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The map label for this gene is pcpB [H]

Identifier: 146343679

GI number: 146343679

Start: 7196610

End: 7198298

Strand: Direct

Name: pcpB [H]

Synonym: BRADO6922

Alternate gene names: 146343679

Gene position: 7196610-7198298 (Clockwise)

Preceding gene: 146343676

Following gene: 146343689

Centisome position: 96.51

GC content: 63.11

Gene sequence:

>1689_bases
ATGGACTTCGACACTGACGTTCTGGTGCTGGGCGCGGGACCCGTCGGGACGACGCTCGGCAACGAACTGGTCCGCCACGG
GATCAAGCCCCGCATCGTCGACAGGGCACCGTCCATCCGAGAAGTTTCGAAAGCGATGATCCTGCATGTGCGCACGCAGG
AGGTGCTGGACAAGGTCGGCGTCGCGTCCCGGCTCGTCAGCGAGGCGCAACCATTGACCGAAGTGGTCGTGCACGCCTAT
GGCAAGCACATCGGCTCCTGGGATCTCGACGACATCGACAGCGACTACAAGCATCCGCTCATCATCGGGCAAAACAAGAC
CCAACACGCGCTTCTCGACCTCTTGAACGCAGGCGGCGTCGACGTGCAGTGGAACACCGAGGCGATGTCGTTCGAGATGA
CGCCCGATGGCGTGACCACGACATTGAAGGTGACGGATCCTGTCACGCACAGCACACGTGAAGACATCGTTCGCTCCCGT
TACATCTGCGGTTGCGAAGGGTCCAACAGCCTCGTGCGTCGGTCGCTCAACTTCACGTTCGAGGGCGACCGTTACACCGG
CGAGCAGTTCATCCAGGCCGACTGCCGCATCAGGTGGGCGTTGCCGAAAGGCAGATCCTATCTGTTCCTGACCGCTGTCG
GTTACATGATGGTGATCGAGTTTCCGAACGACGTCGTCCGGATATTCATTTCGCTGCCCGACACTCCCAACGCGGCTGGA
GCGAAGGAAGCCGCAGCCCAGCTCGGTGCGGTCGAGTCCACGACCGAGGAGCCGACGCTCGAGGACATCCGCCACCATTT
GACCGAGCTCTCGGGCTTCGCGTGCGAGCTGTCTGATCCGATCTGGCTCGCCCGCTATCGGACGTCGCACCGCTATGCCA
ATCATTTCAGCCAGGGCCGGGCCTTCATCGCCGGCGACGCGGGACATGTGCATGTGCCGATCGGCGGCCAGGGCATGAAC
ACGGGCATCCAGGATGCGTTCAATCTCGGTTGGAAACTGGCCGGTGTCGCAAAGGGCGAACTGAAGCCGTCCGTGCTTGA
GAGTTATCACGCCGAGCGCCACCCCGTGGCGGAAAGCCTGATCAAGGGCACGAATCTCGCCTACACGGGGATCCTGCACC
CGAGTGAGGCGCGCCAGCACGCCGCCCGCCTGTTCGGCCCTTTCCTGATCCGCAACGAGCGGGTCCAGGACTTCATGCGC
AATACGCTCGAGGAGCTGAAGATCTTCTATCCCTCATCGCCGCTCAATCTCGATCTCGGTGGCGCGACGGGACCCAGACC
CGGCGAGCGCGTTCTCGATACCACGATGGTCCGGGCCTCGGACAAGAGCACGGTCACGATCAACCGTCTGACCCGCTCGC
CAGGCTGGACGCTGTTGATCTTCGGCGGCCGCGACGACATGGCCCTCGACGAGGCGTTCGCATTGTCTGCCGATGTGACC
GGCCGCTTCGGCAAGCTGATGACGCCCTATGTGATCGTCGCCGACAAGATCGCGCCCGAGAGCGCAGGCGGATCCGACCA
TATCTTGCTCGACACGCTGCATCTTGCGCATGAGCGCTACGGCGTCACGACGCCGGTGTTCTACCTCCTGCGACCCGATA
CCTATATCGGCGCACGCGGCCCATTGAGCGCGCGCGCCGGCCTGCTGGCGCACCTCGAGACCATCTTTGCCGAGGCAGGC
TCGAACTGA

