BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is crt [H]

Identifier: 146342811

GI number: 146342811

Start: 6218163

End: 6218945

Strand: Direct

Name: crt [H]

Synonym: BRADO5988

Alternate gene names: 146342811

Gene position: 6218163-6218945 (Clockwise)

Preceding gene: 146342809

Following gene: 146342815

Centisome position: 83.39

GC content: 70.37

Gene sequence:

>783_bases
ATGGCCGATCCCGTCCTGTGTCACGTCTCCGACCGCATCGCCCTGCTCACGCTCAACCGGCCCGAGCGGCTGAATGCGCT
GAGCTACGCGCTGATCGATGCGCTGATGGAAGCGCTCGACCGCATCGAGACCGATCCCGGCATCCGGGCCGTGATCCTCA
CCGGCACAGGCGAGCGCGCCTTTTCGGCCGGCGCCGATATCCATGAGTTCAGCGGCAGCGTCGCCGCGGGCCGCGACGCC
GCGCTGCGCGACTTCGTCCGCCGCGGGCAAACGATGACGGCGCGGCTGGAGGCGTTCCGCAAGCCGGTGATCGCCGCCGT
CAATGGCCTCGCCTTCGGCGGCGGCTGCGAGATCACCGAGGCGGTGCATCTGGCCGTCGCCAGCGATCGCGCGAGCTTCG
CAAAACCCGAGATCCGGCTCGGCATGCCGCCGACCTTCGACGGCACGCAGCGGCTGCCGCGACTGGTCGGTCGAAAGCGC
GCGCTCGAATGGCTGCTGACAGGTGATCCGTTCACCTCGCAGCGCGCGCTGGAGATCGGCCTCGTCAACGCCATCGTGCC
GCATGACGAGCTGCTGCCGGCGGCACGCGCACTCGCCCAGCGCATCATCCGCCACGGCCCCGACGCCGTCGCCAGCGTCA
TCACCGCGGCCACGCGCGGTCTCAACATGGCCATCGGCGAAGGGCTGCAGGTCGAGAGCGAACAATTCGCGCGGCTGGTC
GGCAGCCGCGATCTGGAAGATGGCCTGACAGCGTGGCGGTCGCGCCGGTCGCCTGCCGCCTAG

Upstream 100 bases:

>100_bases
ATCACTGCGCTCGACATGGGTATACTGCCCAGTGAATATACCTCGGGCAGTCCCGCGCCTAGTCTGGCTGGCAAGTCCTT
CCAGCCCGCGAGGCGCCGTC

Downstream 100 bases:

>100_bases
AGCGCGAAGCCCGGCGACTTGCGCGCCAGCCCATCGAAAGCATCGAGCAGCCGCTCGCGCCAGGCGTAGACGCTGTCGCT
CCCCTCCAGCAGGCGGAACG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MADPVLCHVSDRIALLTLNRPERLNALSYALIDALMEALDRIETDPGIRAVILTGTGERAFSAGADIHEFSGSVAAGRDA
ALRDFVRRGQTMTARLEAFRKPVIAAVNGLAFGGGCEITEAVHLAVASDRASFAKPEIRLGMPPTFDGTQRLPRLVGRKR
ALEWLLTGDPFTSQRALEIGLVNAIVPHDELLPAARALAQRIIRHGPDAVASVITAATRGLNMAIGEGLQVESEQFARLV
GSRDLEDGLTAWRSRRSPAA

Sequences:

>Translated_260_residues
MADPVLCHVSDRIALLTLNRPERLNALSYALIDALMEALDRIETDPGIRAVILTGTGERAFSAGADIHEFSGSVAAGRDA
ALRDFVRRGQTMTARLEAFRKPVIAAVNGLAFGGGCEITEAVHLAVASDRASFAKPEIRLGMPPTFDGTQRLPRLVGRKR
ALEWLLTGDPFTSQRALEIGLVNAIVPHDELLPAARALAQRIIRHGPDAVASVITAATRGLNMAIGEGLQVESEQFARLV
GSRDLEDGLTAWRSRRSPAA
>Mature_259_residues
ADPVLCHVSDRIALLTLNRPERLNALSYALIDALMEALDRIETDPGIRAVILTGTGERAFSAGADIHEFSGSVAAGRDAA
LRDFVRRGQTMTARLEAFRKPVIAAVNGLAFGGGCEITEAVHLAVASDRASFAKPEIRLGMPPTFDGTQRLPRLVGRKRA
LEWLLTGDPFTSQRALEIGLVNAIVPHDELLPAARALAQRIIRHGPDAVASVITAATRGLNMAIGEGLQVESEQFARLVG
SRDLEDGLTAWRSRRSPAA

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=38.7096774193548, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=31.4741035856574, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI68989263, Length=194, Percent_Identity=35.0515463917526, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI70995211, Length=245, Percent_Identity=29.7959183673469, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI20127408, Length=200, Percent_Identity=32, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI37594469, Length=157, Percent_Identity=33.1210191082803, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI37594471, Length=157, Percent_Identity=33.1210191082803, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=29.6, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=29.2, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=27.3504273504274, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=27.3504273504274, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=177, Percent_Identity=29.3785310734463, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=177, Percent_Identity=29.3785310734463, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=177, Percent_Identity=29.3785310734463, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI62530384, Length=191, Percent_Identity=27.7486910994764, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4757966, Length=166, Percent_Identity=27.1084337349398, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI51538581, Length=166, Percent_Identity=27.1084337349398, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI25453481, Length=166, Percent_Identity=27.1084337349398, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI51538573, Length=166, Percent_Identity=27.1084337349398, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=40.3225806451613, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=33.203125, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI221142681, Length=247, Percent_Identity=34.412955465587, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=36.5979381443299, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790281, Length=199, Percent_Identity=34.1708542713568, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=31.6602316602317, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788597, Length=210, Percent_Identity=30, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=37.3493975903614, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=36.1867704280156, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=33.6244541484716, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=193, Percent_Identity=32.6424870466321, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=189, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=33.5106382978723, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=33.5106382978723, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=249, Percent_Identity=25.3012048192771, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=197, Percent_Identity=28.4263959390863, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=197, Percent_Identity=28.4263959390863, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=27.9411764705882, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=28.75, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=210, Percent_Identity=30, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=27.906976744186, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320241, Length=205, Percent_Identity=27.8048780487805, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=38.0566801619433, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=38.0566801619433, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=31.2, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=30.1587301587302, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21357171, Length=261, Percent_Identity=28.735632183908, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28571730, Length=154, Percent_Identity=33.7662337662338, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28571729, Length=154, Percent_Identity=33.7662337662338, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.7104247104247, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583077, Length=202, Percent_Identity=30.1980198019802, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583079, Length=202, Percent_Identity=30.1980198019802, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921000, Length=201, Percent_Identity=30.3482587064677, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654903, Length=180, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650670, Length=203, Percent_Identity=31.5270935960591, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27902; Mature: 27771

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPVLCHVSDRIALLTLNRPERLNALSYALIDALMEALDRIETDPGIRAVILTGTGERA
CCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
FSAGADIHEFSGSVAAGRDAALRDFVRRGQTMTARLEAFRKPVIAAVNGLAFGGGCEITE
HHCCCCHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEECCCCCHHH
AVHLAVASDRASFAKPEIRLGMPPTFDGTQRLPRLVGRKRALEWLLTGDPFTSQRALEIG
HHHHHHHCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVNAIVPHDELLPAARALAQRIIRHGPDAVASVITAATRGLNMAIGEGLQVESEQFARLV
HHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHH
GSRDLEDGLTAWRSRRSPAA
CCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADPVLCHVSDRIALLTLNRPERLNALSYALIDALMEALDRIETDPGIRAVILTGTGERA
CCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
FSAGADIHEFSGSVAAGRDAALRDFVRRGQTMTARLEAFRKPVIAAVNGLAFGGGCEITE
HHCCCCHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEECCCCCHHH
AVHLAVASDRASFAKPEIRLGMPPTFDGTQRLPRLVGRKRALEWLLTGDPFTSQRALEIG
HHHHHHHCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVNAIVPHDELLPAARALAQRIIRHGPDAVASVITAATRGLNMAIGEGLQVESEQFARLV
HHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHH
GSRDLEDGLTAWRSRRSPAA
CCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]