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The map label for this gene is prpE [C]

Identifier: 146343365

GI number: 146343365

Start: 6841910

End: 6843814

Strand: Reverse

Name: prpE [C]

Synonym: BRADO6593

Alternate gene names: 146343365

Gene position: 6843814-6841910 (Counterclockwise)

Preceding gene: 146343366

Following gene: 146343364

Centisome position: 91.78

GC content: 66.19

Gene sequence:

>1905_bases
ATGAGCGCTGCGAGCCGCTATCACGAGGTCCATGCCCGGTCGCTGCAGGATCCGGAAGGGTTCTGGGCCGAGGCCGCCCG
CGCGATCGACTGGATCGAGCCGGCCAAGACAATCTTCGATCCCTCGATGGGCGCCTATGGCCGCTGGTTCGCCGGCGCCG
TCGTCAACACCTGCTTCAACGCGCTCGACCGCCACGTCGCCGCCGGCCGCGGCGAGCAGCTGGCTCTGATTCATGATTCG
CCGCTGACGAATTCCGTCACCAGGATCACCTATGCCGAGATGCTGAAGGAGGTACAGACGCTCGCCGCCATCATGCAGGA
TTTCGGCGTCGGCAAGGGTGACCGCGTCATCCTCTACATGCCGATGGTGCCGGAGGCGATGGTCGCGATGCTCGCCTGCG
CGCGCATCGGCGCGGTGCATTCAGTGGTGTTCGGCGGCTTCGCGGCCAAGGAGCTCGCGACCCGCATCGACGACGCGACC
CCGAAGCTGGTGCTGTCGGCGAGCTGCGGCATCGAGCCCGGCCGCATCGTGCAATACAAGCCGCTGCTCGACCAGGCGAT
CGAGCTTGCCGACAAGCGGCCGCAGGCCTGCATCATCCTGCAGCGGCCGGAGCATGTCTGCGAGCTCAAGCCCGGCCGCG
ACTACGACTGGGCGGCGCTGCGCAAATCCGCAATCGCCAACGGCAAGCTCGCGCCTTGCGTGCCCGTGCTCGCGACCGAT
CCGCTCTACATCCTCTACACCTCCGGCACCACCGGCAAGCCGAAGGGCGTCGTGCGCGACAATGGCGGTCACCTCGTCGC
GCTGAAATGGTCGATGGAGAATCTCTACGGCATCAAGCCCGGCGAGGTCTGGTGGTGCGGCTCCGACATCGGCTGGGTCG
TGGGCCACAGCTACATCATCTACGGGCCGCTGATTCACGGCGCGACCTCGGTGATGTATGAGGGCAAGCCGGTCGGCACG
CCCGATGCCGGCGCGTTCTGGCGCGTCATCGCCGACCACAAGGCCGTCGCGCTGTTCACCGCGCCCACGGCGTTCCGCGC
GATCCGCAAGGAAGACCCGGAAGGGACGTTGCTGAGCAAGTATGATCTGTCGCAGTTCCGCACGCTGTTCCTGGCCGGGG
AGCGCGCCGACCCGCCGACGGTGGAATGGGCCGAGCAGCAGCTCAAGGTGCCGGTGATCGATCATTGGTGGCAGACCGAG
ACCGGCTGGTGCATCGCCGGCAACCCGGTGGGCCTCGGCCTCTTACCGGTCAAGCATGGGTCTCCGACGGTGCCGATGCC
GGGCTATCAGGTCGATATCGTCGACGAGGCAGCGAAGCCGGTGCCTGCCAACACAATGGGCTCGATCGTGATCAAGCTGC
CGATGCCCCCTGGTTGTCTGCCGACGCTGTGGCAGCAGGAGGATCGCTTCAAGGAATCCTACCTCAGCGAATTCCCCGGC
TACTACAAGACCTCCGATGCCGGCTTCAAGGATGAGGACGGCTATGTCTTCGTCATGGGCCGCACCGACGACATCATCAA
CGTTGCCGGCCATCGGCTGTCGACCGGCGGCATGGAGGAAATCCTGGCGTCGCATCCCGATGTCGCCGAATGCGCCGTGC
TCGGCATCAAGGACGCGATCAAGGGCGAGGTGCCCTGCGGCTTCCTGGTGCTCAAGGCCGGGGTCTCGCGCAGCCCGTCG
GTGATCGAGAAGGAGATCGTCGCGCTGGTGCGCGACAAGCTCGGCCCGGTCGCGGCGTTCAAGCTCGCCATCACCGTCGG
CCGCCTGCCGAAGACACGCTCCGGCAAGATCCTGCGCGGCACCATCAAGAAGATCGCCGACGGCGAGCAGTGGAGCATGC
CGGCCACGATCGAGGACCCCAAGGTTCTCGAGGAGATCGGCGAGGCGCTGAAAAGCCGGGTGTAG

