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The map label for this gene is cysD

Identifier: 146342052

GI number: 146342052

Start: 5402486

End: 5403418

Strand: Direct

Name: cysD

Synonym: BRADO5192

Alternate gene names: 146342052

Gene position: 5402486-5403418 (Clockwise)

Preceding gene: 146342051

Following gene: 146342053

Centisome position: 72.45

GC content: 56.38

Gene sequence:

>933_bases
ATGACGATGGATCAGGTCCCGATATCACGTTCGCAGCTCCCCGCACATCTGAAGCGGCTCGAATCCGAATCGATCGAGAT
CATCCGCGAGGTTGCAGCAGAGCTCAAGAATCCCGTGATGCTGTATTCCATAGGCAAAGATTCCGGGGTCATGCTCCACA
TTGCGTTCAAGGCTTTTTTCCCCGGACGCCTTCCGTTTCCACTGCTGCACGTCGATACGACGTGGAAGTTCAGGGACATG
ATCATGTTCCGGGATGAGCTGGCCAAGAAACTCGGTTTCGACCTGCTGGTTCATACGAATGAAGACGGCCTGCGCGCGGG
CATAAATCCTTTTACCTCGGGCCCCTCGCTGCACACGCACGTGATGAAGACGGAGGCGCTGAAGCAGGCGCTCGATTTGC
ATGGTTTCGACGCCGCATTTGGCGGCGCCCGTCGAGACGAGGAGAAGAGTCGAGCCAAAGAACGGATCTTTTCGTTTCGA
GCGGAAGGTCACGTTTGGGAGCCGCGCAATCAGCGTCCCGAGCTTTGGAATTTGTTCAACACACGTATTAGGCCGGGCGA
ATCGCTCCGCGTATTCCCACTCTCGAACTGGACGGAGTTCGATGTCTGGCACTACATATCTCTCGAAAGGATTCCGGTCG
TCCCACTCTATTTCGCTAAGGAACGTCCGGTCGTCTGGCGCGATGGCACCTGGATCATGGTTGACGACGCGCGCTTCCCG
CTGGCGCCTGGCGAGACACCGCAGCTTCGCAAGGTCCGCTTTAGAACACTCGGCTGTTATCCTCTGACAGGCGCCATAGA
GTCGGCTGCAACATCGGTCGAAGACATCCTCGATGAAATGAGGACTACGCGTATCTCGGAGCGTCAGGGACGCCTCATTG
ATACGGACGAGGCCGCATCCATGGAGAAAAAGAAGAAAGAGGGCTACTTCTGA

Upstream 100 bases:

>100_bases
GCCTGACAGCAGACCAGATCGATTTCATAGCTGACGAGCTGACGGACGCGCTGAAGTAGGTAAGCTCTGGATCGATGAGT
CAGCTCAAACCGCAATACAG

Downstream 100 bases:

>100_bases
TGGCTGCAGCTCAGGCACCAGGGGCGCCGTCCAGAGACCTTCTGCGCTTCATCACCTGCGGTTCGGTCGATGACGGAAAA
TCGACACTCATCGGACGCCT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTMDQVPISRSQLPAHLKRLESESIEIIREVAAELKNPVMLYSIGKDSGVMLHIAFKAFFPGRLPFPLLHVDTTWKFRDM
IMFRDELAKKLGFDLLVHTNEDGLRAGINPFTSGPSLHTHVMKTEALKQALDLHGFDAAFGGARRDEEKSRAKERIFSFR
AEGHVWEPRNQRPELWNLFNTRIRPGESLRVFPLSNWTEFDVWHYISLERIPVVPLYFAKERPVVWRDGTWIMVDDARFP
LAPGETPQLRKVRFRTLGCYPLTGAIESAATSVEDILDEMRTTRISERQGRLIDTDEAASMEKKKKEGYF

Sequences:

>Translated_310_residues
MTMDQVPISRSQLPAHLKRLESESIEIIREVAAELKNPVMLYSIGKDSGVMLHIAFKAFFPGRLPFPLLHVDTTWKFRDM
IMFRDELAKKLGFDLLVHTNEDGLRAGINPFTSGPSLHTHVMKTEALKQALDLHGFDAAFGGARRDEEKSRAKERIFSFR
AEGHVWEPRNQRPELWNLFNTRIRPGESLRVFPLSNWTEFDVWHYISLERIPVVPLYFAKERPVVWRDGTWIMVDDARFP
LAPGETPQLRKVRFRTLGCYPLTGAIESAATSVEDILDEMRTTRISERQGRLIDTDEAASMEKKKKEGYF
>Mature_309_residues
TMDQVPISRSQLPAHLKRLESESIEIIREVAAELKNPVMLYSIGKDSGVMLHIAFKAFFPGRLPFPLLHVDTTWKFRDMI
MFRDELAKKLGFDLLVHTNEDGLRAGINPFTSGPSLHTHVMKTEALKQALDLHGFDAAFGGARRDEEKSRAKERIFSFRA
EGHVWEPRNQRPELWNLFNTRIRPGESLRVFPLSNWTEFDVWHYISLERIPVVPLYFAKERPVVWRDGTWIMVDDARFPL
APGETPQLRKVRFRTLGCYPLTGAIESAATSVEDILDEMRTTRISERQGRLIDTDEAASMEKKKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=308, Percent_Identity=64.6103896103896, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_BRASO (A4YYA7)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001207100.1
- ProteinModelPortal:   A4YYA7
- SMR:   A4YYA7
- STRING:   A4YYA7
- GeneID:   5116117
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO5192
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   HYGFDAA
- ProtClustDB:   PRK12563
- BioCyc:   BSP376:BRADO5192-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 35771; Mature: 35639

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDQVPISRSQLPAHLKRLESESIEIIREVAAELKNPVMLYSIGKDSGVMLHIAFKAFF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEHHC
PGRLPFPLLHVDTTWKFRDMIMFRDELAKKLGFDLLVHTNEDGLRAGINPFTSGPSLHTH
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
VMKTEALKQALDLHGFDAAFGGARRDEEKSRAKERIFSFRAEGHVWEPRNQRPELWNLFN
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHH
TRIRPGESLRVFPLSNWTEFDVWHYISLERIPVVPLYFAKERPVVWRDGTWIMVDDARFP
CCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCEEEEECCCCC
LAPGETPQLRKVRFRTLGCYPLTGAIESAATSVEDILDEMRTTRISERQGRLIDTDEAAS
CCCCCCCHHHHHHHHEECCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHH
MEKKKKEGYF
HHHHHHCCCC
>Mature Secondary Structure 
TMDQVPISRSQLPAHLKRLESESIEIIREVAAELKNPVMLYSIGKDSGVMLHIAFKAFF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEHHC
PGRLPFPLLHVDTTWKFRDMIMFRDELAKKLGFDLLVHTNEDGLRAGINPFTSGPSLHTH
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
VMKTEALKQALDLHGFDAAFGGARRDEEKSRAKERIFSFRAEGHVWEPRNQRPELWNLFN
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHH
TRIRPGESLRVFPLSNWTEFDVWHYISLERIPVVPLYFAKERPVVWRDGTWIMVDDARFP
CCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCEEEEECCCCC
LAPGETPQLRKVRFRTLGCYPLTGAIESAATSVEDILDEMRTTRISERQGRLIDTDEAAS
CCCCCCCHHHHHHHHEECCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHH
MEKKKKEGYF
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA