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The map label for this gene is glnA [H]

Identifier: 146342715

GI number: 146342715

Start: 6102589

End: 6103887

Strand: Reverse

Name: glnA [H]

Synonym: BRADO5882

Alternate gene names: 146342715

Gene position: 6103887-6102589 (Counterclockwise)

Preceding gene: 146342716

Following gene: 146342710

Centisome position: 81.86

GC content: 62.89

Gene sequence:

>1299_bases
ATGTCGAGTAGTCTCCATGCCGTCGCGTGCGAACGAGGCATAAAATATTTCCTGATCTCCTACACCGACCTGTTCGGCGT
GCAGCGCGCCAAGCTCGTGCCGGCTGCGGCAATCGACGGCATGGTCAAGACCGGCGCTGGTTTCGCCGGCTTCGCGACCT
GGCTCGACATGACGCCGGCGGATCCCGACGTGTTCGCGATGCCGGATCCGGCGAGCCTGATCCAGCTGCCATGGAAGCCG
GAAGTCGGCTGGCTCGCCGCCGATCCTTGGATGAACGGCAAGCCCGTGGCCCACGCGCCACGCAACCTGCTCAAGCACAT
GATCGCCGATGCGGCGGCGCAAGGCTATGAGCTGAAATCGGGCGTCGAATGCGAGTTCTTCCTGATCTCGCCCGACGGCA
CCGAGCTGTCCGATGCGGCGGACCGGCAATCGAAGCCCTGCTACGACCAGCAGGCACTGATGCGCCGATACGAGGTGATC
GCCGAAATCTGCGACGCCATGCTGGCGCTCGGCTGGAAGCCGTATCAGAACGATCACGAGGATGCCAACGGCCAGTTCGA
GATGAACTGGGAGTTCGACACGGCGCTGAAGACGGCCGACCGTCACGCGTTCTTCAAATACATGGTGCGCTCGATCGCCG
AGAAGCACGGGCTGCGTGCCACCTTCATGCCGAAGCCGTTCGTTGATCTCACCGGCAATGGCTGCCATGCTCACGTCTCG
GTATGGAAGGACGGCCGCAATCTGTTCGAGGACGAGGCCGGCGAGCTCGGCGTGTCCAAGCTGGGCTATCAATTCCTCGG
CGGCATCATGCATTCCGCTGATGCGCTCGCCGCGCTCTTCAACCCCACGGTCAACTCATACAAACGCATCAATGCGGCGC
GCACGACATCGGGCGCGACCTGGGCGCCGAACTCAGTGACCTATTCCGGCAACAACCGGACCCATCTGATCCGCATTCCG
GAAGCCGGCCGCTTCGAGGTCCGTCTCGCCGATGGCGCCGCCAATCCGTATCTGCTGCAGGCCGGGCTGCTCGCTGCGGG
TCTCGACGGCATCGCCCATGAGCGTCATCCGGGCACGCGTCTCGACATCAACATGTACACCGATGGCCATACGGTGACGA
CCGGCAAGAAGCTGCCGCTCAATCTGCTGGACGCCATCCGCGCGTTGGAAGCGTCGACTGTGCTCGGCGAGAAGCTCGGC
GATTTCATTCCCTGCTATGTCAAGCTCAAGACCGAGGAATGGAATGCCTATGCGCGCCATCTGACGCAGTGGGAGCGCGA
CACGACGCTGGATTGCTGA

Upstream 100 bases:

>100_bases
TTCCGGGGCTCGACAAATAGGCTGGCAGTGCTGCGCGCGCCGCTGACGAGATCGACGGATAGAAGATGCCGGGGTCTTCT
GAGGACTCAAAGGGGAAACC

Downstream 100 bases:

>100_bases
GCGGTCGATGAGGCCGGTGACGTGAGCCCTGTCGTCACCGCGCGTCAACGCGAGGAGCGCAGCGACGACGCAATCCGGAG
TCCCGCACGCGCCCCCGGGA

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSSSLHAVACERGIKYFLISYTDLFGVQRAKLVPAAAIDGMVKTGAGFAGFATWLDMTPADPDVFAMPDPASLIQLPWKP
EVGWLAADPWMNGKPVAHAPRNLLKHMIADAAAQGYELKSGVECEFFLISPDGTELSDAADRQSKPCYDQQALMRRYEVI
AEICDAMLALGWKPYQNDHEDANGQFEMNWEFDTALKTADRHAFFKYMVRSIAEKHGLRATFMPKPFVDLTGNGCHAHVS
VWKDGRNLFEDEAGELGVSKLGYQFLGGIMHSADALAALFNPTVNSYKRINAARTTSGATWAPNSVTYSGNNRTHLIRIP
EAGRFEVRLADGAANPYLLQAGLLAAGLDGIAHERHPGTRLDINMYTDGHTVTTGKKLPLNLLDAIRALEASTVLGEKLG
DFIPCYVKLKTEEWNAYARHLTQWERDTTLDC

Sequences:

>Translated_432_residues
MSSSLHAVACERGIKYFLISYTDLFGVQRAKLVPAAAIDGMVKTGAGFAGFATWLDMTPADPDVFAMPDPASLIQLPWKP
EVGWLAADPWMNGKPVAHAPRNLLKHMIADAAAQGYELKSGVECEFFLISPDGTELSDAADRQSKPCYDQQALMRRYEVI
AEICDAMLALGWKPYQNDHEDANGQFEMNWEFDTALKTADRHAFFKYMVRSIAEKHGLRATFMPKPFVDLTGNGCHAHVS
VWKDGRNLFEDEAGELGVSKLGYQFLGGIMHSADALAALFNPTVNSYKRINAARTTSGATWAPNSVTYSGNNRTHLIRIP
EAGRFEVRLADGAANPYLLQAGLLAAGLDGIAHERHPGTRLDINMYTDGHTVTTGKKLPLNLLDAIRALEASTVLGEKLG
DFIPCYVKLKTEEWNAYARHLTQWERDTTLDC
>Mature_431_residues
SSSLHAVACERGIKYFLISYTDLFGVQRAKLVPAAAIDGMVKTGAGFAGFATWLDMTPADPDVFAMPDPASLIQLPWKPE
VGWLAADPWMNGKPVAHAPRNLLKHMIADAAAQGYELKSGVECEFFLISPDGTELSDAADRQSKPCYDQQALMRRYEVIA
EICDAMLALGWKPYQNDHEDANGQFEMNWEFDTALKTADRHAFFKYMVRSIAEKHGLRATFMPKPFVDLTGNGCHAHVSV
WKDGRNLFEDEAGELGVSKLGYQFLGGIMHSADALAALFNPTVNSYKRINAARTTSGATWAPNSVTYSGNNRTHLIRIPE
AGRFEVRLADGAANPYLLQAGLLAAGLDGIAHERHPGTRLDINMYTDGHTVTTGKKLPLNLLDAIRALEASTVLGEKLGD
FIPCYVKLKTEEWNAYARHLTQWERDTTLDC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=428, Percent_Identity=24.7663551401869, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1790301, Length=274, Percent_Identity=39.051094890511, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081870, Length=444, Percent_Identity=28.3783783783784, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 47643; Mature: 47511

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSLHAVACERGIKYFLISYTDLFGVQRAKLVPAAAIDGMVKTGAGFAGFATWLDMTPA
CCCCHHHHHHHCCCEEEEEEHHHHHCCHHHHCCCHHHHCCHHHCCCCHHHHHHHHCCCCC
DPDVFAMPDPASLIQLPWKPEVGWLAADPWMNGKPVAHAPRNLLKHMIADAAAQGYELKS
CCCEEECCCCHHHEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GVECEFFLISPDGTELSDAADRQSKPCYDQQALMRRYEVIAEICDAMLALGWKPYQNDHE
CCEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DANGQFEMNWEFDTALKTADRHAFFKYMVRSIAEKHGLRATFMPKPFVDLTGNGCHAHVS
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCEEEEE
VWKDGRNLFEDEAGELGVSKLGYQFLGGIMHSADALAALFNPTVNSYKRINAARTTSGAT
EECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
WAPNSVTYSGNNRTHLIRIPEAGRFEVRLADGAANPYLLQAGLLAAGLDGIAHERHPGTR
CCCCCEEECCCCEEEEEECCCCCCEEEEEECCCCCCCEEHHHHHHHCCHHHHHCCCCCCE
LDINMYTDGHTVTTGKKLPLNLLDAIRALEASTVLGEKLGDFIPCYVKLKTEEWNAYARH
EEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
LTQWERDTTLDC
HHHHCCCCCCCC
>Mature Secondary Structure 
SSSLHAVACERGIKYFLISYTDLFGVQRAKLVPAAAIDGMVKTGAGFAGFATWLDMTPA
CCCHHHHHHHCCCEEEEEEHHHHHCCHHHHCCCHHHHCCHHHCCCCHHHHHHHHCCCCC
DPDVFAMPDPASLIQLPWKPEVGWLAADPWMNGKPVAHAPRNLLKHMIADAAAQGYELKS
CCCEEECCCCHHHEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GVECEFFLISPDGTELSDAADRQSKPCYDQQALMRRYEVIAEICDAMLALGWKPYQNDHE
CCEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DANGQFEMNWEFDTALKTADRHAFFKYMVRSIAEKHGLRATFMPKPFVDLTGNGCHAHVS
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCEEEEE
VWKDGRNLFEDEAGELGVSKLGYQFLGGIMHSADALAALFNPTVNSYKRINAARTTSGAT
EECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
WAPNSVTYSGNNRTHLIRIPEAGRFEVRLADGAANPYLLQAGLLAAGLDGIAHERHPGTR
CCCCCEEECCCCEEEEEECCCCCCEEEEEECCCCCCCEEHHHHHHHCCHHHHHCCCCCCE
LDINMYTDGHTVTTGKKLPLNLLDAIRALEASTVLGEKLGDFIPCYVKLKTEEWNAYARH
EEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
LTQWERDTTLDC
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]