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The map label for this gene is mraW

Identifier: 146342506

GI number: 146342506

Start: 5862775

End: 5863770

Strand: Reverse

Name: mraW

Synonym: BRADO5667

Alternate gene names: 146342506

Gene position: 5863770-5862775 (Counterclockwise)

Preceding gene: 146342508

Following gene: 146342505

Centisome position: 78.64

GC content: 68.17

Gene sequence:

>996_bases
GTGAGCACGGCTGACCCACGGCACATCCCGGTGCTCGGGCCCGAGGCGGTTGCCATGCTGGCGCCGCGCGACGGCGGAAC
CTATGTGGATGCCACTTTCGGTGCGGGCGGTTACACCCGCCTGATCCTGGCGACACCGGGAACACGCGTCATCGCGATCG
ACCGCGACAGCACGGCGATCGCCGGCGGCGCCGATCTTGTCGGCGAGGCAAACGGTCGGCTGACGCTGGTGGAGAGCCGT
TTCTCTGCGCTGGCCGAGATCTGCGCCGCGCAAGCGGTGGGCCAGGTCGACGGCATCGTGATGGACGTCGGCGTGTCCTC
AATGCAGCTCGATACGGCGGCACGCGGGTTCTCGTTCCGGTTCGACGGTCCGCTTGATATGCGCATGGCTGACACCGGGC
CGTCCGCGGCCGATGTCATCGCGCAAGCCTCCGAGCGCGATCTGGCCGACGTCATCTACATTTTCGGTGAGGAGCGGCAT
TCCCGCGGCATCGCGCGCGCGATCGTAGCGGCGCGGAAGGAGCAGCAGATCACCACGACGCGACAGCTTGCCGACATCGT
CGCGCGCGTGGTGCGATCGAAGCCGGGCGACATTCATCCGGCAACGAGGACCTTTCAGGCGCTGCGCATTCTCGTGAATG
AGGAGCTCGACGAGCTGGAGCAGGCCCTGTCCGCCGCGGAGCGCATCCTCCCACCGAGCGGCCGTCTCGCCGTCGTTTCG
TTCCATTCACTCGAGGATCGAATCGTCAAGACGTTCCTGGCCGAGCGCAGCAAGATAGGTGGCGGCTCTCGCCATCTGCC
CGAGATCGCGCAGCAGGCGCCGAGCTTCCAGCTGCTCAATCGCAAGCCGGTGGTGGCCGACGAAGCCGAGACTGTGGCCA
ATCCGCGTGCGCGCTCCGCCAAGCTGCGCGGCGCCGCGCGCACGACAGCGCCGGCGCATCCGCCGTCCTCCTCGTCAGCC
TGGCCCCGCCTGGCCGATGTCATGCGGGGAGGGTGA

Upstream 100 bases:

>100_bases
ATGGCTTGGCAAGTTGACGGGGTGCGGGGATCGAAGGCGCGATGGACTCGCAGCCGAACAACATCAGTCCGGAACGGCGG
CCATCTCCGGGAGCGGCCCC

Downstream 100 bases:

>100_bases
CCGATGCGCCTCCTGCATCTCGTCGTGATCTGCTCGCTGATCTTCGCGGCGGCCTACGTCTATCGCATCAAGATGGAATC
GACCGCGCGTGTCGAGCATG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSTADPRHIPVLGPEAVAMLAPRDGGTYVDATFGAGGYTRLILATPGTRVIAIDRDSTAIAGGADLVGEANGRLTLVESR
FSALAEICAAQAVGQVDGIVMDVGVSSMQLDTAARGFSFRFDGPLDMRMADTGPSAADVIAQASERDLADVIYIFGEERH
SRGIARAIVAARKEQQITTTRQLADIVARVVRSKPGDIHPATRTFQALRILVNEELDELEQALSAAERILPPSGRLAVVS
FHSLEDRIVKTFLAERSKIGGGSRHLPEIAQQAPSFQLLNRKPVVADEAETVANPRARSAKLRGAARTTAPAHPPSSSSA
WPRLADVMRGG

Sequences:

>Translated_331_residues
MSTADPRHIPVLGPEAVAMLAPRDGGTYVDATFGAGGYTRLILATPGTRVIAIDRDSTAIAGGADLVGEANGRLTLVESR
FSALAEICAAQAVGQVDGIVMDVGVSSMQLDTAARGFSFRFDGPLDMRMADTGPSAADVIAQASERDLADVIYIFGEERH
SRGIARAIVAARKEQQITTTRQLADIVARVVRSKPGDIHPATRTFQALRILVNEELDELEQALSAAERILPPSGRLAVVS
FHSLEDRIVKTFLAERSKIGGGSRHLPEIAQQAPSFQLLNRKPVVADEAETVANPRARSAKLRGAARTTAPAHPPSSSSA
WPRLADVMRGG
>Mature_330_residues
STADPRHIPVLGPEAVAMLAPRDGGTYVDATFGAGGYTRLILATPGTRVIAIDRDSTAIAGGADLVGEANGRLTLVESRF
SALAEICAAQAVGQVDGIVMDVGVSSMQLDTAARGFSFRFDGPLDMRMADTGPSAADVIAQASERDLADVIYIFGEERHS
RGIARAIVAARKEQQITTTRQLADIVARVVRSKPGDIHPATRTFQALRILVNEELDELEQALSAAERILPPSGRLAVVSF
HSLEDRIVKTFLAERSKIGGGSRHLPEIAQQAPSFQLLNRKPVVADEAETVANPRARSAKLRGAARTTAPAHPPSSSSAW
PRLADVMRGG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=41.6666666666667, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=44.973544973545, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=48.2315112540193, Blast_Score=249, Evalue=2e-67,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=40.9638554216867, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=40.9638554216867, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BRASO (A4YZL1)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001207554.1
- ProteinModelPortal:   A4YZL1
- STRING:   A4YZL1
- GeneID:   5115432
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO5667
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   BSP376:BRADO5667-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35130; Mature: 34999

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTADPRHIPVLGPEAVAMLAPRDGGTYVDATFGAGGYTRLILATPGTRVIAIDRDSTAI
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCCEE
AGGADLVGEANGRLTLVESRFSALAEICAAQAVGQVDGIVMDVGVSSMQLDTAARGFSFR
CCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEE
FDGPLDMRMADTGPSAADVIAQASERDLADVIYIFGEERHSRGIARAIVAARKEQQITTT
ECCCCCEEEECCCCCHHHHHHHHCCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
RQLADIVARVVRSKPGDIHPATRTFQALRILVNEELDELEQALSAAERILPPSGRLAVVS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FHSLEDRIVKTFLAERSKIGGGSRHLPEIAQQAPSFQLLNRKPVVADEAETVANPRARSA
EHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCHHHH
KLRGAARTTAPAHPPSSSSAWPRLADVMRGG
HHCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
STADPRHIPVLGPEAVAMLAPRDGGTYVDATFGAGGYTRLILATPGTRVIAIDRDSTAI
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCCEE
AGGADLVGEANGRLTLVESRFSALAEICAAQAVGQVDGIVMDVGVSSMQLDTAARGFSFR
CCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEE
FDGPLDMRMADTGPSAADVIAQASERDLADVIYIFGEERHSRGIARAIVAARKEQQITTT
ECCCCCEEEECCCCCHHHHHHHHCCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
RQLADIVARVVRSKPGDIHPATRTFQALRILVNEELDELEQALSAAERILPPSGRLAVVS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FHSLEDRIVKTFLAERSKIGGGSRHLPEIAQQAPSFQLLNRKPVVADEAETVANPRARSA
EHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCHHHH
KLRGAARTTAPAHPPSSSSAWPRLADVMRGG
HHCCCCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA