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The map label for this gene is alkJ [H]

Identifier: 146343615

GI number: 146343615

Start: 7114798

End: 7116399

Strand: Direct

Name: alkJ [H]

Synonym: BRADO6853

Alternate gene names: 146343615

Gene position: 7114798-7116399 (Clockwise)

Preceding gene: 146343614

Following gene: 146343618

Centisome position: 95.42

GC content: 68.23

Gene sequence:

>1602_bases
ATGGCTGCGCGCGGGTATGACTACATCATCGTTGGCGCAGGCTCGGCGGGATGCGTGCTCGCCAACAGATTGTCGGCGGA
CCCGGCGTGCCGGGTGCTGCTGCTGGAAGCCGGCGGCTCCGACCGGAACTTCTGGCTGCGGATTCCGATCGGCTATTACA
GGGCGATCTACGACAAGCGCTTCTCGCGCCTGTTCGACACCGAGCCGAGCGAGCTCACGGCCGGCCGGGCGATCGTCTGG
CCGCGCGGCCGCGTGCTCGGCGGCTCGTCCTCGATCAACGGCCTGATCTTCATTCGCGGCGAGCCCGCCGGCTTCGACGA
CTGGGAACGCGCCGGCGCGCGTGGCTGGAGCTACAATCATCTGCTGCCGTTTTTCCGGCGCTACGAACGCTATGAGGGCG
GAGACAGCCAATATCACGGCGGCCTTGGCGAGTTTTCGGTGTCTGAGCTGCGCACAGGCAATCCCGCTTCGCAGGCCTGG
GTCGATGCCGCGGCCGAATTCGGGCTGCCGCGCAATCCCGATTTCAACGGCCCGACGACGCTGGGCGCCGGCAGCTATCA
GCTCGGTATCGGACGCCACTGGCGCTCCAGCGCAGCGACCGCGTTCCTCCACCCCATCATGAACCGCGCCAACCTGACGG
TCGTGACCGGCGTCCAGGTCAGCCGCGTGATATTGCGCGGATCGTCGGCGAGCGGCGTGGAATGGATCAAGGACGGCACC
ATCACATCCGCCATGGCCGATCGCGAGGTGCTGCTCGCGGCCGGCGCGCTGCAGTCGCCGCAGCTCTTGCAACTGTCGGG
GATCGGCCCAGCCGATCTGTTGCGCCGGCTCGGCATTCCCGTCGTCGTCGACGCGCCGGAAGTCGGCGAGAACCTGCAGG
ATCATTATCAAGCACGCATGATCGTGCGCCTGAAGCAGCCGATCTCGGTGAACGACCAGGTTCGCAGTCCCTATGAGCTC
GCCAAGATGGGGCTGCAATGGCTGTTCGCCGGCAGCGGTCCGCTGACCGCCGGGGCCGGCCAGGTCGGCGGCGCCGCCTG
CACCCATCTGGCCAGCGGCGGCCGCCCGGATGTGCAGTTCAACGTCATGCCGCTGTCGGTCGACAGGCCGGGAACGCCGC
TGCACAGCTATTCCGGCTTCACGGCGTCGGTCTGGCAATGTCATCCGCAGGCGCGCGGCCGGATCGCGATCCGCTCGACC
GACCCGTTCGAGCAGCCCACGATCGCGCCGAACTATTTCGCCGAAGAGGCCGACCGGAAGACCATCGTCGCGGGCCTCGA
GATGCTGCGCGACATCTTCAGACAGCCGTCGTTCCAGAAGCTGTGGGACATCGAGGTCGCGCCGGGCGCGGCGGTGGCGA
GCGCGCCCGGCCTGTGGGATTTCGCGCGCAGCACCGGCGGGACGGTGTTTCATCCGGTCGGCACCTGCCGCATGGGCGGC
GATGATCACGCGGTGCTCGATCCGGCGTTGCGCGTCCGCGGCGTGGACCGGCTGCGCGTCATCGACGCCTCGGTGATGCC
GCAGATCACCTCGGCCAACACCAACGCGACGAGCCTGATGATCGGTGAGCGCGGCGCGGCGCTGGTCGGCGGAGAACCGT
GA

Upstream 100 bases:

>100_bases
CCGCCGCGATGGACGCCAACTGTCGCCGGCGTCAAGATGCGGTTCGAGTGGCCACGATTGTCGTGCCAATCGGCTGCATT
GCGTTCCAGGGGGCCGGTCC

Downstream 100 bases:

>100_bases
GCGGAGGCGCGCTGAGCGCCCGCATCATCAGCGCCTCGGATAGCTCGCCAGCGCGTCGAGCTGCCGCTGCGCCTCGGGCG
ATCCCCATTCGCGGGCGCGC

Product: putative glucose-methanol-choline (GMC) oxidoreductase choline dehydrogenase (CHD)

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MAARGYDYIIVGAGSAGCVLANRLSADPACRVLLLEAGGSDRNFWLRIPIGYYRAIYDKRFSRLFDTEPSELTAGRAIVW
PRGRVLGGSSSINGLIFIRGEPAGFDDWERAGARGWSYNHLLPFFRRYERYEGGDSQYHGGLGEFSVSELRTGNPASQAW
VDAAAEFGLPRNPDFNGPTTLGAGSYQLGIGRHWRSSAATAFLHPIMNRANLTVVTGVQVSRVILRGSSASGVEWIKDGT
ITSAMADREVLLAAGALQSPQLLQLSGIGPADLLRRLGIPVVVDAPEVGENLQDHYQARMIVRLKQPISVNDQVRSPYEL
AKMGLQWLFAGSGPLTAGAGQVGGAACTHLASGGRPDVQFNVMPLSVDRPGTPLHSYSGFTASVWQCHPQARGRIAIRST
DPFEQPTIAPNYFAEEADRKTIVAGLEMLRDIFRQPSFQKLWDIEVAPGAAVASAPGLWDFARSTGGTVFHPVGTCRMGG
DDHAVLDPALRVRGVDRLRVIDASVMPQITSANTNATSLMIGERGAALVGGEP

Sequences:

>Translated_533_residues
MAARGYDYIIVGAGSAGCVLANRLSADPACRVLLLEAGGSDRNFWLRIPIGYYRAIYDKRFSRLFDTEPSELTAGRAIVW
PRGRVLGGSSSINGLIFIRGEPAGFDDWERAGARGWSYNHLLPFFRRYERYEGGDSQYHGGLGEFSVSELRTGNPASQAW
VDAAAEFGLPRNPDFNGPTTLGAGSYQLGIGRHWRSSAATAFLHPIMNRANLTVVTGVQVSRVILRGSSASGVEWIKDGT
ITSAMADREVLLAAGALQSPQLLQLSGIGPADLLRRLGIPVVVDAPEVGENLQDHYQARMIVRLKQPISVNDQVRSPYEL
AKMGLQWLFAGSGPLTAGAGQVGGAACTHLASGGRPDVQFNVMPLSVDRPGTPLHSYSGFTASVWQCHPQARGRIAIRST
DPFEQPTIAPNYFAEEADRKTIVAGLEMLRDIFRQPSFQKLWDIEVAPGAAVASAPGLWDFARSTGGTVFHPVGTCRMGG
DDHAVLDPALRVRGVDRLRVIDASVMPQITSANTNATSLMIGERGAALVGGEP
>Mature_532_residues
AARGYDYIIVGAGSAGCVLANRLSADPACRVLLLEAGGSDRNFWLRIPIGYYRAIYDKRFSRLFDTEPSELTAGRAIVWP
RGRVLGGSSSINGLIFIRGEPAGFDDWERAGARGWSYNHLLPFFRRYERYEGGDSQYHGGLGEFSVSELRTGNPASQAWV
DAAAEFGLPRNPDFNGPTTLGAGSYQLGIGRHWRSSAATAFLHPIMNRANLTVVTGVQVSRVILRGSSASGVEWIKDGTI
TSAMADREVLLAAGALQSPQLLQLSGIGPADLLRRLGIPVVVDAPEVGENLQDHYQARMIVRLKQPISVNDQVRSPYELA
KMGLQWLFAGSGPLTAGAGQVGGAACTHLASGGRPDVQFNVMPLSVDRPGTPLHSYSGFTASVWQCHPQARGRIAIRSTD
PFEQPTIAPNYFAEEADRKTIVAGLEMLRDIFRQPSFQKLWDIEVAPGAAVASAPGLWDFARSTGGTVFHPVGTCRMGGD
DHAVLDPALRVRGVDRLRVIDASVMPQITSANTNATSLMIGERGAALVGGEP

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=546, Percent_Identity=39.3772893772894, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1786503, Length=545, Percent_Identity=39.0825688073394, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17532301, Length=552, Percent_Identity=35.5072463768116, Blast_Score=344, Evalue=7e-95,
Organism=Drosophila melanogaster, GI24642042, Length=566, Percent_Identity=35.6890459363958, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI24642059, Length=574, Percent_Identity=34.6689895470383, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24650267, Length=564, Percent_Identity=34.3971631205674, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24642048, Length=566, Percent_Identity=34.8056537102473, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI45551458, Length=568, Percent_Identity=32.5704225352113, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI45549471, Length=569, Percent_Identity=32.8646748681898, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17137792, Length=551, Percent_Identity=33.7568058076225, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI18859995, Length=578, Percent_Identity=34.2560553633218, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24642055, Length=570, Percent_Identity=34.2105263157895, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24642035, Length=573, Percent_Identity=32.9842931937173, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642051, Length=567, Percent_Identity=32.6278659611993, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24642037, Length=578, Percent_Identity=33.7370242214533, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642039, Length=565, Percent_Identity=32.3893805309734, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI18859993, Length=574, Percent_Identity=32.5783972125435, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24642057, Length=582, Percent_Identity=29.0378006872852, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24645930, Length=580, Percent_Identity=26.2068965517241, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 57312; Mature: 57180

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARGYDYIIVGAGSAGCVLANRLSADPACRVLLLEAGGSDRNFWLRIPIGYYRAIYDKR
CCCCCCCEEEEECCCCCEEEEHHCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHH
FSRLFDTEPSELTAGRAIVWPRGRVLGGSSSINGLIFIRGEPAGFDDWERAGARGWSYNH
HHHHHCCCCCHHCCCEEEECCCCEEECCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHH
LLPFFRRYERYEGGDSQYHGGLGEFSVSELRTGNPASQAWVDAAAEFGLPRNPDFNGPTT
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
LGAGSYQLGIGRHWRSSAATAFLHPIMNRANLTVVTGVQVSRVILRGSSASGVEWIKDGT
CCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCCEEECCCC
ITSAMADREVLLAAGALQSPQLLQLSGIGPADLLRRLGIPVVVDAPEVGENLQDHYQARM
HHHHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHCCCCEEEECHHHHHHHHHHHHHEE
IVRLKQPISVNDQVRSPYELAKMGLQWLFAGSGPLTAGAGQVGGAACTHLASGGRPDVQF
EEEECCCCCCCHHHCCHHHHHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEE
NVMPLSVDRPGTPLHSYSGFTASVWQCHPQARGRIAIRSTDPFEQPTIAPNYFAEEADRK
EEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCH
TIVAGLEMLRDIFRQPSFQKLWDIEVAPGAAVASAPGLWDFARSTGGTVFHPVGTCRMGG
HHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCHHHHHCCCCEEEECCCEEEECC
DDHAVLDPALRVRGVDRLRVIDASVMPQITSANTNATSLMIGERGAALVGGEP
CCCHHCCHHHHHCCCCEEEEECHHHCCCEECCCCCCEEEEEECCCCEECCCCC
>Mature Secondary Structure 
AARGYDYIIVGAGSAGCVLANRLSADPACRVLLLEAGGSDRNFWLRIPIGYYRAIYDKR
CCCCCCEEEEECCCCCEEEEHHCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHH
FSRLFDTEPSELTAGRAIVWPRGRVLGGSSSINGLIFIRGEPAGFDDWERAGARGWSYNH
HHHHHCCCCCHHCCCEEEECCCCEEECCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHH
LLPFFRRYERYEGGDSQYHGGLGEFSVSELRTGNPASQAWVDAAAEFGLPRNPDFNGPTT
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
LGAGSYQLGIGRHWRSSAATAFLHPIMNRANLTVVTGVQVSRVILRGSSASGVEWIKDGT
CCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCCEEECCCC
ITSAMADREVLLAAGALQSPQLLQLSGIGPADLLRRLGIPVVVDAPEVGENLQDHYQARM
HHHHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHCCCCEEEECHHHHHHHHHHHHHEE
IVRLKQPISVNDQVRSPYELAKMGLQWLFAGSGPLTAGAGQVGGAACTHLASGGRPDVQF
EEEECCCCCCCHHHCCHHHHHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEE
NVMPLSVDRPGTPLHSYSGFTASVWQCHPQARGRIAIRSTDPFEQPTIAPNYFAEEADRK
EEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCH
TIVAGLEMLRDIFRQPSFQKLWDIEVAPGAAVASAPGLWDFARSTGGTVFHPVGTCRMGG
HHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCHHHHHCCCCEEEECCCEEEECC
DDHAVLDPALRVRGVDRLRVIDASVMPQITSANTNATSLMIGERGAALVGGEP
CCCHHCCHHHHHCCCCEEEEECHHHCCCEECCCCCCEEEEEECCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]