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The map label for this gene is emrB [H]

Identifier: 146342511

GI number: 146342511

Start: 5869503

End: 5871092

Strand: Reverse

Name: emrB [H]

Synonym: BRADO5673

Alternate gene names: 146342511

Gene position: 5871092-5869503 (Counterclockwise)

Preceding gene: 146342512

Following gene: 146342510

Centisome position: 78.74

GC content: 64.59

Gene sequence:

>1590_bases
ATGGCCACCGCCACCACAGCCAACCCCACCATGAGCGCGCCCGCCGCGCCGGCGGATCGCATCGCCCCGCGCCGGCTGAT
CGCATTCCTGATCATGGTGTTCGGCATGTTCATGGCGATCCTGGACATCCAGATCGTCTCGGCGTCGCTGAGCGAGATCC
AGGCCGGCCTGTCCGCGAGCTCCAGCGAAGTCTCCTGGGTGCAGACCGCCTATCTCATCGCCGAGGTGATCGCGATCCCC
TTGTCCGGCTTCCTGTCGCGCGCGCTCGGCACGCGGCTGTTGTTCGCGATCTCGGCGGCCGGCTTCACCATCTCCAGCTT
CCTGTGCGGCTTCGCCTCGTCGATCGAGCAGATGATCGTCTGGCGCGCGCTGCAGGGCTTCCTCGGCGCCGGCATGATCC
CGACCGTGTTCGCCTCGGCCTACACGGTGTTTCCGCGTTCGAAGTTCCACATCGTTGGCCCGATCATCGGCCTTGTTGCG
ACGCTCGCGCCGACCGTCGGCCCGACGGTCGGCGGCTACATCACCGATGCGATGTCATGGCACTGGCTGTTCTTCGTCAA
CATCGTCCCCGGCGTCATCATCACCACCGGCGTGCTGGCGCTGGTCGATTTCGACCAGCCGAATTTCGCGCTGCTTGAAC
GGTTCGACTGGTGGGGCCTCTTGTTCATGGCGGGCTTCCTCGGCTCGCTGGAATATGTGCTGGAGGAGGGGCCGCAATAT
GAATGGCTGCAGGACACGTCCGTGGCGATCTGCGCCGCGGTCTGCGCCGTCTCGGCGGTGTGCTTCTTCTGGCGCGTGCT
GACTGCGGACGAGCCGATCGTCGACCTGTTCGCGTTCGGCAACCGCAACTTCGCGGTCGGCTGCCTCCTGCAGTTCTGCA
TCGGCATCGGCCTCTACGGCCTGACCTACATCTATCCGCGCTATCTCGCCGAGGTCCGCGGCTACAGCGCGCTGATGATC
GGCGAGACGATGTTCGTCTCGGGCGTGACGATGTTCCTGATGGCGCCGGTGGTCGGCCGCCTGATGCTGAAGATCGACAT
GCGGCTGATCATCGCCTTCGGCCTCGTGATCTTCGCGATCGGATCCTATGAGATGACCGGGATCACCCGCGACTACGATT
TCTGGGAGCTGTTCCTGCCGCAGGTGCTGCGCGGCGTCGGCATGATGTGCGCGATGGTTCCGACCAACAACATCGCGCTC
GGCACGCTCGCGCCCGATCGCGTCAAGAATGCCTCCGGCCTCTTCAACCTGATGCGCAATCTCGGCGGCGCCGTCGGCCT
CGCGGTCATCAACCAGGTGCTCAACGAGCGCACCGACCTGCATATCTCGCGCCTGCACGAGCGCGTGAACTGGGGCAATG
CGACCGCGGTCGAGACGCTGAACATGCTGCAGCAGAAGCTGCAGGGCATGGGCGACTCGGCCTTGATGGCGATGAAGCAG
CTGTCGCAGATCGTGCATCGCCAGGCGGTGGTGATGGGCTATGGCGATGCGTTCTTCATGCTGACATGCTTCTACGTCGC
GCTCAGCATGATGGTACTGCTGCTCAACAAGCCGAGCTCGCCGATGGCCGGCGGCGGCGACGCGCATTGA

Upstream 100 bases:

>100_bases
CCGCCGCTGTGGGCCCCCCTCCCCAGCCCCTCCCCACAAGGGGGAGGGGGGCGCACCACTCGTGCGGCGCGGTTATGGAC
CTCCAGCGGACTAACTGACC

Downstream 100 bases:

>100_bases
CGTGAGACGCCCCGCGGGGCCTGCGGGTTGCCAAATCATACCAGCGATGGTATGGAACGGGAATTGTCGCTCGAACCGGG
GAGATCTGCATGACTACATC

Product: putative multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MATATTANPTMSAPAAPADRIAPRRLIAFLIMVFGMFMAILDIQIVSASLSEIQAGLSASSSEVSWVQTAYLIAEVIAIP
LSGFLSRALGTRLLFAISAAGFTISSFLCGFASSIEQMIVWRALQGFLGAGMIPTVFASAYTVFPRSKFHIVGPIIGLVA
TLAPTVGPTVGGYITDAMSWHWLFFVNIVPGVIITTGVLALVDFDQPNFALLERFDWWGLLFMAGFLGSLEYVLEEGPQY
EWLQDTSVAICAAVCAVSAVCFFWRVLTADEPIVDLFAFGNRNFAVGCLLQFCIGIGLYGLTYIYPRYLAEVRGYSALMI
GETMFVSGVTMFLMAPVVGRLMLKIDMRLIIAFGLVIFAIGSYEMTGITRDYDFWELFLPQVLRGVGMMCAMVPTNNIAL
GTLAPDRVKNASGLFNLMRNLGGAVGLAVINQVLNERTDLHISRLHERVNWGNATAVETLNMLQQKLQGMGDSALMAMKQ
LSQIVHRQAVVMGYGDAFFMLTCFYVALSMMVLLLNKPSSPMAGGGDAH

Sequences:

>Translated_529_residues
MATATTANPTMSAPAAPADRIAPRRLIAFLIMVFGMFMAILDIQIVSASLSEIQAGLSASSSEVSWVQTAYLIAEVIAIP
LSGFLSRALGTRLLFAISAAGFTISSFLCGFASSIEQMIVWRALQGFLGAGMIPTVFASAYTVFPRSKFHIVGPIIGLVA
TLAPTVGPTVGGYITDAMSWHWLFFVNIVPGVIITTGVLALVDFDQPNFALLERFDWWGLLFMAGFLGSLEYVLEEGPQY
EWLQDTSVAICAAVCAVSAVCFFWRVLTADEPIVDLFAFGNRNFAVGCLLQFCIGIGLYGLTYIYPRYLAEVRGYSALMI
GETMFVSGVTMFLMAPVVGRLMLKIDMRLIIAFGLVIFAIGSYEMTGITRDYDFWELFLPQVLRGVGMMCAMVPTNNIAL
GTLAPDRVKNASGLFNLMRNLGGAVGLAVINQVLNERTDLHISRLHERVNWGNATAVETLNMLQQKLQGMGDSALMAMKQ
LSQIVHRQAVVMGYGDAFFMLTCFYVALSMMVLLLNKPSSPMAGGGDAH
>Mature_528_residues
ATATTANPTMSAPAAPADRIAPRRLIAFLIMVFGMFMAILDIQIVSASLSEIQAGLSASSSEVSWVQTAYLIAEVIAIPL
SGFLSRALGTRLLFAISAAGFTISSFLCGFASSIEQMIVWRALQGFLGAGMIPTVFASAYTVFPRSKFHIVGPIIGLVAT
LAPTVGPTVGGYITDAMSWHWLFFVNIVPGVIITTGVLALVDFDQPNFALLERFDWWGLLFMAGFLGSLEYVLEEGPQYE
WLQDTSVAICAAVCAVSAVCFFWRVLTADEPIVDLFAFGNRNFAVGCLLQFCIGIGLYGLTYIYPRYLAEVRGYSALMIG
ETMFVSGVTMFLMAPVVGRLMLKIDMRLIIAFGLVIFAIGSYEMTGITRDYDFWELFLPQVLRGVGMMCAMVPTNNIALG
TLAPDRVKNASGLFNLMRNLGGAVGLAVINQVLNERTDLHISRLHERVNWGNATAVETLNMLQQKLQGMGDSALMAMKQL
SQIVHRQAVVMGYGDAFFMLTCFYVALSMMVLLLNKPSSPMAGGGDAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=488, Percent_Identity=30.7377049180328, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1788710, Length=483, Percent_Identity=27.3291925465839, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87081983, Length=458, Percent_Identity=26.8558951965066, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=25, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788392, Length=423, Percent_Identity=26.9503546099291, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57439; Mature: 57307

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATATTANPTMSAPAAPADRIAPRRLIAFLIMVFGMFMAILDIQIVSASLSEIQAGLSAS
CCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSEVSWVQTAYLIAEVIAIPLSGFLSRALGTRLLFAISAAGFTISSFLCGFASSIEQMIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRALQGFLGAGMIPTVFASAYTVFPRSKFHIVGPIIGLVATLAPTVGPTVGGYITDAMSW
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HWLFFVNIVPGVIITTGVLALVDFDQPNFALLERFDWWGLLFMAGFLGSLEYVLEEGPQY
HHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EWLQDTSVAICAAVCAVSAVCFFWRVLTADEPIVDLFAFGNRNFAVGCLLQFCIGIGLYG
CHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LTYIYPRYLAEVRGYSALMIGETMFVSGVTMFLMAPVVGRLMLKIDMRLIIAFGLVIFAI
HHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSYEMTGITRDYDFWELFLPQVLRGVGMMCAMVPTNNIALGTLAPDRVKNASGLFNLMRN
CCEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHH
LGGAVGLAVINQVLNERTDLHISRLHERVNWGNATAVETLNMLQQKLQGMGDSALMAMKQ
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LSQIVHRQAVVMGYGDAFFMLTCFYVALSMMVLLLNKPSSPMAGGGDAH
HHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATATTANPTMSAPAAPADRIAPRRLIAFLIMVFGMFMAILDIQIVSASLSEIQAGLSAS
CCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSEVSWVQTAYLIAEVIAIPLSGFLSRALGTRLLFAISAAGFTISSFLCGFASSIEQMIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRALQGFLGAGMIPTVFASAYTVFPRSKFHIVGPIIGLVATLAPTVGPTVGGYITDAMSW
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HWLFFVNIVPGVIITTGVLALVDFDQPNFALLERFDWWGLLFMAGFLGSLEYVLEEGPQY
HHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EWLQDTSVAICAAVCAVSAVCFFWRVLTADEPIVDLFAFGNRNFAVGCLLQFCIGIGLYG
CHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LTYIYPRYLAEVRGYSALMIGETMFVSGVTMFLMAPVVGRLMLKIDMRLIIAFGLVIFAI
HHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSYEMTGITRDYDFWELFLPQVLRGVGMMCAMVPTNNIALGTLAPDRVKNASGLFNLMRN
CCEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHH
LGGAVGLAVINQVLNERTDLHISRLHERVNWGNATAVETLNMLQQKLQGMGDSALMAMKQ
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LSQIVHRQAVVMGYGDAFFMLTCFYVALSMMVLLLNKPSSPMAGGGDAH
HHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]