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The map label for this gene is rnhA [H]

Identifier: 146343359

GI number: 146343359

Start: 6836651

End: 6837115

Strand: Reverse

Name: rnhA [H]

Synonym: BRADO6586

Alternate gene names: 146343359

Gene position: 6837115-6836651 (Counterclockwise)

Preceding gene: 146343360

Following gene: 146343353

Centisome position: 91.69

GC content: 64.95

Gene sequence:

>465_bases
ATGAGCGAGCTTCCGGTCGTCAGCATCTTCACCGACGGCGCCTGCTCCGGAAATCCCGGCCCTGGCGGCTGGGGCGCGAT
CCTGCGCTTCGGCGACAAGGAGAAGGAGCTCAAGGGCGGCGAGCCGCATACCACGAACAACCGCATGGAGCTGATGGCGG
CGATCTCGGCGCTGGAGGCGCTGAAGAAGTCGTGCCAGGTCGAGCTTTACACCGACAGCCAATATGTCCGGCAGGGCATC
ACCGGCTGGATCCACGGCTGGAAGCGCAACGGCTGGAAGACCGCCGACAAGAAGCCGGTCAAGAACGCCGAACTCTGGCA
ACGCCTCGATGCCGCGCTGAAACCGCACAAGATCAACTGGCACTGGGTCAAGGGCCACGCCGGCCATCCCGAGAACGAGC
GCGCCGACCAATTGGCGCGTGATGGCGTGGCGATGGCGCGGATGCAGAAGAACGTGCGGAGCTGA

Upstream 100 bases:

>100_bases
GCGCGCTGGTGAAGCCGAAGGATCCGCTCGAATATGTCCGCAAGCTGCGCTTCCATCAGGCGGTGACCAGCGCGCGCGAC
TACGGCATCGGAGCATCCGC

Downstream 100 bases:

>100_bases
TGAGCTAGTAAGGCGGTGTCGCCTCAACCAAAGCCGTCATCGCCCGGCTTGACCGGGCGATCCAGTATTCACCAGCAGTC
GTGTTCAACCGAGAGGCCGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSELPVVSIFTDGACSGNPGPGGWGAILRFGDKEKELKGGEPHTTNNRMELMAAISALEALKKSCQVELYTDSQYVRQGI
TGWIHGWKRNGWKTADKKPVKNAELWQRLDAALKPHKINWHWVKGHAGHPENERADQLARDGVAMARMQKNVRS

Sequences:

>Translated_154_residues
MSELPVVSIFTDGACSGNPGPGGWGAILRFGDKEKELKGGEPHTTNNRMELMAAISALEALKKSCQVELYTDSQYVRQGI
TGWIHGWKRNGWKTADKKPVKNAELWQRLDAALKPHKINWHWVKGHAGHPENERADQLARDGVAMARMQKNVRS
>Mature_153_residues
SELPVVSIFTDGACSGNPGPGGWGAILRFGDKEKELKGGEPHTTNNRMELMAAISALEALKKSCQVELYTDSQYVRQGIT
GWIHGWKRNGWKTADKKPVKNAELWQRLDAALKPHKINWHWVKGHAGHPENERADQLARDGVAMARMQKNVRS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=148, Percent_Identity=40.5405405405405, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1786408, Length=137, Percent_Identity=73.7226277372263, Blast_Score=214, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI115532542, Length=152, Percent_Identity=40.1315789473684, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17505482, Length=131, Percent_Identity=32.0610687022901, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552509, Length=155, Percent_Identity=36.7741935483871, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137476, Length=155, Percent_Identity=36.7741935483871, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17198; Mature: 17067

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELPVVSIFTDGACSGNPGPGGWGAILRFGDKEKELKGGEPHTTNNRMELMAAISALEA
CCCCCEEEEEECCCCCCCCCCCCHHHHEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
LKKSCQVELYTDSQYVRQGITGWIHGWKRNGWKTADKKPVKNAELWQRLDAALKPHKINW
HHHHCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
HWVKGHAGHPENERADQLARDGVAMARMQKNVRS
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELPVVSIFTDGACSGNPGPGGWGAILRFGDKEKELKGGEPHTTNNRMELMAAISALEA
CCCCEEEEEECCCCCCCCCCCCHHHHEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
LKKSCQVELYTDSQYVRQGITGWIHGWKRNGWKTADKKPVKNAELWQRLDAALKPHKINW
HHHHCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
HWVKGHAGHPENERADQLARDGVAMARMQKNVRS
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA