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The map label for this gene is gcvA [H]

Identifier: 146342815

GI number: 146342815

Start: 6220768

End: 6221691

Strand: Direct

Name: gcvA [H]

Synonym: BRADO5992

Alternate gene names: 146342815

Gene position: 6220768-6221691 (Clockwise)

Preceding gene: 146342811

Following gene: 146342816

Centisome position: 83.43

GC content: 68.29

Gene sequence:

>924_bases
ATGCGGCGACTTCTGTTCCTGAACGGCATCAAGGCCTTCGAGGCGGCCGCCCGTACCGGCAGCTTCGCCGCCGCCGGAAC
CGAGCTCAACGTCTCCGCCGCCGCGATCAGCCGGATGGTCAACCTGCTCGAGCAGCGGCTCGGCGTCGTGCTGTTCGAGC
GCAAGGCCAACCGGCTGGCGCTGACCACGGCTGGACGCGCCTATCAGAGCGGACTGACGCCGATCTTCGACGCCCTGTCG
AGCCTGACCGCGCAGATCATGGCACCGTCGTCGATGCGGGTGCTGACGCTCGGCGTCGGCCCGACCTTTGCGATGAAATG
GCTGATCCCGCGGCTCGCCGATTTCAGGAAGGTGGAGCCCGACATCGAGGTCCGCATCACCACGGGCGGCGCGGCGGTGC
CGTTCGGCGACGACTGGAGTTGCGGCATCCGGCTCGGCGACGGCGCCTGGCCGGGCCTCGTGGCCGAACCGCTGTTCGCC
GCCGACCTCGTCCCGGTGTGCACGCCGCGGCTGGCAGCGACGCTGAAGCGGCCGACCGATCTCAAGGGTCCGAGCCTGCT
GCGCGTCTCGCATGCGGCCGAGGACTGGCCGCTATGGCTGAAGGCGGCCGGCATCACGCGCGTGACCGCGCGCGGCACCG
AATTCCAGTACTACGGCCATGCATTGCAGGCGGCGATCGACGGCCTCGGCGTCGCGATGGGCATTCGTCCCTACATCGAT
GACGATCTCGCCGCAGGACGTCTGGTTGCACCATTCGCGCTGACCGTACCCAAAGGCGAGCGCTGGTATCTGGTCTATCG
CAGCTTCCAGGCCGGACAGCGCGATTTCACCGCCTTCCGGCGCTGGATCATCCGCGCCGCCGCCCAATCCGCCACACGGA
ACGCGCCCCGCAAGGATACCGTCAAAAAAGACGCGAACGTGTGA

Upstream 100 bases:

>100_bases
GGCGTCATGGCTGATCTCCTCTCGCGGCCAACATCTCGCACTGGCGGCGGCGGCGCAAACCGAGTTATCTTGCCCTCCAT
CAGTTTTCCTGAGGGCTCGC

Downstream 100 bases:

>100_bases
ACCCGTTCACATCTGCCGAGACGTATTCATGCTGTCATTGCCGTTCGGGACATCGGGACTTGGCAATGACGTATTTGTAC
GAGGACTTCGCCATCGAGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRRLLFLNGIKAFEAAARTGSFAAAGTELNVSAAAISRMVNLLEQRLGVVLFERKANRLALTTAGRAYQSGLTPIFDALS
SLTAQIMAPSSMRVLTLGVGPTFAMKWLIPRLADFRKVEPDIEVRITTGGAAVPFGDDWSCGIRLGDGAWPGLVAEPLFA
ADLVPVCTPRLAATLKRPTDLKGPSLLRVSHAAEDWPLWLKAAGITRVTARGTEFQYYGHALQAAIDGLGVAMGIRPYID
DDLAAGRLVAPFALTVPKGERWYLVYRSFQAGQRDFTAFRRWIIRAAAQSATRNAPRKDTVKKDANV

Sequences:

>Translated_307_residues
MRRLLFLNGIKAFEAAARTGSFAAAGTELNVSAAAISRMVNLLEQRLGVVLFERKANRLALTTAGRAYQSGLTPIFDALS
SLTAQIMAPSSMRVLTLGVGPTFAMKWLIPRLADFRKVEPDIEVRITTGGAAVPFGDDWSCGIRLGDGAWPGLVAEPLFA
ADLVPVCTPRLAATLKRPTDLKGPSLLRVSHAAEDWPLWLKAAGITRVTARGTEFQYYGHALQAAIDGLGVAMGIRPYID
DDLAAGRLVAPFALTVPKGERWYLVYRSFQAGQRDFTAFRRWIIRAAAQSATRNAPRKDTVKKDANV
>Mature_307_residues
MRRLLFLNGIKAFEAAARTGSFAAAGTELNVSAAAISRMVNLLEQRLGVVLFERKANRLALTTAGRAYQSGLTPIFDALS
SLTAQIMAPSSMRVLTLGVGPTFAMKWLIPRLADFRKVEPDIEVRITTGGAAVPFGDDWSCGIRLGDGAWPGLVAEPLFA
ADLVPVCTPRLAATLKRPTDLKGPSLLRVSHAAEDWPLWLKAAGITRVTARGTEFQYYGHALQAAIDGLGVAMGIRPYID
DDLAAGRLVAPFALTVPKGERWYLVYRSFQAGQRDFTAFRRWIIRAAAQSATRNAPRKDTVKKDANV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=37.6712328767123, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1786448, Length=281, Percent_Identity=33.0960854092527, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1788706, Length=271, Percent_Identity=31.7343173431734, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=25.5102040816327, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787128, Length=256, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=25.9136212624585, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33312; Mature: 33312

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLFLNGIKAFEAAARTGSFAAAGTELNVSAAAISRMVNLLEQRLGVVLFERKANRLA
CCEEEEECCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCEEE
LTTAGRAYQSGLTPIFDALSSLTAQIMAPSSMRVLTLGVGPTFAMKWLIPRLADFRKVEP
EEECCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
DIEVRITTGGAAVPFGDDWSCGIRLGDGAWPGLVAEPLFAADLVPVCTPRLAATLKRPTD
CEEEEEEECCEECCCCCCCCCEEEECCCCCCCHHHCHHHHHHHHHHCCHHHHHHHCCCCC
LKGPSLLRVSHAAEDWPLWLKAAGITRVTARGTEFQYYGHALQAAIDGLGVAMGIRPYID
CCCCCEEEEECCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCC
DDLAAGRLVAPFALTVPKGERWYLVYRSFQAGQRDFTAFRRWIIRAAAQSATRNAPRKDT
CCCCCCCEECCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
VKKDANV
CCCCCCC
>Mature Secondary Structure
MRRLLFLNGIKAFEAAARTGSFAAAGTELNVSAAAISRMVNLLEQRLGVVLFERKANRLA
CCEEEEECCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCEEE
LTTAGRAYQSGLTPIFDALSSLTAQIMAPSSMRVLTLGVGPTFAMKWLIPRLADFRKVEP
EEECCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
DIEVRITTGGAAVPFGDDWSCGIRLGDGAWPGLVAEPLFAADLVPVCTPRLAATLKRPTD
CEEEEEEECCEECCCCCCCCCEEEECCCCCCCHHHCHHHHHHHHHHCCHHHHHHHCCCCC
LKGPSLLRVSHAAEDWPLWLKAAGITRVTARGTEFQYYGHALQAAIDGLGVAMGIRPYID
CCCCCEEEEECCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCC
DDLAAGRLVAPFALTVPKGERWYLVYRSFQAGQRDFTAFRRWIIRAAAQSATRNAPRKDT
CCCCCCCEECCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
VKKDANV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]