23.7547892720307, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI13236579, Length=427, Percent_Identity=25.2927400468384, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI13325057, Length=365, Percent_Identity=27.6712328767123, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI38524616, Length=466, Percent_Identity=26.1802575107296, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI62865631, Length=558, Percent_Identity=24.7311827956989, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI13325055, Length=558, Percent_Identity=24.7311827956989, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI8923896, Length=539, Percent_Identity=23.747680890538, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI253970489, Length=389, Percent_Identity=25.706940874036, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=32.0486815415822, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI145693145, Length=501, Percent_Identity=25.5489021956088, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=25.6410256410256, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788107, Length=509, Percent_Identity=26.3261296660118, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=24.5353159851301, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786529, Length=537, Percent_Identity=24.5810055865922, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=29.718875502008, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32563687, Length=515, Percent_Identity=27.1844660194175, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI115534440, Length=516, Percent_Identity=25, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17551278, Length=532, Percent_Identity=25.187969924812, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=576, Percent_Identity=24.3055555555556, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=576, Percent_Identity=24.3055555555556, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=24.2700729927007, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=519, Percent_Identity=24.4701348747592, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=25.3649635036496, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17557194, Length=522, Percent_Identity=23.7547892720307, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=511, Percent_Identity=22.8962818003914, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=491, Percent_Identity=24.0325865580448, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982997, Length=530, Percent_Identity=25.2830188679245, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=530, Percent_Identity=25.2830188679245, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=357, Percent_Identity=26.6106442577031, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=145, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=341, Percent_Identity=26.099706744868, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581924, Length=521, Percent_Identity=26.1036468330134, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281364798, Length=526, Percent_Identity=24.9049429657795, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24583465, Length=523, Percent_Identity=25.0478011472275, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24583463, Length=523, Percent_Identity=25.0478011472275, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI62472339, Length=541, Percent_Identity=25.8780036968577, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24667955, Length=541, Percent_Identity=25.8780036968577, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130353, Length=534, Percent_Identity=25.6554307116105, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573631, Length=535, Percent_Identity=25.981308411215, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45552801, Length=524, Percent_Identity=23.4732824427481, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45550496, Length=526, Percent_Identity=23.384030418251, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355181, Length=499, Percent_Identity=25.4509018036072, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=28.3068783068783, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18859661, Length=546, Percent_Identity=24.1758241758242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24656500, Length=353, Percent_Identity=26.3456090651558, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 57366; Mature: 57366

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLTIAPGTVTPFDGMDVPWLLKLRSEVRRDHPFLIWAPFDAPARRWSYGEFHDRVGAL
CCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHCCCCHHHHHHHHHH
AAGLVKRGIKPGDAVLIHLDNCIEAMLAWFACVELGALAVTTNTRSAAAEMSYFADHCRA
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHH
VAAITQPAYAEMISAHCRGLRWLAVISHDPGQPDATAVGVGDRFETLFADSADRPRRATD
HHHHCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCC
PLAPCSVQYTSGTTSRPKAVLWTHANALWGAKVNAVHEDLHQGDVHQTYLPLFHTNALAY
CCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SMLATLWVGASCVIQPRFSASRFWSVAVEHGCTWTSTIPFCMKALLDQEVPKDHRFRLWG
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
TAVSEPTAFAKFGIKIIGWWGMTETITHGIVGEVDQPNTPMSIGRAAPEYTIRITDDDGR
CCCCCCHHHHHCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCC
PTAVGDTGNLAIKGVPGLSLFSEYLYNEEATRTSFDADGFFLTGDRVTRLDNGFIKFGDR
CEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEECCCCEEEECCC
TKDMLKVGGENVAASEIEQVIITVPGVRETAVVAKKHPMLDEVPVAFVIPQAGVAREELA
HHHHHHHCCCCCCHHHHCEEEEECCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHH
EKVLAACRDGLADFKVPREVRLVDVMPRSTLEKVAKAELRKLLD
HHHHHHHHCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNGLTIAPGTVTPFDGMDVPWLLKLRSEVRRDHPFLIWAPFDAPARRWSYGEFHDRVGAL
CCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHCCCCHHHHHHHHHH
AAGLVKRGIKPGDAVLIHLDNCIEAMLAWFACVELGALAVTTNTRSAAAEMSYFADHCRA
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHH
VAAITQPAYAEMISAHCRGLRWLAVISHDPGQPDATAVGVGDRFETLFADSADRPRRATD
HHHHCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCC
PLAPCSVQYTSGTTSRPKAVLWTHANALWGAKVNAVHEDLHQGDVHQTYLPLFHTNALAY
CCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SMLATLWVGASCVIQPRFSASRFWSVAVEHGCTWTSTIPFCMKALLDQEVPKDHRFRLWG
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
TAVSEPTAFAKFGIKIIGWWGMTETITHGIVGEVDQPNTPMSIGRAAPEYTIRITDDDGR
CCCCCCHHHHHCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCC
PTAVGDTGNLAIKGVPGLSLFSEYLYNEEATRTSFDADGFFLTGDRVTRLDNGFIKFGDR
CEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEECCCCEEEECCC
TKDMLKVGGENVAASEIEQVIITVPGVRETAVVAKKHPMLDEVPVAFVIPQAGVAREELA
HHHHHHHCCCCCCHHHHCEEEEECCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHH
EKVLAACRDGLADFKVPREVRLVDVMPRSTLEKVAKAELRKLLD
HHHHHHHHCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA