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The map label for this gene is yafC [C]

Identifier: 146342957

GI number: 146342957

Start: 6364704

End: 6365612

Strand: Reverse

Name: yafC [C]

Synonym: BRADO6140

Alternate gene names: 146342957

Gene position: 6365612-6364704 (Counterclockwise)

Preceding gene: 146342960

Following gene: 146342954

Centisome position: 85.37

GC content: 68.87

Gene sequence:

>909_bases
ATGGCGCGACTTCCGGATTTCGAGGCGATGGCGATCTTCGCCAAGGTCGTCGAGCTGCGCTCGTTTGCCGGAGCTGCGCA
GGAGCTGAGCCTCTCCAAAGCCTCGGTCTCGAAGGCGGTCAGCCGGCTCGAGGAGCGCCTGGGCACGCGGCTGTTCAACC
GGACGTCGCGGCGGCTGGCGCTGACCGATGCCGGCCAGCGCCTCTCCGAGCGTGCGGCACAGCTGCTCGCCGATGGCGAG
GCCGCGGAGACCGAGGCGCTGGCGCAGTCATTGACGCCGCGGGGCCTGGTGCGCTTCGCGGTGCCGATGACCTTCGGCGT
CAAGAAGATCGCTCCGCTGCTGCCGGCCTTCCTGGAGCAGTACCCCGAGGTCTCGATCGACCTGCATATGAGTGATGCCA
CCGTCGACCTGATCGGCGAGGGTTTCGACCTCGCTTTGCGTATCGCGCGCCTGCCGGATTCCTCGCTCGTCGCGCGCCGG
CTCTGTCCGATGCCGCGCTACACGGTGGCTGCGCCATCCTACCTGAAGCGCCACGGGCGGCCGACGCATCCGATGCATCT
CGCCGAGCACAAATGCTTCGGCTACGCCTATCTCTCGACGGCCGGCGTCTGGCACTACACCAATGCCGCGGGCGAGCCGG
CGAGCGTGCGCCCCGCCGGACAGCTGCGCGTCAACAATGGCGAGGCGCTGTTGCCGGCGGTGCTGGCGGGGCTCGGGATC
GCCGATCTGCCGGACTTCATCGTCGGTGATGCCATCGCGTCGGGCGAGGTCGAGGTTGTGCTGGAGGGCTGGAGCCAGCC
GGAGGGCGCGATGCATCTGGTGATGCCGCCCGGCGGCCCGCGGCCGGCGCGGGTCGAGGTGCTCTCGGAGTTCCTGGTCA
AAGAGCTCGCAAAAGCAAAGAAGCGGTGA

Upstream 100 bases:

>100_bases
GACTGGCCGCGTCGCGTTGGGCATTGGAATAAGGGAGCGGTCGGAGTATATAAATAGAAACTGTTGAAACCTATTGTTTC
CAAAAACGAGATTTGAGTCG

Downstream 100 bases:

>100_bases
TCGCGTGCGCCGCTCAGGCCTGCACCTCGCAGACGTCGACCCATTCGGCCGATGTCAGCTCGGCCATCCGCTCCGGCGTG
ATCTTCACCGCGCTGTGGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MARLPDFEAMAIFAKVVELRSFAGAAQELSLSKASVSKAVSRLEERLGTRLFNRTSRRLALTDAGQRLSERAAQLLADGE
AAETEALAQSLTPRGLVRFAVPMTFGVKKIAPLLPAFLEQYPEVSIDLHMSDATVDLIGEGFDLALRIARLPDSSLVARR
LCPMPRYTVAAPSYLKRHGRPTHPMHLAEHKCFGYAYLSTAGVWHYTNAAGEPASVRPAGQLRVNNGEALLPAVLAGLGI
ADLPDFIVGDAIASGEVEVVLEGWSQPEGAMHLVMPPGGPRPARVEVLSEFLVKELAKAKKR

Sequences:

>Translated_302_residues
MARLPDFEAMAIFAKVVELRSFAGAAQELSLSKASVSKAVSRLEERLGTRLFNRTSRRLALTDAGQRLSERAAQLLADGE
AAETEALAQSLTPRGLVRFAVPMTFGVKKIAPLLPAFLEQYPEVSIDLHMSDATVDLIGEGFDLALRIARLPDSSLVARR
LCPMPRYTVAAPSYLKRHGRPTHPMHLAEHKCFGYAYLSTAGVWHYTNAAGEPASVRPAGQLRVNNGEALLPAVLAGLGI
ADLPDFIVGDAIASGEVEVVLEGWSQPEGAMHLVMPPGGPRPARVEVLSEFLVKELAKAKKR
>Mature_301_residues
ARLPDFEAMAIFAKVVELRSFAGAAQELSLSKASVSKAVSRLEERLGTRLFNRTSRRLALTDAGQRLSERAAQLLADGEA
AETEALAQSLTPRGLVRFAVPMTFGVKKIAPLLPAFLEQYPEVSIDLHMSDATVDLIGEGFDLALRIARLPDSSLVARRL
CPMPRYTVAAPSYLKRHGRPTHPMHLAEHKCFGYAYLSTAGVWHYTNAAGEPASVRPAGQLRVNNGEALLPAVLAGLGIA
DLPDFIVGDAIASGEVEVVLEGWSQPEGAMHLVMPPGGPRPARVEVLSEFLVKELAKAKKR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=250, Percent_Identity=38, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=31.9727891156463, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=32.5259515570934, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787128, Length=274, Percent_Identity=31.7518248175182, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=32.1070234113712, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=30.5263157894737, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787589, Length=268, Percent_Identity=31.3432835820896, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788706, Length=252, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=25.6704980842912, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=128, Percent_Identity=37.5, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788748, Length=125, Percent_Identity=32.8, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=239, Percent_Identity=31.7991631799163, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=146, Percent_Identity=31.5068493150685, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32610; Mature: 32479

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLPDFEAMAIFAKVVELRSFAGAAQELSLSKASVSKAVSRLEERLGTRLFNRTSRRLA
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LTDAGQRLSERAAQLLADGEAAETEALAQSLTPRGLVRFAVPMTFGVKKIAPLLPAFLEQ
EHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YPEVSIDLHMSDATVDLIGEGFDLALRIARLPDSSLVARRLCPMPRYTVAAPSYLKRHGR
CCCCEEEEEECCCCHHHHCCCHHHEEHEECCCCHHHHHHHHCCCCCCCCCCCHHHHHCCC
PTHPMHLAEHKCFGYAYLSTAGVWHYTNAAGEPASVRPAGQLRVNNGEALLPAVLAGLGI
CCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCH
ADLPDFIVGDAIASGEVEVVLEGWSQPEGAMHLVMPPGGPRPARVEVLSEFLVKELAKAK
HCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
KR
CC
>Mature Secondary Structure 
ARLPDFEAMAIFAKVVELRSFAGAAQELSLSKASVSKAVSRLEERLGTRLFNRTSRRLA
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LTDAGQRLSERAAQLLADGEAAETEALAQSLTPRGLVRFAVPMTFGVKKIAPLLPAFLEQ
EHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YPEVSIDLHMSDATVDLIGEGFDLALRIARLPDSSLVARRLCPMPRYTVAAPSYLKRHGR
CCCCEEEEEECCCCHHHHCCCHHHEEHEECCCCHHHHHHHHCCCCCCCCCCCHHHHHCCC
PTHPMHLAEHKCFGYAYLSTAGVWHYTNAAGEPASVRPAGQLRVNNGEALLPAVLAGLGI
CCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCH
ADLPDFIVGDAIASGEVEVVLEGWSQPEGAMHLVMPPGGPRPARVEVLSEFLVKELAKAK
HCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]