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The map label for this gene is ppdK [H]

Identifier: 146343155

GI number: 146343155

Start: 6608719

End: 6610314

Strand: Reverse

Name: ppdK [H]

Synonym: BRADO6353

Alternate gene names: 146343155

Gene position: 6610314-6608719 (Counterclockwise)

Preceding gene: 146343156

Following gene: 146343150

Centisome position: 88.65

GC content: 69.17

Gene sequence:

>1596_bases
GTGAGGACCAAGAGCATGATCGTCAGAATTGGGGATGGCCGCCGCGAGCTGCCAAGCGCGGACGAGGTCGGCGCCAAGGC
GGCCAATCTTGCGCGCATGGCCGCGCTGGATCTGCCGGTGCCGCCCGCCTTCGTGCTGCCCGTGCAGCTCTGCGCCGATG
TCGTCGCCGGCAAGGGCCAGGCGACACGCCAGCTCGCCGAGGGACTGAAAGAGGGGATTGCCTATCTCGAGCAGGCCACC
GGAAAAACCTTCGGCTGCTCGCGCAACCCGCTGCTCGTGTCCGTGAGATCCGGCGCTGCGCGCTCGATGCCGGGCATGCT
CGACACTGTCCTCAATGTCGGCTGCACGTCGCGCGCGGTGCGTGGCCTGGTGCGCGCCACCGGCCGGCCGCGACTGGCGT
GGGACTGCCGCCGGCGGCTGCTCGAGAGCTACGCCGAGACGGTGATCGGCCTCGAGGCCGCTGCGCTGGCGGAGCGTTTG
AACCAGTTGATTGCCGCTGAAGGCGTGAGCAACGAGCGCGAGCTGGACAGCGAGGCGCTCGAGCGGCTGGCTGCCGAGGA
ACGCAGGCTGCTGGAGGATAACGACGATGACGGCTGGCTCGAGGATGCCGCGGTCCAGCTCGAGCGCGCAGCGTTCGCCG
TCTATCGCTCCTGGACCAGCGAGCGGGCGAAGACCTATCGGCGGCTCGAAGCTCTGGAGCATCTGACAGGCACAGCGGTC
ACGGTGCAGGCCATGGTGTTCGGCAATGGCGGCACGTCGTCCGGGGCGGGCGTCGCGTTCTCGCGCGATCCCTCGACGGG
CGTCGACGAGCCGGTGATCGACGTGCTGTTCGATTCGCAGGGCGAGGACGTCGTCTCGGGAAGACGGACGCCTGAGACCG
AGGCCGCGTTGTCCCGATCATTGCCGCGTGTCGCGCAGCAGCTGCGGGCAACGCTGAAGCGGCTCGAAGCCGAGTTTCGC
GATGTGCAGGACATCGAGTTCACGATCGAGGAAGGCCGTTTGTGGATTCTGCAGACACGCGCGGCCAAGCGCACGCCGCG
CGCGGCGCTGCGCATCGCGGTCGGTCTGGTGCATGAGGGGGTGATCTCCCAGGACGAGGCGCTTGCAAGACTGCAGGGAA
TCGACCTGGAGGCGCTGACGGAAACGTCGCTCGTCGCGGTCGGCGAGCCTGCGGCGGTGGGAGTTGCCGCATCCGGCGGC
GTCGCGGCCGGGCGCGCGGCGTTCGACAGCGCGTCGGCGGAGCGGCTCGCGGCAGCCGGAGATCCAGTGATTTTGATCCG
CCCTGACACCAGCACCGCCGATGTCGCAGGCTTTGCCGCGGCCGTTGGCATCGCCACGGCGGTGGGAGCTCGCACGGCGC
ATGCGTCGCTCGTTGCGCGGCAGATGGGCAAGCCCTGCGTGGTCGGCTGCGCCGCCATCCGCTTCGACGAGGACGGCCAT
CAGGCGTGCATCGGTGACACGATCATTGCTCCGGGTGACTGGATCACGATCGATGGCAATGCCGGCCGGGTCTATCTGGG
GCGCGGCGAGATCGTGGCGCGTCGGCCGGAAGCCGAGCTTGCGGAGCTTTCGACCTGGCGCTCCGCCGCCGCTTGA

Upstream 100 bases:

>100_bases
TGGGCGCGCTCGAGCTGGCGCGCGCGATCGAGCGCGCCACCGACTGCCTGGCGAGCTTTGGACACCTGCTGCGGCGCTAC
ACGATGAGTGATCTTTCGGC

Downstream 100 bases:

>100_bases
GACGCCGGCACGGTCGCCCAGCCGGGGCTGGACGACCGGAGGACCCGGGGTGTCAGTTGCGGGTTGGCGCCGGCACGGCC
GTACGGTTCGCCGGCACCGT

Product: putative pyruvate, phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MRTKSMIVRIGDGRRELPSADEVGAKAANLARMAALDLPVPPAFVLPVQLCADVVAGKGQATRQLAEGLKEGIAYLEQAT
GKTFGCSRNPLLVSVRSGAARSMPGMLDTVLNVGCTSRAVRGLVRATGRPRLAWDCRRRLLESYAETVIGLEAAALAERL
NQLIAAEGVSNERELDSEALERLAAEERRLLEDNDDDGWLEDAAVQLERAAFAVYRSWTSERAKTYRRLEALEHLTGTAV
TVQAMVFGNGGTSSGAGVAFSRDPSTGVDEPVIDVLFDSQGEDVVSGRRTPETEAALSRSLPRVAQQLRATLKRLEAEFR
DVQDIEFTIEEGRLWILQTRAAKRTPRAALRIAVGLVHEGVISQDEALARLQGIDLEALTETSLVAVGEPAAVGVAASGG
VAAGRAAFDSASAERLAAAGDPVILIRPDTSTADVAGFAAAVGIATAVGARTAHASLVARQMGKPCVVGCAAIRFDEDGH
QACIGDTIIAPGDWITIDGNAGRVYLGRGEIVARRPEAELAELSTWRSAAA

Sequences:

>Translated_531_residues
MRTKSMIVRIGDGRRELPSADEVGAKAANLARMAALDLPVPPAFVLPVQLCADVVAGKGQATRQLAEGLKEGIAYLEQAT
GKTFGCSRNPLLVSVRSGAARSMPGMLDTVLNVGCTSRAVRGLVRATGRPRLAWDCRRRLLESYAETVIGLEAAALAERL
NQLIAAEGVSNERELDSEALERLAAEERRLLEDNDDDGWLEDAAVQLERAAFAVYRSWTSERAKTYRRLEALEHLTGTAV
TVQAMVFGNGGTSSGAGVAFSRDPSTGVDEPVIDVLFDSQGEDVVSGRRTPETEAALSRSLPRVAQQLRATLKRLEAEFR
DVQDIEFTIEEGRLWILQTRAAKRTPRAALRIAVGLVHEGVISQDEALARLQGIDLEALTETSLVAVGEPAAVGVAASGG
VAAGRAAFDSASAERLAAAGDPVILIRPDTSTADVAGFAAAVGIATAVGARTAHASLVARQMGKPCVVGCAAIRFDEDGH
QACIGDTIIAPGDWITIDGNAGRVYLGRGEIVARRPEAELAELSTWRSAAA
>Mature_531_residues
MRTKSMIVRIGDGRRELPSADEVGAKAANLARMAALDLPVPPAFVLPVQLCADVVAGKGQATRQLAEGLKEGIAYLEQAT
GKTFGCSRNPLLVSVRSGAARSMPGMLDTVLNVGCTSRAVRGLVRATGRPRLAWDCRRRLLESYAETVIGLEAAALAERL
NQLIAAEGVSNERELDSEALERLAAEERRLLEDNDDDGWLEDAAVQLERAAFAVYRSWTSERAKTYRRLEALEHLTGTAV
TVQAMVFGNGGTSSGAGVAFSRDPSTGVDEPVIDVLFDSQGEDVVSGRRTPETEAALSRSLPRVAQQLRATLKRLEAEFR
DVQDIEFTIEEGRLWILQTRAAKRTPRAALRIAVGLVHEGVISQDEALARLQGIDLEALTETSLVAVGEPAAVGVAASGG
VAAGRAAFDSASAERLAAAGDPVILIRPDTSTADVAGFAAAVGIATAVGARTAHASLVARQMGKPCVVGCAAIRFDEDGH
QACIGDTIIAPGDWITIDGNAGRVYLGRGEIVARRPEAELAELSTWRSAAA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 56176; Mature: 56176

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00893 NUDIX ; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKSMIVRIGDGRRELPSADEVGAKAANLARMAALDLPVPPAFVLPVQLCADVVAGKGQ
CCCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
ATRQLAEGLKEGIAYLEQATGKTFGCSRNPLLVSVRSGAARSMPGMLDTVLNVGCTSRAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHH
RGLVRATGRPRLAWDCRRRLLESYAETVIGLEAAALAERLNQLIAAEGVSNERELDSEAL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHH
ERLAAEERRLLEDNDDDGWLEDAAVQLERAAFAVYRSWTSERAKTYRRLEALEHLTGTAV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
TVQAMVFGNGGTSSGAGVAFSRDPSTGVDEPVIDVLFDSQGEDVVSGRRTPETEAALSRS
EEEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHCCCCCCHHHHHHHHH
LPRVAQQLRATLKRLEAEFRDVQDIEFTIEEGRLWILQTRAAKRTPRAALRIAVGLVHEG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHC
VISQDEALARLQGIDLEALTETSLVAVGEPAAVGVAASGGVAAGRAAFDSASAERLAAAG
CCCHHHHHHHHHCCCHHHHCCCCEEEECCCCEEEEECCCCCCCCHHHHCCCCHHHHHCCC
DPVILIRPDTSTADVAGFAAAVGIATAVGARTAHASLVARQMGKPCVVGCAAIRFDEDGH
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
QACIGDTIIAPGDWITIDGNAGRVYLGRGEIVARRPEAELAELSTWRSAAA
CEECCCEEECCCCEEEEECCCCEEEEECCCEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTKSMIVRIGDGRRELPSADEVGAKAANLARMAALDLPVPPAFVLPVQLCADVVAGKGQ
CCCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
ATRQLAEGLKEGIAYLEQATGKTFGCSRNPLLVSVRSGAARSMPGMLDTVLNVGCTSRAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHH
RGLVRATGRPRLAWDCRRRLLESYAETVIGLEAAALAERLNQLIAAEGVSNERELDSEAL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHH
ERLAAEERRLLEDNDDDGWLEDAAVQLERAAFAVYRSWTSERAKTYRRLEALEHLTGTAV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
TVQAMVFGNGGTSSGAGVAFSRDPSTGVDEPVIDVLFDSQGEDVVSGRRTPETEAALSRS
EEEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHCCCCCCHHHHHHHHH
LPRVAQQLRATLKRLEAEFRDVQDIEFTIEEGRLWILQTRAAKRTPRAALRIAVGLVHEG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHC
VISQDEALARLQGIDLEALTETSLVAVGEPAAVGVAASGGVAAGRAAFDSASAERLAAAG
CCCHHHHHHHHHCCCHHHHCCCCEEEECCCCEEEEECCCCCCCCHHHHCCCCHHHHHCCC
DPVILIRPDTSTADVAGFAAAVGIATAVGARTAHASLVARQMGKPCVVGCAAIRFDEDGH
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
QACIGDTIIAPGDWITIDGNAGRVYLGRGEIVARRPEAELAELSTWRSAAA
CEECCCEEECCCCEEEEECCCCEEEEECCCEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]