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The map label for this gene is gntR [H]

Identifier: 146343520

GI number: 146343520

Start: 7013528

End: 7014484

Strand: Direct

Name: gntR [H]

Synonym: BRADO6755

Alternate gene names: 146343520

Gene position: 7013528-7014484 (Clockwise)

Preceding gene: 146343519

Following gene: 146343521

Centisome position: 94.06

GC content: 68.23

Gene sequence:

>957_bases
GTGTCGCGCGCGCTCAAAAGCGATGGCGCCGTCTCCGAGGAGACGCGGCGCAGGATCCTCGCCGCTGTCGACGAGCTCGG
CTACGTGCTCGACCTCTCCGCCGGCGCGCTGTCGTCGAAACGCACCGGCTTTGTCGCCGTGCTGGTGCCCTCGATCAACA
ATTCCAACTTCTCCGACACCGCCCGCGGCATCACCGAGGTCATCGAGGGCCAGGGCCTGCAGATGCTGCTCGGCTACACC
GACTACGACATGGAGAAGGAGGAGCAACTGGTCCAGGCGATGCTGACGCGCCGCCCCGACGGCATCATCGTCACCGACGG
GCGGCACACGCCGAAGGCGCGGCGCATGCTGCAATCCGCCGGAATTCCCGTCGTCGAAACCTGGGATTTGCCAGCGGATC
CAATCGATCATGTGGTCGGCTTCTCCAATGCCGAGGCGTCGCGCGCACTCGTCCACCGGCTCTATCAGAAGGGCTATCGC
AACATCGGCTTCATCGGCGGCGCCAGCCGGCGCGACACCCGCGGCGCCGACCGCAGGCTCGGCTACACCCGCGCGGTCGA
GGAGCTCGGGCTCCCGGTCGGCCGCACCATCTCGTTCGGCAACCCGCCGATCTCGATGGAGCAAGGCGGCCAGGCCATCG
TGCATCTGGTGCAGCAATGGCCCGATGTCGACGCCGCCGTCTGCGTCTCCGACCTCTCCGCCTTCGGCGCGATCATGGAG
TGCCATCGCCGCGGCTGGACGGTGCCCGGCCGGATCGCGATCGCCGGCTTCGGCGATTTCGAGGTGTCGCGCTGCGCCTG
GCCGCGCATCACCACCGTCACTGTGGATTGCCGCAACATCGGCCGCGAGGCCGGCCGCGTGCTTCTGAATGCGCTCGACG
ACGTCCAGCACGACCGCACGCCGGCGCCCTCGACCCTGATCGTGCCGTTCGAGGTCATCGAGCGCGAAAGCACCTGA

Upstream 100 bases:

>100_bases
GAATGAGATGAAGCCCACACGCCCGACCACCCGACCGATCCCGAAGCGGGCCGCAACCATGGCGGAGGTGGCGGCGCGGG
CGGGCGTGTCGGCGATGACG

Downstream 100 bases:

>100_bases
TCCGCATGGCTTGACAGGGTCTTGGGGACCGCTACGATGTTATCGATAACAAAAATCGATCAGGGCTGGGCGGCGGTCGG
GGTGTCCTCGCCGTCAGCGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSRALKSDGAVSEETRRRILAAVDELGYVLDLSAGALSSKRTGFVAVLVPSINNSNFSDTARGITEVIEGQGLQMLLGYT
DYDMEKEEQLVQAMLTRRPDGIIVTDGRHTPKARRMLQSAGIPVVETWDLPADPIDHVVGFSNAEASRALVHRLYQKGYR
NIGFIGGASRRDTRGADRRLGYTRAVEELGLPVGRTISFGNPPISMEQGGQAIVHLVQQWPDVDAAVCVSDLSAFGAIME
CHRRGWTVPGRIAIAGFGDFEVSRCAWPRITTVTVDCRNIGREAGRVLLNALDDVQHDRTPAPSTLIVPFEVIEREST

Sequences:

>Translated_318_residues
MSRALKSDGAVSEETRRRILAAVDELGYVLDLSAGALSSKRTGFVAVLVPSINNSNFSDTARGITEVIEGQGLQMLLGYT
DYDMEKEEQLVQAMLTRRPDGIIVTDGRHTPKARRMLQSAGIPVVETWDLPADPIDHVVGFSNAEASRALVHRLYQKGYR
NIGFIGGASRRDTRGADRRLGYTRAVEELGLPVGRTISFGNPPISMEQGGQAIVHLVQQWPDVDAAVCVSDLSAFGAIME
CHRRGWTVPGRIAIAGFGDFEVSRCAWPRITTVTVDCRNIGREAGRVLLNALDDVQHDRTPAPSTLIVPFEVIEREST
>Mature_317_residues
SRALKSDGAVSEETRRRILAAVDELGYVLDLSAGALSSKRTGFVAVLVPSINNSNFSDTARGITEVIEGQGLQMLLGYTD
YDMEKEEQLVQAMLTRRPDGIIVTDGRHTPKARRMLQSAGIPVVETWDLPADPIDHVVGFSNAEASRALVHRLYQKGYRN
IGFIGGASRRDTRGADRRLGYTRAVEELGLPVGRTISFGNPPISMEQGGQAIVHLVQQWPDVDAAVCVSDLSAFGAIMEC
HRRGWTVPGRIAIAGFGDFEVSRCAWPRITTVTVDCRNIGREAGRVLLNALDDVQHDRTPAPSTLIVPFEVIEREST

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=290, Percent_Identity=33.7931034482759, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1790715, Length=303, Percent_Identity=32.3432343234323, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790369, Length=293, Percent_Identity=27.6450511945392, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787906, Length=323, Percent_Identity=27.2445820433437, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787948, Length=322, Percent_Identity=27.3291925465839, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789202, Length=278, Percent_Identity=26.978417266187, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=26.0726072607261, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788474, Length=291, Percent_Identity=24.0549828178694, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790194, Length=294, Percent_Identity=24.1496598639456, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34755; Mature: 34624

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRALKSDGAVSEETRRRILAAVDELGYVLDLSAGALSSKRTGFVAVLVPSINNSNFSDT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEEECCCCCCCCHH
ARGITEVIEGQGLQMLLGYTDYDMEKEEQLVQAMLTRRPDGIIVTDGRHTPKARRMLQSA
HHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHC
GIPVVETWDLPADPIDHVVGFSNAEASRALVHRLYQKGYRNIGFIGGASRRDTRGADRRL
CCCEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHH
GYTRAVEELGLPVGRTISFGNPPISMEQGGQAIVHLVQQWPDVDAAVCVSDLSAFGAIME
HHHHHHHHHCCCCCCEEECCCCCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
CHRRGWTVPGRIAIAGFGDFEVSRCAWPRITTVTVDCRNIGREAGRVLLNALDDVQHDRT
HHHCCCCCCCEEEEEECCCCCHHHCCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHCCCC
PAPSTLIVPFEVIEREST
CCCCEEEEEHHHHCCCCC
>Mature Secondary Structure 
SRALKSDGAVSEETRRRILAAVDELGYVLDLSAGALSSKRTGFVAVLVPSINNSNFSDT
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEEECCCCCCCCHH
ARGITEVIEGQGLQMLLGYTDYDMEKEEQLVQAMLTRRPDGIIVTDGRHTPKARRMLQSA
HHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHC
GIPVVETWDLPADPIDHVVGFSNAEASRALVHRLYQKGYRNIGFIGGASRRDTRGADRRL
CCCEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHH
GYTRAVEELGLPVGRTISFGNPPISMEQGGQAIVHLVQQWPDVDAAVCVSDLSAFGAIME
HHHHHHHHHCCCCCCEEECCCCCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
CHRRGWTVPGRIAIAGFGDFEVSRCAWPRITTVTVDCRNIGREAGRVLLNALDDVQHDRT
HHHCCCCCCCEEEEEECCCCCHHHCCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHCCCC
PAPSTLIVPFEVIEREST
CCCCEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]