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The map label for this gene is potB [H]

Identifier: 146343841

GI number: 146343841

Start: 7403763

End: 7404629

Strand: Reverse

Name: potB [H]

Synonym: BRADO7091

Alternate gene names: 146343841

Gene position: 7404629-7403763 (Counterclockwise)

Preceding gene: 146343842

Following gene: 146343840

Centisome position: 99.3

GC content: 67.59

Gene sequence:

>867_bases
ATGACCGACGCGGTGCTGTCAGCTCCGATCCTCAAGCCCGCACCGGACCGCGAGCGGCTCGCGGCCTTTGGCTTCGTCGC
GCCATCGCTGCTGATCATTGCGCTGGTGTTCGTGCTGCCGCTGGCGCTGCTGCTCGGCGTGAGCGTGCTCGATCCCAAGG
GCGGCCTCACGGCGGCGCATTACGTCAGGCTGCTCGGCACGCCCTATTATCTCGGCGTCATCTGGAACTCGCTGCGGCTC
GGCCTGCTGGTCACCGCGATCGCTTTCCTGATCTCCTATCCCGCCGCCTTCGCGCTGGCCCGCGCCCGCGGCCCGCTGCG
CTCGATCCTGCTCGCGACGCTATTCCTGCCGCTCGCGGCCTCCGTCATCGTCAAGGCGTTCGCCTGGACCATCCTGCTGC
GCAGCGACGGCCTGATCAACACGGTGCTGATCGCACTGGGGATCATCGAGGATCCGATCCGCATGATCTTCACCCAGACG
GCGCTGATCGTCGGCGCGGTCAACATCTTCCTGCCGTTCATGGTGCTGCCGATCTACGCCGTCGTGGCGCAGCTCGACAG
CCGCTATGCGGAAGCCGCGGCGACGCTCGGCGCCTCGCCGATCGTCGTGTTCTTGCGCGTCATCCTGCCGCTGACACTGC
CCGGCATCATCGCCGGCGTCGCGCTGGTGTTCTCGCTGTCGGTCTCCGCCTACGTCGTGCCGACGCTCTTGATCGGCGAG
AAATACCCGACGCTCGCCACCACCATCGCAAAGGCCTATCTGCTGGCGCGCGAGCCCGGCTTCGGCGCCGCCGCCGGCGT
CGTGCTGCTGGCGATTGCGGTGATCGTCGTGGCGCTCAGCACGATGCTGTCGCGGGAGCCGAAATGA

Upstream 100 bases:

>100_bases
AGCTGTCGGGGCGCGAGGCCGAGCTGCCGCGCGTCCATGACGAGGTCCGCTTCGCGTGGCCCGTCGCCGAGACATTCGCG
TTTGCCGCCGAGGCCGCGCC

Downstream 100 bases:

>100_bases
TGACGCGCACGGCCATTATCATCTGCTGGATGCTCGCGATCGGCCTCGCGCTGTTCCTGATCCTGCCGCTCGCCGTCATC
GCCGCGGCGTCGTTCTCGCC

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTDAVLSAPILKPAPDRERLAAFGFVAPSLLIIALVFVLPLALLLGVSVLDPKGGLTAAHYVRLLGTPYYLGVIWNSLRL
GLLVTAIAFLISYPAAFALARARGPLRSILLATLFLPLAASVIVKAFAWTILLRSDGLINTVLIALGIIEDPIRMIFTQT
ALIVGAVNIFLPFMVLPIYAVVAQLDSRYAEAAATLGASPIVVFLRVILPLTLPGIIAGVALVFSLSVSAYVVPTLLIGE
KYPTLATTIAKAYLLAREPGFGAAAGVVLLAIAVIVVALSTMLSREPK

Sequences:

>Translated_288_residues
MTDAVLSAPILKPAPDRERLAAFGFVAPSLLIIALVFVLPLALLLGVSVLDPKGGLTAAHYVRLLGTPYYLGVIWNSLRL
GLLVTAIAFLISYPAAFALARARGPLRSILLATLFLPLAASVIVKAFAWTILLRSDGLINTVLIALGIIEDPIRMIFTQT
ALIVGAVNIFLPFMVLPIYAVVAQLDSRYAEAAATLGASPIVVFLRVILPLTLPGIIAGVALVFSLSVSAYVVPTLLIGE
KYPTLATTIAKAYLLAREPGFGAAAGVVLLAIAVIVVALSTMLSREPK
>Mature_287_residues
TDAVLSAPILKPAPDRERLAAFGFVAPSLLIIALVFVLPLALLLGVSVLDPKGGLTAAHYVRLLGTPYYLGVIWNSLRLG
LLVTAIAFLISYPAAFALARARGPLRSILLATLFLPLAASVIVKAFAWTILLRSDGLINTVLIALGIIEDPIRMIFTQTA
LIVGAVNIFLPFMVLPIYAVVAQLDSRYAEAAATLGASPIVVFLRVILPLTLPGIIAGVALVFSLSVSAYVVPTLLIGEK
YPTLATTIAKAYLLAREPGFGAAAGVVLLAIAVIVVALSTMLSREPK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=237, Percent_Identity=36.7088607594937, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=33.6363636363636, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1787713, Length=189, Percent_Identity=33.8624338624339, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787368, Length=233, Percent_Identity=27.8969957081545, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30303; Mature: 30172

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVLSAPILKPAPDRERLAAFGFVAPSLLIIALVFVLPLALLLGVSVLDPKGGLTAAH
CCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHH
YVRLLGTPYYLGVIWNSLRLGLLVTAIAFLISYPAAFALARARGPLRSILLATLFLPLAA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVIVKAFAWTILLRSDGLINTVLIALGIIEDPIRMIFTQTALIVGAVNIFLPFMVLPIYA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAQLDSRYAEAAATLGASPIVVFLRVILPLTLPGIIAGVALVFSLSVSAYVVPTLLIGE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KYPTLATTIAKAYLLAREPGFGAAAGVVLLAIAVIVVALSTMLSREPK
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDAVLSAPILKPAPDRERLAAFGFVAPSLLIIALVFVLPLALLLGVSVLDPKGGLTAAH
CCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHH
YVRLLGTPYYLGVIWNSLRLGLLVTAIAFLISYPAAFALARARGPLRSILLATLFLPLAA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVIVKAFAWTILLRSDGLINTVLIALGIIEDPIRMIFTQTALIVGAVNIFLPFMVLPIYA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAQLDSRYAEAAATLGASPIVVFLRVILPLTLPGIIAGVALVFSLSVSAYVVPTLLIGE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KYPTLATTIAKAYLLAREPGFGAAAGVVLLAIAVIVVALSTMLSREPK
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]