Upstream 100 bases:

>100_bases
CGAGCGGACGGCCGCGCTCGGCCAGACGTCGCTCAGCCGGGCCACCGGGGACGTGACGACAGGACCGCGACGGCGATCGC
TGCCGAGAGGAGCCGAGAGC

Downstream 100 bases:

>100_bases
GGTCTCCAACCGCGATCCATGCGCGGTGATGCTGTCGCTGAAGTCGGCCCTCTTTCCGCCATCAAAACCCAGTCGCCGCC
GGCCGTGGCGGTGGGAGAGG

Product: putative monooxygenase, FAD binding (hydroxylase)

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MDFDTDVLVLGAGPVGTTLGNELVRHGIKPRIVDRAPSIREVSKAMILHVRTQEVLDKVGVASRLVSEAQPLTEVVVHAY
GKHIGSWDLDDIDSDYKHPLIIGQNKTQHALLDLLNAGGVDVQWNTEAMSFEMTPDGVTTTLKVTDPVTHSTREDIVRSR
YICGCEGSNSLVRRSLNFTFEGDRYTGEQFIQADCRIRWALPKGRSYLFLTAVGYMMVIEFPNDVVRIFISLPDTPNAAG
AKEAAAQLGAVESTTEEPTLEDIRHHLTELSGFACELSDPIWLARYRTSHRYANHFSQGRAFIAGDAGHVHVPIGGQGMN
TGIQDAFNLGWKLAGVAKGELKPSVLESYHAERHPVAESLIKGTNLAYTGILHPSEARQHAARLFGPFLIRNERVQDFMR
NTLEELKIFYPSSPLNLDLGGATGPRPGERVLDTTMVRASDKSTVTINRLTRSPGWTLLIFGGRDDMALDEAFALSADVT
GRFGKLMTPYVIVADKIAPESAGGSDHILLDTLHLAHERYGVTTPVFYLLRPDTYIGARGPLSARAGLLAHLETIFAEAG
SN

Sequences:

>Translated_562_residues
MDFDTDVLVLGAGPVGTTLGNELVRHGIKPRIVDRAPSIREVSKAMILHVRTQEVLDKVGVASRLVSEAQPLTEVVVHAY
GKHIGSWDLDDIDSDYKHPLIIGQNKTQHALLDLLNAGGVDVQWNTEAMSFEMTPDGVTTTLKVTDPVTHSTREDIVRSR
YICGCEGSNSLVRRSLNFTFEGDRYTGEQFIQADCRIRWALPKGRSYLFLTAVGYMMVIEFPNDVVRIFISLPDTPNAAG
AKEAAAQLGAVESTTEEPTLEDIRHHLTELSGFACELSDPIWLARYRTSHRYANHFSQGRAFIAGDAGHVHVPIGGQGMN
TGIQDAFNLGWKLAGVAKGELKPSVLESYHAERHPVAESLIKGTNLAYTGILHPSEARQHAARLFGPFLIRNERVQDFMR
NTLEELKIFYPSSPLNLDLGGATGPRPGERVLDTTMVRASDKSTVTINRLTRSPGWTLLIFGGRDDMALDEAFALSADVT
GRFGKLMTPYVIVADKIAPESAGGSDHILLDTLHLAHERYGVTTPVFYLLRPDTYIGARGPLSARAGLLAHLETIFAEAG
SN
>Mature_562_residues
MDFDTDVLVLGAGPVGTTLGNELVRHGIKPRIVDRAPSIREVSKAMILHVRTQEVLDKVGVASRLVSEAQPLTEVVVHAY
GKHIGSWDLDDIDSDYKHPLIIGQNKTQHALLDLLNAGGVDVQWNTEAMSFEMTPDGVTTTLKVTDPVTHSTREDIVRSR
YICGCEGSNSLVRRSLNFTFEGDRYTGEQFIQADCRIRWALPKGRSYLFLTAVGYMMVIEFPNDVVRIFISLPDTPNAAG
AKEAAAQLGAVESTTEEPTLEDIRHHLTELSGFACELSDPIWLARYRTSHRYANHFSQGRAFIAGDAGHVHVPIGGQGMN
TGIQDAFNLGWKLAGVAKGELKPSVLESYHAERHPVAESLIKGTNLAYTGILHPSEARQHAARLFGPFLIRNERVQDFMR
NTLEELKIFYPSSPLNLDLGGATGPRPGERVLDTTMVRASDKSTVTINRLTRSPGWTLLIFGGRDDMALDEAFALSADVT
GRFGKLMTPYVIVADKIAPESAGGSDHILLDTLHLAHERYGVTTPVFYLLRPDTYIGARGPLSARAGLLAHLETIFAEAG
SN

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=371, Percent_Identity=26.1455525606469, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 61600; Mature: 61600

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDTDVLVLGAGPVGTTLGNELVRHGIKPRIVDRAPSIREVSKAMILHVRTQEVLDKVG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHEEEHHHHHHHHHH
VASRLVSEAQPLTEVVVHAYGKHIGSWDLDDIDSDYKHPLIIGQNKTQHALLDLLNAGGV
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCE
DVQWNTEAMSFEMTPDGVTTTLKVTDPVTHSTREDIVRSRYICGCEGSNSLVRRSLNFTF
EEEECCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCEEECCCCCCHHHHHCCCEEE
EGDRYTGEQFIQADCRIRWALPKGRSYLFLTAVGYMMVIEFPNDVVRIFISLPDTPNAAG
CCCCCCCHHHHEECCEEEEECCCCCCEEEEEECCEEEEEECCCCEEEEEEECCCCCCCCC
AKEAAAQLGAVESTTEEPTLEDIRHHLTELSGFACELSDPIWLARYRTSHRYANHFSQGR
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCEEEEEHHHHHHHHHHHHCCC
AFIAGDAGHVHVPIGGQGMNTGIQDAFNLGWKLAGVAKGELKPSVLESYHAERHPVAESL
EEEEECCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCHHHHHH
IKGTNLAYTGILHPSEARQHAARLFGPFLIRNERVQDFMRNTLEELKIFYPSSPLNLDLG
HCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCEEEEC
GATGPRPGERVLDTTMVRASDKSTVTINRLTRSPGWTLLIFGGRDDMALDEAFALSADVT
CCCCCCCCHHHHHHHHEECCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHHEEECCCC
GRFGKLMTPYVIVADKIAPESAGGSDHILLDTLHLAHERYGVTTPVFYLLRPDTYIGARG
CCHHHHCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCCEEEEECCCCEECCCC
PLSARAGLLAHLETIFAEAGSN
CCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFDTDVLVLGAGPVGTTLGNELVRHGIKPRIVDRAPSIREVSKAMILHVRTQEVLDKVG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHEEEHHHHHHHHHH
VASRLVSEAQPLTEVVVHAYGKHIGSWDLDDIDSDYKHPLIIGQNKTQHALLDLLNAGGV
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCE
DVQWNTEAMSFEMTPDGVTTTLKVTDPVTHSTREDIVRSRYICGCEGSNSLVRRSLNFTF
EEEECCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCEEECCCCCCHHHHHCCCEEE
EGDRYTGEQFIQADCRIRWALPKGRSYLFLTAVGYMMVIEFPNDVVRIFISLPDTPNAAG
CCCCCCCHHHHEECCEEEEECCCCCCEEEEEECCEEEEEECCCCEEEEEEECCCCCCCCC
AKEAAAQLGAVESTTEEPTLEDIRHHLTELSGFACELSDPIWLARYRTSHRYANHFSQGR
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCEEEEEHHHHHHHHHHHHCCC
AFIAGDAGHVHVPIGGQGMNTGIQDAFNLGWKLAGVAKGELKPSVLESYHAERHPVAESL
EEEEECCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCHHHHHH
IKGTNLAYTGILHPSEARQHAARLFGPFLIRNERVQDFMRNTLEELKIFYPSSPLNLDLG
HCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCEEEEC
GATGPRPGERVLDTTMVRASDKSTVTINRLTRSPGWTLLIFGGRDDMALDEAFALSADVT
CCCCCCCCHHHHHHHHEECCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHHEEECCCC
GRFGKLMTPYVIVADKIAPESAGGSDHILLDTLHLAHERYGVTTPVFYLLRPDTYIGARG
CCHHHHCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCCEEEEECCCCEECCCC
PLSARAGLLAHLETIFAEAGSN
CCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]