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The map label for this gene is rrmA [C]

Identifier: 146342036

GI number: 146342036

Start: 5383906

End: 5384568

Strand: Direct

Name: rrmA [C]

Synonym: BRADO5174

Alternate gene names: 146342036

Gene position: 5383906-5384568 (Clockwise)

Preceding gene: 146342035

Following gene: 146342037

Centisome position: 72.2

GC content: 61.39

Gene sequence:

>663_bases
ATGGCCTATCGCGAGTTCGTCAGTCTCGTCCACAAATCGACCAAGCGCGACTATCTCGCGCGCGTCACCCAGCGCGACAA
GGCCGATGTCGCTGAAATGGCGATCAAGTACGACTACGATTATTGGGACGGCAGCCGCGAGACCGGCTATGGCGGCTACA
AATATGACGGCCGCTGGCGCAAGGTCGCCGACGCGATGATCGCGACCTACGGCATCAAGCCCGGCATGCGCGTGCTCGAC
GTCGGCTCCGGCAAGGGTTTCATCCTGCACGATTTCCGCGAGGCCATTCCGGACCTCGAGGTCGCCGGCATCGACATCTC
CTCCTACGGCGTCGAGCATACGATGGAGAGCGTGAAGCCGTTCTGCCAGGTCGGCTCCGCCGCCAAGCTGCCCTACCCGG
ACAAGCACTTCGACCTCGTCATCTCGATCAACACGCTGCACAACCTCTACAACTACGATCTCGACGCTGCGCTGCGCGAG
ATGGAGCGTGTCAGCCGCGGCGCCAAGTATCTCTGCGTCGAGGGATACCGCAACGAGCGCGAGAAGGTGAACCTGATGTA
CTGGCAGCTCACCTGCCGCATCTTCCACACCCCCGAGGAATGGGCCTTCGAATTCAAGCGCTCCGGCTACACCGGCGACC
ACGAGTTCATCTTCTTCGAATGA

Upstream 100 bases:

>100_bases
GGCGCAGCGCTGCGCCGCGCAGCTCGCCAAGGCCGAAATACAGCTCGGCCTGATCGGCGCCACATCCGATTAACCGACAC
TCACACGCCCGGAGAGCTTC

Downstream 100 bases:

>100_bases
GCTGAAGTTTTCACAGCCTCGGTGGCGCCGCTGTCAAATCGACGCCAAGTGCGGCGCTACTGTGGCCATGCTTGGCGCTT
TTGCCGCGCCATTGTTAGTC

Product: putative S-adenosyl-L-methionine (SAM)-dependent methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: Methyltransferase Type; SAM-Dependent Methyltransferase; Protein-L-Isoaspartate(D-Aspartate) O-Methyltransferase; S-Adenosyl-L-Methionine-Dependent Methyltransferase

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAYREFVSLVHKSTKRDYLARVTQRDKADVAEMAIKYDYDYWDGSRETGYGGYKYDGRWRKVADAMIATYGIKPGMRVLD
VGSGKGFILHDFREAIPDLEVAGIDISSYGVEHTMESVKPFCQVGSAAKLPYPDKHFDLVISINTLHNLYNYDLDAALRE
MERVSRGAKYLCVEGYRNEREKVNLMYWQLTCRIFHTPEEWAFEFKRSGYTGDHEFIFFE

Sequences:

>Translated_220_residues
MAYREFVSLVHKSTKRDYLARVTQRDKADVAEMAIKYDYDYWDGSRETGYGGYKYDGRWRKVADAMIATYGIKPGMRVLD
VGSGKGFILHDFREAIPDLEVAGIDISSYGVEHTMESVKPFCQVGSAAKLPYPDKHFDLVISINTLHNLYNYDLDAALRE
MERVSRGAKYLCVEGYRNEREKVNLMYWQLTCRIFHTPEEWAFEFKRSGYTGDHEFIFFE
>Mature_219_residues
AYREFVSLVHKSTKRDYLARVTQRDKADVAEMAIKYDYDYWDGSRETGYGGYKYDGRWRKVADAMIATYGIKPGMRVLDV
GSGKGFILHDFREAIPDLEVAGIDISSYGVEHTMESVKPFCQVGSAAKLPYPDKHFDLVISINTLHNLYNYDLDAALREM
ERVSRGAKYLCVEGYRNEREKVNLMYWQLTCRIFHTPEEWAFEFKRSGYTGDHEFIFFE

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 25617; Mature: 25486

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYREFVSLVHKSTKRDYLARVTQRDKADVAEMAIKYDYDYWDGSRETGYGGYKYDGRWR
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCCEEECCHHH
KVADAMIATYGIKPGMRVLDVGSGKGFILHDFREAIPDLEVAGIDISSYGVEHTMESVKP
HHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHCCCCEEEEEEHHHHCHHHHHHHHHH
FCQVGSAAKLPYPDKHFDLVISINTLHNLYNYDLDAALREMERVSRGAKYLCVEGYRNER
HHHCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
EKVNLMYWQLTCRIFHTPEEWAFEFKRSGYTGDHEFIFFE
HHEEEEEEEEEEEEEECCHHHHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure 
AYREFVSLVHKSTKRDYLARVTQRDKADVAEMAIKYDYDYWDGSRETGYGGYKYDGRWR
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCCEEECCHHH
KVADAMIATYGIKPGMRVLDVGSGKGFILHDFREAIPDLEVAGIDISSYGVEHTMESVKP
HHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHCCCCEEEEEEHHHHCHHHHHHHHHH
FCQVGSAAKLPYPDKHFDLVISINTLHNLYNYDLDAALREMERVSRGAKYLCVEGYRNER
HHHCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
EKVNLMYWQLTCRIFHTPEEWAFEFKRSGYTGDHEFIFFE
HHEEEEEEEEEEEEEECCHHHHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA