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The map label for this gene is adhA [H]

Identifier: 146342158

GI number: 146342158

Start: 5522058

End: 5523053

Strand: Direct

Name: adhA [H]

Synonym: BRADO5308

Alternate gene names: 146342158

Gene position: 5522058-5523053 (Clockwise)

Preceding gene: 146342156

Following gene: 146342159

Centisome position: 74.06

GC content: 63.45

Gene sequence:

>996_bases
ATGCACGCGATGGTGTTGCAAAAACCGGGGGGACCTCTTCGACTTGAGACGCTTGCCGATCCTGAGCCGCGTCCTGGTGA
GATTCGGGTCCGGGTCAGCGCGTGCGGTGTCTGCCGCACCGATCTGCATCTTCTCGATGGCGAACTGCCCGACATCGTCT
ATCCGATCATTCCGGGTCACGAGATCGTCGGCCACATCGACCGTATCGGAGCAGGCGTCACGACGCATCGGCTTGGGACA
CGGGTCGGAATTCCCTGGCTGGGCCACACCTGTGGACTCTGCCCCTTCTGCGCGCGTGGAATGGAGAATCTCTGCGACGC
GCCCGGATTGACCGGCTACACGCGCCATGGCGGATACGCCACGCACGCCGTTGCCGATGCGAGTTATGCGTTCCCGCTAG
AAGGTCTGGGCGACGACGTCGCCGCTGCACCCTTGCTGTGCGCCGGCCTGATCGGCTGGCGATCGCTGGTCATGGCCGGC
GACGCAGAGCACTTGGGCATCTTCGGTTTCGGAGCCGCAGGTCATATCGTCGTCCAAGTCGCCCGCTGGCAGGGGCGCAA
GGTCTATGCGTTCACCCGACGAGACGATCGGACCGCGCAGGATCTCGCGCTCCAACTCGGTGCGACATGGGCGGGTCCGT
CCGACGCGCGTCCTGATGTTCCACTGGACGCGGCCATTATTTATGCGCCTGCCGGTGAGCTTGTCCCCGCCGCATTGAAA
GCGGTGCGGAAGGGTGGTCGGGTCGTGTGCGCGGGCATCCACATGAGCGAGATTCCGAGCTTCCCCTACTCGCTTCTCTG
GGGCGAACGTCACGTGATGTCTGTTGCCAACCTGACTCGACAGGATGGTCTGGATTTCCTGCGTGTCGCTCGTGAGGCGC
AGATCCGCACTCACACGACGCCGTATCCACTGACCGATGCGAATGATGTTCTGAACCGCTTGAGGCACGGCGACGTTCTC
GGGGCCGCCGTTCTCGTTCCGGACTTCGGATTCTGA

Upstream 100 bases:

>100_bases
CTGATAGTCTGCGGACGTCGCTGAGCACCTTGAGGAACATCAAACCGGTGCGCCCCGGGTTGGCTTATGCGATCGGCCAC
AAGTCTCCAGGAGAGCCTCG

Downstream 100 bases:

>100_bases
TGCAAGAGCTGCGGCCATGACCTCATCGTCCGATCAGGATCTCGTCTTCGCCTTCCTGGGCGACACCGATCGCCATGCGG
ACGTCCGCAGGATCGACACC

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MHAMVLQKPGGPLRLETLADPEPRPGEIRVRVSACGVCRTDLHLLDGELPDIVYPIIPGHEIVGHIDRIGAGVTTHRLGT
RVGIPWLGHTCGLCPFCARGMENLCDAPGLTGYTRHGGYATHAVADASYAFPLEGLGDDVAAAPLLCAGLIGWRSLVMAG
DAEHLGIFGFGAAGHIVVQVARWQGRKVYAFTRRDDRTAQDLALQLGATWAGPSDARPDVPLDAAIIYAPAGELVPAALK
AVRKGGRVVCAGIHMSEIPSFPYSLLWGERHVMSVANLTRQDGLDFLRVAREAQIRTHTTPYPLTDANDVLNRLRHGDVL
GAAVLVPDFGF

Sequences:

>Translated_331_residues
MHAMVLQKPGGPLRLETLADPEPRPGEIRVRVSACGVCRTDLHLLDGELPDIVYPIIPGHEIVGHIDRIGAGVTTHRLGT
RVGIPWLGHTCGLCPFCARGMENLCDAPGLTGYTRHGGYATHAVADASYAFPLEGLGDDVAAAPLLCAGLIGWRSLVMAG
DAEHLGIFGFGAAGHIVVQVARWQGRKVYAFTRRDDRTAQDLALQLGATWAGPSDARPDVPLDAAIIYAPAGELVPAALK
AVRKGGRVVCAGIHMSEIPSFPYSLLWGERHVMSVANLTRQDGLDFLRVAREAQIRTHTTPYPLTDANDVLNRLRHGDVL
GAAVLVPDFGF
>Mature_331_residues
MHAMVLQKPGGPLRLETLADPEPRPGEIRVRVSACGVCRTDLHLLDGELPDIVYPIIPGHEIVGHIDRIGAGVTTHRLGT
RVGIPWLGHTCGLCPFCARGMENLCDAPGLTGYTRHGGYATHAVADASYAFPLEGLGDDVAAAPLLCAGLIGWRSLVMAG
DAEHLGIFGFGAAGHIVVQVARWQGRKVYAFTRRDDRTAQDLALQLGATWAGPSDARPDVPLDAAIIYAPAGELVPAALK
AVRKGGRVVCAGIHMSEIPSFPYSLLWGERHVMSVANLTRQDGLDFLRVAREAQIRTHTTPYPLTDANDVLNRLRHGDVL
GAAVLVPDFGF

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: COG1064

COG function: function code R; Zn-dependent alcohol dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501939, Length=134, Percent_Identity=31.3432835820896, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI156523966, Length=134, Percent_Identity=31.3432835820896, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI71565154, Length=110, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4501933, Length=248, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI262073058, Length=240, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI71743840, Length=240, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI71565152, Length=104, Percent_Identity=33.6538461538462, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI34577061, Length=242, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4501929, Length=248, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87081918, Length=323, Percent_Identity=30.030959752322, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87082401, Length=338, Percent_Identity=26.6272189349112, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786518, Length=209, Percent_Identity=33.9712918660287, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787863, Length=224, Percent_Identity=30.8035714285714, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI226510992, Length=340, Percent_Identity=25, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786552, Length=307, Percent_Identity=30.2931596091205, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788407, Length=210, Percent_Identity=29.0476190476191, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788073, Length=328, Percent_Identity=24.0853658536585, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562584, Length=345, Percent_Identity=28.1159420289855, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17562582, Length=344, Percent_Identity=26.453488372093, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=337, Percent_Identity=25.8160237388724, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25146526, Length=104, Percent_Identity=34.6153846153846, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=268, Percent_Identity=28.7313432835821, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=342, Percent_Identity=26.9005847953216, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319621, Length=340, Percent_Identity=26.7647058823529, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323961, Length=190, Percent_Identity=33.1578947368421, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324486, Length=191, Percent_Identity=32.9842931937173, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323980, Length=332, Percent_Identity=26.5060240963855, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320033, Length=362, Percent_Identity=25.414364640884, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319949, Length=328, Percent_Identity=25.609756097561, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17737895, Length=110, Percent_Identity=35.4545454545455, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR014187
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1 [H]

Molecular weight: Translated: 35398; Mature: 35398

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAMVLQKPGGPLRLETLADPEPRPGEIRVRVSACGVCRTDLHLLDGELPDIVYPIIPGH
CCCEEEECCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCEEEECCCCH
EIVGHIDRIGAGVTTHRLGTRVGIPWLGHTCGLCPFCARGMENLCDAPGLTGYTRHGGYA
HHHHHHHHHCCCCCHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
THAVADASYAFPLEGLGDDVAAAPLLCAGLIGWRSLVMAGDAEHLGIFGFGAAGHIVVQV
EHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEE
ARWQGRKVYAFTRRDDRTAQDLALQLGATWAGPSDARPDVPLDAAIIYAPAGELVPAALK
EECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHH
AVRKGGRVVCAGIHMSEIPSFPYSLLWGERHVMSVANLTRQDGLDFLRVAREAQIRTHTT
HHHCCCCEEEEECCHHHCCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHEECCC
PYPLTDANDVLNRLRHGDVLGAAVLVPDFGF
CCCCCCHHHHHHHHHCCCHHEEEEECCCCCC
>Mature Secondary Structure
MHAMVLQKPGGPLRLETLADPEPRPGEIRVRVSACGVCRTDLHLLDGELPDIVYPIIPGH
CCCEEEECCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCEEEECCCCH
EIVGHIDRIGAGVTTHRLGTRVGIPWLGHTCGLCPFCARGMENLCDAPGLTGYTRHGGYA
HHHHHHHHHCCCCCHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
THAVADASYAFPLEGLGDDVAAAPLLCAGLIGWRSLVMAGDAEHLGIFGFGAAGHIVVQV
EHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEE
ARWQGRKVYAFTRRDDRTAQDLALQLGATWAGPSDARPDVPLDAAIIYAPAGELVPAALK
EECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHH
AVRKGGRVVCAGIHMSEIPSFPYSLLWGERHVMSVANLTRQDGLDFLRVAREAQIRTHTT
HHHCCCCEEEEECCHHHCCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHEECCC
PYPLTDANDVLNRLRHGDVLGAAVLVPDFGF
CCCCCCHHHHHHHHHCCCHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]