Upstream 100 bases:

>100_bases
CCGCACGCCCGTGCCTGATCTGGATCAATTCATGATGGCTTGAACTGTCGCTTTGTCCCGGACAATAGTTGGGAATAAAC
ACAGATCTTGGGGAAACGTC

Downstream 100 bases:

>100_bases
AGCGAGCGCTAGCGTTCGCCCGTTTCCACGTCGTCATGGCCGGGCTTGTCCCGGCCATCCACGTCGAGCGGCCCCTTGGG
AACGACGTGGATGCCCGTGA

Product: putative propionate--CoA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MSAASRYHEVHARSLQDPEGFWAEAARAIDWIEPAKTIFDPSMGAYGRWFAGAVVNTCFNALDRHVAAGRGEQLALIHDS
PLTNSVTRITYAEMLKEVQTLAAIMQDFGVGKGDRVILYMPMVPEAMVAMLACARIGAVHSVVFGGFAAKELATRIDDAT
PKLVLSASCGIEPGRIVQYKPLLDQAIELADKRPQACIILQRPEHVCELKPGRDYDWAALRKSAIANGKLAPCVPVLATD
PLYILYTSGTTGKPKGVVRDNGGHLVALKWSMENLYGIKPGEVWWCGSDIGWVVGHSYIIYGPLIHGATSVMYEGKPVGT
PDAGAFWRVIADHKAVALFTAPTAFRAIRKEDPEGTLLSKYDLSQFRTLFLAGERADPPTVEWAEQQLKVPVIDHWWQTE
TGWCIAGNPVGLGLLPVKHGSPTVPMPGYQVDIVDEAAKPVPANTMGSIVIKLPMPPGCLPTLWQQEDRFKESYLSEFPG
YYKTSDAGFKDEDGYVFVMGRTDDIINVAGHRLSTGGMEEILASHPDVAECAVLGIKDAIKGEVPCGFLVLKAGVSRSPS
VIEKEIVALVRDKLGPVAAFKLAITVGRLPKTRSGKILRGTIKKIADGEQWSMPATIEDPKVLEEIGEALKSRV

Sequences:

>Translated_634_residues
MSAASRYHEVHARSLQDPEGFWAEAARAIDWIEPAKTIFDPSMGAYGRWFAGAVVNTCFNALDRHVAAGRGEQLALIHDS
PLTNSVTRITYAEMLKEVQTLAAIMQDFGVGKGDRVILYMPMVPEAMVAMLACARIGAVHSVVFGGFAAKELATRIDDAT
PKLVLSASCGIEPGRIVQYKPLLDQAIELADKRPQACIILQRPEHVCELKPGRDYDWAALRKSAIANGKLAPCVPVLATD
PLYILYTSGTTGKPKGVVRDNGGHLVALKWSMENLYGIKPGEVWWCGSDIGWVVGHSYIIYGPLIHGATSVMYEGKPVGT
PDAGAFWRVIADHKAVALFTAPTAFRAIRKEDPEGTLLSKYDLSQFRTLFLAGERADPPTVEWAEQQLKVPVIDHWWQTE
TGWCIAGNPVGLGLLPVKHGSPTVPMPGYQVDIVDEAAKPVPANTMGSIVIKLPMPPGCLPTLWQQEDRFKESYLSEFPG
YYKTSDAGFKDEDGYVFVMGRTDDIINVAGHRLSTGGMEEILASHPDVAECAVLGIKDAIKGEVPCGFLVLKAGVSRSPS
VIEKEIVALVRDKLGPVAAFKLAITVGRLPKTRSGKILRGTIKKIADGEQWSMPATIEDPKVLEEIGEALKSRV
>Mature_633_residues
SAASRYHEVHARSLQDPEGFWAEAARAIDWIEPAKTIFDPSMGAYGRWFAGAVVNTCFNALDRHVAAGRGEQLALIHDSP
LTNSVTRITYAEMLKEVQTLAAIMQDFGVGKGDRVILYMPMVPEAMVAMLACARIGAVHSVVFGGFAAKELATRIDDATP
KLVLSASCGIEPGRIVQYKPLLDQAIELADKRPQACIILQRPEHVCELKPGRDYDWAALRKSAIANGKLAPCVPVLATDP
LYILYTSGTTGKPKGVVRDNGGHLVALKWSMENLYGIKPGEVWWCGSDIGWVVGHSYIIYGPLIHGATSVMYEGKPVGTP
DAGAFWRVIADHKAVALFTAPTAFRAIRKEDPEGTLLSKYDLSQFRTLFLAGERADPPTVEWAEQQLKVPVIDHWWQTET
GWCIAGNPVGLGLLPVKHGSPTVPMPGYQVDIVDEAAKPVPANTMGSIVIKLPMPPGCLPTLWQQEDRFKESYLSEFPGY
YKTSDAGFKDEDGYVFVMGRTDDIINVAGHRLSTGGMEEILASHPDVAECAVLGIKDAIKGEVPCGFLVLKAGVSRSPSV
IEKEIVALVRDKLGPVAAFKLAITVGRLPKTRSGKILRGTIKKIADGEQWSMPATIEDPKVLEEIGEALKSRV

