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The map label for this gene is frmA [H]

Identifier: 146342033

GI number: 146342033

Start: 5381187

End: 5382224

Strand: Direct

Name: frmA [H]

Synonym: BRADO5171

Alternate gene names: 146342033

Gene position: 5381187-5382224 (Clockwise)

Preceding gene: 146342032

Following gene: 146342034

Centisome position: 72.17

GC content: 68.3

Gene sequence:

>1038_bases
ATGAAGACCCAAGCTGCCCTCCTGGTCCAGACCGGCCAGCCGCTCGTGCTCGCCGAGATCGAGACCCCTGCCTTGAAGCC
GGGCCAGGTGCTGGTCGAGATCGCCTATTCCGGCGCCTGCGGCACGCAGGTGATGGAGTGGCGCGGCGACAAGGGCGAGG
ACAAATGGGTGCCGCATTGCCTCGGACACGAGGGCACCGGCACGGTCATCGAAACCGGCAGCGCCGTCACCAAGGTCAAG
GTCGGCGACAAGGTCGTGCTGTCCTGGATCAAGGGCACGGGCATCGAGGCCGGCGGCGCCGTCTATGACTGGGACGGCAA
GAAGGTGAATGCCGGCGGCGTCACCACCTTCCAGCGCCATTCGGTGGTCAGCGAGAACCGCCTAACCCTGCTGCCGCCGG
ACCTGCCGATGGACGTCGCGGTGCTGCTCGGCTGCGCCGCGCCGACCGGGATGGGCGCGGTCTACAACGTGCTGAAGGTG
CAGCCGGGCGACGCCGTCGCGGTGTTCGGCACCGGCGGCATCGGCCTCAACGCGCTGATGGCAGCTAGCCTCGCGGGCGC
GATGCCGGTGATCGGCATCGACCCCAACCCGACCCGCCGCGCGCTCGCCAAGCTCTACGGCGCAACTCACGTGATCGACG
CCTCCGGCGACGTGCTGGCCGAGATCAAGAAGATCGTGCCGCAGGGTGTTGATCTCGCGGTCGAATCTTCCGGCATCCCC
GGCGTGATGGATCAGGCCGTCAATGCCACCCGCCAGCAGGGCGGCCGCGCGGTCGTGATCGGCAATGCCAAGCATGGCGC
GGCGCTGACCTTGAACCCCGGCGTCTTCAACCATGGCAAGAGCCTGCTCGGCACCTGGGGCGGCGACAGCGTGCCGGATC
GCGATTACGGGCGCTACGGGCGTCTTCTGAGCTCTGGCCGCTTCCCGGTGCGCGACCTCCTGTCGAAACCCTACAGTCTC
GCCCAGGCCGACCAGGCGCTGCAGGATCTCGCCGCCGGCAAGGTCGGCCGTCCGCTGATCGACATGTCCTTGCGTTAA

Upstream 100 bases:

>100_bases
TCAAGAAATCCGGTGAACAGGAATATGCCCGCGAGGTGCTCGGCCTGACCGGCCACCAGATCGCGGACAAGATCATCCAC
GCCTTGAGCTGAGACATCAC

Downstream 100 bases:

>100_bases
GTTGAGGCCATGCGGATCGGGATCGACTTCGACAACACGATCGCCTGCTATGACGGCGTGTTTCATGCGGCAGCGCTGGA
GCGCGGCCTGATCCCGGCCG

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKTQAALLVQTGQPLVLAEIETPALKPGQVLVEIAYSGACGTQVMEWRGDKGEDKWVPHCLGHEGTGTVIETGSAVTKVK
VGDKVVLSWIKGTGIEAGGAVYDWDGKKVNAGGVTTFQRHSVVSENRLTLLPPDLPMDVAVLLGCAAPTGMGAVYNVLKV
QPGDAVAVFGTGGIGLNALMAASLAGAMPVIGIDPNPTRRALAKLYGATHVIDASGDVLAEIKKIVPQGVDLAVESSGIP
GVMDQAVNATRQQGGRAVVIGNAKHGAALTLNPGVFNHGKSLLGTWGGDSVPDRDYGRYGRLLSSGRFPVRDLLSKPYSL
AQADQALQDLAAGKVGRPLIDMSLR

Sequences:

>Translated_345_residues
MKTQAALLVQTGQPLVLAEIETPALKPGQVLVEIAYSGACGTQVMEWRGDKGEDKWVPHCLGHEGTGTVIETGSAVTKVK
VGDKVVLSWIKGTGIEAGGAVYDWDGKKVNAGGVTTFQRHSVVSENRLTLLPPDLPMDVAVLLGCAAPTGMGAVYNVLKV
QPGDAVAVFGTGGIGLNALMAASLAGAMPVIGIDPNPTRRALAKLYGATHVIDASGDVLAEIKKIVPQGVDLAVESSGIP
GVMDQAVNATRQQGGRAVVIGNAKHGAALTLNPGVFNHGKSLLGTWGGDSVPDRDYGRYGRLLSSGRFPVRDLLSKPYSL
AQADQALQDLAAGKVGRPLIDMSLR
>Mature_345_residues
MKTQAALLVQTGQPLVLAEIETPALKPGQVLVEIAYSGACGTQVMEWRGDKGEDKWVPHCLGHEGTGTVIETGSAVTKVK
VGDKVVLSWIKGTGIEAGGAVYDWDGKKVNAGGVTTFQRHSVVSENRLTLLPPDLPMDVAVLLGCAAPTGMGAVYNVLKV
QPGDAVAVFGTGGIGLNALMAASLAGAMPVIGIDPNPTRRALAKLYGATHVIDASGDVLAEIKKIVPQGVDLAVESSGIP
GVMDQAVNATRQQGGRAVVIGNAKHGAALTLNPGVFNHGKSLLGTWGGDSVPDRDYGRYGRLLSSGRFPVRDLLSKPYSL
AQADQALQDLAAGKVGRPLIDMSLR

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=26.9021739130435, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI71565154, Length=378, Percent_Identity=30.4232804232804, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4501939, Length=362, Percent_Identity=27.0718232044199, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI71565152, Length=380, Percent_Identity=29.4736842105263, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI4501929, Length=368, Percent_Identity=27.1739130434783, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI34577061, Length=350, Percent_Identity=29.1428571428571, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4501933, Length=350, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI71743840, Length=351, Percent_Identity=29.9145299145299, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI262073058, Length=367, Percent_Identity=27.5204359673025, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786552, Length=304, Percent_Identity=34.8684210526316, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082125, Length=367, Percent_Identity=25.8855585831063, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788073, Length=356, Percent_Identity=27.247191011236, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788407, Length=331, Percent_Identity=25.3776435045317, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790045, Length=363, Percent_Identity=23.6914600550964, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081918, Length=325, Percent_Identity=28.3076923076923, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=379, Percent_Identity=28.7598944591029, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71997431, Length=381, Percent_Identity=28.6089238845144, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17565904, Length=305, Percent_Identity=32.1311475409836, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71988145, Length=354, Percent_Identity=27.1186440677966, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562584, Length=358, Percent_Identity=26.2569832402235, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=373, Percent_Identity=29.2225201072386, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319621, Length=366, Percent_Identity=28.1420765027322, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=351, Percent_Identity=26.2108262108262, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=361, Percent_Identity=24.9307479224377, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=254, Percent_Identity=27.9527559055118, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=368, Percent_Identity=25.8152173913043, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17737895, Length=377, Percent_Identity=28.6472148541114, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 35753; Mature: 35753

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQAALLVQTGQPLVLAEIETPALKPGQVLVEIAYSGACGTQVMEWRGDKGEDKWVPHC
CCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCHH
LGHEGTGTVIETGSAVTKVKVGDKVVLSWIKGTGIEAGGAVYDWDGKKVNAGGVTTFQRH
CCCCCCCCEEECCCCEEEEECCCCEEEEEHHCCCCCCCCEEEECCCCEECCCCCCEEHHH
SVVSENRLTLLPPDLPMDVAVLLGCAAPTGMGAVYNVLKVQPGDAVAVFGTGGIGLNALM
HHHCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHHHEEEECCCCEEEEEECCCCCHHHHH
AASLAGAMPVIGIDPNPTRRALAKLYGATHVIDASGDVLAEIKKIVPQGVDLAVESSGIP
HHHHHCCCCEEECCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEECCCCC
GVMDQAVNATRQQGGRAVVIGNAKHGAALTLNPGVFNHGKSLLGTWGGDSVPDRDYGRYG
HHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCHHEEECCCCCCCCCCHHHHH
RLLSSGRFPVRDLLSKPYSLAQADQALQDLAAGKVGRPLIDMSLR
HHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTQAALLVQTGQPLVLAEIETPALKPGQVLVEIAYSGACGTQVMEWRGDKGEDKWVPHC
CCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCHH
LGHEGTGTVIETGSAVTKVKVGDKVVLSWIKGTGIEAGGAVYDWDGKKVNAGGVTTFQRH
CCCCCCCCEEECCCCEEEEECCCCEEEEEHHCCCCCCCCEEEECCCCEECCCCCCEEHHH
SVVSENRLTLLPPDLPMDVAVLLGCAAPTGMGAVYNVLKVQPGDAVAVFGTGGIGLNALM
HHHCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHHHEEEECCCCEEEEEECCCCCHHHHH
AASLAGAMPVIGIDPNPTRRALAKLYGATHVIDASGDVLAEIKKIVPQGVDLAVESSGIP
HHHHHCCCCEEECCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEECCCCC
GVMDQAVNATRQQGGRAVVIGNAKHGAALTLNPGVFNHGKSLLGTWGGDSVPDRDYGRYG
HHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCHHEEECCCCCCCCCCHHHHH
RLLSSGRFPVRDLLSKPYSLAQADQALQDLAAGKVGRPLIDMSLR
HHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]