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The map label for this gene is yfkN [H]

Identifier: 146343613

GI number: 146343613

Start: 7111782

End: 7113530

Strand: Direct

Name: yfkN [H]

Synonym: BRADO6851

Alternate gene names: 146343613

Gene position: 7111782-7113530 (Clockwise)

Preceding gene: 146343612

Following gene: 146343614

Centisome position: 95.38

GC content: 66.95

Gene sequence:

>1749_bases
ATGATCCGGCGCCGGGATTTTCTGAGAGCTTCAGGAGCCGCGATGCTCGCCGCGCCGGCGCTGCTGCGCAACGCCCGCGC
CGCCGACACCGTCAGCCTCTACGATGTCGAGAAGTTCGGCAACGCCCGCATCCTGCACATCACAGATACGCATGCGCAGC
TACGGCCGGTGTATTTCCGCGAGCCCAGCGTCAATCTCGGCGTCGGCGAGATGTGGGGCAGGCCGCCGCATCTGGTCGAG
CGCGCGTTCCTCGATCGCTATGGCATCCGCCCCGACAGCGCCGAGGCCTATGCCTTCACCTCGTTCGACTTCGAGAAATA
TGCCGGCCGCTTCGGCAAGATGGGCGGCTTCGCGCATCTGAAGACGCTGATCGACAAGCTGCGCGCCGATGCCGGCGATC
ATCGCTCGCTGCTGCTCGACGGCGGCGACCTCTGGCAGGGCACGGGACTCGCCAATGCGATGCGTGGCGCCGACATGGTG
AGCGCGGCCAATCTGCTCGGCATCGACGCGATGACCGGGCATTGGGAATTCACCTATGGCGAGGAGGCGCTGCGCGCCAA
TCTCGCGCGCTTCAAGGGCGAGTTCCTGGCGCAGAACGTATTCCTCACCGAGGAGGCGGCGTTCAACGATGCCAAGGCGT
TCGACCGCGGCTCGGGCCGCGTGTTCAAGCCGGCCACGATCAAGGAGATCGGCGGCGCGCGCATCGCGGTGATCGGCCAG
GCCTTCCCGTACGTGCCGATCGCCCATCCGCGCCGCTTCACGCCCGACTGGACCTTCGGCATCCGCGAGGAGGAGCTGCA
GAAGCTGGTCGACGCCTTGCGCGGCGCCGACAAGGTCGATGCGGTGATCCTGCTGTCGCACAACGGCATGGATGTCGACC
TGAAGCTCGCGAGCCGCGTCACCGGCATCGACGTCATCCTCGGCGGCCATACCCATGACGCGATCCCGCAGCCTGTCGCG
GTCAGCAACGCCAAGGGCGCCACGCTGGTCACCAATGCCGGGTCGAACGGCAAATTCCTCGGCGTGCTCGATCTCGACGT
CGGCAAGGGCCGCGTCGCCGACGCGCGCTACCGGCTGCTGCCGGTGTATTCGGAGCTGCTGAAGCCGGATCCGGCGATGC
AGGCGCTGATCGATCAGACGCAGCAGCCCCAGGTGGCGGCCTGGAGCGAGAAGCTCGCGACCTCCGACCGGCTCTTGTAT
CGCCGCGGCAATTTCGAGGGCACGACCGACGACCTGATCTGCGCGGCGCTGCGCCGCGAGCTCGACGCCGAGCTCGCGCT
GACGCCCGGGTTCCGCTGGGGCCCGAGCGTGCTCGCCAACCAGTCGATCATGCTGGAAGACGTGCTGGCGCAGACCGCGA
TCACCTACCCGGAAACCTACGTGCAGCGGCTGACCGGCGCGGAGATCAAGGCCGTGCTCGAGGACGTCTGCGACAACCTC
TTCAACGCGGATCCCTATCTGCAGCAGGGCGGCGACATGGTGCGTACTGGCGGCTTCTCTTACCGCTGCACGCCGTCGGA
ACCGATCGGCCACCGCATCTCGGATCTCACGCGCGATGATGGCCGTCCCTTGGAGGCGAGCAAGGGCTACAAGGTCGCCG
GCTGGGCGTCGATGAACGCACAGGATGGCAAGCCGGTGTGGGACGTGGTCGCCACCCATCTGCGCGACGGCCAGCTGGTG
CTGGTGGGAACCAACAAGGTCACGCTGTCGGGAATCGACGGCAATCCCGGATACGCCTCGCAATCCTGA

Upstream 100 bases:

>100_bases
TCGGCGCAGGAGGCCGAAGCGCTCGCCAAGGCCTCGATCGTCCAGGCCACCAGCGAGAAGCAGCGCTGGAAGGACCTGGA
GATCCGCTAGGACGCGAACG

Downstream 100 bases:

>100_bases
ACCGAATGGCGCACCGCAACGACGCTGCTTCGGCGCCGCATGGATGACCGCGGCCCGACCGTGGTCTAAACCGGCCTCGA
TCCGAATCGGGAGGAAGCGC

Product: putative 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MIRRRDFLRASGAAMLAAPALLRNARAADTVSLYDVEKFGNARILHITDTHAQLRPVYFREPSVNLGVGEMWGRPPHLVE
RAFLDRYGIRPDSAEAYAFTSFDFEKYAGRFGKMGGFAHLKTLIDKLRADAGDHRSLLLDGGDLWQGTGLANAMRGADMV
SAANLLGIDAMTGHWEFTYGEEALRANLARFKGEFLAQNVFLTEEAAFNDAKAFDRGSGRVFKPATIKEIGGARIAVIGQ
AFPYVPIAHPRRFTPDWTFGIREEELQKLVDALRGADKVDAVILLSHNGMDVDLKLASRVTGIDVILGGHTHDAIPQPVA
VSNAKGATLVTNAGSNGKFLGVLDLDVGKGRVADARYRLLPVYSELLKPDPAMQALIDQTQQPQVAAWSEKLATSDRLLY
RRGNFEGTTDDLICAALRRELDAELALTPGFRWGPSVLANQSIMLEDVLAQTAITYPETYVQRLTGAEIKAVLEDVCDNL
FNADPYLQQGGDMVRTGGFSYRCTPSEPIGHRISDLTRDDGRPLEASKGYKVAGWASMNAQDGKPVWDVVATHLRDGQLV
LVGTNKVTLSGIDGNPGYASQS

Sequences:

>Translated_582_residues
MIRRRDFLRASGAAMLAAPALLRNARAADTVSLYDVEKFGNARILHITDTHAQLRPVYFREPSVNLGVGEMWGRPPHLVE
RAFLDRYGIRPDSAEAYAFTSFDFEKYAGRFGKMGGFAHLKTLIDKLRADAGDHRSLLLDGGDLWQGTGLANAMRGADMV
SAANLLGIDAMTGHWEFTYGEEALRANLARFKGEFLAQNVFLTEEAAFNDAKAFDRGSGRVFKPATIKEIGGARIAVIGQ
AFPYVPIAHPRRFTPDWTFGIREEELQKLVDALRGADKVDAVILLSHNGMDVDLKLASRVTGIDVILGGHTHDAIPQPVA
VSNAKGATLVTNAGSNGKFLGVLDLDVGKGRVADARYRLLPVYSELLKPDPAMQALIDQTQQPQVAAWSEKLATSDRLLY
RRGNFEGTTDDLICAALRRELDAELALTPGFRWGPSVLANQSIMLEDVLAQTAITYPETYVQRLTGAEIKAVLEDVCDNL
FNADPYLQQGGDMVRTGGFSYRCTPSEPIGHRISDLTRDDGRPLEASKGYKVAGWASMNAQDGKPVWDVVATHLRDGQLV
LVGTNKVTLSGIDGNPGYASQS
>Mature_582_residues
MIRRRDFLRASGAAMLAAPALLRNARAADTVSLYDVEKFGNARILHITDTHAQLRPVYFREPSVNLGVGEMWGRPPHLVE
RAFLDRYGIRPDSAEAYAFTSFDFEKYAGRFGKMGGFAHLKTLIDKLRADAGDHRSLLLDGGDLWQGTGLANAMRGADMV
SAANLLGIDAMTGHWEFTYGEEALRANLARFKGEFLAQNVFLTEEAAFNDAKAFDRGSGRVFKPATIKEIGGARIAVIGQ
AFPYVPIAHPRRFTPDWTFGIREEELQKLVDALRGADKVDAVILLSHNGMDVDLKLASRVTGIDVILGGHTHDAIPQPVA
VSNAKGATLVTNAGSNGKFLGVLDLDVGKGRVADARYRLLPVYSELLKPDPAMQALIDQTQQPQVAAWSEKLATSDRLLY
RRGNFEGTTDDLICAALRRELDAELALTPGFRWGPSVLANQSIMLEDVLAQTAITYPETYVQRLTGAEIKAVLEDVCDNL
FNADPYLQQGGDMVRTGGFSYRCTPSEPIGHRISDLTRDDGRPLEASKGYKVAGWASMNAQDGKPVWDVVATHLRDGQLV
LVGTNKVTLSGIDGNPGYASQS

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1786687, Length=277, Percent_Identity=25.6317689530686, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 63490; Mature: 63490

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRRDFLRASGAAMLAAPALLRNARAADTVSLYDVEKFGNARILHITDTHAQLRPVYFR
CCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEHHHCCCEEEEEEECCCCCCCEEEEE
EPSVNLGVGEMWGRPPHLVERAFLDRYGIRPDSAEAYAFTSFDFEKYAGRFGKMGGFAHL
CCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHH
KTLIDKLRADAGDHRSLLLDGGDLWQGTGLANAMRGADMVSAANLLGIDAMTGHWEFTYG
HHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCEEEECC
EEALRANLARFKGEFLAQNVFLTEEAAFNDAKAFDRGSGRVFKPATIKEIGGARIAVIGQ
HHHHHHHHHHHHHHHHHCEEEEECHHCCCCHHHHCCCCCCEECCCHHHHCCCCEEEEEEC
AFPYVPIAHPRRFTPDWTFGIREEELQKLVDALRGADKVDAVILLSHNGMDVDLKLASRV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEHHCCC
TGIDVILGGHTHDAIPQPVAVSNAKGATLVTNAGSNGKFLGVLDLDVGKGRVADARYRLL
CCEEEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHH
PVYSELLKPDPAMQALIDQTQQPQVAAWSEKLATSDRLLYRRGNFEGTTDDLICAALRRE
HHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHH
LDAELALTPGFRWGPSVLANQSIMLEDVLAQTAITYPETYVQRLTGAEIKAVLEDVCDNL
CCCCEEECCCCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHH
FNADPYLQQGGDMVRTGGFSYRCTPSEPIGHRISDLTRDDGRPLEASKGYKVAGWASMNA
CCCCCHHHCCCCEEEECCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
QDGKPVWDVVATHLRDGQLVLVGTNKVTLSGIDGNPGYASQS
CCCCHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIRRRDFLRASGAAMLAAPALLRNARAADTVSLYDVEKFGNARILHITDTHAQLRPVYFR
CCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEHHHCCCEEEEEEECCCCCCCEEEEE
EPSVNLGVGEMWGRPPHLVERAFLDRYGIRPDSAEAYAFTSFDFEKYAGRFGKMGGFAHL
CCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHH
KTLIDKLRADAGDHRSLLLDGGDLWQGTGLANAMRGADMVSAANLLGIDAMTGHWEFTYG
HHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCEEEECC
EEALRANLARFKGEFLAQNVFLTEEAAFNDAKAFDRGSGRVFKPATIKEIGGARIAVIGQ
HHHHHHHHHHHHHHHHHCEEEEECHHCCCCHHHHCCCCCCEECCCHHHHCCCCEEEEEEC
AFPYVPIAHPRRFTPDWTFGIREEELQKLVDALRGADKVDAVILLSHNGMDVDLKLASRV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEHHCCC
TGIDVILGGHTHDAIPQPVAVSNAKGATLVTNAGSNGKFLGVLDLDVGKGRVADARYRLL
CCEEEEECCCCCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHH
PVYSELLKPDPAMQALIDQTQQPQVAAWSEKLATSDRLLYRRGNFEGTTDDLICAALRRE
HHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHH
LDAELALTPGFRWGPSVLANQSIMLEDVLAQTAITYPETYVQRLTGAEIKAVLEDVCDNL
CCCCEEECCCCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHH
FNADPYLQQGGDMVRTGGFSYRCTPSEPIGHRISDLTRDDGRPLEASKGYKVAGWASMNA
CCCCCHHHCCCCEEEECCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
QDGKPVWDVVATHLRDGQLVLVGTNKVTLSGIDGNPGYASQS
CCCCHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]