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=637, Percent_Identity=50.5494505494505, Blast_Score=682, Evalue=0.0,
Organism=Homo sapiens, GI8923896, Length=660, Percent_Identity=35.4545454545455, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI253970489, Length=670, Percent_Identity=35.3731343283582, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI28416953, Length=632, Percent_Identity=37.1835443037975, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI38505220, Length=525, Percent_Identity=27.4285714285714, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI115511026, Length=523, Percent_Identity=26.9598470363289, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI42544132, Length=520, Percent_Identity=26.9230769230769, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI31982927, Length=639, Percent_Identity=25.0391236306729, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI58082049, Length=530, Percent_Identity=26.0377358490566, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI157311624, Length=534, Percent_Identity=26.0299625468165, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI157311622, Length=534, Percent_Identity=26.0299625468165, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI122937307, Length=499, Percent_Identity=26.25250501002, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI156151445, Length=594, Percent_Identity=22.8956228956229, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI195947378, Length=432, Percent_Identity=23.6111111111111, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42544134, Length=369, Percent_Identity=22.4932249322493, Blast_Score=85, Evalue=3e-16,
Organism=Homo sapiens, GI187761345, Length=394, Percent_Identity=25.3807106598985, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI187761343, Length=394, Percent_Identity=25.3807106598985, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1786529, Length=631, Percent_Identity=45.324881141046, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1790505, Length=634, Percent_Identity=38.1703470031546, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI221142682, Length=557, Percent_Identity=24.5960502692998, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788107, Length=554, Percent_Identity=24.187725631769, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI145693145, Length=525, Percent_Identity=25.1428571428571, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786801, Length=525, Percent_Identity=23.6190476190476, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=649, Percent_Identity=32.9738058551618, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI71982997, Length=649, Percent_Identity=32.9738058551618, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17569507, Length=646, Percent_Identity=22.7554179566563, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71985884, Length=593, Percent_Identity=23.4401349072513, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=564, Percent_Identity=22.1631205673759, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=544, Percent_Identity=24.0808823529412, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=561, Percent_Identity=24.0641711229947, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=22.962962962963, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538037, Length=181, Percent_Identity=29.2817679558011, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=646, Percent_Identity=34.8297213622291, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323182, Length=648, Percent_Identity=35.0308641975309, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319699, Length=546, Percent_Identity=23.4432234432234, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21355225, Length=632, Percent_Identity=46.5189873417722, Blast_Score=618, Evalue=1e-177,
Organism=Drosophila melanogaster, GI24667955, Length=654, Percent_Identity=36.697247706422, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI62472339, Length=558, Percent_Identity=37.6344086021505, Blast_Score=361, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24667959, Length=527, Percent_Identity=37.1916508538899, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI21355181, Length=545, Percent_Identity=22.2018348623853, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI18859661, Length=563, Percent_Identity=23.6234458259325, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=375, Percent_Identity=25.6, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=368, Percent_Identity=22.8260869565217, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356441, Length=373, Percent_Identity=22.5201072386059, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24653035, Length=376, Percent_Identity=24.468085106383, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356947, Length=371, Percent_Identity=22.911051212938, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 68931; Mature: 68800

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAASRYHEVHARSLQDPEGFWAEAARAIDWIEPAKTIFDPSMGAYGRWFAGAVVNTCFN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ALDRHVAAGRGEQLALIHDSPLTNSVTRITYAEMLKEVQTLAAIMQDFGVGKGDRVILYM
HHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PMVPEAMVAMLACARIGAVHSVVFGGFAAKELATRIDDATPKLVLSASCGIEPGRIVQYK
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEH
PLLDQAIELADKRPQACIILQRPEHVCELKPGRDYDWAALRKSAIANGKLAPCVPVLATD
HHHHHHHHHHCCCCCEEEEEECCCHHEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEECC
PLYILYTSGTTGKPKGVVRDNGGHLVALKWSMENLYGIKPGEVWWCGSDIGWVVGHSYII
CEEEEEECCCCCCCCCEEECCCCEEEEEEEEHHHHCCCCCCCEEEECCCCCHHHCCCEEE
YGPLIHGATSVMYEGKPVGTPDAGAFWRVIADHKAVALFTAPTAFRAIRKEDPEGTLLSK
EECHHHCCHHEEECCCCCCCCCCCHHHHEECCCCEEEEEECCHHHHHHHCCCCCCCEEEH
YDLSQFRTLFLAGERADPPTVEWAEQQLKVPVIDHWWQTETGWCIAGNPVGLGLLPVKHG
HHHHHHHEEEEECCCCCCCCHHHHHHHCCCCEECCCCCCCCCEEEECCCCCEEEEEECCC
SPTVPMPGYQVDIVDEAAKPVPANTMGSIVIKLPMPPGCLPTLWQQEDRFKESYLSEFPG
CCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
YYKTSDAGFKDEDGYVFVMGRTDDIINVAGHRLSTGGMEEILASHPDVAECAVLGIKDAI
CEECCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHCCHHHH
KGEVPCGFLVLKAGVSRSPSVIEKEIVALVRDKLGPVAAFKLAITVGRLPKTRSGKILRG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCHHH
TIKKIADGEQWSMPATIEDPKVLEEIGEALKSRV
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAASRYHEVHARSLQDPEGFWAEAARAIDWIEPAKTIFDPSMGAYGRWFAGAVVNTCFN
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ALDRHVAAGRGEQLALIHDSPLTNSVTRITYAEMLKEVQTLAAIMQDFGVGKGDRVILYM
HHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PMVPEAMVAMLACARIGAVHSVVFGGFAAKELATRIDDATPKLVLSASCGIEPGRIVQYK
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEH
PLLDQAIELADKRPQACIILQRPEHVCELKPGRDYDWAALRKSAIANGKLAPCVPVLATD
HHHHHHHHHHCCCCCEEEEEECCCHHEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEECC
PLYILYTSGTTGKPKGVVRDNGGHLVALKWSMENLYGIKPGEVWWCGSDIGWVVGHSYII
CEEEEEECCCCCCCCCEEECCCCEEEEEEEEHHHHCCCCCCCEEEECCCCCHHHCCCEEE
YGPLIHGATSVMYEGKPVGTPDAGAFWRVIADHKAVALFTAPTAFRAIRKEDPEGTLLSK
EECHHHCCHHEEECCCCCCCCCCCHHHHEECCCCEEEEEECCHHHHHHHCCCCCCCEEEH
YDLSQFRTLFLAGERADPPTVEWAEQQLKVPVIDHWWQTETGWCIAGNPVGLGLLPVKHG
HHHHHHHEEEEECCCCCCCCHHHHHHHCCCCEECCCCCCCCCEEEECCCCCEEEEEECCC
SPTVPMPGYQVDIVDEAAKPVPANTMGSIVIKLPMPPGCLPTLWQQEDRFKESYLSEFPG
CCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
YYKTSDAGFKDEDGYVFVMGRTDDIINVAGHRLSTGGMEEILASHPDVAECAVLGIKDAI
CEECCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHCCHHHH
KGEVPCGFLVLKAGVSRSPSVIEKEIVALVRDKLGPVAAFKLAITVGRLPKTRSGKILRG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCHHH
TIKKIADGEQWSMPATIEDPKVLEEIGEALKSRV
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]