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The map label for this gene is emrA [H]

Identifier: 146342512

GI number: 146342512

Start: 5871288

End: 5872631

Strand: Reverse

Name: emrA [H]

Synonym: BRADO5674

Alternate gene names: 146342512

Gene position: 5872631-5871288 (Counterclockwise)

Preceding gene: 146342514

Following gene: 146342511

Centisome position: 78.76

GC content: 68.82

Gene sequence:

>1344_bases
ATGGCTGCTGCGAGAGAACAGGCTGTGCGGGCGCGTCGGCCGGATGCCGAGGAGGCATTGGGCCAGGCCAAGCACGATGC
CGTCCAGCGCAAGCAAGCCGAGACCAACCCGGTCGAGACCAAGACCGTTGATACCAAGACCGCCGCGCCAAAAGCGGACC
TCGGCGCCGAGACCCCGCTCGAGCTGCCGCCGGCTCCGCCCGCCTCAGCTCCCGCCGGCGCGAAGCCTCAGGCCGCGCCG
GCTGCTCCGCCGAAATCCGGCAAGCGCAAGCGCGTGCTCGCAGGCATAGGCCTTCTGCTCGCGCTCGCCGCAGCCGGCTA
CGCCACGCATTACATCATGGTCGGCCGCTTCATGGTCTCGACCGACGACTCCTATGTCCGCGCCAACAACACGATGCTCG
GCGCGCGCGTCTCGGGCCACATCCAGGAGATCGTGCCGCGCGACAACTCGGTCGTGCACAAGGGCGATGTGATCTTCCGC
ATCGACGACGGCGACTACAAGATCGCGGTCGACGCCGCGCGCACCAAGATCGCAACGCAGGAAGCAACGATCGCGCGCAT
CGGCCGCCAGGTCGCCGCGCAGCAGAGCGCGGTCGAGCAGGCGCAGGCCAATCTCACTTCCTCCGAGGCGGCGATGAAGC
GCGCCGGGCTCGACTATGAGCGCCAGCAGGCGCTGAGCAGCAAGGGCTTCGCTTCGCATGCGACCTTCGAGCAGTCGGAG
GCCGCGCGCGATCAGGGCCAGGCCGCGGTCCGCGCCGCGCAGGCGGCGTTCGACGCCGCGCGCGACAATGTCGAGGTCAC
CAAGGCGCAGCAGGCCGAGGCCCAGGCGCAACTCGCCGAACTGAAGACCTCGCTCGCCAAGGCCGAGCGCGATCTGGAGT
TCGCCATCGTCCGCGCGCCGGTCGACGGCACCTTCTCCAACCGCCTCGTGAGCACCGGCGACTTCGTCGCGGTTGGCCAG
CGGCTCGGCAACGTCGTGCCGCTCGATGATGTCTTCATCGATGCGAACTACAAGGAGACCCAGCTGAAGCGAATCCGTCC
CGGCCAGCCGGTGACGATCAAGGTCGACGCCTATGGCGCGCGCGGCTTCAAGGGCGTGGTCGACAGCATCTCGCCCGCCG
CCGGCTCGGTCTTCACTCTGCTGCCGCCCGACAATGCGACCGGCAACTTCACCAAGATCGTGCAGCGCCTGCCGGTTCGC
ATCCGCGTGCCGAAGGAGGTGGCGCGGCAGAACCTGCTGCGCGCCGGCATGTCCGTCTACGTGACGGTGGATACGACCGA
CGGCGCCAAGGATGCCGACAGCGACACCGACCTCGACGCGCCGGCGACGGTGCAGGGGAAGTAG

Upstream 100 bases:

>100_bases
GACCGAACGGTTCGGTCAATGATACAGATCAATTGACCGATCGGTTCGGTCAAATAATCCGGGGCCGGGGCGGCCCCGCT
TTGATGGGTGGGAGTCTCAG

Downstream 100 bases:

>100_bases
TTAGAACTGATGCGATCGTACTCGCGGTCGCGTCATCGGAAGTCAGCTCCCTCCCCCTTGTGGGGGAGGGCTGGGGAGGG
GGCCTCCGGGCGAGACCGCC

Product: putative multidrug resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MAAAREQAVRARRPDAEEALGQAKHDAVQRKQAETNPVETKTVDTKTAAPKADLGAETPLELPPAPPASAPAGAKPQAAP
AAPPKSGKRKRVLAGIGLLLALAAAGYATHYIMVGRFMVSTDDSYVRANNTMLGARVSGHIQEIVPRDNSVVHKGDVIFR
IDDGDYKIAVDAARTKIATQEATIARIGRQVAAQQSAVEQAQANLTSSEAAMKRAGLDYERQQALSSKGFASHATFEQSE
AARDQGQAAVRAAQAAFDAARDNVEVTKAQQAEAQAQLAELKTSLAKAERDLEFAIVRAPVDGTFSNRLVSTGDFVAVGQ
RLGNVVPLDDVFIDANYKETQLKRIRPGQPVTIKVDAYGARGFKGVVDSISPAAGSVFTLLPPDNATGNFTKIVQRLPVR
IRVPKEVARQNLLRAGMSVYVTVDTTDGAKDADSDTDLDAPATVQGK

Sequences:

>Translated_447_residues
MAAAREQAVRARRPDAEEALGQAKHDAVQRKQAETNPVETKTVDTKTAAPKADLGAETPLELPPAPPASAPAGAKPQAAP
AAPPKSGKRKRVLAGIGLLLALAAAGYATHYIMVGRFMVSTDDSYVRANNTMLGARVSGHIQEIVPRDNSVVHKGDVIFR
IDDGDYKIAVDAARTKIATQEATIARIGRQVAAQQSAVEQAQANLTSSEAAMKRAGLDYERQQALSSKGFASHATFEQSE
AARDQGQAAVRAAQAAFDAARDNVEVTKAQQAEAQAQLAELKTSLAKAERDLEFAIVRAPVDGTFSNRLVSTGDFVAVGQ
RLGNVVPLDDVFIDANYKETQLKRIRPGQPVTIKVDAYGARGFKGVVDSISPAAGSVFTLLPPDNATGNFTKIVQRLPVR
IRVPKEVARQNLLRAGMSVYVTVDTTDGAKDADSDTDLDAPATVQGK
>Mature_446_residues
AAAREQAVRARRPDAEEALGQAKHDAVQRKQAETNPVETKTVDTKTAAPKADLGAETPLELPPAPPASAPAGAKPQAAPA
APPKSGKRKRVLAGIGLLLALAAAGYATHYIMVGRFMVSTDDSYVRANNTMLGARVSGHIQEIVPRDNSVVHKGDVIFRI
DDGDYKIAVDAARTKIATQEATIARIGRQVAAQQSAVEQAQANLTSSEAAMKRAGLDYERQQALSSKGFASHATFEQSEA
ARDQGQAAVRAAQAAFDAARDNVEVTKAQQAEAQAQLAELKTSLAKAERDLEFAIVRAPVDGTFSNRLVSTGDFVAVGQR
LGNVVPLDDVFIDANYKETQLKRIRPGQPVTIKVDAYGARGFKGVVDSISPAAGSVFTLLPPDNATGNFTKIVQRLPVRI
RVPKEVARQNLLRAGMSVYVTVDTTDGAKDADSDTDLDAPATVQGK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=361, Percent_Identity=34.6260387811634, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1788711, Length=345, Percent_Identity=33.0434782608696, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1790519, Length=295, Percent_Identity=31.864406779661, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081951, Length=317, Percent_Identity=25.5520504731861, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789637, Length=307, Percent_Identity=26.7100977198697, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790012, Length=258, Percent_Identity=26.3565891472868, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790024, Length=267, Percent_Identity=25.4681647940075, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47392; Mature: 47261

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAREQAVRARRPDAEEALGQAKHDAVQRKQAETNPVETKTVDTKTAAPKADLGAETPL
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCC
ELPPAPPASAPAGAKPQAAPAAPPKSGKRKRVLAGIGLLLALAAAGYATHYIMVGRFMVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
TDDSYVRANNTMLGARVSGHIQEIVPRDNSVVHKGDVIFRIDDGDYKIAVDAARTKIATQ
CCCCEEEECCEEEECHHHHHHHHHCCCCCCEEECCCEEEEEECCCEEEEEEHHHHHHHHH
EATIARIGRQVAAQQSAVEQAQANLTSSEAAMKRAGLDYERQQALSSKGFASHATFEQSE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHH
AARDQGQAAVRAAQAAFDAARDNVEVTKAQQAEAQAQLAELKTSLAKAERDLEFAIVRAP
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
VDGTFSNRLVSTGDFVAVGQRLGNVVPLDDVFIDANYKETQLKRIRPGQPVTIKVDAYGA
CCCCCCCCEECCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCC
RGFKGVVDSISPAAGSVFTLLPPDNATGNFTKIVQRLPVRIRVPKEVARQNLLRAGMSVY
CCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHCCCEEE
VTVDTTDGAKDADSDTDLDAPATVQGK
EEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAAREQAVRARRPDAEEALGQAKHDAVQRKQAETNPVETKTVDTKTAAPKADLGAETPL
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCC
ELPPAPPASAPAGAKPQAAPAAPPKSGKRKRVLAGIGLLLALAAAGYATHYIMVGRFMVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
TDDSYVRANNTMLGARVSGHIQEIVPRDNSVVHKGDVIFRIDDGDYKIAVDAARTKIATQ
CCCCEEEECCEEEECHHHHHHHHHCCCCCCEEECCCEEEEEECCCEEEEEEHHHHHHHHH
EATIARIGRQVAAQQSAVEQAQANLTSSEAAMKRAGLDYERQQALSSKGFASHATFEQSE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHH
AARDQGQAAVRAAQAAFDAARDNVEVTKAQQAEAQAQLAELKTSLAKAERDLEFAIVRAP
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
VDGTFSNRLVSTGDFVAVGQRLGNVVPLDDVFIDANYKETQLKRIRPGQPVTIKVDAYGA
CCCCCCCCEECCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCC
RGFKGVVDSISPAAGSVFTLLPPDNATGNFTKIVQRLPVRIRVPKEVARQNLLRAGMSVY
CCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHCCCEEE
VTVDTTDGAKDADSDTDLDAPATVQGK
EEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]