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The map label for this gene is 146342220

Identifier: 146342220

GI number: 146342220

Start: 5578309

End: 5579232

Strand: Reverse

Name: 146342220

Synonym: BRADO5372

Alternate gene names: NA

Gene position: 5579232-5578309 (Counterclockwise)

Preceding gene: 146342221

Following gene: 146342219

Centisome position: 74.82

GC content: 62.77

Gene sequence:

>924_bases
ATGTCAAAGCGCGTCAGCAAGGCGGGCAACATCAAATACGCGCTCTCCGGCGATGATAAGTTCATCGCGTCAGTGGAGCC
CGGTGAGATCTTCGTCGTCGAATGCGCGATCAACGTCAATGACGGCACCATTCGGGCACTCGGCCAGCAACTGGTCGAAA
GCGACGTGACGCTGCCTTACGTCAACGGTGCGACCGGTCCGATCGAGGTGCGCGGTGCCAAGGCCGGCGACATGCTGCGG
CTCGACATCATCGACATGGAGCTCGACCGCCTCGGATTCACGGCACTATGGCCCGGCATCGGCATGTTCCCCGACTGGGT
TCGGCGCAAGGAGTTCGGCATCCAGACCCGTGTCGTCGCCGTCGAGAACGGTCAGGTGCTGTGGAACGAGCATGTGAAGC
TGCCGGTGCGGCCGATGATTGGCGTCGCTGGCGTGGCGCCGGTCCATGGAGCGGTGCTGACGGTCGACAACGGCCCGCAT
GGCGGCAATCTCGATGTGCAGGAGATCACGTCGGGCAATTCGGTGTTCTTCCGCGTCAACAAGGATGGCGCGCATCTTTT
CCTCGGCGACTGCCACGCCATCCAGGGCGATGGCGAGGCCAACGGCATGGGCGCGATCGAGATCGCGGCGAACCTCACCG
TCAAGGTCTCGCTCGACAAGGCACCGGCGCGGCTGAACCATCCGCGTATCGAGACACCGACGCATATCTGCACGCTCGGC
TGTGCGCGCCCCCTGGAGGATGCGATGCGGATCGCGTTCGAGGAGATGATCAACTGGCTCGAGGATGATTGGAAGATCCC
GGCAGCGGAGGCCTATATGCTGCTCGGCCAGGTCGCCGAGGCGCGCTGTACCCAGGTGGTCAATCCGAAATACACCTACA
TCTGCAAGGTCAACAAGACCTTGCTCGCCGGCTATCACGGCTGA

Upstream 100 bases:

>100_bases
CAGCTTCTGATGCCATGGCGACGGCAGCTGCAACGATGAGGCGGGATTCTCCGATCATGCATCACCAGAAACGAAACGGC
GGACAAAACAGGGAGTGATC

Downstream 100 bases:

>100_bases
GGCCGTTCACTCGAGACGAAGGAGGACATCATGGATCTGGGATTGGCGGGCAAGAGCGTGCTGATCACGGGCGGTAGCAA
GGGTATCGGACTCGCGATCG

Product: putative acetamidase/formamidase

Products: formate; NH3

Alternate protein names: Acetamidase/Formamidase Family Protein; Formamidase; Acetamidase; Amidase; Acetamidase/Formamidase Protein; Amidase-Related Protein; Acetamidase/Formamidase Family; Amidae; Transcriptional Regulatory Protein

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSKRVSKAGNIKYALSGDDKFIASVEPGEIFVVECAINVNDGTIRALGQQLVESDVTLPYVNGATGPIEVRGAKAGDMLR
LDIIDMELDRLGFTALWPGIGMFPDWVRRKEFGIQTRVVAVENGQVLWNEHVKLPVRPMIGVAGVAPVHGAVLTVDNGPH
GGNLDVQEITSGNSVFFRVNKDGAHLFLGDCHAIQGDGEANGMGAIEIAANLTVKVSLDKAPARLNHPRIETPTHICTLG
CARPLEDAMRIAFEEMINWLEDDWKIPAAEAYMLLGQVAEARCTQVVNPKYTYICKVNKTLLAGYHG

Sequences:

>Translated_307_residues
MSKRVSKAGNIKYALSGDDKFIASVEPGEIFVVECAINVNDGTIRALGQQLVESDVTLPYVNGATGPIEVRGAKAGDMLR
LDIIDMELDRLGFTALWPGIGMFPDWVRRKEFGIQTRVVAVENGQVLWNEHVKLPVRPMIGVAGVAPVHGAVLTVDNGPH
GGNLDVQEITSGNSVFFRVNKDGAHLFLGDCHAIQGDGEANGMGAIEIAANLTVKVSLDKAPARLNHPRIETPTHICTLG
CARPLEDAMRIAFEEMINWLEDDWKIPAAEAYMLLGQVAEARCTQVVNPKYTYICKVNKTLLAGYHG
>Mature_306_residues
SKRVSKAGNIKYALSGDDKFIASVEPGEIFVVECAINVNDGTIRALGQQLVESDVTLPYVNGATGPIEVRGAKAGDMLRL
DIIDMELDRLGFTALWPGIGMFPDWVRRKEFGIQTRVVAVENGQVLWNEHVKLPVRPMIGVAGVAPVHGAVLTVDNGPHG
GNLDVQEITSGNSVFFRVNKDGAHLFLGDCHAIQGDGEANGMGAIEIAANLTVKVSLDKAPARLNHPRIETPTHICTLGC
ARPLEDAMRIAFEEMINWLEDDWKIPAAEAYMLLGQVAEARCTQVVNPKYTYICKVNKTLLAGYHG

Specific function: Unknown

COG id: COG2421

COG function: function code C; Predicted acetamidase/formamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.49

Molecular weight: Translated: 33312; Mature: 33181

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVSKAGNIKYALSGDDKFIASVEPGEIFVVECAINVNDGTIRALGQQLVESDVTLPY
CCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEE
VNGATGPIEVRGAKAGDMLRLDIIDMELDRLGFTALWPGIGMFPDWVRRKEFGIQTRVVA
ECCCCCCEEEECCCCCCEEEEEEEEEEHHHCCCEEECCCCCCCHHHHHHHHCCCEEEEEE
VENGQVLWNEHVKLPVRPMIGVAGVAPVHGAVLTVDNGPHGGNLDVQEITSGNSVFFRVN
EECCEEEEECCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCEEHHEECCCCEEEEEEC
KDGAHLFLGDCHAIQGDGEANGMGAIEIAANLTVKVSLDKAPARLNHPRIETPTHICTLG
CCCCEEEEEEEEEECCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEC
CARPLEDAMRIAFEEMINWLEDDWKIPAAEAYMLLGQVAEARCTQVVNPKYTYICKVNKT
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCE
LLAGYHG
EEEECCC
>Mature Secondary Structure 
SKRVSKAGNIKYALSGDDKFIASVEPGEIFVVECAINVNDGTIRALGQQLVESDVTLPY
CCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEE
VNGATGPIEVRGAKAGDMLRLDIIDMELDRLGFTALWPGIGMFPDWVRRKEFGIQTRVVA
ECCCCCCEEEECCCCCCEEEEEEEEEEHHHCCCEEECCCCCCCHHHHHHHHCCCEEEEEE
VENGQVLWNEHVKLPVRPMIGVAGVAPVHGAVLTVDNGPHGGNLDVQEITSGNSVFFRVN
EECCEEEEECCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCEEHHEECCCCEEEEEEC
KDGAHLFLGDCHAIQGDGEANGMGAIEIAANLTVKVSLDKAPARLNHPRIETPTHICTLG
CCCCEEEEEEEEEECCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEC
CARPLEDAMRIAFEEMINWLEDDWKIPAAEAYMLLGQVAEARCTQVVNPKYTYICKVNKT
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCE
LLAGYHG
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formamide; H2O

Specific reaction: formamide + H2O = formate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA