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The map label for this gene is ramA [H]

Identifier: 146341737

GI number: 146341737

Start: 5055920

End: 5057659

Strand: Reverse

Name: ramA [H]

Synonym: BRADO4852

Alternate gene names: 146341737

Gene position: 5057659-5055920 (Counterclockwise)

Preceding gene: 146341738

Following gene: 146341736

Centisome position: 67.83

GC content: 65.63

Gene sequence:

>1740_bases
ATGCCGATGACGTACAAGGCCGCGACCGTCCAGTTCGAGCCTGTTCTGGCCGGGAAGGAGCGCAACATCGAAGGCCTGCT
CGCGCTGTGCGAGCAGGCGGCCGCGTCGGGCGCCAAGCTGATCGTGACGCCGGAGATGGGCACGACAGGCTATTGCTGGT
ACGACCGCGCGGAGGTGGCGCCCTATGTCGAGAAGGTGCCGGGCTCGACCACGCACCGCTTCGCATCGCTGGCGCGACGG
CATGATTGTTACATCGTGATCGGCATGCCCGAGGTGGATGACGACGACATCTACTACAACAGCGCGGTGCTGATCGGCCC
GGATGGCGTGATCGGCCGGCACCGCAAGACGCATCCGTATATCTCGGAGCCAAAATGGGCGGCGGCCGGCGACCTGCACA
ACCAGGTGTTCGAGACGCCGATCGGCCGCATCGCGCTCCTGATTTGCATGGACATCCATTTCGTCGAGACGGCGCGGCTG
ATGGCGCTCGGCGGCGCCGACATCATCTGCCACATCTCGAACTGGCTCGCCGAGCGCACGCCGGCGCCGTACTGGATCAG
CCGCGCTTTCGAGAACTCCTGCTACGTGATCGAGAGCAACCGCTGGGGGCTGGAGCGCACGGTGCAGTTCTCCGGCGGCA
GCTGCGTCATCGCGCCCGATGGCAGCCTGCCGGCCGTGATCGACAAGGGCGACGGCGTTGCCTTCGCAGAAATCGATCTT
GCATGGGCTCGGGCACGCAAGGTGCTGGGCGAGCCGGTGTTCGCACAGCGCCGGCCGGAGCTTTATCCTGAGCTGCTCAC
CAATACCTACAGCTGGAACCCGCGCGATTTCTTCGGGCTGTACGGCCACGAGCCGTGGCCGGTGGGCAAACGCTCCCGCG
TCAGCGTGGCGCAGTTTACGCCGAGCCCCGACGTCGCTTCCAATCTCGCCCGCATCGCCGAGCTCGCGGCTGCGGCGAAG
GAGAAGGGCGCCGAGCTGGTGGTATTCCCGGAACTTGCGGCGACGGGGCTGACCCATTCGGCCGAAACCGCCGAGCCGAT
CCCGGGCCGCATCACGGCTGCGCTCACCGAACTCGCCGCGGAGCGCGGGCTCACGCTGGTCTGCGGTCTTGCAGAACGAG
ACGGGAACACCATCTATAACTCAGCCGTCCTGGTCACGCCGGACGGTAAAATCAGCACTTACCGCAAGACGCATCTCACG
ACGGCCGAGCGCAGCTGGGCCACGGCGGGGGATGAGTGGACCGTTGTCGACACGCCGATGGGGCGGATCGGGATCCTGAT
CGGCCATGATGCGGTGTTTCCGGAGGCGGGCCGGGTGCTCGCCTTGCGCGGCTGCGATCTGATCGTGTGTCCCTCCGCGG
TGAAGGGCATCTTCAGCGCGGCGCATGCAGGGACGAAGGTGATGCAGCCGTCACCCATCCCGACCGGGGCCGATCCGTAT
CACTGGCATCATTTCCGCGTTCGCGGCGGCGAGAACAATGCCTACTTCGCGTTCGCGAATGTGTGCGACGCCGCGGATGA
GGATTGCGGGCTGAGCGGCGTGTTCGGTCCGGATACGTTTGCGTTTCCGCGCCGCGAGGCGATCGTCGATCGCGGGGAGG
GGATTGCGACGCTCGAGATCGACACCGGCAATCTCGACAGCGTCTATCCGACCAACGTCGTTCGCCGGAAAGATCTCGTC
TGCATGCGCATGCCGCACTCCTATCGAGCGCTGGTGCAATCCAAGGCGAGCAATTTCTGA

Upstream 100 bases:

>100_bases
TGACGCAGGCCGGGCGTGTCCGGCTCGGCAACATGGTCGATCCGAAATACACGCTCGGCGCCTCCATCAAGAAGCACTTC
CTGGTCTGAGGTCGCCGACA

Downstream 100 bases:

>100_bases
GCTTGCGCGGCCGCATCCGTTCCAAAATGGAACGGATGCGGCGTGTGACAGCGCATAGAGCAGGGTACGACAGCGCTATC
CACATTTCTTCACAGCCGTC

Product: putative nitrilase/cyanide hydratase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MPMTYKAATVQFEPVLAGKERNIEGLLALCEQAAASGAKLIVTPEMGTTGYCWYDRAEVAPYVEKVPGSTTHRFASLARR
HDCYIVIGMPEVDDDDIYYNSAVLIGPDGVIGRHRKTHPYISEPKWAAAGDLHNQVFETPIGRIALLICMDIHFVETARL
MALGGADIICHISNWLAERTPAPYWISRAFENSCYVIESNRWGLERTVQFSGGSCVIAPDGSLPAVIDKGDGVAFAEIDL
AWARARKVLGEPVFAQRRPELYPELLTNTYSWNPRDFFGLYGHEPWPVGKRSRVSVAQFTPSPDVASNLARIAELAAAAK
EKGAELVVFPELAATGLTHSAETAEPIPGRITAALTELAAERGLTLVCGLAERDGNTIYNSAVLVTPDGKISTYRKTHLT
TAERSWATAGDEWTVVDTPMGRIGILIGHDAVFPEAGRVLALRGCDLIVCPSAVKGIFSAAHAGTKVMQPSPIPTGADPY
HWHHFRVRGGENNAYFAFANVCDAADEDCGLSGVFGPDTFAFPRREAIVDRGEGIATLEIDTGNLDSVYPTNVVRRKDLV
CMRMPHSYRALVQSKASNF

Sequences:

>Translated_579_residues
MPMTYKAATVQFEPVLAGKERNIEGLLALCEQAAASGAKLIVTPEMGTTGYCWYDRAEVAPYVEKVPGSTTHRFASLARR
HDCYIVIGMPEVDDDDIYYNSAVLIGPDGVIGRHRKTHPYISEPKWAAAGDLHNQVFETPIGRIALLICMDIHFVETARL
MALGGADIICHISNWLAERTPAPYWISRAFENSCYVIESNRWGLERTVQFSGGSCVIAPDGSLPAVIDKGDGVAFAEIDL
AWARARKVLGEPVFAQRRPELYPELLTNTYSWNPRDFFGLYGHEPWPVGKRSRVSVAQFTPSPDVASNLARIAELAAAAK
EKGAELVVFPELAATGLTHSAETAEPIPGRITAALTELAAERGLTLVCGLAERDGNTIYNSAVLVTPDGKISTYRKTHLT
TAERSWATAGDEWTVVDTPMGRIGILIGHDAVFPEAGRVLALRGCDLIVCPSAVKGIFSAAHAGTKVMQPSPIPTGADPY
HWHHFRVRGGENNAYFAFANVCDAADEDCGLSGVFGPDTFAFPRREAIVDRGEGIATLEIDTGNLDSVYPTNVVRRKDLV
CMRMPHSYRALVQSKASNF
>Mature_578_residues
PMTYKAATVQFEPVLAGKERNIEGLLALCEQAAASGAKLIVTPEMGTTGYCWYDRAEVAPYVEKVPGSTTHRFASLARRH
DCYIVIGMPEVDDDDIYYNSAVLIGPDGVIGRHRKTHPYISEPKWAAAGDLHNQVFETPIGRIALLICMDIHFVETARLM
ALGGADIICHISNWLAERTPAPYWISRAFENSCYVIESNRWGLERTVQFSGGSCVIAPDGSLPAVIDKGDGVAFAEIDLA
WARARKVLGEPVFAQRRPELYPELLTNTYSWNPRDFFGLYGHEPWPVGKRSRVSVAQFTPSPDVASNLARIAELAAAAKE
KGAELVVFPELAATGLTHSAETAEPIPGRITAALTELAAERGLTLVCGLAERDGNTIYNSAVLVTPDGKISTYRKTHLTT
AERSWATAGDEWTVVDTPMGRIGILIGHDAVFPEAGRVLALRGCDLIVCPSAVKGIFSAAHAGTKVMQPSPIPTGADPYH
WHHFRVRGGENNAYFAFANVCDAADEDCGLSGVFGPDTFAFPRREAIVDRGEGIATLEIDTGNLDSVYPTNVVRRKDLVC
MRMPHSYRALVQSKASNF

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=291, Percent_Identity=29.553264604811, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI297632348, Length=267, Percent_Identity=29.5880149812734, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI297632350, Length=267, Percent_Identity=29.5880149812734, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI5031947, Length=267, Percent_Identity=29.5880149812734, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI297632346, Length=189, Percent_Identity=34.9206349206349, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI7706509, Length=243, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17533173, Length=251, Percent_Identity=33.0677290836653, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556280, Length=261, Percent_Identity=26.0536398467433, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557111, Length=294, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323383, Length=300, Percent_Identity=27.6666666666667, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322522, Length=130, Percent_Identity=30, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21358471, Length=283, Percent_Identity=28.6219081272085, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355835, Length=263, Percent_Identity=27.7566539923954, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17933642, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 63034; Mature: 62903

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTYKAATVQFEPVLAGKERNIEGLLALCEQAAASGAKLIVTPEMGTTGYCWYDRAEVA
CCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHC
PYVEKVPGSTTHRFASLARRHDCYIVIGMPEVDDDDIYYNSAVLIGPDGVIGRHRKTHPY
HHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCEEEECCCCCCCCCCCCCCC
ISEPKWAAAGDLHNQVFETPIGRIALLICMDIHFVETARLMALGGADIICHISNWLAERT
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEHHHHHHCC
PAPYWISRAFENSCYVIESNRWGLERTVQFSGGSCVIAPDGSLPAVIDKGDGVAFAEIDL
CCCHHHHHHCCCCEEEEECCCCCCEEEEEECCCEEEEECCCCCCEEEECCCCEEEEEEHH
AWARARKVLGEPVFAQRRPELYPELLTNTYSWNPRDFFGLYGHEPWPVGKRSRVSVAQFT
HHHHHHHHHCCCHHHHCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEC
PSPDVASNLARIAELAAAAKEKGAELVVFPELAATGLTHSAETAEPIPGRITAALTELAA
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
ERGLTLVCGLAERDGNTIYNSAVLVTPDGKISTYRKTHLTTAERSWATAGDEWTVVDTPM
HCCCEEEEEECCCCCCEEECCEEEECCCCCCEEEEHEECCCCCCCCCCCCCCEEEEECCC
GRIGILIGHDAVFPEAGRVLALRGCDLIVCPSAVKGIFSAAHAGTKVMQPSPIPTGADPY
CEEEEEEECCCCCCCCCCEEEEECCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HWHHFRVRGGENNAYFAFANVCDAADEDCGLSGVFGPDTFAFPRREAIVDRGEGIATLEI
EEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEE
DTGNLDSVYPTNVVRRKDLVCMRMPHSYRALVQSKASNF
CCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PMTYKAATVQFEPVLAGKERNIEGLLALCEQAAASGAKLIVTPEMGTTGYCWYDRAEVA
CCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHC
PYVEKVPGSTTHRFASLARRHDCYIVIGMPEVDDDDIYYNSAVLIGPDGVIGRHRKTHPY
HHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCEEEECCCCCCCCCCCCCCC
ISEPKWAAAGDLHNQVFETPIGRIALLICMDIHFVETARLMALGGADIICHISNWLAERT
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEHHHHHHCC
PAPYWISRAFENSCYVIESNRWGLERTVQFSGGSCVIAPDGSLPAVIDKGDGVAFAEIDL
CCCHHHHHHCCCCEEEEECCCCCCEEEEEECCCEEEEECCCCCCEEEECCCCEEEEEEHH
AWARARKVLGEPVFAQRRPELYPELLTNTYSWNPRDFFGLYGHEPWPVGKRSRVSVAQFT
HHHHHHHHHCCCHHHHCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEC
PSPDVASNLARIAELAAAAKEKGAELVVFPELAATGLTHSAETAEPIPGRITAALTELAA
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
ERGLTLVCGLAERDGNTIYNSAVLVTPDGKISTYRKTHLTTAERSWATAGDEWTVVDTPM
HCCCEEEEEECCCCCCEEECCEEEECCCCCCEEEEHEECCCCCCCCCCCCCCEEEEECCC
GRIGILIGHDAVFPEAGRVLALRGCDLIVCPSAVKGIFSAAHAGTKVMQPSPIPTGADPY
CEEEEEEECCCCCCCCCCEEEEECCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HWHHFRVRGGENNAYFAFANVCDAADEDCGLSGVFGPDTFAFPRREAIVDRGEGIATLEI
EEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEE
DTGNLDSVYPTNVVRRKDLVCMRMPHSYRALVQSKASNF
CCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA