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The map label for this gene is gcl [H]

Identifier: 146341819

GI number: 146341819

Start: 5136906

End: 5138693

Strand: Reverse

Name: gcl [H]

Synonym: BRADO4946

Alternate gene names: 146341819

Gene position: 5138693-5136906 (Counterclockwise)

Preceding gene: 146341820

Following gene: 146341818

Centisome position: 68.91

GC content: 67.0

Gene sequence:

>1788_bases
ATGGCGAAGATGCGGGCGATCGATGCTGCGGTGCGGATCTTGGAGAAGGAGGGCGTCGCCTGCGCCTTCGGCGTTCCCGG
CGCGGCGATCAATCCGCTCTATTCCGCGCTGCAACGGCGCGGCTCGATCCGCCACATTCTCGCCCGCCACGTCGAGGGCG
CCTCGCACATGGCCGAGGGCTACACCCGCGCCAAGGCCGGCAATATCGGCGTGTGCATCGGCACGTCGGGGCCGGCGGGC
ACCGACATGATCACCGGACTTTACTCGGCGATCGCGGATTCGATTCCGATCCTCTGCATCACCGGCCAGGCGCCGCGGGC
GCGGCTCTACAAGGAGGATTTCCAGGCCGTCGACATCGAGCAGATCGCCAAGCCCGTGACCAAATGGGCGGTGACGGTGC
GCGAGCCCGGCCTGGTGCCGCGGGTGTTCAGCCAGGCCTTCCACATCATGCGCTCGGGACGCCCGGGTCCTGTCCTGATC
GACCTGCCGCTCGACGTGCAGCTCGCCGAGATCGAGTTCGACGACGAGACCTATGCGCCGCTGCCGGTCTACAAGCCCGC
CGCGACCCGCAAGCAGATCGAGAAGGCGCTGGAGATGCTGAACGCCGCCGAGCGTCCGCTGATCGTCGCCGGCGGCGGCA
TCATCAACGCCGATGCCAGCGATCTCCTCGTCACGTTCGCCGAGACCGTCAACGTGCCCGTCGTGCCGACCCTGATGGGG
TGGGGCGCCATTCCCGACGATTATTTGCTGATGGCCGGCATGGTCGGGCTGCAGACCAGCCACCGCTACGGCAACGCCAA
TTTCCTGGAGTCCGATTTCGTCCTCGGCATCGGCAATCGCTGGGCCAACAGACATACGGGCTCGCTCGAGACCTACACCA
AGGGCCGCACCTTCGTGCATGTCGATATCGAGCCGACCCAGATCGGCCGCGTGTTCAATCCGGAGCTCGGCATCGTCTCC
GATGCCAAGGCCGCGCTGGAGATGTTCATCGCCGTCGCGCGCGAGTGGCGCAAGGCGGGGAAGCTGCGTGAGCGGCAGGC
ATGGCCCGCGGCGTGCCAGGACCGCAAGCGCACCATGCTCCGGCGCAGCGATTTTGACCAGGTGCCGATCAAGCCGCAGC
GCGTCTATCACGAGATGAACAAGGCGTTCGGCCGCGACACCTGCTATGTCAGCGTCATCGGTCTGTCGCAGATCGGCGGC
GCGCAGTTCCTCAGCGTCAACCACCCGCGGCATTGGATCAATGCCGGGCAGGCGGGCCCGCTCGGCTGGACCCTGCCGGC
CGCGCTCGGCGTCCGCGCCGCCGATCCCAAGCGCGAGATCGTCGCGCTGTCCGGCGACTACGACTTCCAGTTCCTGATCG
AGGAGCTCGCGGTCGGCGCCCAGTTCAATCTGCCTTACATCCACGTCGTCGTGAACAATGCCTATCTCGGGCTGATCCGG
CAGGCGCAGCGCGGCTTCTCGATGGACTACCAGGTGCAGCTGTCGTTCGAGAACATCAACGCGCCGGAGGTCGGCGGCTA
CGGCGTCGACCATGTCGCGGTGGCGCAAGGCCTCGGCTGCAAGGCGATCCGCGTCACCGATCCGCGCCAGGCGCAGGCGG
CGTTCGCGACCGCGCGCGAATGGATGGCGGAGTATCAGGTGCCCGTGGTGGTCGAGTTCGTACTCGAGCGGGTCACCAAC
ATCGCGATGGGTACCGAGATCGACAATGTCGTGGAGTTCGAGGAGGTGCTCGACCTGCCGGTGGACGAGACGCCGGCCGA
GTCGCAGCGCACCCTGCAGCCGGCGTGA

Upstream 100 bases:

>100_bases
GTTTTGCCGAGCATGCCGCATCGTGGGAAAAATCGACGACTGAATTGAGATCGCTGCCCACAGCAGCGATCCTCGCGCAT
CAAAAAGATGGAGGCGCGAG

Downstream 100 bases:

>100_bases
TCGCAGCCAACGCATTCAGTCGAGGACCACCCTATGCCGCACTTTGCCGCCAACCTGACGATGCTGTTCAACGAGCTGCC
GTTTCTGGAGCGTTTCGCCG

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MAKMRAIDAAVRILEKEGVACAFGVPGAAINPLYSALQRRGSIRHILARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAG
TDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIEQIAKPVTKWAVTVREPGLVPRVFSQAFHIMRSGRPGPVLI
DLPLDVQLAEIEFDDETYAPLPVYKPAATRKQIEKALEMLNAAERPLIVAGGGIINADASDLLVTFAETVNVPVVPTLMG
WGAIPDDYLLMAGMVGLQTSHRYGNANFLESDFVLGIGNRWANRHTGSLETYTKGRTFVHVDIEPTQIGRVFNPELGIVS
DAKAALEMFIAVAREWRKAGKLRERQAWPAACQDRKRTMLRRSDFDQVPIKPQRVYHEMNKAFGRDTCYVSVIGLSQIGG
AQFLSVNHPRHWINAGQAGPLGWTLPAALGVRAADPKREIVALSGDYDFQFLIEELAVGAQFNLPYIHVVVNNAYLGLIR
QAQRGFSMDYQVQLSFENINAPEVGGYGVDHVAVAQGLGCKAIRVTDPRQAQAAFATAREWMAEYQVPVVVEFVLERVTN
IAMGTEIDNVVEFEEVLDLPVDETPAESQRTLQPA

Sequences:

>Translated_595_residues
MAKMRAIDAAVRILEKEGVACAFGVPGAAINPLYSALQRRGSIRHILARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAG
TDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIEQIAKPVTKWAVTVREPGLVPRVFSQAFHIMRSGRPGPVLI
DLPLDVQLAEIEFDDETYAPLPVYKPAATRKQIEKALEMLNAAERPLIVAGGGIINADASDLLVTFAETVNVPVVPTLMG
WGAIPDDYLLMAGMVGLQTSHRYGNANFLESDFVLGIGNRWANRHTGSLETYTKGRTFVHVDIEPTQIGRVFNPELGIVS
DAKAALEMFIAVAREWRKAGKLRERQAWPAACQDRKRTMLRRSDFDQVPIKPQRVYHEMNKAFGRDTCYVSVIGLSQIGG
AQFLSVNHPRHWINAGQAGPLGWTLPAALGVRAADPKREIVALSGDYDFQFLIEELAVGAQFNLPYIHVVVNNAYLGLIR
QAQRGFSMDYQVQLSFENINAPEVGGYGVDHVAVAQGLGCKAIRVTDPRQAQAAFATAREWMAEYQVPVVVEFVLERVTN
IAMGTEIDNVVEFEEVLDLPVDETPAESQRTLQPA
>Mature_594_residues
AKMRAIDAAVRILEKEGVACAFGVPGAAINPLYSALQRRGSIRHILARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAGT
DMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIEQIAKPVTKWAVTVREPGLVPRVFSQAFHIMRSGRPGPVLID
LPLDVQLAEIEFDDETYAPLPVYKPAATRKQIEKALEMLNAAERPLIVAGGGIINADASDLLVTFAETVNVPVVPTLMGW
GAIPDDYLLMAGMVGLQTSHRYGNANFLESDFVLGIGNRWANRHTGSLETYTKGRTFVHVDIEPTQIGRVFNPELGIVSD
AKAALEMFIAVAREWRKAGKLRERQAWPAACQDRKRTMLRRSDFDQVPIKPQRVYHEMNKAFGRDTCYVSVIGLSQIGGA
QFLSVNHPRHWINAGQAGPLGWTLPAALGVRAADPKREIVALSGDYDFQFLIEELAVGAQFNLPYIHVVVNNAYLGLIRQ
AQRGFSMDYQVQLSFENINAPEVGGYGVDHVAVAQGLGCKAIRVTDPRQAQAAFATAREWMAEYQVPVVVEFVLERVTNI
AMGTEIDNVVEFEEVLDLPVDETPAESQRTLQPA

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=23.3511586452763, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI21361361, Length=522, Percent_Identity=23.7547892720307, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786717, Length=588, Percent_Identity=74.6598639455782, Blast_Score=921, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=545, Percent_Identity=33.5779816513761, Blast_Score=308, Evalue=8e-85,
Organism=Escherichia coli, GI1790104, Length=516, Percent_Identity=32.9457364341085, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1787096, Length=474, Percent_Identity=25.9493670886076, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788716, Length=475, Percent_Identity=25.2631578947368, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=512, Percent_Identity=25.78125, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=512, Percent_Identity=25.78125, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=29.6296296296296, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=24.2798353909465, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=534, Percent_Identity=22.2846441947566, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=26.1101243339254, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 65108; Mature: 64977

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAIDAAVRILEKEGVACAFGVPGAAINPLYSALQRRGSIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
YTRAKAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCHH
QIAKPVTKWAVTVREPGLVPRVFSQAFHIMRSGRPGPVLIDLPLDVQLAEIEFDDETYAP
HHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAATRKQIEKALEMLNAAERPLIVAGGGIINADASDLLVTFAETVNVPVVPTLMG
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCCHHHHC
WGAIPDDYLLMAGMVGLQTSHRYGNANFLESDFVLGIGNRWANRHTGSLETYTKGRTFVH
CCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEECCCCCCCCCCCCCEEEECCCEEEE
VDIEPTQIGRVFNPELGIVSDAKAALEMFIAVAREWRKAGKLRERQAWPAACQDRKRTML
EECCHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RRSDFDQVPIKPQRVYHEMNKAFGRDTCYVSVIGLSQIGGAQFLSVNHPRHWINAGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHCCCEEEEEECCCHHHHCCCCCCC
LGWTLPAALGVRAADPKREIVALSGDYDFQFLIEELAVGAQFNLPYIHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QAQRGFSMDYQVQLSFENINAPEVGGYGVDHVAVAQGLGCKAIRVTDPRQAQAAFATARE
HHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
WMAEYQVPVVVEFVLERVTNIAMGTEIDNVVEFEEVLDLPVDETPAESQRTLQPA
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKMRAIDAAVRILEKEGVACAFGVPGAAINPLYSALQRRGSIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
YTRAKAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCHH
QIAKPVTKWAVTVREPGLVPRVFSQAFHIMRSGRPGPVLIDLPLDVQLAEIEFDDETYAP
HHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAATRKQIEKALEMLNAAERPLIVAGGGIINADASDLLVTFAETVNVPVVPTLMG
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCCHHHHC
WGAIPDDYLLMAGMVGLQTSHRYGNANFLESDFVLGIGNRWANRHTGSLETYTKGRTFVH
CCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEECCCCCCCCCCCCCEEEECCCEEEE
VDIEPTQIGRVFNPELGIVSDAKAALEMFIAVAREWRKAGKLRERQAWPAACQDRKRTML
EECCHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RRSDFDQVPIKPQRVYHEMNKAFGRDTCYVSVIGLSQIGGAQFLSVNHPRHWINAGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHCCCEEEEEECCCHHHHCCCCCCC
LGWTLPAALGVRAADPKREIVALSGDYDFQFLIEELAVGAQFNLPYIHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QAQRGFSMDYQVQLSFENINAPEVGGYGVDHVAVAQGLGCKAIRVTDPRQAQAAFATARE
HHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
WMAEYQVPVVVEFVLERVTNIAMGTEIDNVVEFEEVLDLPVDETPAESQRTLQPA
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]