BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is braF [H]

Identifier: 146342107

GI number: 146342107

Start: 5463003

End: 5463749

Strand: Reverse

Name: braF [H]

Synonym: BRADO5251

Alternate gene names: 146342107

Gene position: 5463749-5463003 (Counterclockwise)

Preceding gene: 146342108

Following gene: 146342106

Centisome position: 73.27

GC content: 67.07

Gene sequence:

>747_bases
ATGACCTACGCATTGGAAACGCGCGGCCTCGACAAGCATTTCGGCGGACTTCACGTCACTCGCAATCTGTCGCTGCAGAT
CAGGCCCGGCGCGCGGCATGCGCTGATCGGCCCGAACGGCGCCGGCAAGACCACCGTGATCAATCAGCTGACCGGCGTGC
TCGAGCCGAGTTCGGGGCGCATTCTGCTCGAAGGCCAGGACATCACCGACCTTCCGGTGCACAAGCGCGTGCTGCGCGGC
CTGTCGCGGACCTTCCAGATCAATCAGCTCTATCCGGATCTGACTCCGCTCGAGACCATCGGGCTTGCGGTGTCGGAGCG
GCTCGGCGGCGGCGGCAATTGGTGGCGACGCATGGGCACGCGCGACGACATCAATGCCGAGATCGCCGACACCCTGGAGC
GCTTCCGCCTGCTCGACGTGATGAACGACCGGACCTCGACGCTTCCCTATGGCAAGCAGCGCCTGCTGGAGATCGCGGTT
GCGATCGCCGCCAAGCCGCGCGTGCTGCTGCTCGACGAGCCGGCCGCCGGCGTGCCCGAGGGCGAGCGCAAGGACATTCT
CGCCGCCGTCGCCGCGCTGCCGCGCGACGTCACGGTGCTCTTGATCGAACACGACATGGACCTCGTCTTCTCCTTCGCCG
ACCGCATCTCGGTTCTCGTCTCCGGCGCTCTTCTGGTGGAGGGAGCCCCCGACGAGGTTGCGCGCGATCCGCAGGTCAAG
GCGGTCTATCTCGGCGAGGCCGCGTGA

Upstream 100 bases:

>100_bases
GCCATCGCATCCACGCCTATGCGCTGTGGTTGCCCAATCTGATCCTGAAGCAGATCGCCGGCCGCAAGGCCGCGGTTGTC
CTCACCGACAACGAGGCGCC

Downstream 100 bases:

>100_bases
TGACCGATCTGCTCGCCATCGAGAATCTCCGCGCCGGCTATGGCCAGGCGGTCGTTTTGCCGAACATGTCCTTGAGGCTG
CCGGAAGGCCAGGTACTGGC

Product: branched chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTYALETRGLDKHFGGLHVTRNLSLQIRPGARHALIGPNGAGKTTVINQLTGVLEPSSGRILLEGQDITDLPVHKRVLRG
LSRTFQINQLYPDLTPLETIGLAVSERLGGGGNWWRRMGTRDDINAEIADTLERFRLLDVMNDRTSTLPYGKQRLLEIAV
AIAAKPRVLLLDEPAAGVPEGERKDILAAVAALPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGAPDEVARDPQVK
AVYLGEAA

Sequences:

>Translated_248_residues
MTYALETRGLDKHFGGLHVTRNLSLQIRPGARHALIGPNGAGKTTVINQLTGVLEPSSGRILLEGQDITDLPVHKRVLRG
LSRTFQINQLYPDLTPLETIGLAVSERLGGGGNWWRRMGTRDDINAEIADTLERFRLLDVMNDRTSTLPYGKQRLLEIAV
AIAAKPRVLLLDEPAAGVPEGERKDILAAVAALPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGAPDEVARDPQVK
AVYLGEAA
>Mature_247_residues
TYALETRGLDKHFGGLHVTRNLSLQIRPGARHALIGPNGAGKTTVINQLTGVLEPSSGRILLEGQDITDLPVHKRVLRGL
SRTFQINQLYPDLTPLETIGLAVSERLGGGGNWWRRMGTRDDINAEIADTLERFRLLDVMNDRTSTLPYGKQRLLEIAVA
IAAKPRVLLLDEPAAGVPEGERKDILAAVAALPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGAPDEVARDPQVKA
VYLGEAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=231, Percent_Identity=32.4675324675325, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI30795238, Length=231, Percent_Identity=32.4675324675325, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI153792144, Length=213, Percent_Identity=31.924882629108, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI116734710, Length=209, Percent_Identity=33.0143540669856, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI31657092, Length=213, Percent_Identity=30.9859154929577, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI6005701, Length=215, Percent_Identity=32.5581395348837, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI105990541, Length=198, Percent_Identity=32.3232323232323, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI27262624, Length=207, Percent_Identity=29.4685990338164, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI27262626, Length=207, Percent_Identity=29.4685990338164, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI47078218, Length=212, Percent_Identity=30.6603773584906, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45446740, Length=212, Percent_Identity=30.6603773584906, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI27436953, Length=212, Percent_Identity=28.7735849056604, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI27477115, Length=214, Percent_Identity=29.9065420560748, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI150417984, Length=232, Percent_Identity=29.7413793103448, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI21536376, Length=213, Percent_Identity=30.9859154929577, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI11967971, Length=225, Percent_Identity=25.3333333333333, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI11967969, Length=226, Percent_Identity=25.6637168141593, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4505771, Length=241, Percent_Identity=23.6514522821577, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592898, Length=212, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592964, Length=212, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592956, Length=212, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592915, Length=212, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI9961252, Length=241, Percent_Identity=23.6514522821577, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592971, Length=212, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592978, Length=212, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI171184400, Length=228, Percent_Identity=27.6315789473684, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=40.4761904761905, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1789672, Length=232, Percent_Identity=28.8793103448276, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789593, Length=249, Percent_Identity=32.5301204819277, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081709, Length=248, Percent_Identity=27.4193548387097, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787370, Length=242, Percent_Identity=29.7520661157025, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081791, Length=243, Percent_Identity=27.9835390946502, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082268, Length=235, Percent_Identity=30.6382978723404, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788472, Length=227, Percent_Identity=28.1938325991189, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788761, Length=249, Percent_Identity=30.1204819277108, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081782, Length=230, Percent_Identity=30.4347826086957, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787029, Length=235, Percent_Identity=26.3829787234043, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI48994943, Length=255, Percent_Identity=32.156862745098, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790525, Length=236, Percent_Identity=27.1186440677966, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786319, Length=228, Percent_Identity=30.7017543859649, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790739, Length=252, Percent_Identity=28.1746031746032, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787712, Length=244, Percent_Identity=26.6393442622951, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788210, Length=220, Percent_Identity=28.1818181818182, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48995001, Length=243, Percent_Identity=27.5720164609054, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1790190, Length=230, Percent_Identity=27.3913043478261, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790162, Length=245, Percent_Identity=24.8979591836735, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789873, Length=215, Percent_Identity=29.3023255813954, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789991, Length=227, Percent_Identity=29.0748898678414, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790467, Length=236, Percent_Identity=24.5762711864407, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787792, Length=243, Percent_Identity=27.9835390946502, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786398, Length=221, Percent_Identity=27.1493212669683, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788225, Length=247, Percent_Identity=28.7449392712551, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=30.8755760368664, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786345, Length=248, Percent_Identity=29.0322580645161, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786803, Length=232, Percent_Identity=24.1379310344828, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=26.5822784810127, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1789032, Length=224, Percent_Identity=26.3392857142857, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787548, Length=226, Percent_Identity=28.3185840707965, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788450, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693107, Length=227, Percent_Identity=30.8370044052863, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787089, Length=244, Percent_Identity=25.8196721311475, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788165, Length=245, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081834, Length=197, Percent_Identity=27.9187817258883, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082267, Length=225, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786872, Length=233, Percent_Identity=24.0343347639485, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI48994997, Length=223, Percent_Identity=26.9058295964126, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787547, Length=265, Percent_Identity=23.7735849056604, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786698, Length=225, Percent_Identity=25.7777777777778, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787759, Length=235, Percent_Identity=26.8085106382979, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1789962, Length=228, Percent_Identity=23.6842105263158, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1787758, Length=238, Percent_Identity=24.7899159663866, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=213, Percent_Identity=30.5164319248826, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983516, Length=213, Percent_Identity=30.5164319248826, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983523, Length=213, Percent_Identity=30.5164319248826, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565586, Length=210, Percent_Identity=30, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17510237, Length=226, Percent_Identity=29.646017699115, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115533608, Length=222, Percent_Identity=32.4324324324324, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193209708, Length=229, Percent_Identity=26.2008733624454, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193208177, Length=229, Percent_Identity=26.2008733624454, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=223, Percent_Identity=23.7668161434978, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533592, Length=225, Percent_Identity=28.4444444444444, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24666092, Length=227, Percent_Identity=28.6343612334802, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI116007184, Length=227, Percent_Identity=30.3964757709251, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI221500365, Length=227, Percent_Identity=30.3964757709251, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24643674, Length=227, Percent_Identity=30.8370044052863, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221512771, Length=219, Percent_Identity=28.7671232876712, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI85724858, Length=201, Percent_Identity=31.3432835820896, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI85724856, Length=201, Percent_Identity=31.3432835820896, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580547, Length=201, Percent_Identity=31.3432835820896, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580551, Length=201, Percent_Identity=31.3432835820896, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580549, Length=201, Percent_Identity=31.3432835820896, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580545, Length=201, Percent_Identity=31.3432835820896, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571209, Length=232, Percent_Identity=25, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136662, Length=216, Percent_Identity=26.8518518518519, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26977; Mature: 26846

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYALETRGLDKHFGGLHVTRNLSLQIRPGARHALIGPNGAGKTTVINQLTGVLEPSSGR
CCEEECCCCCCHHCCCEEEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCE
ILLEGQDITDLPVHKRVLRGLSRTFQINQLYPDLTPLETIGLAVSERLGGGGNWWRRMGT
EEEECCCCCCCHHHHHHHHHHHHEEEEHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCC
RDDINAEIADTLERFRLLDVMNDRTSTLPYGKQRLLEIAVAIAAKPRVLLLDEPAAGVPE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
GERKDILAAVAALPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGAPDEVARDPQVK
CCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCEE
AVYLGEAA
EEEECCCC
>Mature Secondary Structure 
TYALETRGLDKHFGGLHVTRNLSLQIRPGARHALIGPNGAGKTTVINQLTGVLEPSSGR
CEEECCCCCCHHCCCEEEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCE
ILLEGQDITDLPVHKRVLRGLSRTFQINQLYPDLTPLETIGLAVSERLGGGGNWWRRMGT
EEEECCCCCCCHHHHHHHHHHHHEEEEHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCC
RDDINAEIADTLERFRLLDVMNDRTSTLPYGKQRLLEIAVAIAAKPRVLLLDEPAAGVPE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
GERKDILAAVAALPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGAPDEVARDPQVK
CCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCEE
AVYLGEAA
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]