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The map label for this gene is gspE [H]

Identifier: 146343150

GI number: 146343150

Start: 6600458

End: 6602110

Strand: Reverse

Name: gspE [H]

Synonym: BRADO6348

Alternate gene names: 146343150

Gene position: 6602110-6600458 (Counterclockwise)

Preceding gene: 146343155

Following gene: 146343149

Centisome position: 88.54

GC content: 68.24

Gene sequence:

>1653_bases
ATGAGCGGTATGCTGTCGACCCAGTTTCACGACTATCTCTCCCAGCGCCACCCGCAGGCGGCTGACAGCGGAGCTGCGGG
CGAGGCACAGGTCGCGCTCTGGGACAAGTTCGGCCTGAGCGCCGAGGCGTTCGCCGAGGAGGCGGCTCGCTTCACCGGGT
TGGAGCGCGTCTCGTTGCGCGAGCTGCTCGCGGCCGTTCCGGCAACGGCAGAGTTCTCCGATCGCTTCCTGCGCGAGGTG
GCCGGTTATCCCTATCAGACGCCCGAACGCGTCGCCGTGTTCGCAGTCGCCGATCCGACCGATCAGGCGGTCCGCAGAGC
GGCCGAGCTCGTGCTCGGTCCGGCGACGGTCTTCAAGGTCGCCTCGTACGAGGATGTCGCCGTCGTCCTCGACCAGCGCC
TCGGCGGCACCGACGATTCGGAAATCTCCCCTCAGCAGGAGCTGCGCGACGACGACGTCGAAAGCCTGCGCGATCTCGCC
AGCGGCGCGCCGGTCGTCCGCGCCGTCAACGATCTGATCGAGAAGGCGGTCGAGATGCGCGCCAGCGACATCCACATCGA
GCCGTTCTCGACCGGTCTCGTGGTCAGACTGCGCGTCGACGGCCTGCTGCGCCCGATCGCGGCGCCGGCGGGCGTGCTGC
CGCAGGCCGTAATCTCGCGCATCAAGATCGTTGCCGGATTGAACATTGCCGAGCGTCGGCTGCCACAGGATGGTGCCGCG
CGCATTCGCGCCGGCCGTACCGAGATCGATGTCCGCGTCGCCATCATGCCGACGCAGGCCGGCGAGTCGGCGGTGCTGCG
CATCTTGCCCAAGGATCGGTCGCAACTGGTCGCGAATCGTCTGGGGCTGTCCGCGCGGGACGACGCCGCGCTGCGGCGCC
TGATCACGCTGCCGCACGGCATGATCGTGATCACCGGACCGACTGGCAGCGGCAAGACCACGACGCTGGCGACGATCCTG
TCGATGCTCAACACCCCCGAGCGCAAGATTCTCACCGTCGAGGATCCCGTGGAATACGAGATCCCCGGCATCAACCAGTC
GCAGATCAAGCCGGCGATCGGACTGACCTTCGCCAGCGCGCTGCGCTCCTTCGTGCGTCAGGACCCCGATGTCATCATGG
TCGGCGAGATCCGAGACAGCGAGACGGCGCATGTCGCCATCCATGCGGCGCTGACCGGCCATCTCGTGCTGACCACGCTG
CACACCGAGACGGCGGCAGCCGCGGTGCCGCGGCTGCTCGATCTCGGCGTCGAGGGCTATCTGTTGCGATCGACCCTGCG
CGCGGTCATTGCCCAGCGCCTCGTGCGTCAGCTGTGCGAGCACTGCAAGACGGCGCGTCCGCTGGATGAGGCGACGTGCC
GGGATGATCCCCGCTACAGCCATGTCGGCCTCGTGCCCGGCGACATCGTCTGGTATCCGGCGGGATGCGAGCGCTGCGGC
CACAGCGGCTACCGTGGCCGGGTCGGCGTGTTCGAGCTGCTTGAGCTGACCTCCGGCCTGCGCGAGCTGATCGCCGACAG
CGCCGATGGTCTCGCGATCGACCGGCGGGCGATCCAGGAGGGCATGACGACGATGCTCGACGACGGGGTCGCCAAATGCC
GCGCCGGCGTCACCTCGGCGTCCGAACTGTTCCGTGTCCTCGTCGTGCGTTGA

Upstream 100 bases:

>100_bases
CCCGATTTCGTACAGCCGTCATGTTCGCGATCGATAACGGCGGCAGCCAGGAGCGGGGTGCCGAGACCAGGGGCCGGCAC
GGGCGCCACCGGATCACGCC

Downstream 100 bases:

>100_bases
GGTGGATCGTGCCGAGCTACCGCTATCGCGCTTTGACACAGTCCGGCGAGATCGTCGTCGGATCGCTCGATGCGCCGTCG
CGCGGCGAGGTCGAGCGCCG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSGMLSTQFHDYLSQRHPQAADSGAAGEAQVALWDKFGLSAEAFAEEAARFTGLERVSLRELLAAVPATAEFSDRFLREV
AGYPYQTPERVAVFAVADPTDQAVRRAAELVLGPATVFKVASYEDVAVVLDQRLGGTDDSEISPQQELRDDDVESLRDLA
SGAPVVRAVNDLIEKAVEMRASDIHIEPFSTGLVVRLRVDGLLRPIAAPAGVLPQAVISRIKIVAGLNIAERRLPQDGAA
RIRAGRTEIDVRVAIMPTQAGESAVLRILPKDRSQLVANRLGLSARDDAALRRLITLPHGMIVITGPTGSGKTTTLATIL
SMLNTPERKILTVEDPVEYEIPGINQSQIKPAIGLTFASALRSFVRQDPDVIMVGEIRDSETAHVAIHAALTGHLVLTTL
HTETAAAAVPRLLDLGVEGYLLRSTLRAVIAQRLVRQLCEHCKTARPLDEATCRDDPRYSHVGLVPGDIVWYPAGCERCG
HSGYRGRVGVFELLELTSGLRELIADSADGLAIDRRAIQEGMTTMLDDGVAKCRAGVTSASELFRVLVVR

Sequences:

>Translated_550_residues
MSGMLSTQFHDYLSQRHPQAADSGAAGEAQVALWDKFGLSAEAFAEEAARFTGLERVSLRELLAAVPATAEFSDRFLREV
AGYPYQTPERVAVFAVADPTDQAVRRAAELVLGPATVFKVASYEDVAVVLDQRLGGTDDSEISPQQELRDDDVESLRDLA
SGAPVVRAVNDLIEKAVEMRASDIHIEPFSTGLVVRLRVDGLLRPIAAPAGVLPQAVISRIKIVAGLNIAERRLPQDGAA
RIRAGRTEIDVRVAIMPTQAGESAVLRILPKDRSQLVANRLGLSARDDAALRRLITLPHGMIVITGPTGSGKTTTLATIL
SMLNTPERKILTVEDPVEYEIPGINQSQIKPAIGLTFASALRSFVRQDPDVIMVGEIRDSETAHVAIHAALTGHLVLTTL
HTETAAAAVPRLLDLGVEGYLLRSTLRAVIAQRLVRQLCEHCKTARPLDEATCRDDPRYSHVGLVPGDIVWYPAGCERCG
HSGYRGRVGVFELLELTSGLRELIADSADGLAIDRRAIQEGMTTMLDDGVAKCRAGVTSASELFRVLVVR
>Mature_549_residues
SGMLSTQFHDYLSQRHPQAADSGAAGEAQVALWDKFGLSAEAFAEEAARFTGLERVSLRELLAAVPATAEFSDRFLREVA
GYPYQTPERVAVFAVADPTDQAVRRAAELVLGPATVFKVASYEDVAVVLDQRLGGTDDSEISPQQELRDDDVESLRDLAS
GAPVVRAVNDLIEKAVEMRASDIHIEPFSTGLVVRLRVDGLLRPIAAPAGVLPQAVISRIKIVAGLNIAERRLPQDGAAR
IRAGRTEIDVRVAIMPTQAGESAVLRILPKDRSQLVANRLGLSARDDAALRRLITLPHGMIVITGPTGSGKTTTLATILS
MLNTPERKILTVEDPVEYEIPGINQSQIKPAIGLTFASALRSFVRQDPDVIMVGEIRDSETAHVAIHAALTGHLVLTTLH
TETAAAAVPRLLDLGVEGYLLRSTLRAVIAQRLVRQLCEHCKTARPLDEATCRDDPRYSHVGLVPGDIVWYPAGCERCGH
SGYRGRVGVFELLELTSGLRELIADSADGLAIDRRAIQEGMTTMLDDGVAKCRAGVTSASELFRVLVVR

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=393, Percent_Identity=48.0916030534351, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1786296, Length=379, Percent_Identity=43.5356200527704, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=44.6153846153846, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 59394; Mature: 59263

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGMLSTQFHDYLSQRHPQAADSGAAGEAQVALWDKFGLSAEAFAEEAARFTGLERVSLR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCHHHHHH
ELLAAVPATAEFSDRFLREVAGYPYQTPERVAVFAVADPTDQAVRRAAELVLGPATVFKV
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHHEE
ASYEDVAVVLDQRLGGTDDSEISPQQELRDDDVESLRDLASGAPVVRAVNDLIEKAVEMR
CCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ASDIHIEPFSTGLVVRLRVDGLLRPIAAPAGVLPQAVISRIKIVAGLNIAERRLPQDGAA
HCCEEEEECCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHH
RIRAGRTEIDVRVAIMPTQAGESAVLRILPKDRSQLVANRLGLSARDDAALRRLITLPHG
HHHCCCEEEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCC
MIVITGPTGSGKTTTLATILSMLNTPERKILTVEDPVEYEIPGINQSQIKPAIGLTFASA
EEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHCCHHHHHHHHHH
LRSFVRQDPDVIMVGEIRDSETAHVAIHAALTGHLVLTTLHTETAAAAVPRLLDLGVEGY
HHHHHHCCCCEEEEECCCCCCCEEEEEEEEHHCEEEEEEECHHHHHHHHHHHHHCCCCHH
LLRSTLRAVIAQRLVRQLCEHCKTARPLDEATCRDDPRYSHVGLVPGDIVWYPAGCERCG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEECCCCCCEEEECCCHHHCC
HSGYRGRVGVFELLELTSGLRELIADSADGLAIDRRAIQEGMTTMLDDGVAKCRAGVTSA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SELFRVLVVR
HHHHHHHHCC
>Mature Secondary Structure 
SGMLSTQFHDYLSQRHPQAADSGAAGEAQVALWDKFGLSAEAFAEEAARFTGLERVSLR
CCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCHHHHHH
ELLAAVPATAEFSDRFLREVAGYPYQTPERVAVFAVADPTDQAVRRAAELVLGPATVFKV
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHHEE
ASYEDVAVVLDQRLGGTDDSEISPQQELRDDDVESLRDLASGAPVVRAVNDLIEKAVEMR
CCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ASDIHIEPFSTGLVVRLRVDGLLRPIAAPAGVLPQAVISRIKIVAGLNIAERRLPQDGAA
HCCEEEEECCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHH
RIRAGRTEIDVRVAIMPTQAGESAVLRILPKDRSQLVANRLGLSARDDAALRRLITLPHG
HHHCCCEEEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCC
MIVITGPTGSGKTTTLATILSMLNTPERKILTVEDPVEYEIPGINQSQIKPAIGLTFASA
EEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHCCHHHHHHHHHH
LRSFVRQDPDVIMVGEIRDSETAHVAIHAALTGHLVLTTLHTETAAAAVPRLLDLGVEGY
HHHHHHCCCCEEEEECCCCCCCEEEEEEEEHHCEEEEEEECHHHHHHHHHHHHHCCCCHH
LLRSTLRAVIAQRLVRQLCEHCKTARPLDEATCRDDPRYSHVGLVPGDIVWYPAGCERCG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEECCCCCCEEEECCCHHHCC
HSGYRGRVGVFELLELTSGLRELIADSADGLAIDRRAIQEGMTTMLDDGVAKCRAGVTSA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SELFRVLVVR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1944223; 12024217 [H]