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The map label for this gene is plcN [H]

Identifier: 146342591

GI number: 146342591

Start: 5952667

End: 5954796

Strand: Reverse

Name: plcN [H]

Synonym: BRADO5754

Alternate gene names: 146342591

Gene position: 5954796-5952667 (Counterclockwise)

Preceding gene: 146342594

Following gene: 146342589

Centisome position: 79.86

GC content: 65.07

Gene sequence:

>2130_bases
ATGTCGAACACGATCGCGCATAACCGGCGTGAGTTCCTGCGCCTGCTCGGAGCCTCGGCTGCGACGGCTGCGCTCAGCCA
GAGCATCGAGCGCGCGCATGCCATTCCGGCGTTTAATCGCCATGGCAGCATCGAGGACGTCAAGCACATCGTCGTGCTGA
TGCAGGAGAACCGCGCCTTCGATCATTATTTCGGCGCGATGCGTGGCGTCCGCGGCTTCAACGACCCGCGCGCCGTCAAG
CTGCCGAACGGCAATCCGGTCTGGCAGCAGCCGAACGGTTCGGGATCGGTGATGCCGTTCCGTGTCGACAATGCCGGCCA
GGTCTATGTCGAGGATGTCGCGCATGGCTGGAACGACGGCCAGAAGTCCTGGAACAACGGCAATTACGACAAGTGGATCG
CCAACAAGGGCACCACAACGATGACCTGCATGAACCGGCAGGATCTGCCCTGGCACTACGCGCTCGCCGACGCCTTCACG
ATCTGCGACCAGTATTTCTGCTCGGTGATGGGACCGACCGACCCGAACCGCTATTATCTGTGGACCGGCTGGGACGGCAA
TGACGGCAAGAACGGCGGCCCGGTCATCACCAACGCCGAGGCCGGCTACGACTGGACCACGTTCCCGGAGCGGCTGGAAA
AAGCCGGCATCTCCTGGAAGGTCTATCAGGACATCGGCGTCGGCCTCACCGCTGCAGGCTACTGGGGCTGGACCGAGAAT
CCGTGGATCGGCAATTACGGCGACAACTCGCTGCTCTACTTCCACCAGTACCAGAACTCGCAGCCGGGCAGCCCGCTGTA
TGAGAAGGCGCTGCGCGGCAGCAACATCGCCAAGGGCGGCACGTTCGAGAACCTGTTCGATGGCCTGCGCAACGACGTCC
GCAACGGGACCCTGCCTCAGGTGTCGTGGATCGCGGCTCCGGAAGCGTTCAGCGAGCATCCGAACTGGCTGCCGGGTCCG
GGCGCCTGGTACATCTCCAAGGTGCTGGACATCCTGACCTCGAATCCGGAGCTGTGGAGCAAGACCGTTCTGCTCATCAA
CTATGACGAGGGTGGCGGCTTCTTCGATCACGTCGTCGGCCCGTATCCGGCGATGTCGCAGGCGTGGGGTCAGTCGACGG
TCGACGTCAGCACCGACCTGTTCGCCGGCGACGCCAACCACGTCGCGGGTCCCTATGGCCTCGGCGTGCGCGTGCCGATG
ATGGTGGTGTCGCCGTGGTCGCGCGGCGGCTATGTGTGCTCGCAGGTGTTCGACCACACCTCGGTGATCCGCTTCATCGA
GCAGCGCTTCCATCGCCACGCGAAGGATCTGTTCGAGACCAATATCCCGGCCTGGCGTCGCGCGGTGTGTGGCGATCTCA
CCTCGGCGTTCAACTTCACCTCGCCGAACGAAGGCATGGCGCCGCTGCCGAGCACGGCCGGCTTTAAGCCGCCGGTCGCC
GATATCACCTCGGGGGCACGCTTCGACGACTATCATCCGGTGCCGCCGGCCAAGCAGGTCCTGCCGCAGCAGGAGCCCGG
CATCCGCCCGGCGCGCGCACTGCCTTACGATCTGCGCGTCGACGGCGATGCCGATGCCTCCAACAAGAAGTTCGGCATCC
GCTTCGTCAATCCGGGGCAGGCGGGCGTCTGCTTCCACGTCCGTTCCGGCAACACCGCAACCGGCCCGTGGAGCTACACG
GTCGAGGCCGGCAAGTCGCTGAACGGCGTGTGGGACCTCGCGGCCAGCGCCGGCCAGTATGATCTGTCGGTGTACGGCCC
GAACGGCTTCTACCGGCACTTCAAGGGCGGCGTCGTCTCGACGGCGGCATTGATCGACGTCGACACCGTGCATCTCTGCG
ACCGGGAGGAAGGCGACGACGATCGCAGCCTCGCGATTGCGCTAACCAACAAGGGCGCGGGCTGCGTGGTGACGATCGCC
GACAACTACACCGGCCGCAGCGAGCGGCATCGCCTGCAGCGCGGCCAGCGCCTGGAGACGCAGGTTCGCCTGCGCCAGAC
CCATGGCTGGTACGACCTGACGGTCACGACCGACACCGATGCCAATTTCAACTGGCAGCTCGCCGGTCATGTCGAGAACG
GCCGTCCCAGCATCAGCGATCCCGCGATGGGCAAGATCGCCGCGACCTGA

Upstream 100 bases:

>100_bases
TGTCAAACCCGTGCCACACATCCTTAACCGCCCGGTCACGCCCCTGTTAACGCAGCGACCTAAGTCGCTCGACACTGTCA
TCACTTGGGGAGTGAGAGAG

Downstream 100 bases:

>100_bases
TCGCGGACTTTACCAACGCGATGCGCCCGGAGCGATCCGGGCGCATCGTCAAGACGAAGAGGTGACGAGCGATTGATCGG
CGTCGTCGGCGTTGGGATCA

Product: non-hemolytic phospholipase C

Products: NA

Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]

Number of amino acids: Translated: 709; Mature: 708

Protein sequence:

>709_residues
MSNTIAHNRREFLRLLGASAATAALSQSIERAHAIPAFNRHGSIEDVKHIVVLMQENRAFDHYFGAMRGVRGFNDPRAVK
LPNGNPVWQQPNGSGSVMPFRVDNAGQVYVEDVAHGWNDGQKSWNNGNYDKWIANKGTTTMTCMNRQDLPWHYALADAFT
ICDQYFCSVMGPTDPNRYYLWTGWDGNDGKNGGPVITNAEAGYDWTTFPERLEKAGISWKVYQDIGVGLTAAGYWGWTEN
PWIGNYGDNSLLYFHQYQNSQPGSPLYEKALRGSNIAKGGTFENLFDGLRNDVRNGTLPQVSWIAAPEAFSEHPNWLPGP
GAWYISKVLDILTSNPELWSKTVLLINYDEGGGFFDHVVGPYPAMSQAWGQSTVDVSTDLFAGDANHVAGPYGLGVRVPM
MVVSPWSRGGYVCSQVFDHTSVIRFIEQRFHRHAKDLFETNIPAWRRAVCGDLTSAFNFTSPNEGMAPLPSTAGFKPPVA
DITSGARFDDYHPVPPAKQVLPQQEPGIRPARALPYDLRVDGDADASNKKFGIRFVNPGQAGVCFHVRSGNTATGPWSYT
VEAGKSLNGVWDLAASAGQYDLSVYGPNGFYRHFKGGVVSTAALIDVDTVHLCDREEGDDDRSLAIALTNKGAGCVVTIA
DNYTGRSERHRLQRGQRLETQVRLRQTHGWYDLTVTTDTDANFNWQLAGHVENGRPSISDPAMGKIAAT

Sequences:

>Translated_709_residues
MSNTIAHNRREFLRLLGASAATAALSQSIERAHAIPAFNRHGSIEDVKHIVVLMQENRAFDHYFGAMRGVRGFNDPRAVK
LPNGNPVWQQPNGSGSVMPFRVDNAGQVYVEDVAHGWNDGQKSWNNGNYDKWIANKGTTTMTCMNRQDLPWHYALADAFT
ICDQYFCSVMGPTDPNRYYLWTGWDGNDGKNGGPVITNAEAGYDWTTFPERLEKAGISWKVYQDIGVGLTAAGYWGWTEN
PWIGNYGDNSLLYFHQYQNSQPGSPLYEKALRGSNIAKGGTFENLFDGLRNDVRNGTLPQVSWIAAPEAFSEHPNWLPGP
GAWYISKVLDILTSNPELWSKTVLLINYDEGGGFFDHVVGPYPAMSQAWGQSTVDVSTDLFAGDANHVAGPYGLGVRVPM
MVVSPWSRGGYVCSQVFDHTSVIRFIEQRFHRHAKDLFETNIPAWRRAVCGDLTSAFNFTSPNEGMAPLPSTAGFKPPVA
DITSGARFDDYHPVPPAKQVLPQQEPGIRPARALPYDLRVDGDADASNKKFGIRFVNPGQAGVCFHVRSGNTATGPWSYT
VEAGKSLNGVWDLAASAGQYDLSVYGPNGFYRHFKGGVVSTAALIDVDTVHLCDREEGDDDRSLAIALTNKGAGCVVTIA
DNYTGRSERHRLQRGQRLETQVRLRQTHGWYDLTVTTDTDANFNWQLAGHVENGRPSISDPAMGKIAAT
>Mature_708_residues
SNTIAHNRREFLRLLGASAATAALSQSIERAHAIPAFNRHGSIEDVKHIVVLMQENRAFDHYFGAMRGVRGFNDPRAVKL
PNGNPVWQQPNGSGSVMPFRVDNAGQVYVEDVAHGWNDGQKSWNNGNYDKWIANKGTTTMTCMNRQDLPWHYALADAFTI
CDQYFCSVMGPTDPNRYYLWTGWDGNDGKNGGPVITNAEAGYDWTTFPERLEKAGISWKVYQDIGVGLTAAGYWGWTENP
WIGNYGDNSLLYFHQYQNSQPGSPLYEKALRGSNIAKGGTFENLFDGLRNDVRNGTLPQVSWIAAPEAFSEHPNWLPGPG
AWYISKVLDILTSNPELWSKTVLLINYDEGGGFFDHVVGPYPAMSQAWGQSTVDVSTDLFAGDANHVAGPYGLGVRVPMM
VVSPWSRGGYVCSQVFDHTSVIRFIEQRFHRHAKDLFETNIPAWRRAVCGDLTSAFNFTSPNEGMAPLPSTAGFKPPVAD
ITSGARFDDYHPVPPAKQVLPQQEPGIRPARALPYDLRVDGDADASNKKFGIRFVNPGQAGVCFHVRSGNTATGPWSYTV
EAGKSLNGVWDLAASAGQYDLSVYGPNGFYRHFKGGVVSTAALIDVDTVHLCDREEGDDDRSLAIALTNKGAGCVVTIAD
NYTGRSERHRLQRGQRLETQVRLRQTHGWYDLTVTTDTDANFNWQLAGHVENGRPSISDPAMGKIAAT

Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR008475
- InterPro:   IPR017767
- InterPro:   IPR006311 [H]

Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 78154; Mature: 78022

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTIAHNRREFLRLLGASAATAALSQSIERAHAIPAFNRHGSIEDVKHIVVLMQENRAF
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCCH
DHYFGAMRGVRGFNDPRAVKLPNGNPVWQQPNGSGSVMPFRVDNAGQVYVEDVAHGWNDG
HHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCC
QKSWNNGNYDKWIANKGTTTMTCMNRQDLPWHYALADAFTICDQYFCSVMGPTDPNRYYL
CCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
WTGWDGNDGKNGGPVITNAEAGYDWTTFPERLEKAGISWKVYQDIGVGLTAAGYWGWTEN
EECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEEEEECCCCCC
PWIGNYGDNSLLYFHQYQNSQPGSPLYEKALRGSNIAKGGTFENLFDGLRNDVRNGTLPQ
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
VSWIAAPEAFSEHPNWLPGPGAWYISKVLDILTSNPELWSKTVLLINYDEGGGFFDHVVG
EEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHCC
PYPAMSQAWGQSTVDVSTDLFAGDANHVAGPYGLGVRVPMMVVSPWSRGGYVCSQVFDHT
CCCCHHHHCCCCEEECCCCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
SVIRFIEQRFHRHAKDLFETNIPAWRRAVCGDLTSAFNFTSPNEGMAPLPSTAGFKPPVA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
DITSGARFDDYHPVPPAKQVLPQQEPGIRPARALPYDLRVDGDADASNKKFGIRFVNPGQ
HHCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHCCCEEEEECCCCCCCCCEEEEEEECCCC
AGVCFHVRSGNTATGPWSYTVEAGKSLNGVWDLAASAGQYDLSVYGPNGFYRHFKGGVVS
CEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHCCCCEE
TAALIDVDTVHLCDREEGDDDRSLAIALTNKGAGCVVTIADNYTGRSERHRLQRGQRLET
EEEEEECCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCHHHH
QVRLRQTHGWYDLTVTTDTDANFNWQLAGHVENGRPSISDPAMGKIAAT
HHHHHHCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
SNTIAHNRREFLRLLGASAATAALSQSIERAHAIPAFNRHGSIEDVKHIVVLMQENRAF
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCCH
DHYFGAMRGVRGFNDPRAVKLPNGNPVWQQPNGSGSVMPFRVDNAGQVYVEDVAHGWNDG
HHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCC
QKSWNNGNYDKWIANKGTTTMTCMNRQDLPWHYALADAFTICDQYFCSVMGPTDPNRYYL
CCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
WTGWDGNDGKNGGPVITNAEAGYDWTTFPERLEKAGISWKVYQDIGVGLTAAGYWGWTEN
EECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEEEEECCCCCC
PWIGNYGDNSLLYFHQYQNSQPGSPLYEKALRGSNIAKGGTFENLFDGLRNDVRNGTLPQ
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
VSWIAAPEAFSEHPNWLPGPGAWYISKVLDILTSNPELWSKTVLLINYDEGGGFFDHVVG
EEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHCC
PYPAMSQAWGQSTVDVSTDLFAGDANHVAGPYGLGVRVPMMVVSPWSRGGYVCSQVFDHT
CCCCHHHHCCCCEEECCCCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
SVIRFIEQRFHRHAKDLFETNIPAWRRAVCGDLTSAFNFTSPNEGMAPLPSTAGFKPPVA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
DITSGARFDDYHPVPPAKQVLPQQEPGIRPARALPYDLRVDGDADASNKKFGIRFVNPGQ
HHCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHCCCEEEEECCCCCCCCCEEEEEEECCCC
AGVCFHVRSGNTATGPWSYTVEAGKSLNGVWDLAASAGQYDLSVYGPNGFYRHFKGGVVS
CEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHCCCCEE
TAALIDVDTVHLCDREEGDDDRSLAIALTNKGAGCVVTIADNYTGRSERHRLQRGQRLET
EEEEEECCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCHHHH
QVRLRQTHGWYDLTVTTDTDANFNWQLAGHVENGRPSISDPAMGKIAAT
HHHHHHCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10523590 [H]