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The map label for this gene is flbD [H]

Identifier: 146342733

GI number: 146342733

Start: 6121723

End: 6123096

Strand: Direct

Name: flbD [H]

Synonym: BRADO5903

Alternate gene names: 146342733

Gene position: 6121723-6123096 (Clockwise)

Preceding gene: 146342732

Following gene: 146342735

Centisome position: 82.1

GC content: 68.05

Gene sequence:

>1374_bases
ATGCGGCTTCTCATCGTCGGAACGCTGAAGGGCCAGCTCACCGCGGCCACCAAGATCGCCATGGAAAATGGCGCGACCGT
CACCCATGCCGAGGACAACGAGCAGGCGATGCGCGTGCTGCGCGGCGGCAAGGGCGCCGACCTCCTGCTCGTCGACGTCG
CGCTCGACATCCGCGACCTGGTGATGCGGCTCGATGCCGAGCACATCCATGTGCCGATCGTGGCCTGCGGCATCACCAGC
GATGCCCGCGCCGCGGTCGCCGCGATCCATGCCGGCGCCAAGGAATACATCCCGCTGCCGCCCGACCCCGAGCTGATCGC
CGCGGTTCTCGCCGCCGTCGCCAACGACAGCCGCGACCTCGTCTATCGCGACGACGCGATGGCCAAGGTGATCAAGCTCG
CCCAGCAGATCGCGGGCTCCGACGCCTCGGTGATGATCACCGGCGAGTCCGGCACGGGCAAGGAGGTGCTGGCGCGCTAC
GTCCACACCCGCTCGGCCCGCGCCAAGCGCCCGTTCATCTCGATCAACTGCGCCGCGATCCCCGAGCATCTGCTGGAGTC
CGAGCTGTTCGGCCACGAGAAGGGCGCCTTCACCGGCGCCGTCGCGCGCCGCATCGGCAAGTTCGAGGAGGCCAATGGCG
GCACGCTGCTGCTCGACGAAATCTCCGAGATGGACGTGCGGCTGCAGTCCAAGCTGCTGCGCGCGATCCAGGAGCGCGTG
ATCGATCGCGTCGGCGGCACCCGCCCCGTCGCGGTCGACATCCGCATCATCGCGACCTCGAACCGCAACCTTGCCGATGC
CGTGCGCGAAGGCACGTTCCGCGAGGACCTGCTGTTCCGCCTCAACGTCGTCAACCTGAAGATCCCTCCCCTGCGCGAGC
GTCCCGCTGACATCCTCGAGCTGGCGCAGCATTTCGCGCGCAAATATTCCGAGGCTAACGCCGTGCCGCTGCGACCGATC
TCGGCGGAGGCCAAGCGTATTCTTACGACCAACCGCTGGCCGGGCAATGTGCGCGAGCTCGAGAACACCATGCATCGCGC
GGTGCTGATGGCGCAGGGCGACGAGATCGGGCCCGACGCGATCCTCACTCCCGACGGCGCCCGCCTCGACATCGGCAAGA
CCCAGCCGGCCGTGGCTCATGCTACGATGGCGGCGGAGCAGGTCACGCGCGCCCTCGTCGGCCGCACCGTCGCCGACGTC
GAGCGCGACCTGATCCTGGAAACCTTGAAGCACTGCCTGGGCAACCGGACACATGCGGCCAACATTCTGGGCATCTCGAT
CCGCACGTTGCGCAACAAGCTCAACGAATACGCCGACGGCGGCGTGCCGATCCCGCCACCTGGCGCCGGCGAGCGGATGC
CGAGTTCTGTCTGA

Upstream 100 bases:

>100_bases
AGCTCGGCGTGACGATGACGGAAATCATCAAGTCCGACCGCAACTGACAGACATGACCGGCGCGCTGATCGAGAGCGGCG
CGATCGTATAGGAGACCACC

Downstream 100 bases:

>100_bases
ACAGCGAGAATTGGTTGCTCCGACTCAACTCGGCGCGCGCCGGTGTCGGCGCGCCGTTGCCATTTGCCAGACGGCGAAAC
GCGTGAGTTGAACCGACGAG

Product: two component, sigma-54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRLLIVGTLKGQLTAATKIAMENGATVTHAEDNEQAMRVLRGGKGADLLLVDVALDIRDLVMRLDAEHIHVPIVACGITS
DARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRDLVYRDDAMAKVIKLAQQIAGSDASVMITGESGTGKEVLARY
VHTRSARAKRPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDVRLQSKLLRAIQERV
IDRVGGTRPVAVDIRIIATSNRNLADAVREGTFREDLLFRLNVVNLKIPPLRERPADILELAQHFARKYSEANAVPLRPI
SAEAKRILTTNRWPGNVRELENTMHRAVLMAQGDEIGPDAILTPDGARLDIGKTQPAVAHATMAAEQVTRALVGRTVADV
ERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADGGVPIPPPGAGERMPSSV

Sequences:

>Translated_457_residues
MRLLIVGTLKGQLTAATKIAMENGATVTHAEDNEQAMRVLRGGKGADLLLVDVALDIRDLVMRLDAEHIHVPIVACGITS
DARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRDLVYRDDAMAKVIKLAQQIAGSDASVMITGESGTGKEVLARY
VHTRSARAKRPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDVRLQSKLLRAIQERV
IDRVGGTRPVAVDIRIIATSNRNLADAVREGTFREDLLFRLNVVNLKIPPLRERPADILELAQHFARKYSEANAVPLRPI
SAEAKRILTTNRWPGNVRELENTMHRAVLMAQGDEIGPDAILTPDGARLDIGKTQPAVAHATMAAEQVTRALVGRTVADV
ERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADGGVPIPPPGAGERMPSSV
>Mature_457_residues
MRLLIVGTLKGQLTAATKIAMENGATVTHAEDNEQAMRVLRGGKGADLLLVDVALDIRDLVMRLDAEHIHVPIVACGITS
DARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRDLVYRDDAMAKVIKLAQQIAGSDASVMITGESGTGKEVLARY
VHTRSARAKRPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDVRLQSKLLRAIQERV
IDRVGGTRPVAVDIRIIATSNRNLADAVREGTFREDLLFRLNVVNLKIPPLRERPADILELAQHFARKYSEANAVPLRPI
SAEAKRILTTNRWPGNVRELENTMHRAVLMAQGDEIGPDAILTPDGARLDIGKTQPAVAHATMAAEQVTRALVGRTVADV
ERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADGGVPIPPPGAGERMPSSV

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=432, Percent_Identity=42.3611111111111, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790299, Length=442, Percent_Identity=39.5927601809955, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1790437, Length=436, Percent_Identity=39.4495412844037, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI87082117, Length=239, Percent_Identity=51.0460251046025, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788905, Length=436, Percent_Identity=37.6146788990826, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1789233, Length=313, Percent_Identity=44.408945686901, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1789087, Length=256, Percent_Identity=46.484375, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=38.9776357827476, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=47.8813559322034, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=37.6899696048632, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=330, Percent_Identity=39.3939393939394, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=33.0909090909091, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081858, Length=347, Percent_Identity=28.2420749279539, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49374; Mature: 49374

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLIVGTLKGQLTAATKIAMENGATVTHAEDNEQAMRVLRGGKGADLLLVDVALDIRDL
CEEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHH
VMRLDAEHIHVPIVACGITSDARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRDL
HHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCE
VYRDDAMAKVIKLAQQIAGSDASVMITGESGTGKEVLARYVHTRSARAKRPFISINCAAI
EECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHH
PEHLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDVRLQSKLLRAIQERV
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
IDRVGGTRPVAVDIRIIATSNRNLADAVREGTFREDLLFRLNVVNLKIPPLRERPADILE
HHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHEEEEEEEEECCCCCCCCHHHHH
LAQHFARKYSEANAVPLRPISAEAKRILTTNRWPGNVRELENTMHRAVLMAQGDEIGPDA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
ILTPDGARLDIGKTQPAVAHATMAAEQVTRALVGRTVADVERDLILETLKHCLGNRTHAA
EECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NILGISIRTLRNKLNEYADGGVPIPPPGAGERMPSSV
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLLIVGTLKGQLTAATKIAMENGATVTHAEDNEQAMRVLRGGKGADLLLVDVALDIRDL
CEEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEHHHHHHH
VMRLDAEHIHVPIVACGITSDARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRDL
HHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCE
VYRDDAMAKVIKLAQQIAGSDASVMITGESGTGKEVLARYVHTRSARAKRPFISINCAAI
EECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHH
PEHLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDVRLQSKLLRAIQERV
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
IDRVGGTRPVAVDIRIIATSNRNLADAVREGTFREDLLFRLNVVNLKIPPLRERPADILE
HHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHEEEEEEEEECCCCCCCCHHHHH
LAQHFARKYSEANAVPLRPISAEAKRILTTNRWPGNVRELENTMHRAVLMAQGDEIGPDA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
ILTPDGARLDIGKTQPAVAHATMAAEQVTRALVGRTVADVERDLILETLKHCLGNRTHAA
EECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NILGISIRTLRNKLNEYADGGVPIPPPGAGERMPSSV
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]