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The map label for this gene is nfdA [H]

Identifier: 146341990

GI number: 146341990

Start: 5325475

End: 5327352

Strand: Reverse

Name: nfdA [H]

Synonym: BRADO5126

Alternate gene names: 146341990

Gene position: 5327352-5325475 (Counterclockwise)

Preceding gene: 146341991

Following gene: 146341989

Centisome position: 71.44

GC content: 66.67

Gene sequence:

>1878_bases
ATGAGCGACCATCCCGATCTCATCCTGCATCGCGGCCTGTTCACGACGCTCGACCGCTCCAATCCGCAGGCGGATGCGGT
GGCGATCAAGGATGGCTTGTTCACCGCCGTCGGTCATGCGCAGGACGTCATGAAGCTCGCCGGCCCGCAGACGCAGGTGA
TCGATCTCAGGGGCCGGCGTGTGCTGCCGGGGCTGATCGATAATCACCTGCATATCATCCGCGGCGGGCTCAATTTTAAC
ATGGAGCTGCGCTGGGACGGCGTGCGCTCGCTCGCCGACGCGATGGCGATGCTGAAGGCGCAGGTCGCGATCACGCCGCC
GCCGCAATGGGTCCGCGTGGTCGGCGGCTTCACCGAGCATCAGTTCGCCGAGAAGCGGCTGCCGACGATCGATGAGCTGA
ACGCCGTGGCGCCGGAGACGCCGGTGTTCCTGCTGCATCTCTACGACCGCGCGATCCTCAACGGCGCCGCGCTGCGCGCC
GTCGGCTACGACAAGGACACGCCGCAGCCGCCGGGCGGCGAGATCGTGCGCGATGCCCAGGGCAATCCGACAGGGTTGCT
GCTCGCCAAGCCCAATGCCGCGATCCTCTACGCGACGCTGGCCAAGGGACCGAAGCTGCCGTTCGATTACCAGCTCAACT
CGACGCGCCACTTCATGCGCGAGCTGAACCGGCTCGGCGTCACCGGCGCGATCGATGCCGGCGGCGGCTTCCAGAACTAT
CCGGACGACTACGCCGTGATCCAGAAGCTCGCCGATGACGGCCAGCTCACCATCCGGCTCGCCTACAATCTGTTCACGCA
GAAGCCGAAGGGCGAGAAGGACGATTTCCTGCGCTGGACACAGAGCTCGGCCTACAAGCAGGGCAACGACTATTTCCGCC
ACAACGGCGCCGGCGAGATGCTGGTGTTCTCGGCCGCCGATTTCGAGGATTTCCGCGTGCCGCGGCCGGATATGCCGGCC
GAAATGGAGGGCGAGCTCGAGCAGGTCGTGCGCATCCTGGTGCAGAACAAATGGCCGTGGCGGCTGCACGCGACCTATGA
CGAGACCATCTCGCGGGCGCTCGATGTGTTCGAGAAGGTCAACCAGGACATGCCGCTCGAAGGGCTGCACTGGTTCTTCG
ACCATGCCGAGACGATCTCGGATCAGTCGATCGACCGCATCGCCGCGCTCGGCGGCGGCATCGCCGTGCAGCATCGCATG
GCCTATCAGGGCGAGTATTTCGTTGAACGCTATGGCTGTGGCGCCGCCGAGGCGACGCCACCGGTGAAGAAGATCCTGGA
CAAAGGCGTGAAGGTCTCCGCCGGCACCGACGCGACCCGCGTCGCGTCCTACAATCCCTGGGTCTCGCTGTCCTGGCTGG
TGACGGGGCGCACCGTCGGCGGCCTGCGGCTGTACCCGCAGCGCAATTGCCTCGACCGCGAGACGGCGCTGCGGATGTGG
ACGGAGAACGTCACCTGGTTCTCCAATGAGGAAGGCCGGAAGGGCCGCATCGCCGTCGGGCAGCTCGCCGATCTGATCGT
GCCGGATCGCGACTACTTCGCCTGCGCCGAGGTCGAGATCGCTGACATCACGAGCCATCTCACCATGGTCGGTGGCCGCA
TCGTCTACGCGGCGGCCGACTTCAAGCCGCTCGACGAAGGGGCACCGCCGCCCGCGATGCCCGACTGGTCGCCGGTGCGC
CGCTTCGGCGGCTACGGCGCTTGGCTCGACAAGGGGGAGGACGGCAAGGTGCGTGCCCAGGCGATGCAGATGTGCGGCTG
CGCCAATGCCTGCGGCGTGCACGGCCACGATCACGCGCGCTCCTGGTCGAGCCAGCTGCCGGTGTCCGATCTCAAGAGCT
TTTGGGGCGCGCTCGGCTGCGCCTGCTGGGCCGTGTGA

