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The map label for this gene is amiE [H]

Identifier: 146342702

GI number: 146342702

Start: 6088140

End: 6089177

Strand: Reverse

Name: amiE [H]

Synonym: BRADO5869

Alternate gene names: 146342702

Gene position: 6089177-6088140 (Counterclockwise)

Preceding gene: 146342703

Following gene: 146342701

Centisome position: 81.66

GC content: 60.6

Gene sequence:

>1038_bases
ATGCTGCACGGTGATATCACTTCGAGTAACGACACGATCGGCGTCGCCGTCGTGAACTACAAAATGCCGCGCCTCCACAC
CAAGGCGGAGGTGCTCGACAATGCCCGCAAGATCGCCGACATGATCGTCGGCATGAAGACGGGTCTGCCGGGCATGGACC
TCGTGATCTTCCCGGAATACTCGACCCACGGCATCATGTACGACTCCAAGGAGATGTATGAGACGGCCTCGACCGTGCCG
GGCGACGAGACCGAGATCTTTGCGGAAGCCTGCCGAAAGGCGAAGGTCTGGGGCGTGTTTTCGCTGACCGGTGAGCGCCA
CGAGGAACACCCGAAGAAGGCGCCCTACAACACGCTGATCCTGATGAACGACAAGGGCGAGATCGTGCAGAAATATCGCA
AGATCATGCCCTGGGTGCCGATCGAGGGCTGGTATCCCGGCAACTGCACCTATGTCTCGGAAGGGCCGAAGGGCCTGAAA
ATCTCGCTCATCATCTGCGACGACGGCAATTATCCCGAGATCTGGCGTGACTGCGCCATGAAGGGCGCCGAGCTGATCGT
GCGCTGCCAGGGCTACATGTATCCGGCCAAGGATCAGCAGGTGATCATGGCAAAGGCGATGGCCTGGGCCAACAACGTCT
ATGTCGCGGTTGCCAATGCGTCCGGCTTCGACGGCGTCTACTCCTATTTCGGCCATTCGGCGATCATCGGATTCGATGGC
CGTACGCTTGGCGAATGCGGCGAGGAGGATTACGGCGTTCAGTACGCGCAGCTGTCCAAGTCGCTGATCCGCGACGCGCG
CAAGAACGGCCAGTCGCAGAATCACCTCTTCAAGCTGCTGCACCGCGGCTACACCGGCATGATCAACTCCGGCGATGGCG
ATCGCGGCGTCGCGGCCTGTCCGTATGATTTCTACGCCAAGTGGGTCGCCGATCCCGAAGGAACGCGCGAGATGGTGGAG
TCGTTCACCCGCTCCACGGTCGGGACCGAGGAATGTCCGATCGAGGGCATTCCGAACAAGAAGGTCGCGCACCGGTAG

Upstream 100 bases:

>100_bases
CGGTGTGACGGGTCTGCGTCGTGGAGAAAGGGAGGTGCGTCGACCAGCGCACCGGATGTGTAACGGCAGGCTTTGACGGG
TTGGTAGCAAGGAGAGCAAG

Downstream 100 bases:

>100_bases
GCGGGGCCAAGCGAGAGCGGCGGAGCTGTCCGCCGCTCTCCTGATCCGCCGGACCTTCCGCTTGGGGTAGGCAGGGCCGG
ACGCGGCAGGTCGAGGCGCA

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLHGDITSSNDTIGVAVVNYKMPRLHTKAEVLDNARKIADMIVGMKTGLPGMDLVIFPEYSTHGIMYDSKEMYETASTVP
GDETEIFAEACRKAKVWGVFSLTGERHEEHPKKAPYNTLILMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLK
ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVIMAKAMAWANNVYVAVANASGFDGVYSYFGHSAIIGFDG
RTLGECGEEDYGVQYAQLSKSLIRDARKNGQSQNHLFKLLHRGYTGMINSGDGDRGVAACPYDFYAKWVADPEGTREMVE
SFTRSTVGTEECPIEGIPNKKVAHR

Sequences:

>Translated_345_residues
MLHGDITSSNDTIGVAVVNYKMPRLHTKAEVLDNARKIADMIVGMKTGLPGMDLVIFPEYSTHGIMYDSKEMYETASTVP
GDETEIFAEACRKAKVWGVFSLTGERHEEHPKKAPYNTLILMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLK
ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVIMAKAMAWANNVYVAVANASGFDGVYSYFGHSAIIGFDG
RTLGECGEEDYGVQYAQLSKSLIRDARKNGQSQNHLFKLLHRGYTGMINSGDGDRGVAACPYDFYAKWVADPEGTREMVE
SFTRSTVGTEECPIEGIPNKKVAHR
>Mature_345_residues
MLHGDITSSNDTIGVAVVNYKMPRLHTKAEVLDNARKIADMIVGMKTGLPGMDLVIFPEYSTHGIMYDSKEMYETASTVP
GDETEIFAEACRKAKVWGVFSLTGERHEEHPKKAPYNTLILMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLK
ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVIMAKAMAWANNVYVAVANASGFDGVYSYFGHSAIIGFDG
RTLGECGEEDYGVQYAQLSKSLIRDARKNGQSQNHLFKLLHRGYTGMINSGDGDRGVAACPYDFYAKWVADPEGTREMVE
SFTRSTVGTEECPIEGIPNKKVAHR

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 38419; Mature: 38419

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGDITSSNDTIGVAVVNYKMPRLHTKAEVLDNARKIADMIVGMKTGLPGMDLVIFPEY
CCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
STHGIMYDSKEMYETASTVPGDETEIFAEACRKAKVWGVFSLTGERHEEHPKKAPYNTLI
CCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEECCCHHHCCCCCCCCCEEE
LMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLKISLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCEECCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHH
KGAELIVRCQGYMYPAKDQQVIMAKAMAWANNVYVAVANASGFDGVYSYFGHSAIIGFDG
CCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEEEECCC
RTLGECGEEDYGVQYAQLSKSLIRDARKNGQSQNHLFKLLHRGYTGMINSGDGDRGVAAC
CCHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEC
PYDFYAKWVADPEGTREMVESFTRSTVGTEECPIEGIPNKKVAHR
CHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLHGDITSSNDTIGVAVVNYKMPRLHTKAEVLDNARKIADMIVGMKTGLPGMDLVIFPEY
CCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
STHGIMYDSKEMYETASTVPGDETEIFAEACRKAKVWGVFSLTGERHEEHPKKAPYNTLI
CCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEECCCHHHCCCCCCCCCEEE
LMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLKISLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCEECCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHH
KGAELIVRCQGYMYPAKDQQVIMAKAMAWANNVYVAVANASGFDGVYSYFGHSAIIGFDG
CCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEEEECCC
RTLGECGEEDYGVQYAQLSKSLIRDARKNGQSQNHLFKLLHRGYTGMINSGDGDRGVAAC
CCHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEC
PYDFYAKWVADPEGTREMVESFTRSTVGTEECPIEGIPNKKVAHR
CHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA