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The map label for this gene is gcvH [H]

Identifier: 146341853

GI number: 146341853

Start: 5180008

End: 5180373

Strand: Direct

Name: gcvH [H]

Synonym: BRADO4983

Alternate gene names: 146341853

Gene position: 5180008-5180373 (Clockwise)

Preceding gene: 146341852

Following gene: 146341854

Centisome position: 69.47

GC content: 65.03

Gene sequence:

>366_bases
ATGACCACGCTGTACACCTCCGACCATGAATGGCTCAGCATCGAGGGCGATGTCGCCACCATCGGCATCACGGACTATGC
GCAGGCGCAGCTCGGCGATGTCGTGTTCGTCGAGCTGCCGCAGGTCGGCCGTGCGCTGAAGAAGACGGAAGCCGCCGCCG
TGGTCGAATCGGTCAAGGCCGCCTCCGACGTCTACGCGCCGATCACCGGCGAGGTGCTCGAGGTCAACCAGGCCATCGTC
GACGAGCCGGCGCTGGTCAACACCGATGCCGACGGCAAGGCCTGGTTCTTTAAGCTCAAGATCGCCGACAAGAGCGAGCT
CGGCGGCCTGATGGACGAGGCCGCCTACAAGGCACACACGGCGTGA

Upstream 100 bases:

>100_bases
GGACACCCAATTGTTCGCCGATGTGCGCGGGCAACGCCTGCCGCTGCGCGTCGCCGCCACGCCTTTTGTCCCCAACACCT
ACAAGCGCTGAAGGACGTCC

Downstream 100 bases:

>100_bases
TGCTCCCGCGGGTCACCACACCCGCCGTCATGCCCCGCGCAGGCGGGGCATCCAGTACTCCCGGCGGCAGCGGTCGACCA
GAGACGCCTCGGCGTACTGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 120

Protein sequence:

>121_residues
MTTLYTSDHEWLSIEGDVATIGITDYAQAQLGDVVFVELPQVGRALKKTEAAAVVESVKAASDVYAPITGEVLEVNQAIV
DEPALVNTDADGKAWFFKLKIADKSELGGLMDEAAYKAHTA

Sequences:

>Translated_121_residues
MTTLYTSDHEWLSIEGDVATIGITDYAQAQLGDVVFVELPQVGRALKKTEAAAVVESVKAASDVYAPITGEVLEVNQAIV
DEPALVNTDADGKAWFFKLKIADKSELGGLMDEAAYKAHTA
>Mature_120_residues
TTLYTSDHEWLSIEGDVATIGITDYAQAQLGDVVFVELPQVGRALKKTEAAAVVESVKAASDVYAPITGEVLEVNQAIVD
EPALVNTDADGKAWFFKLKIADKSELGGLMDEAAYKAHTA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=43.4782608695652, Blast_Score=117, Evalue=2e-27,
Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=43.4782608695652, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789271, Length=115, Percent_Identity=51.304347826087, Blast_Score=122, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=46.9026548672566, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=48.6725663716814, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=117, Percent_Identity=40.1709401709402, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17865652, Length=115, Percent_Identity=47.8260869565217, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12929; Mature: 12797

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLYTSDHEWLSIEGDVATIGITDYAQAQLGDVVFVELPQVGRALKKTEAAAVVESVKA
CCEEECCCCCEEEEECCEEEEECCCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ASDVYAPITGEVLEVNQAIVDEPALVNTDADGKAWFFKLKIADKSELGGLMDEAAYKAHT
HHHCCCCCCCCEEECCHHHCCCCCEEECCCCCCEEEEEEEECCCHHHCCHHHHHHHHCCC
A
C
>Mature Secondary Structure 
TTLYTSDHEWLSIEGDVATIGITDYAQAQLGDVVFVELPQVGRALKKTEAAAVVESVKA
CEEECCCCCEEEEECCEEEEECCCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ASDVYAPITGEVLEVNQAIVDEPALVNTDADGKAWFFKLKIADKSELGGLMDEAAYKAHT
HHHCCCCCCCCEEECCHHHCCCCCEEECCCCCCEEEEEEEECCCHHHCCHHHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]