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The map label for this gene is braE [H]

Identifier: 146342108

GI number: 146342108

Start: 5463746

End: 5464783

Strand: Reverse

Name: braE [H]

Synonym: BRADO5252

Alternate gene names: 146342108

Gene position: 5464783-5463746 (Counterclockwise)

Preceding gene: 146342109

Following gene: 146342107

Centisome position: 73.29

GC content: 64.84

Gene sequence:

>1038_bases
ATGACAACGAATGCACAAACCGAGGTCGGCTTCCACGCCCGGCAGCATGGCCGCTGGCACTGGAGCGAGTTCGCCTTCTG
GATCGTCGTGCTGGCGGTGGGGCTCGCCGTTCCGTCGCGCTATTTGATCATGACCGAGATCCTGCGGCTGGCGCTGTTCG
CGCTGTCGCTCGATTTGATCCTCGGCTATGCCGGCATCGTTTCGCTCGGCCACGCCGCCTTCTTCGGCGTCGGCGCCTAT
TGCGCCGGGCTGCTGTCGGTGCACGGGATCATCAACGAGCCGGTGCTGGCGCTGATCGTGGCCGGCCTCGCCGCCACGGT
GCTCGGCTTCGCCACCAGCTTCCTCGTCATCCGCGGCGTCGACCTGACGCGGCTGATGGTTACGCTGGGCATCGCACTGC
TGATGGAAGCGCTCGCCGAGCGCTTCTCCAACATCACCGGCGGTACCGACGGCCTGCAGGGCATCGACATCCAGCCGATC
TTCGGCCTGTTCGCCTTCGACATGTTCGGCAGGACTGGCTTCTTCTATTCGCTCGCAGTGCTGTTCGTGCTGTTCCTGCT
GGCGCGGATGATCGTGCATTCGCCGTTCGGCCTGTCGCTGCGCGCGATCAAGAACAATCCGCTGCGCGCGTCGGCCATCG
GCATTCCCGTCAACAAGCGCCTGATCGCGATCTATACCGTCGCGGCGTTCTATGCCGGCATTGCCGGCGCGCTGTTCACG
CAGACGACGGCGCTCGCCTCGCTCGACGTGTTCTCGTTCGAGCGCTCCGCCGACCTCGTGCTGCTGCTGGTCATCGGCGG
CTCCGGCTATCTCTATGGCGGCCTGATCGGCGCGGCCGTGTTCCGGATGCTGCAGGAGCTGTTCCAGTGGATCACGCCGC
AATACTGGATGTTCTGGATCGGCCTCGTGCTGGTCGTGATCGTCATGGTCGGCCGCCATCGCATCCACGCCTATGCGCTG
TGGTTGCCCAATCTGATCCTGAAGCAGATCGCCGGCCGCAAGGCCGCGGTTGTCCTCACCGACAACGAGGCGCCATGA

Upstream 100 bases:

>100_bases
CCGAAGCTCGGCCCTTTCGTCATCTACACCATCATGATCGTGATCCTGATCTGGCGTCCGAACGGCCTGTTCGGTCGCAC
GGCGACGCGGTGAGGATCGC

Downstream 100 bases:

>100_bases
CCTACGCATTGGAAACGCGCGGCCTCGACAAGCATTTCGGCGGACTTCACGTCACTCGCAATCTGTCGCTGCAGATCAGG
CCCGGCGCGCGGCATGCGCT

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTTNAQTEVGFHARQHGRWHWSEFAFWIVVLAVGLAVPSRYLIMTEILRLALFALSLDLILGYAGIVSLGHAAFFGVGAY
CAGLLSVHGIINEPVLALIVAGLAATVLGFATSFLVIRGVDLTRLMVTLGIALLMEALAERFSNITGGTDGLQGIDIQPI
FGLFAFDMFGRTGFFYSLAVLFVLFLLARMIVHSPFGLSLRAIKNNPLRASAIGIPVNKRLIAIYTVAAFYAGIAGALFT
QTTALASLDVFSFERSADLVLLLVIGGSGYLYGGLIGAAVFRMLQELFQWITPQYWMFWIGLVLVVIVMVGRHRIHAYAL
WLPNLILKQIAGRKAAVVLTDNEAP

Sequences:

>Translated_345_residues
MTTNAQTEVGFHARQHGRWHWSEFAFWIVVLAVGLAVPSRYLIMTEILRLALFALSLDLILGYAGIVSLGHAAFFGVGAY
CAGLLSVHGIINEPVLALIVAGLAATVLGFATSFLVIRGVDLTRLMVTLGIALLMEALAERFSNITGGTDGLQGIDIQPI
FGLFAFDMFGRTGFFYSLAVLFVLFLLARMIVHSPFGLSLRAIKNNPLRASAIGIPVNKRLIAIYTVAAFYAGIAGALFT
QTTALASLDVFSFERSADLVLLLVIGGSGYLYGGLIGAAVFRMLQELFQWITPQYWMFWIGLVLVVIVMVGRHRIHAYAL
WLPNLILKQIAGRKAAVVLTDNEAP
>Mature_344_residues
TTNAQTEVGFHARQHGRWHWSEFAFWIVVLAVGLAVPSRYLIMTEILRLALFALSLDLILGYAGIVSLGHAAFFGVGAYC
AGLLSVHGIINEPVLALIVAGLAATVLGFATSFLVIRGVDLTRLMVTLGIALLMEALAERFSNITGGTDGLQGIDIQPIF
GLFAFDMFGRTGFFYSLAVLFVLFLLARMIVHSPFGLSLRAIKNNPLRASAIGIPVNKRLIAIYTVAAFYAGIAGALFTQ
TTALASLDVFSFERSADLVLLLVIGGSGYLYGGLIGAAVFRMLQELFQWITPQYWMFWIGLVLVVIVMVGRHRIHAYALW
LPNLILKQIAGRKAAVVLTDNEAP

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=305, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37479; Mature: 37348

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNAQTEVGFHARQHGRWHWSEFAFWIVVLAVGLAVPSRYLIMTEILRLALFALSLDLI
CCCCCCCHHCHHHHHCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGYAGIVSLGHAAFFGVGAYCAGLLSVHGIINEPVLALIVAGLAATVLGFATSFLVIRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLTRLMVTLGIALLMEALAERFSNITGGTDGLQGIDIQPIFGLFAFDMFGRTGFFYSLAV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
LFVLFLLARMIVHSPFGLSLRAIKNNPLRASAIGIPVNKRLIAIYTVAAFYAGIAGALFT
HHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QTTALASLDVFSFERSADLVLLLVIGGSGYLYGGLIGAAVFRMLQELFQWITPQYWMFWI
HHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
GLVLVVIVMVGRHRIHAYALWLPNLILKQIAGRKAAVVLTDNEAP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure 
TTNAQTEVGFHARQHGRWHWSEFAFWIVVLAVGLAVPSRYLIMTEILRLALFALSLDLI
CCCCCCHHCHHHHHCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGYAGIVSLGHAAFFGVGAYCAGLLSVHGIINEPVLALIVAGLAATVLGFATSFLVIRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLTRLMVTLGIALLMEALAERFSNITGGTDGLQGIDIQPIFGLFAFDMFGRTGFFYSLAV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
LFVLFLLARMIVHSPFGLSLRAIKNNPLRASAIGIPVNKRLIAIYTVAAFYAGIAGALFT
HHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QTTALASLDVFSFERSADLVLLLVIGGSGYLYGGLIGAAVFRMLQELFQWITPQYWMFWI
HHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
GLVLVVIVMVGRHRIHAYALWLPNLILKQIAGRKAAVVLTDNEAP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]