Click here to switch to the map view.

The map label for this gene is fumA [H]

Identifier: 146341883

GI number: 146341883

Start: 5205261

End: 5206916

Strand: Reverse

Name: fumA [H]

Synonym: BRADO5016

Alternate gene names: 146341883

Gene position: 5206916-5205261 (Counterclockwise)

Preceding gene: 146341886

Following gene: 146341882

Centisome position: 69.83

GC content: 65.76

Gene sequence:

>1656_bases
ATGAACGCGCCCACCGCTTTCCCCGACCAGTCCAAGCCCGTCCCGCCCTACAAGCACACGCCGCTGTTTCCGCTCGGGCC
GGACACCACGCCCTACAAGAAGATCACGACCGAGGGCATCAGGGTCGAGAAGGTGCTGGGCCGCGACATGCTGGTCGTCT
CGCGCGAGGCGCTGCGGACCCTGTCGGAGGCCGCGTTCGGCGAGATCAACCACTTCCTGCGCCCTAGCCACCTGAAGCAG
CTGCGCGGCATCCTCGAGGATCCCGAGGCCAGCCCGAACGACAAGTTCGTCGCGCTCGACTTTCTCAAGAACGCCAACAT
CTCCGCCGGCGGTGTGCTGCCGATGTGCCAGGATACCGGCACCGCGATCATCATGGGCAAGAAGGGCTGCAACGTCATCA
CCGACGGTGACGACGAGGCCGCGCTCTCGGAAGGCGCGCGCGACGCTTACTTGCGCCGGAACCTGCGCTACTCGCAGGTC
GCGCCGCTGTCGATGTACGAGGAGAAGAACACCGCCAACAACATGCCGGCGCAGACCGAGATCTACGCCGAGGGTGAGGA
CGCCTACAAATTCATGTTCATGGCCAAGGGCGGCGGCTCGGCCAACAAGAGCTTCCTGTTCCAGGCGACGCCCTCGGTGC
TGACCAAGGACCGGCTGCTGGCGTTCCTGAAGGAGAAGATCCTGACGCTCGGCACCGCCGCGTGCCCGCCTTATCACCTC
GCCATCGTCATCGGCGGCACCTCGGCCGAGCTGTGCATGAAGACGGTGAAGTTGGCCTCGGCGCGCTACCTCGATGCGCT
GCCGACCCACGGCTCCGCCGACGGCAACGCCTTCCGCGACGTCGAGATGGAGCAGGAAATCCACAAGATGACGCAGTCGC
TCGGCGTCGGCGCGCAGTTCGGCGGCAAGTACTTCTGTCATGACGTGCGCGTCATCCGCATGCCGCGCCATGGCGCAAGC
TTGCCGATCGGCTTGGGCGTGTCGTGCTCGGCCGACCGCCAGGTGCTCGGCAAGATCACCAAGGACGGCGTCTATCTCGA
GGAGCTCGAGCATCACCCGGCGCAGTATCTGCCGGAGGTCGAGCAGGCGCTCGGCGGCGAGGTCGTGAAGATCGACCTGA
ACCAGCCAATGCAGGACATCCTCGCCACGCTGTCGAAGAATCCGATCAAGACCCGGCTGTCGCTGACCGGCACCATGATC
GTCGCCCGCGACGCCGCGCATGCCAAGCTGCGCGAGCGCCTCGACCGCGGCGAGCCGCTGCCGGACTACTTCAAGAACCA
TCCGGTGTACTACGCCGGTCCGGCCAAGACGCCCGAGGGCTACGCCTCCGGCGCGTTCGGTCCGACCACCGCTGGCCGCA
TGGATTCCTTCGTCGACCAGTTCCAGGCCGCCGGCGGCTCCATGGTCATGGTCGCCAAGGGCAACCGCGCGCCGGCCGTG
CGCGAAGCCTGCAAGACGCACGGCGGCTTCTATCTCGGCTCGATCGGCGGCGCGGCGGCGAACCTTGCCGAGCACTGCAT
CAAGAAGGTCGAGGTCGTCGAATATCCCGAACTCGGCATGGAAGCGATCTGGCGCATCGAGGTCGTGGACTTCCCGGCCT
TCATCATCATCGACGACAAGGGCAACGACTTCTTCAAGGAGCTGAACCTCGGCTGA

Upstream 100 bases:

>100_bases
GCCATGCCGAACCATATGTTGCGCGTCGGCCCCAGGGTGATATCAGACCTGTCAAGACCGCTTTGGGCGGCCGCCTTTAG
AACCCCTGCGAGATTTCGCC

Downstream 100 bases:

>100_bases
CCGCTCACCGACATAATCCACTATGGATGTGAGGGCGGCATGATTGAGTGGTTCGACGACCTCAAGATCGGGATGACGTT
CAAGAGCGGCGAGACGACAG

Product: fumarase A

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MNAPTAFPDQSKPVPPYKHTPLFPLGPDTTPYKKITTEGIRVEKVLGRDMLVVSREALRTLSEAAFGEINHFLRPSHLKQ
LRGILEDPEASPNDKFVALDFLKNANISAGGVLPMCQDTGTAIIMGKKGCNVITDGDDEAALSEGARDAYLRRNLRYSQV
APLSMYEEKNTANNMPAQTEIYAEGEDAYKFMFMAKGGGSANKSFLFQATPSVLTKDRLLAFLKEKILTLGTAACPPYHL
AIVIGGTSAELCMKTVKLASARYLDALPTHGSADGNAFRDVEMEQEIHKMTQSLGVGAQFGGKYFCHDVRVIRMPRHGAS
LPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEVEQALGGEVVKIDLNQPMQDILATLSKNPIKTRLSLTGTMI
VARDAAHAKLRERLDRGEPLPDYFKNHPVYYAGPAKTPEGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAPAV
REACKTHGGFYLGSIGGAAANLAEHCIKKVEVVEYPELGMEAIWRIEVVDFPAFIIIDDKGNDFFKELNLG

Sequences:

>Translated_551_residues
MNAPTAFPDQSKPVPPYKHTPLFPLGPDTTPYKKITTEGIRVEKVLGRDMLVVSREALRTLSEAAFGEINHFLRPSHLKQ
LRGILEDPEASPNDKFVALDFLKNANISAGGVLPMCQDTGTAIIMGKKGCNVITDGDDEAALSEGARDAYLRRNLRYSQV
APLSMYEEKNTANNMPAQTEIYAEGEDAYKFMFMAKGGGSANKSFLFQATPSVLTKDRLLAFLKEKILTLGTAACPPYHL
AIVIGGTSAELCMKTVKLASARYLDALPTHGSADGNAFRDVEMEQEIHKMTQSLGVGAQFGGKYFCHDVRVIRMPRHGAS
LPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEVEQALGGEVVKIDLNQPMQDILATLSKNPIKTRLSLTGTMI
VARDAAHAKLRERLDRGEPLPDYFKNHPVYYAGPAKTPEGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAPAV
REACKTHGGFYLGSIGGAAANLAEHCIKKVEVVEYPELGMEAIWRIEVVDFPAFIIIDDKGNDFFKELNLG
>Mature_551_residues
MNAPTAFPDQSKPVPPYKHTPLFPLGPDTTPYKKITTEGIRVEKVLGRDMLVVSREALRTLSEAAFGEINHFLRPSHLKQ
LRGILEDPEASPNDKFVALDFLKNANISAGGVLPMCQDTGTAIIMGKKGCNVITDGDDEAALSEGARDAYLRRNLRYSQV
APLSMYEEKNTANNMPAQTEIYAEGEDAYKFMFMAKGGGSANKSFLFQATPSVLTKDRLLAFLKEKILTLGTAACPPYHL
AIVIGGTSAELCMKTVKLASARYLDALPTHGSADGNAFRDVEMEQEIHKMTQSLGVGAQFGGKYFCHDVRVIRMPRHGAS
LPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEVEQALGGEVVKIDLNQPMQDILATLSKNPIKTRLSLTGTMI
VARDAAHAKLRERLDRGEPLPDYFKNHPVYYAGPAKTPEGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAPAV
REACKTHGGFYLGSIGGAAANLAEHCIKKVEVVEYPELGMEAIWRIEVVDFPAFIIIDDKGNDFFKELNLG

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=536, Percent_Identity=62.1268656716418, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=534, Percent_Identity=61.2359550561798, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59941; Mature: 59941

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTAFPDQSKPVPPYKHTPLFPLGPDTTPYKKITTEGIRVEKVLGRDMLVVSREALRT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEHHHHCCCHHHHHHHHHHH
LSEAAFGEINHFLRPSHLKQLRGILEDPEASPNDKFVALDFLKNANISAGGVLPMCQDTG
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCC
TAIIMGKKGCNVITDGDDEAALSEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQTE
CEEEEECCCCCEEECCCCHHHHHCCHHHHHHHHCCCHHHCCCHHHHHCCCCCCCCCCCEE
IYAEGEDAYKFMFMAKGGGSANKSFLFQATPSVLTKDRLLAFLKEKILTLGTAACPPYHL
EEECCCCEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
AIVIGGTSAELCMKTVKLASARYLDALPTHGSADGNAFRDVEMEQEIHKMTQSLGVGAQF
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
GGKYFCHDVRVIRMPRHGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEV
CCEEEEECEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEHHHHHCCCHHHHHHH
EQALGGEVVKIDLNQPMQDILATLSKNPIKTRLSLTGTMIVARDAAHAKLRERLDRGEPL
HHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHCCCCC
PDYFKNHPVYYAGPAKTPEGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAPAV
HHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
REACKTHGGFYLGSIGGAAANLAEHCIKKVEVVEYPELGMEAIWRIEVVDFPAFIIIDDK
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCEEEEEECC
GNDFFKELNLG
CCHHHHHCCCC
>Mature Secondary Structure
MNAPTAFPDQSKPVPPYKHTPLFPLGPDTTPYKKITTEGIRVEKVLGRDMLVVSREALRT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEHHHHCCCHHHHHHHHHHH
LSEAAFGEINHFLRPSHLKQLRGILEDPEASPNDKFVALDFLKNANISAGGVLPMCQDTG
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCC
TAIIMGKKGCNVITDGDDEAALSEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQTE
CEEEEECCCCCEEECCCCHHHHHCCHHHHHHHHCCCHHHCCCHHHHHCCCCCCCCCCCEE
IYAEGEDAYKFMFMAKGGGSANKSFLFQATPSVLTKDRLLAFLKEKILTLGTAACPPYHL
EEECCCCEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
AIVIGGTSAELCMKTVKLASARYLDALPTHGSADGNAFRDVEMEQEIHKMTQSLGVGAQF
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
GGKYFCHDVRVIRMPRHGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEV
CCEEEEECEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEHHHHHCCCHHHHHHH
EQALGGEVVKIDLNQPMQDILATLSKNPIKTRLSLTGTMIVARDAAHAKLRERLDRGEPL
HHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHCCCCC
PDYFKNHPVYYAGPAKTPEGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAPAV
HHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
REACKTHGGFYLGSIGGAAANLAEHCIKKVEVVEYPELGMEAIWRIEVVDFPAFIIIDDK
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCEEEEEECC
GNDFFKELNLG
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]