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The map label for this gene is 146342159

Identifier: 146342159

GI number: 146342159

Start: 5523070

End: 5524620

Strand: Direct

Name: 146342159

Synonym: BRADO5309

Alternate gene names: NA

Gene position: 5523070-5524620 (Clockwise)

Preceding gene: 146342158

Following gene: 146342162

Centisome position: 74.07

GC content: 59.9

Gene sequence:

>1551_bases
ATGACCTCATCGTCCGATCAGGATCTCGTCTTCGCCTTCCTGGGCGACACCGATCGCCATGCGGACGTCCGCAGGATCGA
CACCCACGCAGCTTCGGTTTTCCTGTTCGGCAAACGCGCCTTCAAGATCAAGCGGGCGATCCGGCTGCCGTTTCTCGACT
ATTCGACGCTCGCGCTCCGGCACGCCGCCTGCACGAAGGAGTTGGAGATCAACCGCCCGTTCGCACCCGACATCTACCTG
CGCGTGGTCCCGATCACCCGTGCGGCGGACGGATCGCTCGATATCGATGGATCCGGTGCCCCTATCGAATATGCCGTCGA
GATGCGTCGCTTCGACGATAACAATACGCTCGATCACCTCGCACGCCGAGGCGAGCTGACCGCTAGATTGGCCGACGATC
TGGCTGAGACGGTCGCAGCCTCGCATCGAGTCGCAGCAGAAGTCCCGCCTGACTCGTGGATCAGGAGTATCCCGCGTTGG
ATCGACGCTTTCCTTGAGAGCTTCGAATCCAACCCGCGGATGGCGCGGATCGAAATCGAGGAGCTCCGCGAACTCTGCCA
TGCGGCGCTGTCCCGGCTCACGCCAATTCTGGAGCATCGCGTCGAAAAGGGTTATGTCCGGCGCTGTCATGGTGACCTTC
ATCTGGCAAATATCGTGGTGATCGACCAAAGACCGGTGCTGTTCGACGCCATCGAGTTCGATGATAGCATCGCTACGATC
GACATTCTCTACGACCTCGCGTTTCCGCTGATGGATATGCTGTACTTCGATCTGCCGGTTGCGGCCAATCATCTCCTGAA
TCGTTATCTGTCGATAGCCGCCTCGGACCAGATCGATGGCTTGATACTGTTACCTCTGTTCATGACGATTCGCGCCGCCA
TCCGCGCACAGGTCTTCCTGGCAAAGCTCGATCGTGGCGACACCGATCATACCGATCCCGACGGCTCCGCAACGTTCGAG
ACTGCCACGCGTTATTTCCAGCTTGCGCGGCAGCTTATTCGGCCATCGGCTCCGATGCTGATTGCCGTCGGCGGGCTCTC
AGGAACCGGCAAATCGGCCCTGGCAAGAGCGCTGGCACCTATCCTGAAGCCGCGACCGGGAGCGATCGTTGTCCGCAGCG
ACGTGCTGCGCAAACATTTATTTGGCGTCGCCGAAACCGAGCGACTGCCCCAAAAGGCCTATTCGCCCGACGCGAGCTTA
CGAGTCTATCATGAACTGACGAAAACAGCAGCTCGTGTGCTGGCGCAGGGTTTCGCGGTCGTGGTCGACGCTGTGTTTGC
AAAACACGATGAGCGAGAGGAGATCCGGACACTGGCGACCCGGCAGAACGTGCCCTTCGCCGGCCTCTTTCTCGTTGCCG
ACCTCCGCATCCGACAAGAGCGGATAGAGGGACGAGTCAACGACGCTTCCGATGCAACCAGCGCGATTGCGCAGGATCAG
GAACGCTATGACATCGGAATGCTGGATTGGCTCCGCGTGGACGCGTCCGGAACGCCAGAATCCACGCTGGACAAAAGCCT
GTCAGTGTTGCGGATCGGCAGGTCAGACTGA

Upstream 100 bases:

>100_bases
ACCGATGCGAATGATGTTCTGAACCGCTTGAGGCACGGCGACGTTCTCGGGGCCGCCGTTCTCGTTCCGGACTTCGGATT
CTGATGCAAGAGCTGCGGCC

Downstream 100 bases:

>100_bases
GGCTTCTGGACTCATGGCCAAACCCGTGGCGAACTGTGCCGAAGGACGACCTCACGTGTGCACATACTCGCCTGGCGCGT
CCCCAAGCTCATGTCCGTCA

Product: nucleotide-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTSSSDQDLVFAFLGDTDRHADVRRIDTHAASVFLFGKRAFKIKRAIRLPFLDYSTLALRHAACTKELEINRPFAPDIYL
RVVPITRAADGSLDIDGSGAPIEYAVEMRRFDDNNTLDHLARRGELTARLADDLAETVAASHRVAAEVPPDSWIRSIPRW
IDAFLESFESNPRMARIEIEELRELCHAALSRLTPILEHRVEKGYVRRCHGDLHLANIVVIDQRPVLFDAIEFDDSIATI
DILYDLAFPLMDMLYFDLPVAANHLLNRYLSIAASDQIDGLILLPLFMTIRAAIRAQVFLAKLDRGDTDHTDPDGSATFE
TATRYFQLARQLIRPSAPMLIAVGGLSGTGKSALARALAPILKPRPGAIVVRSDVLRKHLFGVAETERLPQKAYSPDASL
RVYHELTKTAARVLAQGFAVVVDAVFAKHDEREEIRTLATRQNVPFAGLFLVADLRIRQERIEGRVNDASDATSAIAQDQ
ERYDIGMLDWLRVDASGTPESTLDKSLSVLRIGRSD

Sequences:

>Translated_516_residues
MTSSSDQDLVFAFLGDTDRHADVRRIDTHAASVFLFGKRAFKIKRAIRLPFLDYSTLALRHAACTKELEINRPFAPDIYL
RVVPITRAADGSLDIDGSGAPIEYAVEMRRFDDNNTLDHLARRGELTARLADDLAETVAASHRVAAEVPPDSWIRSIPRW
IDAFLESFESNPRMARIEIEELRELCHAALSRLTPILEHRVEKGYVRRCHGDLHLANIVVIDQRPVLFDAIEFDDSIATI
DILYDLAFPLMDMLYFDLPVAANHLLNRYLSIAASDQIDGLILLPLFMTIRAAIRAQVFLAKLDRGDTDHTDPDGSATFE
TATRYFQLARQLIRPSAPMLIAVGGLSGTGKSALARALAPILKPRPGAIVVRSDVLRKHLFGVAETERLPQKAYSPDASL
RVYHELTKTAARVLAQGFAVVVDAVFAKHDEREEIRTLATRQNVPFAGLFLVADLRIRQERIEGRVNDASDATSAIAQDQ
ERYDIGMLDWLRVDASGTPESTLDKSLSVLRIGRSD
>Mature_515_residues
TSSSDQDLVFAFLGDTDRHADVRRIDTHAASVFLFGKRAFKIKRAIRLPFLDYSTLALRHAACTKELEINRPFAPDIYLR
VVPITRAADGSLDIDGSGAPIEYAVEMRRFDDNNTLDHLARRGELTARLADDLAETVAASHRVAAEVPPDSWIRSIPRWI
DAFLESFESNPRMARIEIEELRELCHAALSRLTPILEHRVEKGYVRRCHGDLHLANIVVIDQRPVLFDAIEFDDSIATID
ILYDLAFPLMDMLYFDLPVAANHLLNRYLSIAASDQIDGLILLPLFMTIRAAIRAQVFLAKLDRGDTDHTDPDGSATFET
ATRYFQLARQLIRPSAPMLIAVGGLSGTGKSALARALAPILKPRPGAIVVRSDVLRKHLFGVAETERLPQKAYSPDASLR
VYHELTKTAARVLAQGFAVVVDAVFAKHDEREEIRTLATRQNVPFAGLFLVADLRIRQERIEGRVNDASDATSAIAQDQE
RYDIGMLDWLRVDASGTPESTLDKSLSVLRIGRSD

Specific function: Some isolated peptides of this protein are able to bind to human U937 monocytoblastic and A549 epithelial cell lines [H]

COG id: COG2187

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR010488 [H]

Pfam domain/function: PF06414 Zeta_toxin [H]

EC number: NA

Molecular weight: Translated: 57554; Mature: 57423

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSSDQDLVFAFLGDTDRHADVRRIDTHAASVFLFGKRAFKIKRAIRLPFLDYSTLALR
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHEEEECHHHHHHHHHHHCCCCCHHHHHHH
HAACTKELEINRPFAPDIYLRVVPITRAADGSLDIDGSGAPIEYAVEMRRFDDNNTLDHL
HHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHH
ARRGELTARLADDLAETVAASHRVAAEVPPDSWIRSIPRWIDAFLESFESNPRMARIEIE
HHHCCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
ELRELCHAALSRLTPILEHRVEKGYVRRCHGDLHLANIVVIDQRPVLFDAIEFDDSIATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHH
DILYDLAFPLMDMLYFDLPVAANHLLNRYLSIAASDQIDGLILLPLFMTIRAAIRAQVFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
AKLDRGDTDHTDPDGSATFETATRYFQLARQLIRPSAPMLIAVGGLSGTGKSALARALAP
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
ILKPRPGAIVVRSDVLRKHLFGVAETERLPQKAYSPDASLRVYHELTKTAARVLAQGFAV
HHCCCCCEEEEHHHHHHHHHHCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VVDAVFAKHDEREEIRTLATRQNVPFAGLFLVADLRIRQERIEGRVNDASDATSAIAQDQ
HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCH
ERYDIGMLDWLRVDASGTPESTLDKSLSVLRIGRSD
HHCCCCCCCEEEECCCCCCHHHHHHCHHEEECCCCC
>Mature Secondary Structure 
TSSSDQDLVFAFLGDTDRHADVRRIDTHAASVFLFGKRAFKIKRAIRLPFLDYSTLALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHEEEECHHHHHHHHHHHCCCCCHHHHHHH
HAACTKELEINRPFAPDIYLRVVPITRAADGSLDIDGSGAPIEYAVEMRRFDDNNTLDHL
HHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHH
ARRGELTARLADDLAETVAASHRVAAEVPPDSWIRSIPRWIDAFLESFESNPRMARIEIE
HHHCCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
ELRELCHAALSRLTPILEHRVEKGYVRRCHGDLHLANIVVIDQRPVLFDAIEFDDSIATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHH
DILYDLAFPLMDMLYFDLPVAANHLLNRYLSIAASDQIDGLILLPLFMTIRAAIRAQVFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
AKLDRGDTDHTDPDGSATFETATRYFQLARQLIRPSAPMLIAVGGLSGTGKSALARALAP
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
ILKPRPGAIVVRSDVLRKHLFGVAETERLPQKAYSPDASLRVYHELTKTAARVLAQGFAV
HHCCCCCEEEEHHHHHHHHHHCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VVDAVFAKHDEREEIRTLATRQNVPFAGLFLVADLRIRQERIEGRVNDASDATSAIAQDQ
HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCH
ERYDIGMLDWLRVDASGTPESTLDKSLSVLRIGRSD
HHCCCCCCCEEEECCCCCCHHHHHHCHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]