Upstream 100 bases:

>100_bases
GGCAGAAGGCGGCCTGCGCCACGCACATCTTCGGCCTGCTGCCCGGCCATCACGGCCGGGCAGAGACCACGAACTCTCCT
GCGCCAGACGAGAAGGCCTC

Downstream 100 bases:

>100_bases
TGGCGGCGAACGCGGACACGCCGCGGCCGATCGCAGCGGTGCTTGCCTGGCCATGGATATGGCCGCTGGCCCGGCTCGCA
TTGGTCTCGGCCTATCTGAT

Product: putative metallo-dependent hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSDHPDLILHRGLFTTLDRSNPQADAVAIKDGLFTAVGHAQDVMKLAGPQTQVIDLRGRRVLPGLIDNHLHIIRGGLNFN
MELRWDGVRSLADAMAMLKAQVAITPPPQWVRVVGGFTEHQFAEKRLPTIDELNAVAPETPVFLLHLYDRAILNGAALRA
VGYDKDTPQPPGGEIVRDAQGNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNY
PDDYAVIQKLADDGQLTIRLAYNLFTQKPKGEKDDFLRWTQSSAYKQGNDYFRHNGAGEMLVFSAADFEDFRVPRPDMPA
EMEGELEQVVRILVQNKWPWRLHATYDETISRALDVFEKVNQDMPLEGLHWFFDHAETISDQSIDRIAALGGGIAVQHRM
AYQGEYFVERYGCGAAEATPPVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGLRLYPQRNCLDRETALRMW
TENVTWFSNEEGRKGRIAVGQLADLIVPDRDYFACAEVEIADITSHLTMVGGRIVYAAADFKPLDEGAPPPAMPDWSPVR
RFGGYGAWLDKGEDGKVRAQAMQMCGCANACGVHGHDHARSWSSQLPVSDLKSFWGALGCACWAV

Sequences:

>Translated_625_residues
MSDHPDLILHRGLFTTLDRSNPQADAVAIKDGLFTAVGHAQDVMKLAGPQTQVIDLRGRRVLPGLIDNHLHIIRGGLNFN
MELRWDGVRSLADAMAMLKAQVAITPPPQWVRVVGGFTEHQFAEKRLPTIDELNAVAPETPVFLLHLYDRAILNGAALRA
VGYDKDTPQPPGGEIVRDAQGNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNY
PDDYAVIQKLADDGQLTIRLAYNLFTQKPKGEKDDFLRWTQSSAYKQGNDYFRHNGAGEMLVFSAADFEDFRVPRPDMPA
EMEGELEQVVRILVQNKWPWRLHATYDETISRALDVFEKVNQDMPLEGLHWFFDHAETISDQSIDRIAALGGGIAVQHRM
AYQGEYFVERYGCGAAEATPPVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGLRLYPQRNCLDRETALRMW
TENVTWFSNEEGRKGRIAVGQLADLIVPDRDYFACAEVEIADITSHLTMVGGRIVYAAADFKPLDEGAPPPAMPDWSPVR
RFGGYGAWLDKGEDGKVRAQAMQMCGCANACGVHGHDHARSWSSQLPVSDLKSFWGALGCACWAV
>Mature_624_residues
SDHPDLILHRGLFTTLDRSNPQADAVAIKDGLFTAVGHAQDVMKLAGPQTQVIDLRGRRVLPGLIDNHLHIIRGGLNFNM
ELRWDGVRSLADAMAMLKAQVAITPPPQWVRVVGGFTEHQFAEKRLPTIDELNAVAPETPVFLLHLYDRAILNGAALRAV
GYDKDTPQPPGGEIVRDAQGNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNYP
DDYAVIQKLADDGQLTIRLAYNLFTQKPKGEKDDFLRWTQSSAYKQGNDYFRHNGAGEMLVFSAADFEDFRVPRPDMPAE
MEGELEQVVRILVQNKWPWRLHATYDETISRALDVFEKVNQDMPLEGLHWFFDHAETISDQSIDRIAALGGGIAVQHRMA
YQGEYFVERYGCGAAEATPPVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGLRLYPQRNCLDRETALRMWT
ENVTWFSNEEGRKGRIAVGQLADLIVPDRDYFACAEVEIADITSHLTMVGGRIVYAAADFKPLDEGAPPPAMPDWSPVRR
FGGYGAWLDKGEDGKVRAQAMQMCGCANACGVHGHDHARSWSSQLPVSDLKSFWGALGCACWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 69152; Mature: 69021

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHPDLILHRGLFTTLDRSNPQADAVAIKDGLFTAVGHAQDVMKLAGPQTQVIDLRGRR
CCCCCCEEECCCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCE
VLPGLIDNHLHIIRGGLNFNMELRWDGVRSLADAMAMLKAQVAITPPPQWVRVVGGFTEH
ECCHHHCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCHHH
QFAEKRLPTIDELNAVAPETPVFLLHLYDRAILNGAALRAVGYDKDTPQPPGGEIVRDAQ
HHHHHCCCCHHHHCCCCCCCCEEEEEHHHHHHHCCHHEEEECCCCCCCCCCCCCEEECCC
GNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNY
CCCCEEEEECCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCC
PDDYAVIQKLADDGQLTIRLAYNLFTQKPKGEKDDFLRWTQSSAYKQGNDYFRHNGAGEM
CHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCE
LVFSAADFEDFRVPRPDMPAEMEGELEQVVRILVQNKWPWRLHATYDETISRALDVFEKV
EEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
NQDMPLEGLHWFFDHAETISDQSIDRIAALGGGIAVQHRMAYQGEYFVERYGCGAAEATP
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEHHHHHCCCHHHHHHCCCCCCCCC
PVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGLRLYPQRNCLDRETALRMW
HHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCEECCEEECCCCCCCCHHHHHHHH
TENVTWFSNEEGRKGRIAVGQLADLIVPDRDYFACAEVEIADITSHLTMVGGRIVYAAAD
HCCCEEECCCCCCCCCEEHHHHHHHHCCCCCCEEEECEEHHHHHHHHHHHCCEEEEEECC
FKPLDEGAPPPAMPDWSPVRRFGGYGAWLDKGEDGKVRAQAMQMCGCANACGVHGHDHAR
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHH
SWSSQLPVSDLKSFWGALGCACWAV
HHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDHPDLILHRGLFTTLDRSNPQADAVAIKDGLFTAVGHAQDVMKLAGPQTQVIDLRGRR
CCCCCEEECCCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCE
VLPGLIDNHLHIIRGGLNFNMELRWDGVRSLADAMAMLKAQVAITPPPQWVRVVGGFTEH
ECCHHHCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCHHH
QFAEKRLPTIDELNAVAPETPVFLLHLYDRAILNGAALRAVGYDKDTPQPPGGEIVRDAQ
HHHHHCCCCHHHHCCCCCCCCEEEEEHHHHHHHCCHHEEEECCCCCCCCCCCCCEEECCC
GNPTGLLLAKPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNY
CCCCEEEEECCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCC
PDDYAVIQKLADDGQLTIRLAYNLFTQKPKGEKDDFLRWTQSSAYKQGNDYFRHNGAGEM
CHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCE
LVFSAADFEDFRVPRPDMPAEMEGELEQVVRILVQNKWPWRLHATYDETISRALDVFEKV
EEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
NQDMPLEGLHWFFDHAETISDQSIDRIAALGGGIAVQHRMAYQGEYFVERYGCGAAEATP
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEHHHHHCCCHHHHHHCCCCCCCCC
PVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGLRLYPQRNCLDRETALRMW
HHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCEECCEEECCCCCCCCHHHHHHHH
TENVTWFSNEEGRKGRIAVGQLADLIVPDRDYFACAEVEIADITSHLTMVGGRIVYAAAD
HCCCEEECCCCCCCCCEEHHHHHHHHCCCCCCEEEECEEHHHHHHHHHHHCCEEEEEECC
FKPLDEGAPPPAMPDWSPVRRFGGYGAWLDKGEDGKVRAQAMQMCGCANACGVHGHDHAR
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHH
SWSSQLPVSDLKSFWGALGCACWAV
HHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA